• Title/Summary/Keyword: 이수체

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Biodegradation of Phthalic acid by White rot Fungus, Polyporus brumalis (백색부후균 Polyporus brumalis에 의한 프탈산의 분해)

  • Lee, Soo-Min;Park, Ki-Ryung;Lee, Sung-Suk;Kim, Myung-Kil;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.1 s.129
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    • pp.48-57
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    • 2005
  • Phthalate esters are known as plasticizers and some of them suspected as endocrine disrupting chemicals. In this study, in order to identify the mechanism of phthalate esters degradation by white rot fungus, phthalic acid, which is major metabolite in the biodegradation of phthalate esters, was used. Phthalic acid 50 ppm was treated in culture medium with Polyporus brumalis. The availability of ABTS oxidation was different from control and phthalic acid treated group after 4 days of incubation. The activity was gradually increased in control group, but not in phthalic acid treated group. Especially, esterase activity of control group was maximized at 10 days of incubation, and then decreased while the activity of phthalic acid treated group was increased. Glucose was used as a carbon source, and the difference of glucose consumption by control and phthalic acid treated group was not significant. However, after 6 days of incubation the residual glucose in culture medium was rapidly decreased. The consumption rate of phthalic acid treated group was lower than control. These results might indicate that the absorption of phthalic acid in culture medium was occurred by mycelium and metabolized through some pathways as that of glucose was. To clearify the chemical modification of phthalic acid in culture medium, phthalic acid was reacted under in vitro condition which mycelium was excluded. The metabolites were analyzed by GC/MS. The results showed that phthalic acid was converted to phthalic acid anhydride by the extracellular enzymes of P. brumalis.

Analytical Determination of Cyanide in Maesil (Prunus mume) Extracts (매실추출제품의 시안화합물 분석법에 관한 연구)

  • Kim, Eun-Jung;Lee, Hwee-Jae;Jang, Jin-Wook;Kim, In-Young;Kim, Do-Hyeong;Kim, Hyun-Ah;Lee, Soo-Min;Jang, Ho-Won;Kim, Sang-Yub;Jang, Young-Mi;Im, Dong-Kil;Lee, Sun-Hee
    • Korean Journal of Food Science and Technology
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    • v.42 no.2
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    • pp.130-135
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    • 2010
  • Picrate, enzyme-picrate and instrumental analysis methods using IC (Ion Chromatography) and HPLC (High Performance Liquid Chromatography) were compared for their effectiveness in determining cyanide in extracts of Maesil, which is classified as a harmful substance. First, the picrate method showed the shortest analysis time (about 5 hr). The color of picrate paper changed at 0.01 mg/$200\;mL\;CN^-$. However, it was difficult to detect cyanide from amygdalin of glucosides. Second, we performed a qualitative analysis for total cyanide (free cyanide and cyanide from amygdalin) by the enzyme-picrate method using $\beta$-glucosidase and a quantitative analysis by spectrophotometry. Finally, analysis of cyanide by IC and HPLC required the longest determining time (about 17 hr) as well as pretreatment for each free cyanide and amygdalin. These results suggest that enzyme-picrate is the most effective analysis method for the detection of cyanide in Maesil extracts.

A Study on the Cultural Landscape Characteristics of Village Forest Sites in the Southern Coast - A Case Study of Namhae-Goon and Tongyoung City - (경상남도 남해안 마을숲의 문화경관 특성 연구- 남해군과 통영시를 중심으로 -)

  • Lim, Eui-Je;So, Hyun-Su;Lee, Soo-Dong
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.30 no.3
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    • pp.29-41
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    • 2012
  • This study's objective is to inform people of the value and identity and to understand the cultural landscape characteristics of coastal village forest, which have not been studied in-depth in previous studies. This study was conducted by selecting 10 village forest sites in the southern coast of Gyeongsangnam-do, Korea and the present state of forests were investigated through bibliographical and on-the-spot survey, as well as interviews with local villagers. The following points(characteristics of traditional culture, practical characteristics, scenic characteristics) are the important factors for analyzing cultural landscape characteristics of coastal village forest verified through the study. First, The coastal village forest in the village commons is mental space for the local community affected by folk belief rather than confucian backgrounds. Second, The coastal village forest is a reasonable space contriving economic efficiency and solving the real problems facing the coastal village. Third, The coastal village forest identities should be understood the land use around village and behind mountain from the sea of wide range rather than confined to just a forest consisting of trees. This study's significance is to make the basic data and to enhance the value of the coastal village forest unnoticed. In addition, the subjects of the study should be extended to the east and west coastal village forests and subsequent research should be conducted to notification the characteristics and differences of each coastal village forest.

Effects of Drying Method on N-Nitrosamine Formation in Squid during Its Drying (오징어의 건조방법이 N-Nitrosamine의 생성에 미치는 영향)

  • 성낙주;이수정;신정혜;김정균
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.4
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    • pp.614-619
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    • 1997
  • To examine effects of drying methods on the formation of N-nitrosamine(NA) in squid during its drying. Three different types of dried products, which were made by sun, hot-air and traditional drying of squid after removal of intestines, were utilized. NA was analyzed by a gas chromatography-thermal energy analyzer. The contents of nitrate and nitrite nitrogen in dried products were in the range of 0 to 5.2mg/kg and 0 to 2.7mg/kg, respectively. The contents of TMAO and betain nitrogen in squid during its drying decreased, while those of TMA and DMA nitrogen increased. The contents in dried products were 27.8~29.2mg% and 10.4~12.3mg/kg, respectively. N-Nitrosodimethylamine (NDMA) was only detected in squid during its drying, recovery from raw sample and its dried pro- ducts spiked with 10$\mu\textrm{g}$/kg for NDPA was 81.0~ 100.0%. NDMA in raw samples was found to be below 0.05$\mu\textrm{g}$/kg. The levels of NDMA in squid increased remarkably during its drying and those in dried products were ranged from 2.7 to 42.7$\mu\textrm{g}$/kg. The effects of drying methods in squid were found to be quite different. the levels of NDMA in traditionally dried products were 11~16 and 3~4 times higher than those in sun dried and hot-air dried products, respectively It is believed that high levels of NDMA were detected in traditionally dried products because NDMA was formed from reaction between various amines and nitrogen oxide produced by combustion of briquet during drying of squid.

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Solubilization of Arabinogalactan by Extrusion from Portulaca oleracea L. and Its In Vitro Antioxidant Activity (마치현 아라비노갈락탄의 압출 수용화와 항산화 특성)

  • Choi, Ae-Jin;Jee, Ho-Kyun;Ko, Bo Sung;Kim, Yangha;Lee, Soo-Jeong;Kim, Chul-Jin;Cho, Yong-Jin;Kim, Chong-Tai
    • Food Engineering Progress
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    • v.13 no.3
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    • pp.169-175
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    • 2009
  • Water soluble polysaccharides (WSP) and arabinogalactan of Portulaca oleracea L. (POL) were increased after extrusion and commercial cellulase treatment. Arabinose and galactose content increased more about 1.5 times than those of raw POL, and rhamnose also increased about 2.6 times in WSP. High molecular weight fraction (I) of POL depending on extrusion condition including Ext I, Ext II and Ext III degraded into low molecular weight fraction (II) about 37, 29, and 26%, respectively, ranged from 67,000-69,000 Da of molecular weight. Especially, the molecular weight and composition of WSP with extruded, were increased from 9 to 13% in low molecular weight fraction, compared to those of raw POL. Solubilization and degradation of polysaccharides were a directly propotional to specific mechanical energy in POL extrusion. WSP obtained by extrusion at Ext I and Ext II were found to be effective antioxidants in different in vitro assays with regards to 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC). However, these results suggest that WSP obtained using extrusion and subsequent enzymatic treatment may be an effective method to produce arabinogalactan from POL and be used as a functional food ingredients.

Effect of Different Cultivation Systems on Soil Glomalin Content and Nutrient Uptake of Strawberry in Controlled Horticultural Land (시설 딸기 재배형태가 토양 글로말린 함량과 양분흡수량에 미치는 영향)

  • Min, Se-Gyu;Lee, Seung-Ho;Nam, Sang-Hoe;Choi, Yong-Uk;Lee, Su-Yeol;Park, Su-Seon;Lee, Seong-Tae;Kim, Eon-Seok;Song, Won-Doo;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.3
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    • pp.452-456
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    • 2011
  • Glomalin has important roles in soil aggregation in agricultural lands including controlled horticultural lands. The objective of this study was to measure total glomalin content of soils treated conventional farming system (CFS), conventional farming system without pesticides (CFSWP), and organic farming system (OFS) for strawberry cultivation under greenhouse in Goseong-gun, Korea. The average concentration of total glomalin in the soils was significantly higher in the OFS ($2.00mg\;g^{-1}$) compared to the CFS ($1.68mg\;g^{-1}$). In addition, soil microbial biomass C content was 4.9 times higher in the OFS ($821mg\;kg^{-1}$) compared to the CFS ($169mg\;kg^{-1}$). Nitrogen uptake rate of strawberry was higher in the OFS (52.4%) than that in the CFS (13.0%). Furthermore, yield of strawberry in OFS ($51Mg\;ha^{-1}$) was significantly higher compared to CFS ($35Mg\;ha^{-1}$).

An Experimental Study on the Reduction Effects of Shading Devices on Sky Radiant Cooling in Winter (차양장치의 겨울철 천공복사 냉각 저감 효과에 관한 실험적 연구)

  • Kim, Jin-Hee;Kim, Young-Tag;Lee, Soo-Yeol;Choi, Won-Ki
    • Land and Housing Review
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    • v.12 no.1
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    • pp.129-137
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    • 2021
  • External shading devices are well known solar control devices that can help reduce the cooling load of commercial buildings. For this study, experiments were conducted to examine the feasibility of shading devices in reducing both the cooling and heating loads. The influence of sky radiant cooling during winter was verified for the external shading device, internal roller blind, and window. Results can be summarized as follows. The temperature difference between the inner and outer surfaces of the window with the external shading device was 11.8℃ compared to 14.6℃ for one without the external shading device. This 2.8℃ difference was due to heat exchange by sky radiation when the surface temperature of the shading device was lower than the ambient outdoor air temperature. The roller blind resulted in a lower temperature of 0.8℃ compared to the average temperature of the window's air cavity. This was due to heat exchange by sky radiation of the roller blind surfaces. Without shading devices, the outside surface temperature of the window is about 3℃ higher. The study also found that when external shading devices were installed on both the southwest and southeast sides, the outside surface temperature of the windows were lower on the southwest side than the southeast side.

The Continuance and Changes of Horsehair Handicraft Tradition as Intangible Cultural Heritage (무형문화유산으로서 말총공예 전통의 지속과 변화)

  • Hwang, Kyeong-soon
    • Korean Journal of Heritage: History & Science
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    • v.47 no.3
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    • pp.160-171
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    • 2014
  • The provision of support for the art of making these items needs to be considered with the focus on the following factors: the local situation of the areas where such traditional handicrafts are still made, the craftspeople involved in their production, and their communities. So far, discussion about how to reinvigorate traditional handicrafts, including those mentioned above, has been concentrated on the measures taken to promote them as part of the handicrafts industry and the allocation of the government's budget for important intangible cultural heritages. The government runs a traditional handicrafts management system and provides financial support for the craftspeople and spaces for exhibiting their work. This form of support has led to systematic management of traditional handicrafts and heightened public interest in cultural heritage, as well as publicizing the country's traditional crafts, but has made little progress in the following areas: the fostering of young people willing to learn traditional skills, diversification of the types of skills to be maintained, or establishment of the networks of collaboration among the craftspeople. The most important aspect among the efforts mentioned above is to maintain cultural traditions that are unique to each region by encouraging local craftspeople to engage in their work with a solid sense of pride backed up by financial support. This study was carried out in connection with the need to reinvigorate the art of making tanggeon (horsehair crown), manggeon (horsehair headband), and gat (black horsehair hat), which few people wear as they are used only for ornamental purposes nowadays. This study examined the circumstances surrounding the artisans engaged in the production of horsehair handicrafts prior to their designation as a cultural heritage, and the changes that occurred in the local communities associated with their production after the designation, in order to assess the status of inheritance of this tradition.

Development of specific single nucleotide polymorphism molecular markers for Angelica gigas Nakai (ITS 영역의 HRM 분석을 통한 참당귀(Angelica gigas Nakai)의 특이적 SNP 분자표지 개발)

  • Lee, Shin-Woo;Lee, Soo Jin;Han, Eun-Hee;Shin, Yong-Wook;Kim, Yun-Hee
    • Journal of Plant Biotechnology
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    • v.48 no.2
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    • pp.71-76
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    • 2021
  • Angelica is a perennial plant used widely for medicinal purposes. Information on the genetic diversity of Angelica populations is important for their conservation and germplasm utilization. Although Angelica is an important medicinal plant genus registered in South Korea, no molecular markers are currently available to distinguish individual species from other similar species in different countries, in particular, China and Japan. In this study, we developed single nucleotide polymorphism (SNP) markers derived from internal transcribed spacer regions of the nuclear ribosomal DNA to identify a distinct domestic species, Angelica gigas Nakai, via a high-resolution melting (HRM) curve analyses. We also performed HRM curve analysis of intentionally mixed genomic DNA samples from five Angelica species. Finally, we investigated A. gigas Nakai and A. sinensis using varying ratios of mixed genomic DNA templates. The SNP markers developed in this study are useful for rapidly identifying A. gigas species from different countries.

A Study on the Design of Prediction Model for Safety Evaluation of Partial Discharge (부분 방전의 안전도 평가를 위한 예측 모델 설계)

  • Lee, Su-Il;Ko, Dae-Sik
    • Journal of Platform Technology
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    • v.8 no.3
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    • pp.10-21
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    • 2020
  • Partial discharge occurs a lot in high-voltage power equipment such as switchgear, transformers, and switch gears. Partial discharge shortens the life of the insulator and causes insulation breakdown, resulting in large-scale damage such as a power outage. There are several types of partial discharge that occur inside the product and the surface. In this paper, we design a predictive model that can predict the pattern and probability of occurrence of partial discharge. In order to analyze the designed model, learning data for each type of partial discharge was collected through the UHF sensor by using a simulator that generates partial discharge. The predictive model designed in this paper was designed based on CNN during deep learning, and the model was verified through learning. To learn about the designed model, 5000 training data were created, and the form of training data was used as input data for the model by pre-processing the 3D raw data input from the UHF sensor as 2D data. As a result of the experiment, it was found that the accuracy of the model designed through learning has an accuracy of 0.9972. It was found that the accuracy of the proposed model was higher in the case of learning by making the data into a two-dimensional image and learning it in the form of a grayscale image.

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