• Title/Summary/Keyword: 약충

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Characterization of Lecanicillium lecanii Btab0l Isolated with Bioactivities to Tabacco Whitefly (Bemisia tabaci) (담배가루이(Bemisia tabaci) 병원성곰팡이 Lecanicijjium lecanii Btab0l 균주의 특성)

  • Yoon, Yeo-Jun;Yu, Yong-Man;Lee, Min-Ho;Han, Eun-Jung;Hong, Sung-Jun;Ahn, Nan-Hee;Kim, Yong-Ki;Jee, Hyung-Jin;Park, Jong-Ho
    • Korean journal of applied entomology
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    • v.49 no.4
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    • pp.417-422
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    • 2010
  • Cultural characteristics Lecanicillium lecani Btab01 and its insecticidal activity against tobacco whitefly (Bemisia tabaci) were investigated. On potato dextrose agar, tryptic soy agar and SDA+Y media, mycelial growth of L. lecani Btab01 was best at $20{\sim}25^{\circ}C$ and suppressed above $28^{\circ}C$. Both solid culture and liquid culture of L. lecani Btab01 showed high insecticidal activity, 93.9 and 98.3% respectively, against nymph of tobacco whitefly, but there is no significant difference. When culture of L. lecani Btab01 was treated at the concentration of $10^5$, $10^6$, $10^7$ and $10^8$ cfu/ml, their insecticidal activity were 5.8%, 33.8%, 77.3% and 98.5% respectively, and $LT_{50}$ values were 16.1 days, 7.3 days, 5.1 days and 3.5 days respectively. When nymphs were treated by the cultures of L. lecani Btab01 and maintained under saturated condition for zero hour, 24 hours and 168 hours, their control activities were 0%, 20.3% and 100% respectively. Spore germination of L. lecani Btab01 was increased about two times by adding edible oil. When L. lecani Btab01 was treated to control nymph with 0.1% edible oil, it showed high control activity(98.6%) compared to single treatment of L. lecani Btab01 (79.9%).

Comparison of Development times of Myzus persicae (Hemiptera:Aphididae) between the Constant and Variable Temperatures and its Temperature-dependent Development Models (항온과 변온조건에서 복숭아혹진딧물의 발육비교 및 온도 발육모형)

  • Kim, Do-Ik;Choi, Duck-Soo;Ko, Suk-Ju;Kang, Beom-Ryong;Park, Chang-Gyu;Kim, Seon-Gon;Park, Jong-Dae;Kim, Sang-Soo
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.431-438
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    • 2012
  • The developmental time of the nymphs of Myzus persicae was studied in the laboratory (six constant temperatures from 15 to $30^{\circ}C$ with 50~60% RH, and a photoperiod of 14L:10D) and in a green-pepper plastic house. Mortality of M. persicae in laboratory was high in the first(6.7~13.3%) and second instar nymphs(6.7%) at low temperatures and high in the third (17.8%) and fourth instar nymphs(17.8%) at high temperatures. Mortality was 66.7% at $33^{\circ}C$ in laboratory and $26.7^{\circ}C$ in plastic house. The total developmental time was the longest at $14.6^{\circ}C$ (14.4 days) and shortest at $26.7^{\circ}C$ (6.0 days) in plastic house. The lower threshold temperature of the total nymphal stage was $3.0^{\circ}C$ in laboratory. The thermal constant required for nymphal stage was 111.1DD. The relationship between developmental rate and temperature was fitted nonlinear model by Logan-6 which has the lowest value on Akaike information criterion (AIC) and Bayesian information criterion (BIC). The distribution of completion of each developmental stage was well described by the 3-parameter Weibull function ($r^2=0.95{\sim}0.97$). This model accurately described the predicted and observed occurrences. Thus the model is considered to be good for use in predicting the optimal spray time for Myzus persicae.

Comparison of Temperature-dependent Development Model of Aphis gossypii (Hemiptera: Aphididae) under Constant Temperature and Fluctuating Temperature (실내 항온과 온실 변온조건에서 목화진딧물의 온도 발육비교)

  • Kim, Do-Ik;Ko, Suk-Ju;Choi, Duck-Soo;Kang, Beom-Ryong;Park, Chang-Gyu;Kim, Seon-Gon;Park, Jong-Dae;Kim, Sang-Soo
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.421-429
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    • 2012
  • The developmental time period of Aphis gossypii was studied in laboratory (six constant temperatures from 15 to $30^{\circ}C$ with 50~60% RH, and a photoperiod of 14L:10D) and in a cucumber plastic house. The mortality of A. gossypii in the laboratory was high in the 2nd (20.0%) and 3rd stage(13.3%) at low temperature but high in the 3rd (26.7%) and 4th stage (33.3%) at high temperatures. Mortality in the plastic house was high in the 1st and 2nd stage but there was no mortality in the 4th stage at low temperature. The total developmental period was longest at $15^{\circ}C$ (12.2 days) in the laboratory and shortest at $28.5^{\circ}C$ (4.09 days) in the plastic house. The lower threshold temperature at the total nymphal stage was $6.8^{\circ}C$ in laboratory. The thermal constant required to reach the total nymphal stage was 111.1DD. The relationship between the developmental rate and temperature fit the nonlinear model of Logan-6 which has the lowest value for the Akaike information criterion(AIC) and Bayesian information criterion(BIC). The distribution of completion of each development stage was well described by the 3-parameter Weibull function ($r^2=0.89{\sim}0.96$). This model accurately described the predicted and observed outcomes. Thus it is considered that the model can be used for predicting the optimal spray time for Aphis gossypii.

Effect of Cold Storage on Quality of Geocoris pallidipennis and Micromus angulatus (저온저장이 포식성 천적인 참딱부리긴노린재와 갈고리뱀잠자리붙이의 품질에 미치는 영향)

  • Seo, Meeja;Kim, Jeong Hwan;Seo, Bo Yoon;Park, Bueyong;Lee, Sang Gu;Kim, Kwang Ho;Cho, Jum Rae
    • Korean journal of applied entomology
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    • v.58 no.2
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    • pp.151-157
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    • 2019
  • It was available to store both nymphs and adults of Geocoris pallidipennis at $5^{\circ}C$ for 3 weeks without negative effects on their survival, fecundity, and Bemisia tabaci predation. Fecundities of G. pallidipennis showed the tendency to be decreased with increasing cold storage duration, but up to 4 weeks, storage at $5^{\circ}C$ didn't affect their fecundity. When Micromus angulatus adults were stored at $5^{\circ}C$ with honey solution as preys, it was possible to store up to 140 days showing approximately 50% of cumulative survival, but over 2 week cold storage was not suitable for their fecundity. With the increase of cold storage duration, the aphid predation by M. angulatus decreased, but it could be possible to cold store until 5 weeks without any significant effect on their aphid predation. Thus, short term cold storage of G. pallidipennis and M. angulatus could be useful for inundative biological strategies.

Comparative Analysis of Cold Tolerance and Overwintering Site of Two Flower Thrips, Frankliniella occidentalis and F. intonsa (꽃노랑총채벌레와 대만총채벌레의 내한성과 월동처 비교 연구)

  • Chulyoung, Kim;Du-yeol, Choi;Falguni, Khan;Md Tafim Hossain, Hrithik;Jooan, Hong;Yonggyun, Kim
    • Korean journal of applied entomology
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    • v.61 no.3
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    • pp.409-422
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    • 2022
  • Two dominant thrips in hot pepper (Capsicum annuum) cultivating in greenhouses are Frankliniella occidentalis and F. intonsa in Korea. This study investigated their overwintering physiology. These two thrips were freeze-susceptible and suppressed the body freezing temperature by lowering supercooling point (SCP) down to -15~-27℃. However, these SCPs varied among species and developmental stages. SCPs of F. occidentalis were -25.7±0.5℃ for adults, -17.2±0.3℃ for pupae, and -15.0±0.4℃ for larvae. SCPs of F. intonsa were -24.0±1.0℃ for adults, -27.0±0.5℃ for pupae, -17.2±0.8℃ for larvae. Cold injuries of both species occurred at low temperature treatments above SCPs. Thrips mortality increased as the treatment temperature decreased and its exposure period increased. F. occidentalis exhibited higher cold tolerance than F. intonsa. In both species, adults were more cold-tolerant than larvae. Two thrips species exhibited a rapid cold hardening because a pre-exposure to 0℃ for 2 h significantly enhanced the cold tolerance to a lethal cold temperature treatment at -10℃ for 2 h. In addition, a sequential exposure of the thrips to decreasing temperatures made them to be acclimated to low temperatures. To investigate the overwintering sites of the two species, winter monitoring of the thrips was performed at the greenhouses. During winter season (November~February), adults of the two species were not captured in outside of the greenhouses. However, F. occidentalis adults were captured to the traps and observed in weeds within the greenhouses. F. occidentalis adults were also emerged from soil samples obtained from the greenhouses during the winter season. F. intonsa adults did not come out from the soil samples at November and December, but emerged from the soil samples obtained after January. To determine the adult emergence due to diapause development, two thrips species were reared under different photoperiods. Adult development occurred in all photoperiod treatments in F. occidentalis, but did not in F. intonsa especially under short periods. Tomato spotted wilt virus, which is transmitted by these two species, was detected in the weeds infested by the thrips during the winter season. These results suggest that F. occidentalis develops on weeds in the greenhouses while F. intonsa undergoes a diapause in the soil during winter.

Scanning Electron Microscopy on Ixodes signatus Nymphs with Particular Reference to Major Physiological Sensory Organs (Ixodes signatus 약충(若蟲)의 생리학적(生理學的) 주요(主要) 감각기관(感覺器官)에 대(對)한 주사전자현미경적(走査電子顯微鏡的) 관찰(觀察))

  • Kang, Yung-bai
    • Korean Journal of Veterinary Research
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    • v.28 no.1
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    • pp.145-154
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    • 1988
  • Ixodes signatus nymphs were subjected to the scanning electron microscopy for the observation of the major physiological sense organs including the Hailer's organ. Finger prints and variable sensory setae were found on the body surface, and especially well developed setae were identified on the article IV and on the anal lobes. A central button-like organelle and numerous small holes were found in the spiracular plate. The Haller's organ was identified at the distal point of the tarsus I and regarded as the major sensory organ, chemoreceptor. Tow types of sensory setae were observed, the shorter ones in front of the organ and the longer ones in distal front as well as near behind of the organ. The fully opened organ was ellipsoidal and a total of seven basiconic sensory pegs rooted deeply and shaped as well arranged hook-like was found inside the organ.

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Host Preference by the Small Brown Planthopper and Green Rice Leafhopper on Barley and Water Foxtail (I) (보리와 둑세풀에 대한 애멸구$\cdot$끝동매미충의 기주 선택성(제1보))

  • Choi S. Y.;Lee H. R.
    • Korean journal of applied entomology
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    • v.15 no.4 s.29
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    • pp.179-184
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    • 1976
  • Laboratory experiments were conducted to clarity the early-spline host·selectivity by the small brown planthopper(SBPH), Laodelphax striatellus Fallen, and green rice Leafhopper(GLH), Nephotettix cincticeps Uhle., on the bailey (the variety Suweon #18) and water foxtail (Alopeculus aequadis Sosbol). The host selectivity was evaluated on the bases of feeding and ovipositional perferences of the insects on the plants and their biological effects on the plants. The nymphs of SBPH much more preferred barley for feeding than water forxtail, while the nymphs of GLH relatively prefered water foxtail. There was no significant difference in ovipositional preference by SBPH among the test plants, and ovipositional preference by GLH Ivas significantly lower on barley and water foxtail than on rice. Nymphal growth an4 adult emergence of SBPH were significantly faster and higher on barley than on water foxtail. No adult emergence of GLH was observed on barley, and adult emergence was still quite lower even on water foxtail. The adult of SBPH fed on barley showed longer longevity aad higher fecundity than that of SBPH from water foxtail and rice. The adult GLH fed ell water foxtail shorted relatively shorter longevity and to)ver fecundity than that of GLH on rice. In conculsion, barley seems to be more adequate for spring host of SBPH than water foxtail, but barley may not be quite adequate for spring host of GLH. At presont moment, GLH seems rather to primarily select the water foxtail than barley as a spring host, even if the water foxtail is not so adequate for development of GLH.

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Studies on the Life History of Nysius plebejus Distant (Hemiptera: Lygaeidae) an Insect Pest of Chrysanthemum (국화 해충의 1종인 애긴노린재의 생활사에 관한 연구)

  • 김정부;강달순;김태성;신원교;이유식
    • Korean journal of applied entomology
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    • v.33 no.2
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    • pp.56-59
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    • 1994
  • Life histoy of Nysius plebejrrs, the most injurious pest in chysanthemun cultivation in Korea, was studies both in field and inseday in Chinju during 1991 and 1992 seasons, and resulis are summarized as below 7hls insect has 3 generation per year, adult peaks on the mid April, mid June and late August, and the highest number of adults were attracted to the light tmp from the late August through mid September. Overwntered generation and needed 6-7weeks from egg rnatulity m spring, and 5-6 weeks, for both the first and second generation m summer, Incubation egg periods were 142, 9.3 and 7.2 days, nymphal penods were 45.2, 32.1 and 31.2 days, and total growth periods, were 59.4. 41 4 and 38.4 days, respect~vely for the overwintered, flst and second generation. Ovipositional penods were 25.6, 23.4 and 21.2 days. and number of eggs Iald by each female were 41.5. 37.2 and 35.4 days, respedively of each generation Longevities of female were 326, 27.5 and 25.1 days. and of male were 15 2, 12.3 and 12.1 days. respectively of each generation. Sex ratlo was about 1:1 in all generation, and eggs were laid in mass on the surface of leaves or stems of the host plants. This insed over-wmters as adult (90%) or 5th instar nymph (10%), however, over-wintering adults seem to be sexually premature, and mating take place actively in the following spring.

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Varietal Resistance of Rice to the Green Rice Leafhopper, Nephotettix cincticeps Uhler (끝동매미충에 대한 벼의 저항성 및 그 기작에 관한 연구)

  • Choi Seung Yoon
    • Korean journal of applied entomology
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    • v.14 no.1 s.22
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    • pp.13-21
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    • 1975
  • Experiments were conducted to select the new varieties and or/lines of rice resistant to the green rice leafhopper, Nephotettix cincticeps Uhler, and to evaluate the nature of their resistance to the insects in connection with the antibiosis and feeding and ovipositional preferences. The materials tested in this study were the varieties and or/lines of rice mostly proposed by the International Insect Resistance Nursery of IRRI and some others also orginiated from IRRI. Out of the 48 varieties and or/lines the 9 were selected as resistant and the 11 as mderately resistant. They have high antibiosis to the insects, showing significantly higher nymphal mortality and less adult emergence in the resistant and moderately resistant ones than the susceptible and moderately susceptible varieties Jinheung and Tong-il. Feeding perference was significantly different between the resistant and susceptible rice plants, resulting in much lower on resistant ones and much higher on susceptible ones. Ovipositional preference, however, was not different at all between the resistant and the susceptible ones. The nature of varietal resistance of rice to the green rice leafhopper seemed to be surely associated with the antibiosis and non-feeding preference.

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Chemical Control Effect Against Spot Clothing Wax Cicada, Lycorma delicatula (Hemiptera: Fulgoridae) Nymphs and Adults (꽃매미(Lycorma delicatula) 약충과 성충에 대한 약제방제 효과)

  • Kim, Sun-Kook;Lee, Gi-Yeul;Shin, Yon-Ho;Kim, Gil-Bah
    • The Korean Journal of Pesticide Science
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    • v.14 no.4
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    • pp.440-445
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    • 2010
  • This study was carried out to investigate the toxicities of five registered insecticides to the Lycorma delicatula nymphs and adults. Insecticidal activities were evaluated by testing systemic and residual effects in the laboratory, and control efficacy in the vineyard. For the 3rd nymphal instars and adults, etofenprox+diazinon, chlorpyrifos, etofenprox, dinotefuran and imidacloprid showed perfect insecticidal activity. The systemic effects of dinotefuran and etofenprox+diazinon on grape roots at half concentration were showed the adult mortality of 82.2% and 84.4%, respectively. Chlorpyrifos at recommended concentration was showed mortality of 86.0%. The leaf systemic effects at recommended and half concentrations of all insecticides except dinotefuran were lower than 65% but the mortality at double concentration of chlorpyrifos, dinotefuran, etofenprox were more than 82%. The residual effect between etofenprox+diazinon and dinotefuran at recommended and double concentrations were 100% at 14 days after treatment, the other insecticides have low efficacy. All the four insecticides showed 87% control value on nymphs in the field test and adults appeared more than 96% control value st and adinotefuran, etofenprox+diazinon, however, the other insecticides decreased to 59.1 % and 61.2%. Therefore, dinotefuran and etofenprox+diazinon showing high systemic effects to roots and long residual effects to leafs have high control efficacies.