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Characterization of Lecanicillium lecanii Btab0l Isolated with Bioactivities to Tabacco Whitefly (Bemisia tabaci)

담배가루이(Bemisia tabaci) 병원성곰팡이 Lecanicijjium lecanii Btab0l 균주의 특성

  • Yoon, Yeo-Jun (Institute of Biotechnology, Koreabio co., ltd.) ;
  • Yu, Yong-Man (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Lee, Min-Ho (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Han, Eun-Jung (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, Sung-Jun (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ahn, Nan-Hee (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Yong-Ki (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jee, Hyung-Jin (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Jong-Ho (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration)
  • 윤여준 (고려바이오(주) 부설 농업생명과학기술연구소) ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 이민호 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 한은정 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 홍성준 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 안난희 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 김용기 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 지형진 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 박종호 (농촌진흥청 국립농업과학원 유기농업과)
  • Received : 2010.11.17
  • Accepted : 2010.12.15
  • Published : 2010.12.30

Abstract

Cultural characteristics Lecanicillium lecani Btab01 and its insecticidal activity against tobacco whitefly (Bemisia tabaci) were investigated. On potato dextrose agar, tryptic soy agar and SDA+Y media, mycelial growth of L. lecani Btab01 was best at $20{\sim}25^{\circ}C$ and suppressed above $28^{\circ}C$. Both solid culture and liquid culture of L. lecani Btab01 showed high insecticidal activity, 93.9 and 98.3% respectively, against nymph of tobacco whitefly, but there is no significant difference. When culture of L. lecani Btab01 was treated at the concentration of $10^5$, $10^6$, $10^7$ and $10^8$ cfu/ml, their insecticidal activity were 5.8%, 33.8%, 77.3% and 98.5% respectively, and $LT_{50}$ values were 16.1 days, 7.3 days, 5.1 days and 3.5 days respectively. When nymphs were treated by the cultures of L. lecani Btab01 and maintained under saturated condition for zero hour, 24 hours and 168 hours, their control activities were 0%, 20.3% and 100% respectively. Spore germination of L. lecani Btab01 was increased about two times by adding edible oil. When L. lecani Btab01 was treated to control nymph with 0.1% edible oil, it showed high control activity(98.6%) compared to single treatment of L. lecani Btab01 (79.9%).

국내에 문제가 되는 담배가루이(Bemisia tabaci)의 살충성 Lecanicillium lecanii Btab01 균주의 생육 특성과 살충력을 검정하였다. PDA, TSA, SDA+Y배지에서 L. lecanii Btab01은 $20{\sim}25^{\circ}C$에서 균사성장이 가장 빨랐으며 $28^{\circ}C$이상에서 성장이 억제되었다. L. lecanii Btab01는 고체배양과 액체배양 시 담배가루이 약충에 대해 살충률에 큰 차이를 보이지는 않았다(93.9%, 98.3%). 농도별로는 $10^5$, $10^6$, $10^7$, $10^8$ cfu/ml에서 각각 5.8%, 33.8%, 77.3%, 98.5%의 살충률을 보였고 각각 16.1일, 7.3일, 5.1일, 3.5일의 $LT_{50}$값을 나타내었다. L. lecanii Btab01을 약충에 처리 후 포화습도 조건을 0 시간 24 시간, 168 시간 유지하였을 때, 0%, 20.3%, 100%의 살충률을 보였다. 균주에 0.5% 식용유를 첨가하였을 때 포자의 발아가 2 배 가량 증가하였으며 균주에 0.1% 식용유를 첨가하여 약충에 처리했을 때(98.6%)가 첨가하지 않았을 때(79.9%)보다 살충력이 향상됨을 알 수 있었다.

Keywords

References

  1. Barranco-Florido, J.E., R. Alatorre-Rosas, M. Gutidrrez-Rojas and G. Viniegra-Gonzalez. 2002. Criteria for the selection of strains of entomo-pathogenic fungi Verticillium lecanii for solid state cul-tivation. Enzyme Microb. Technol. 30: 910-915. https://doi.org/10.1016/S0141-0229(02)00032-7
  2. Chandler, D., B. Heale and A.T. Gillespie. 1993. Germination of the entomo-pathogenic fungus Verticillium lecanii on scales of the glass-house whitefly Trialeurodes vaporariorum. Biocontrol Sci. Technol. 3: 161-164. https://doi.org/10.1080/09583159309355272
  3. Drummond, J., J.B. Heale and A.T. Gillespie. 1987. Germination and effect of reduced humidity on expression of pathogenicity in Verticillium lecanii against the glasshouse whitefly Trialeurodes vaporariorum. Ann. Appl. Biol. 111: 193-201. https://doi.org/10.1111/j.1744-7348.1987.tb01446.x
  4. Faria, M. and Wraight SP. 2001. Biological Control of Bemisia tabaci with fungi. Crop Protect. 20: 767-778. https://doi.org/10.1016/S0261-2194(01)00110-7
  5. Hall, R.A. 1981. A new insecticide against greenhouse aphids and whitefly: the fungus Verticillium lecanii. Ohio Florists' Assoc. Bull. 626: 3-4.
  6. Hall, R.A. 1982. Control of whitefly Trialeurodes vaporariorum and cotton aphid Aphis gossypii in glasshouses by two isolates of the fungus Verticillium lecanii. Ann. App. Biol. 101: 1-11. https://doi.org/10.1111/j.1744-7348.1982.tb00794.x
  7. Kanagaratnam, P., R.A. Hall and H.D. Burges. 1981. New or unusual records of plant diseases and pests. Plant Pathol. 30: 117-118. https://doi.org/10.1111/j.1365-3059.1981.tb01238.x
  8. Kim, E.H., J.W. Sung, J.O. Ahn, H.G. Ahn, C.M. Yoon, M.J. Seo and G.H. Kim. 2007. Comparison of insecticide susceptibility and enzyme activities of biotype B and Q of Bemisia tabaci. Kor. J. Pesticide Sci. 11(4): 320-330.
  9. Lee, I.K., H.J. Shim, S.D. Woo and S.K. Kang. 1997. Selection of entomopathogenic fungi to green house whitefly, Trialeurodes vaporariorum. Kor. J. Pest. Sci. 1(1): 13-17.
  10. Lee, M.H., S.Y. Kang, S.Y. Lee, H.S. Lee, J.T Choi, G.S. Lee, W.Y. Kim, S.W. Lee, S.G. Kim and K.B. Uhm. 2005. Occurrence of the B- and Q-biotypes of Bemisia tabaci in Korea. Kor. J. Appl. Entomol. 44(3): 169-175.
  11. Lee, M.L., S.B. Ahn and W.S. Cho. 2000. Morphological Characteristics of Bemisia tabaci(Gennadius) (Homoptera: Aleyrodidae) and Discrimination of Their Biotypes in Korea by DNA Makers. Kor. J. Appl. Entomol. 39(1): 5-12
  12. Milner, R.J. and G.G. Lutton. 1986. Dependence of Verticillium lecanii (Fungi: Hyphomycetes) on high humidities for infection and sporulation using Myzus persicae (Homoptera: Aphididae) as host. Environ. Entomol. 15: 380-382. https://doi.org/10.1093/ee/15.2.380
  13. Osborne, L.S. and Z. Landa. 2003. Biological control of whiteflies with ento-mopathogenic fungi. Florida Entomologist. 75: 456-471.
  14. Perring, T.M., A.D. Cooper, R.J. Rodriguez, C.A. Farrar and T.S. Bellows. 1993. Identification of a whitefly species by genomic and behavioral studies. Sci. 259: 74-77. https://doi.org/10.1126/science.8418497
  15. Perring, T.M. 2001. The Bemisia tabaci species complex. Crop Prot. 20: 725-737. https://doi.org/10.1016/S0261-2194(01)00109-0
  16. Quesada-Moraga, E., E.A.A. Maranhao, P. Valverde-Garcia and C. Santiago-Alvarez. 2006. Selection of Beauveria bassiana isolates for control of the whiteflies Bemisia tabaci and Trialeurodes vaporariorum on the basis of their virulence, thermal requirements, and toxicogenic activity. Biolo. Control. 36: 274-287. https://doi.org/10.1016/j.biocontrol.2005.09.022
  17. Rombach, M.C. and A.T. Gillespie. 1988. Entomogenous Hyphomycetes for insect and mite control on greenhouse crop. Biocontrol News & Information. 9: 7-18.
  18. Shi, Z., M. Li and L. Zhang. 2006. Effects of nutrients on germination of Verticillium lecanii (=Lecanicillium sp.) conidia and infection of green-house whitefly, (Trialeurodes vaporariorum). Biocontrol Sci. Tech. 16(5/6): 599-606. https://doi.org/10.1080/09583150500532832
  19. Zare, R. and W. Gams. 2001. A revision of Verticillium section Protrata. IV. The genera Lecanicillium and Simplicillium. Nova Hedwigia 73: 1-50.

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