• Development and Reproduction
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• v.11 no.2
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• pp.111-119
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• 2007

Differentiation of cells can be induced through modulation of endogenous regulators using exogenous factors. Useful transfection systems to transport a specific exogenous regulator into cell have been tried but still there are many obstacles to overcome. In this study, we examined the transfection efficiency of cell permeable peptides (CPPs) in mouse embryonic stem cell under the various conditions. To identify the CPP-mediated translocation of a protein, we employed recombinant CPP-enhanced green fluorescent protein (EGFP). Viability of R1 cells was different between experimental groups depending on the kind of CPP and the concentration of CPP-EGFP. Translocation of CPP-EGFPs into the R1 cells was not detected until 30 min after CPP-EGFPs treatment in all groups. After 1 hr, translocation of pEP-1-EGFP-N was detected, but it could not be detected in the other group. Transfection of pEP-1EGFP-N was independent on its concentration. The time course did not show saturation even after 24 hr in pEP-1-EGFP-N. These results showed that the permeability depended on the kind of CPP and the location of His-tag in the case of examined CPPs, and did not need biological energy. On summary, the efficiency of transfection of CPP-EGFP depends on the CPP sequences but the culture time is not a key factor in transfection for the mouse embryonic stem cell. For the future studies to improve the efficiency of translocation of protein into embryonic stem cells, it is needed to develop modified CPP or mediator. The studies would be very useful to induce the differentiation of embryonic stem cells.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2004.05b
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• pp.217-220
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• 2004

According as utilization for web increases rapidly, it is demanded that model about support interaction between web-based applications systematically and solutions can integrate new distributed platforms and existing environment effectively, accordingly, Web Services appeared by solution in reply. These days, a lot of software and hardware companies try to adoption of Web Services to their market, attenpt to construct their applications associationing components from various Web Services providers. However, to execute Web Services completely. it must have interoperability and need the standardization work that avoid thing which is subject to platform, application as well as service and programming language from other companies. WS-I (Web Services Interoperability organization) have established Basic Profile 1.0 based on XML, UDDI, WSDL and SOAP for web services interoperability and developed usage scenario Profile to apply Web Services in practice. In this paper, to verify suitability Web Services interoperability between heterogeneous two applications, have design and implements the Book Information Web Services that based on the Web Services Client of J2SE platform and the Web Services Server of .NET platform, so that analysis and verify the service by adaptation of WS-I Basic Profile.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.117-117
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• 2016

일반적인 박막 성장용 CVD는 막 성장 시간이 짧게는 수분에서 수시간 정도 소요하기 때문에 장비에 문제가 발생 할 시 조치를 취하고 다음 현상을 개선하기에 용이 하였다. 그리고 대분분의 장비가 국산화되어 있을 만큼 많은 경험치가 축척되어 있다. 그러나 2, 4 족 화합물 성장용 CVD는 고아학 렌즈 생산용 장비로 국내에서는 아직 생소하고 공정 경험이 없는 새로운 장비이다. 2,4 족 화합물의 특징은 다음과 같다. 2,4 족 화합물은 M, N 이라는 두가 물질이 결합하여 형성한다. 2,4 족 화합물은 높은 융점과 낮은 증기압을 갖니다. 이런 물질들은 고온에서 아래와 같이 평형적으로 반응한다. $$nMN_{(s)}=nM_{(g)}+Nn_{(g)}$$ 화합물인 $MN_{(g)}$의 상태로 존재할 수 있으나 일바적으로 n=2인 4족 원소의 2원자 분자로된 기체가 지배적이다. 증기상을 이용한 성장 공정에서는 구성 원자나 분자를 만들어내는 단계, 이들을 공급원에서 기판까지 수송하는 단계, 기판 위에 흡착하는 단계, 핵의 생성과 단결정을 생성하는 단계, 필요치 않는 구성물을 제거하는 단계를 거쳐 공정이 진행 된다. 각 공정은 성장 물질에 충분한 자유도를 주어야하고 자유도를 주기 위해서는 많은 열에너지가 공급 되어야 한다. 따라서 기존의 박막 성장 공정 보다 성장 속도가 느리고 증착하는 양보다는 버리는 양이 많으며 버려지는 성장물질들은 급격한 온도 변화가 생기는 곳에서 급격히 증착하기 시작한다. 본 성장 공정이 진행되는 압력은 30 torr 부근이며 공정 온도는 $1000^{\circ}C$ 부근이다. 30 torr 영역에서는 열전달이 대기압과 같은 속도로 진행되기 때문에 지속적으로 온도에의해 손상을 받는 부위가 있을 수 있다. 높은 공정 온도와 높은 공정 압력은 내부 구조물로 발생된 열을 빠르게 장비 표면으로 수송하게 되고 그 결과 장비의 연결 부분에 장착된 오링에 손상을 주게 된다. 오링 손상을 방지 하기위해 냉각수 라인을 형성하여 오링을 보호하게 되면 열역학적 기울기가 급격히 발생하는 부분이므로 CVD의 반응 부산물들이 빠른 시간동안 증착하게 되고 막히는 현상이 발생하게 된다. 목표한 두게까지 박막을 성장시키기 위해서는 장시간 공정이 필수이며 장시간 공정을 안정적으로 가져가지 위해서는 배기 라인의 막힘 현상을 해결하여야 한다. 본 논문에서는 막힘 현상의 진행을 시간에 따라 해석하였으며 장시간 공정을 진행하기위해 필요한 요소와 기구적으로 조치가 가능한 방법에 대해 작성하였다.

• The Journal of the Korea Contents Association
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• v.6 no.1
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• pp.114-125
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• 2006

Since wireless Internet services and small mobile communication devices come into wide use as well as the use of GPS is rapidly growing, researches on moving object, whose location information shifts sequently in accordance with time interval, are being carried out actively. Especially, the researches on vehicle moving object are applied to Advanced traveler information system, vehicle tracking system, and distribution transport system. These systems are very useful in searching previous positions, predicted future positions, the optimum course, and the shortest course of a vehicle by managing historical data of the vehicle movement. In addition, vehicle historical data are used for distribution transport plan and vehicle allocation. Vehicle historical data are stored at regular intervals, which can have a pattern. For example, a vehicle going repeatedly around a specific section follows a route very similar to another. If historical data of the vehicle with a repeated route course are stored at regular intervals, many redundant data occur, which result in much waste of storage. Therefore this thesis suggest a vehicle historical data store scheme for vehicles with a repeated route course using similar trajectory which efficiently store vehicle historical data.

• Journal of the Korea Society for Simulation
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• v.22 no.1
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• pp.63-75
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• 2013

The School Bus Routing Problem(SBRP) seeks to plan an efficient schedule of a fleet of school buses that must pick up student from various bus stops and deliver them by satisfying various constraints; maximum capacity of the bus, maximum riding time of students, arrival time between a school's time window. By extending the existing SBRP, we consider a case study of SBRP with allowance of mixed-loading and dynamic arrivals reflecting the school bus operation of university in Korea. Our solution procedure is based on constructing the initial solution using sweep algorithm and then improving solution within the framework of the evolutionary approach known as efficient meta-heuristics. By comparing the various scenarios through the constraints relaxation for reflecting the real operational strategies, we assess the merit of our proposed procedure.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.5
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• pp.501-508
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• 1994

This study was undertaken to get basic data for the cold-waterless transportation of live fish. The optimal cold temperature of plaice, Paralichthy olivaceus was determined by checking the changes of dissolved oxygen and ammonia in the sea water and the survival times during storage at various temperatures. After determination of optimal temperature for transportation, the changes of serum components and muscle components of live plaice were also carried out during storage at cold($5^{\circ}C$)-waterless conditions and the recovery conditions($10\%$ density at $15^{\circ}C$). At higher storage temperature, decreases in dissolved oxygen and the increases in ammonia in seawater were observed. In addition, the survival time was short at low temperature($0^{\circ}C\;and\;3^{\circ}C$). Almost all of the serum components(hemoglobin, glucose, LDH, GOT and GPT) of live plaice gradually increased during storage in cold-waterless conditions, and then those values decreased to the initial levels after $3{\sim}10hrs$ storage in conditions of recovery. The concentration of ATP in the muscle steadily decreased during storage in cold-waterless conditions. The contents of ADP and IMP seemed to be directly related to the extent of ATP breakdown. ADP and IMP thus showed a gradual increase during storage. The level of lactate in the muscles gradually increased during these storage times, also. On the other hand, the levels of those components in the muscle entirely recovered to their original levels within $3{\sim}6hrs$ storage after they were returned to conditions of recovery. The ratio of ATP to the ATP and its related compounds${ATP/(ATP+ADP+AMP+IMP){\times}100}$ in the muscle showed $45\%$ after 18hrs storage in cold-waterless conditions. Otherwise, ratios returned to their original levels within $3{\sim}6hrs$ of storage in recovery conditions.

• International Commerce and Information Review
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• v.16 no.4
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• pp.333-362
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• 2014

This study uncovered some issues that have constrained the development of logistics performance in Cambodia. Firstly, literature review was narrowed down to include studies involving the relationship between Logistics and transportation, the relationship between the infrastructure and economic growth and the important role of logistics on economic growth and poverty reduction. Then the next step the study identified some issues related to transport infrastructure that were assumed to cause the enhancement of logistics sector. Moreover, a case study about cost and time analysis was used to address some issues of logistics cost in Cambodia comparing to its neighboring countries: Thailand and Vietnam. Based on the time and cost analysis, it revealed that the logistics cost in Cambodia is much high than Thailand and Vietnam. In addition, some logistics issues were found through other two case studies about the export and trade facilitation. The participants raised some issues related to issuance of certificate of origin, the availability of information about agreements, laws, rules, and regulations, checkpoints along the corridor and opening hours of logistics service providers and slow processes. Then the authors suggested some appropriate solutions to answer to the current issues related to transport infrastructure and logistics sector in Cambodia.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.9 no.3
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• pp.132-139
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• 1997

CTD and current observation were taken to investigate the structure of the cold water in the Western Channel of the Korea Strait in October 1993. Thickness of the cold water in the deep trough of the strait changes from 20 m to 70 m according to the water depth. Thermocline between the Tsushima Warm Water and the cold water deepens from north to south with 0.00057 in slope. Temporal variation of the thickness appears to be related with the tidal current. The maximum variation is 20 m for 48 hours. Mean velocity of the cold water for 72 hours is 17 cm/sec southward. A simple model was used to understand dynamically the southward flow of the cold water and the return flow at the upper part in the lower layer. Calculated maximum southward flow and eddy viscosity coefficient are 7 cm/sec and 0.038 $m^2$/sec respectively in the model. Southward transport is $0.032$\times$10^6㎥/sec$ at the northern part in the trough and decreases from north to south due to the presence of the return flow. Southward transport increases with the increase in the upper layer transport but is not affected by the density of the upper layer or the interface slope.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.173-173
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• 1996

포도당 수송을 증가시키는 것으로 밝혀진 brazilin의 작용이 지방세포의 단백질과 결합하여 나타나는지 알아보기 위한 실험으로 brazilin의 단백결합 성질을 살펴보았다. Brazilin의 단백결합 실험에는 [$^3$H]-brazilin을 사용하여 결합된 양을 방사선 동위원소량으로 확인하였다. 먼저 일반적인 단백결합을 알아보기 위하여 산침전을 이용한 brazilin의 BSA에 대한 결합을 살펴보았다. 그 결과 brazilin은 시간의 경과에 따라 BSA에 결합하는 양이 증가하여 2시간에 최고치에 달했으며 그 양은 약 80% 정도였다. 세포내 단백과의 결합은 BSA에 대한 결합과는 달리 1시간만에 최고치에 달하였다. 세포내 분획에 분포하는 brazilin의 양을 알기 위하여 brazilin처리 후 세포내 분획을 원심분리로 얻어 brazilin의 양을 확인하였다. 그 결과 brazilin은 cytosol에 대부분 존재하며 microsomal membrane과 nuclear에 많은 양이 존재하는 것으로 확인되었다. SDS-PAGE를 이용하여 brazilin과 결합한 각 분획의 단백질을 살펴본 결과 brazilin은 cytosol의 30, 65KD, microsomal membrane의 45, 130KD, nucear의 26, 35KD, plasma membrane의 46, 228KD protein과 반응하는 것으로 확인되었다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.5
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• pp.758-763
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• 2003

Amino acid transporters play an important role in supplying nutrients to normal and cancer cells for cell proliferation. System L is a major transport system responsible for the N $a^{＋}$-independent, large neutral amino acids including several essential amino acids. L-type amino acid transporter 1 (LAT1), an isoform of system L amino acid transporter, is highly expressed presumably to support their continuous growth and proliferation in malignant tumors. 2-Aminobicyclo- (2,2,1) -heptane-2-carboxylic acid (BCH) is a model compound for study of amino acid transporter as a system L selective inhibitor. In the present study, we examined whether BCH induced growth inhibition in KB human oral squamous carcinoma cell line or not. The uptake of L-［$^{14}$ C］leucine by KB cells is inhibited by BCH in a concentration dependent manner with a Ι $C_{50}$ value of 75.3$\pm$6.2 ${\mu}{\textrm}{m}$ and a $K_{i}$ value of 98.7$\pm$ 4.1 ${\mu}{\textrm}{m}$. The growth of KB cells is inhibited by BCH in time dependent manner and concentration dependent manner with a Ι $C_{50}$ value of 11.1 $\pm$0.8 mM. In the DNA of KB cells treated with the various concentrations and various periods of BCH, the characteristic ladders associated with DNA fragmentation were not observed. These results suggest that BCH inhibits the growth of KB oral epidermoid carcinoma cells through the inhibition of transport of neutral amino acids into cells without DNA break down. This phenomenon will be a new rationale for anti-cancer therapy.y.