Choi, Ki Young;Jang, Eun Ji;Rhee, Han Cheol;Yeo, Kyung-Hwan;Choi, Eun Young;Kim, Il Seop;Lee, Yong-Beom
Journal of Bio-Environment Control
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v.24
no.3
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pp.243-251
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2015
This study aimed to determine the effects of root zone cooling using air duct on air temperature distribution and root zone and leaf temperatures of sweet pepper (Capsicum annum L. 'Veyron') grown on coir substrate hydroponic system in a greenhouse. When the air duct was laid at the passage adjacent the slab, the direction of air blowing was upstream at $45^{\circ}$. The cooling temperature was set at $20^{\circ}C$ for day and $18^{\circ}C$ for night. For cooing timing treatments, the cooling air was applied at all day (All-day), only night time (5 p.m. to 1 a.m.; Night), or no cooling (Control). The air temperature inside the greenhouse at a height of 40 and 80cm above the floor, and substrate and leaf temperatures, fruit characteristics, and fruit ratio were measured. Under the All-day treatment, the air temperature was decreased about $4.4{\sim}5.1^{\circ}C$ at the height of 40cm and $2.1{\sim}3.1^{\circ}C$ at the height of 80cm. Under the Night treatment, the air temperature was decreased about $3.4{\sim}3.8^{\circ}C$ at the height of 40cm and $2.2{\sim}2.7^{\circ}C$ at the height of 80cm. The daily average temperature in the substrate was in the order of the Control ($27.7^{\circ}C$) > Night ($24.1^{\circ}C$) > All-day ($22.8^{\circ}C$) treatment. Cooling the passage with either upstream blowing at $45^{\circ}$ or horizontal blowing at $180^{\circ}$ was effective in lowering the air temperature at a height of 50cm; however, no difference at a height of 100cm. Cooling the passage with perpendicular direction at $90^{\circ}$ was effective in lowering the air temperature at the height between 100 and 200cm above the floor; however, no effect on the temperature at the height of 50cm. A greater decrease in leaf temperature was found at 7 p.m. than that at 9. a.m. under both All-day and Night treatments. Fresh weight partitioning of fruit was in the order of the All-day (48.6%) > Night (45.6%) > Control (24.4%) treatment. A higher fruit production was observed under the All-day treatment, in which the accumulated average temperature was the lowest, and it may have been led to a higher proportion of photosynthate distributed to fruit than other treatments.
The emergence of antibiotic-resistant bacteria has been increased and become a public health concern worldwide. Many bacterial infections can be sequentially treated with different types of antibiotics. Thus, this study was designed to evaluate the changes in survival, antibiotic susceptibility, mutant frequency, ${\beta}$-lactamase activity, biofilm formation, and gene expression in Klebsiella pneumoniae after exposure to sequential antibiotic treatments of ciprofloxacin and meropenem. Treatments include control (CON; no addition), 1/2 MIC ciprofloxacin addition (1/2CIP), 2 MIC ciprofloxacin addition (2CIP), initial 1/2 MIC ciprofloxacin addition followed by 1/2 MIC meropenem (8 h-incubation) and 2 MIC ciprofloxacin (16 h-incubation) (1/2CIP-1/2MER-2CIP), initial 1/2 MIC ciprofloxacin addition followed by 1/2 MIC meropenem (8 h-incubation) and 2 MIC meropenem (16 h-incubation) (1/2CIP-1/2MER-2MER), and initial 1/2 MIC ciprofloxacin addition followed by 2 MIC ciprofloxacin(8 h-incubation) and 2 MIC meropenem(16 h-incubation) (1/2CIP-2CIP-2MER). No growth of K. pneumoniae was observed for the 2CIP throughout the incubation period. The numbers of planktonic cells varied with the treatments (7~10 log CFU/ml), while those of biofilm cells were not significantly different among treatments after 24-h incubation, showing approximately 7 log CFU/ml. Among the sequential treatments, the least mutant frequency was observed at the 1/2CIP-1/2MER-2CIP (14%). Compared to the CON, 1/2CIP-2CIP-2MER decreased the sensitivity of K. pneumoniae to piperacillin, cefotaxime, and nalidixic acid. The highest ${\beta}$-lactamase activity was 22 nmol/min/ml for 1/2CIP-1/2MER-2CIP, while the least ${\beta}$-lactamase activity was 6 nmol/min/ml for 1/2CIP-2CIP-2MER. The relative expression levels of multidrug efflux pump-related genes (acrA, acrB, and ramA) were increased more than 2-fold in K. pneumoniae exposed to 1/2CIP-1/2MER-2MER and 1/2CIP-2CIP-2MER. The results suggest that the sequential antibiotic treatments could change the antibiotic resistance profiles in K. pneumoniae.
This study was conducted to examine the effect of cool-season grass overseeding on the green period, turf quality and density in zoysiagrass lawn. Treatments were perennial ryegrass (PR) overseeding ($60g\;m^{-2}$) on medium-leaf type zoysiagrass, Kentucky bluegrass (KB) overseeding ($20g\;m^{-2}$) on medium-leaf type zoysiagrass and narrow-leaf type zoysiagrass, and no overseeding on medium-leaf type zoysiagrass. Overseeding of KB or PR effectively provided quality improvement of zoysiagrass lawn by extending green-period about one month in spring and two months in fall season. PR overseeding showed quick green cover within 2-3 weeks but decreased the quality of overseeded zoysiagrass lawn during the summer season. Whereas, KB overseeding showed slow green cover taking two to three month after seeding but provided stable and good turf quality throughout the years. KB or PR overseeding significantly increased the turf density of zoysiagrass lawn except the period of summer depression of PR. The ground coverage of cool-season grasses ranged from 30 to 80% with considerable seasonal variation. As a result, KB and PR have their strengths and weaknesses as an overseeding material. Thus, the use of KB and PR as a mixture would provide better overseeding performance in zoysiagrass lawn.
In order to develop the application method of compost manure (CM) and liquid manure (LM) for rice cultivation, experiments were conducted at silty loam paddy field in Gochang, Jeonbuk, a LM applied rate as N%; non-application, chemical fertilizer (CF) 100%, CM 50%+LM 50%, CM 30%+CF 70% and CM 30%+LM 70% as basal and additional fertilizer. $NH_4^+$-N content in paddy soil was higher with CF 100% application than the split application of compost and liquid pig manure fertilizer during the early stage of rice growth. However, there was no significant difference in the later part of rice growth. Amount of $NO_3^-$-N in leachate was decreased in CM 30%+LM 70% and CM 30%+CF 70% split applications compared to CF 100%. Amounts of OM and Avail $P_2O_5$, Exch. cations in soil of experiment after were highest with the split application of CM 50%+LM 50% and CM 30%+LM 70%. Amount of nutrient uptake of plants were no significant difference between the split application plots of CM and LM, but nitrogen utilization rate was 66% in average CM 50%+LM 50% and CM 30%+LM 70% to compared CF 100%. The rice yield of CM 50%+LM 50% was lower (90%) comparing that of CF 100% ($557kg\;10a^{-1}$). But the yield in CM 30%+CF 70% and CM 30%+LM 70% reached 96% in average, which did not show significant difference with that of CF 100%. Accordingly, LM 70% or CF 70% split application after CM 30% application was helpful in enhancing the physicochemical property of soil as well as reducing CF. It could be evaluated that this application in segmentation was better in productivity improvement and soil pollution reduction than the esinultaneous application of LM 100% in terms of split application in times of requirement for plants.
This study aimed to examine was conducted to the ability of microclimate control in old pine forests by surveying pine forest in Buddhist temples, where the pine forest are stably growing through active protection in the Gyeongnam region, and comparing variation characteristics of microclimate characteristics (temperature and humidity) and distribution of vegetation type. The study sites were pine forests protected well by Buddhist temples (Haein-sa, Beomeo-sa, Tongdo-sa, and Bulguk-sa) in the southeast region of Korea and thus known for stably growing young pine trees. According to the vegetation distribution status analysis, these pine forests did not have a high ratio of pine trees. Except for Tongdo-sa, the ratio of deciduous forest and mixed (deciduous and pine trees) forest had a much larger presence than that of pine forest. Measured data of microclimate showed that the Tongdo-sa area had significantly different characteristics compared to the other three areas. Tongdo-sa area showed a significantly higher diurnal range of temperatures and humidity than the other three areas, in both spring and summer. It is due to the difference in vegetation management. The forests around Tongdo-sa are mostly pine forests, except for the developed areas, while those in the other three areas have a dominant ratio of deciduous brad-leaved forests. Intensive control of pine forest is not effective in mitigating microclimate, i.e., temperature and air humidity. Stress caused by rising temperatures and decreasing air humidity is blamed for the decline of pine forests. Thus, the current active management of pine forests, such as the Tongdo-sa case, has been found to have a greater negative impact on the temperature and humidity stress. Therefore, we believe that a new change in forest management is necessary to increase the effect of mitigating the microclimate of pine forests.
Optimal environmental conditions, that sustained fastest growth, lowest mortality and abnormality of the scallop Patinopecten yessoensis, were identified from field experiments undertaken at Chumunjin during 1991-1998. Temperature within the water column 10~30 m depth ranged between 5 and 23$^{\circ}C$; high temperature and daily fluctuation resulted in growth retardation and heavy mortality of the scallop. Optimal salinity range was between 31.5 and 34.5%0 and water transparency 6.0 and 18.1 m, which was significantly affected by phytoplankton density. Chlorophyll concentration ranged between 0.04 and 3.51 f.lgfL. Low temperature and high chlorophyll concentration appear to support faster growth of the scallop. Optimal periods of transplantation for intermediate culture were between mid July and early November: cultured under high density during July-August as a first step and under low density during mid September through early November as a second step. Optimal stocking density in square net cage (<35${\times}$35 em) for intermediate culture was 30-40 individuals per cage for main culture using lantern net and 80 -100 individuals of the size of 1.5 ~ 3.0 em shell height per cage for sowing culture. During the intermediate culture, the highest growth was realized, when the cage was held at water depth between 10 and 15 m. Water depth below 25 m, however, was best to avoid mass mortality during the periods of abnormally high water temperature and high variation of water temperature. The daily growth rate during the intermediate culture was between 0.019~0.381 mm; low in January and February but high in March and April. It is suggested that the main culture is commenced before June under low stocking density to avoid the possibility of mass mortality during summer by high water temperature.
To determine suitable composition of nutrient solution according to soil textures in fertigation culture of cucumber using three strengths (S) of Yamasaki cucumber recipe, chemical changes of soil, growth characteristics and yield of cucumber were investigated. Electric conductivity of drainage solution was risen in all treatments, pH of loam soil was generally optimum level and that of sandy soil was high level. Photosynthetic rate in loam soil supplied the 1/2 S and transpiration rate in sandy soil supplied the 1.0 S were most low. Diffusive resistance in sandy soil supplied the 1.0 S was high. Chlorophyll contents was higher concentrations by the kinds of soil. Amount of drainage solution in sandy loam soil supplied the 1/2 S and loam soil supplied the 1.0 S were most much and little, respectively. Water absorption rate was the opposition to amount of drainage solution. Nutrient contents in soil except calcium were most high in the 1.0 S by the kinds of soil. Nutrient contents in leaves, nitrogen in sandy soil supplied 1.0 S, phosphorus in loam soil supplied 1/2 S, potassium in two soil supplied 1.0 S, calcium in loam supplied 1/2 S and sandy loam soil supplied 1.0 S, magnesium in loam soil supplied the 1/2 S was high. The growth and yield of fruit were more in loam than in sandy loam soil. Therefore, the suitable compositions of macro-nutrients for fertigation culture of cucumber were determined in loam and sandy loam soils as follows: In loam soil, they were $NO_3$-N 12.3, $NH_4$-N 1.0, P 3.0, K 5.9, Ca 5.7, Mg $3.5\;me{\cdot}L^{-1}$, whereas in sandy loam soil $NO_3$-N 11.7, $NH_4$-N 1.0, P 3.0, K 5.9, Ca 4.9, Mg $3.2\;me\;L^{-1}$.
Several parameters of hydraulic architecture relating to hydraulic conductance in xylem vessels were investigated in the current-year shoots of six species of deciduous oak trees. The above parameters were also investigated in the sprouts of Quercus mongolica and Q. variabilis, as well as in the seedlings of Q. mongolica and Q. acutissima. The values of specific conductivity, leaf specific conductivity and Hagen-Poiseuille's relative hydraulic conductivity relating to vessel diameter of Q. dentata were the highest in all of the species studied. The above values of most of the species studied were higher in May-June than in September-October because of increasing the vessel embolism by cavitation and so on through the growing season. The estimated values of relative hydraulic conductivity of vessel by Hagen-Poiseuille's empirical equation and the real values of hydraulic conductivity presented positive relationships in most of the species studied. Huber value and leaf specific conductity using leaf area or leaf weight generally exhibited similar patterns each other even if having some exceptions. The hydraulic conductances of sprouting shoots were much better than those of normal growing shoots in Q. rnongolica and Q. variabilis. The specific conductivity and leaf specific conductivity were rapidly decreased by the vessel embolism through cavitating just after cutting the shoots in Q. mongolica and Q. acutissima seedlings. Diurnal changes of the conductivities in the seedlings of Q. mongolica and Q. acutissima presented the possibility of their self-controlling of conductance by active moisture absorption under mild water stress. Specific conductivity and leaf specific conductivity, and so on of Q. acutissima seedlings subjected to periodical moisture stress or not have decreased through the growing season, but the influences of moisture stress to the conductance were not proved definitely because of influencing similarly and simultaneously to the development of xylem and leaf having inverse relation in the influences. The values of conductivities were higher generally in middle or upper parts of stems than root collar in the seedlings.
Park, Gab Soon;Chae, Soo Cheon;Oh, Chan Sik;Choi, Jong Myung
Horticultural Science & Technology
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v.33
no.4
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pp.511-517
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2015
This research was conducted to evaluate the influence of root restriction materials and media on the growth of runner plantlets of 'Seolhyang' strawberry in a nursery field. To achieve this, the influence of three kinds of root media on the growth of runner plantlets was monitored when polyethylene film was used as the root restriction material. In addition, the influence of various root restriction materials (RRS) such as transparent polyethylene film (PE), non-woven fabric (NF), perforated polyethylene film (PP), and root proofing sheet (RPS) on the changes in volumetric water content (VWC) and temperature of root media as well as growth of runner plantlet were investigated when expanded rice hull (ERH) was used as the root medium. In the comparison of root media, growth parameters such as leaf area and crown thickness at 20 d after fixation as well as crown thickness and fresh weights of root and above-ground tissue at 40 d after runner plantlet fixation were higher in the ERH treatment than in sandy loam and loamy sand. When the influence of RRS was compared, the VWC of ERH was 55% just after irrigation, but decreased to 26% at just before irrigation. Ranges of the VWC as influenced by irrigation cycle were 16 to 10% in the PP and less than 10% in the NF and RPS. The soil temperature in the PE treatment was around $1^{\circ}C$ lower than in NF, PP, and RPS. The differences between day and night temperatures were also smaller in the PE treatment rather than those in NF, PP, and RPS. The growths of runner plantlet 50 d after fixation showed that plant heights as well as fresh weights of root and above-ground tissue were higher in the PE treatment than in NF, PP, and RPS. NF and PP did not effectively restrict roots inside the medium and the roots of runner plantlets penetrated through the root restriction materials resulting in the formation of root system below the restriction materials. The above results indicate that ERH is more effective than sandy loam or loamy sand as root medium. PE rather than NF, PP, or RPS as root restriction material resulted in better growth of runner plantlets in propagation of 'Seolhyang' strawberry. The results of this research will be used for production of high quality runner plantlets in strawberry propagation.
Jun, Ha Joon;Byun, Mi Soon;Liu, Shi Sheng;Jeon, Eui Hwan;Lee, Yong Beom
Horticultural Science & Technology
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v.31
no.2
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pp.173-178
/
2013
Experiments were conducted to investigate the optimum concentration of nutrient solution for strawberry 'Maehyang' bred domestically for exportation in hydroponics. Nutrient solutions for strawberry, which was made by Yamazaki, were supplied electrical conductivity (EC) 0.6, 0.8, 1.2, and $1.8dS{\cdot}m^{-1}$ after planting on cocopeat medium during the experiment period. Growth of shoot of strawberries did not show any statistical differences among treatments. Fruit length showed the longest in EC 0.8 and $1.2dS{\cdot}m^{-1}$, followed by 0.6 and $1.8dS{\cdot}m^{-1}$ in the first and third cluster. It showed the shortest in EC $1.8dS{\cdot}m^{-1}$ in the second cluster but there were no significant differences among treatments in the fourth cluster. Fruit diameter did not show significant differences among treatments in the first and second cluster but was the longest in the lowest concentration EC $0.6dS{\cdot}m^{-1}$ in the third cluster. The shortest was in EC $1.8dS{\cdot}m^{-1}$ in the fourth cluster. The heaviest mean fruit weight appeared in EC 0.8 and $1.2dS{\cdot}m^{-1}$, and the lightest was in EC $1.8dS{\cdot}m^{-1}$ in the first cluster and also lightest in EC $1.8dS{\cdot}m^{-1}$ but no significant differences was found among other treatments in the second & third cluster. Also the fruit weight was significantly light in plants grown in EC $1.8dS{\cdot}m^{-1}$ than $0.6dS{\cdot}m^{-1}$ in the fourth cluster. Soluble solids content of fruit was the highest in EC $0.6dS{\cdot}m^{-1}$ in all cluster. As a result, we came to the conclusion that the optimum EC for strawberry 'Maehyang' was EC 0.8 - $1.2dS{\cdot}m^{-1}$ during low temperature season. This result will be utilized as an indicator for strawberry hydroponics.
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