• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.3
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• pp.224-232
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• 2017

In this study, we investigated the disease, insect damage, growth, and yield characteristics of sorghum and foxtail millet in an organic paddy-upland rotation system at Anseong-si Gyeonggi province, Korea, in 2016. Seven varieties of sorghum and foxtail millet are used in this study. A rotational upland field and continuously cropped upland field were compared in order to establish an optimum cultural method and to select the best sorghum and foxtail varieties for a paddy-upland rotation system. The incidence rate of major diseases was higher for sorghum than for foxtail millet. Moktaksusu (22.8%) had the highest incidence rate among sorghum varieties. Hwanggeumchal (7.0%) had the highest incidence rate among foxtail millet varieties. DS202 (89.3%) was the most severely damaged by Ostrinia furnacalis larva, which is the main pest of sorghum and maize. The weed numbers, and the weed fresh and dry weights were lower in the rotational upland field than in the continuously cropped upland field. Futhermore, the number of weed plants decreased by 42% and the dry weight of weeds decreased by 33% in the rotational upland field compared to the continuously cropped upland field. The culm length of sorghum and foxtail millet were 7.9 cm, and 3.4 cm longer, respectively, in the rotational upland field than in the continuously cropped upland field. The average yield of sorghum per 10 a increased by 90% in the rotational upland field compared to continuously cropped upland field. However, there was no significant difference between the rotational upland field and the continuously cropped upland field for foxtail millet. The most suitable varieties for a paddy-upland rotation system were judged to be Hwanggeumchal, Donganmae, and DS202 for sorghum, and Samdachal, Samdamae, Dahwangmae for foxtail millet.

• Microbiology and Biotechnology Letters
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• v.26 no.2
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• pp.122-129
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• 1998

It were reported that antifungal mechanism of Enterobacter cloacae is a volatile ammonia that produced by the strain in soil, and the production of ammonia is related to the bacterial urease activity. A powerful bacterium SH14 against soil-borne pathogen Fusarium solani, which cause root rot of many important crops, was selected from a ginseng pathogen suppressive soil. The strain SH14 was identified as Bacillus subtilis by cultural, biochemical, morphological method, and $API^{circledR}$ test. From several in vitro tests, the antifungal substance that is produced from B. subtilis SH14 was revealed as heat-stable and low-molecular weight antibiotic substance. In order to construct the multifunctional biocontrol agent, the urease gene of Bacillus pasteurii which can produce pathogenes-suppressive ammonia transferred into antifungal bacterium. First, a partial BamH I digestion fragment of plasmid pBU11 containing the alkalophilic B. pasteurii l1859 urease gene was inserted into the BamH I site of pEB203 and expressed in Escherichia coli JM109. The recombinant plasmid was designated as pGU366. The plasmid pGU366 containing urease gene was introduced into the B. subtilis SH14 with PEG-induced protoplast transformation (PIP) method. The urease gene was very stably expressed in the transformant of B. subtilis SH14. Also, the optimal conditions for transformation were established and the highest transformation frequency was obtained by treatment of lysozyme for 90 min, and then addition of 1.5 ${mu}g$/ml DNA and 40% PEG4000. From the in vitro antifungal test against F. solani, antifungal activity of B. subtilis SH14(pGu366) containing urease gene was much higher than that of the host strain. Genetical development of B. subtilis SH14 by transfer of urease gene can be responsible for enhanced biocontrol efficacy with its antibiotic action.

• Microbiology and Biotechnology Letters
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• v.38 no.3
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• pp.295-301
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• 2010

Sheath rot and dry rot disease caused by Pseudomonas marginalis and Fusarium oxysporum were serious problems in garlic farmland. In this study, total of 160 indigenous antagonistic bacteria were isolated from 16 farmlands in Yeongcheon, Korea. Among these, 15 strains were able to inhibited P. marginalis and F. oxysporum. The 16s rDNA genes of the selected 15 strains were amplified and sequenced. The strains has strong antagonistic ability against garlic pathogens was achieved Bacillus subtilis YC82, B. vallismortis YC84, B. amyloliquefaciens YC240. The selected 3 strains tested for investigation of antifungal mechanisms further analyses; 3 strains of these validated for production of siderophore, ${\beta}$-glucanase and chitinase using CAS (chrome azurol S) blue agar, CMC-congo red agar and DNS method. The 3 strains were able to utilized insoluble phosphate as dertermined by vanado-molybdate method. The 3 strains verified for production of auxin and gibberellic acid using Salkowski test and holdbrook test. Also, 3 strains showed stimulation germination, stem growth promoting activity on the in vivo test. The 3 strains were able to effectively suppress P. marginalis and F. oxysporum causing sheath rot and dry rot diseases on the in vivo pot test.

• Korean journal of applied entomology
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• v.16 no.3 s.32
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• pp.163-170
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• 1977

Potato diseases, especially mosaics and leaf roll, appear to reduce potato yield in Korea more than any other factor. A seed potato certification program was established at the Alpine Experiment Station (AES) in 1961 to produce high quality seed potatoes for distribution to Korean farmers. The present program for production of certified seed of Namjak (Irish Cobbler), the only variety recommended for spring plantings, is outlined. In 1976, approximately 10,000 MT of certified grade Namjak seed was produced by members of two Seed Producers Cooperatives in the Daekwanryeong area for distribution by the Office of Seed Production and Distribution (OSPD). The seed was inspected and certified by officers of the National Agricultural Products Inspection Office (NAPIO). Although the quality of the certified seed is far superior to that used by many farmers, the supply planted less than 1/5 of the 1977 potato crop. Certified seed of Shimabara, the variety recommended for autumn plantings, is not produced in Korea. The yield response of virus infected seed to improved cultural practices is poor. Therefore, an increase in potato acreage and yields appears to be possible only if more good quality seed is used by Korean farmers. A two or three fold increase in seed supply would be desirable. The volume of seed could be increased by expanding the production area and by improving yield in seed fields. More land is available in the alpine area and good seed potatoes could be grown in other parts of Korea. Planting better quality seeds and using better cultural pracitces would improve seed yields. Several techniques could be used to improve the quality of elite seed produced at AES. Changes in seed potato certification program should be made so that healthy seed stocks of new varieties can be released rapidly.

• Journal of Korean Society of Forest Science
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• v.102 no.2
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• pp.272-280
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• 2013

This study aims to develop a standard diagnostic table for management and administration that oak mushroom cultivators of forestry households can utilize. By diagnosing their current level of management with the table, the cultivators themselves will be able to grasp and address their operational challenges better. The table is composed of; questions on the status of forestry households; columns for a series of management performance indices, and; questionnaire with 4 categories and 20 subcategories to check the current level of management and administration by the households. In order to prepare the standard diagnostic table for oak mushroom management, 196 forestry households throughout 10 areas - Cheong-yang, Gong-ju, Bu-yeo and Seocheon in Chungcheongnam-do, Mun-kyung and Ye-cheon in Gyeongsangbuk-do, Jin-an in Jeollabuk-do, Jangheung and Jang-seong in Jeollanam-do, and Jeju Islands - were interviewed with and a total of 190 questionnaires were acquired and made use of in the result analysis. Then, the score on management level of each forestry household was determined in the way of aggregating scores from each subcategory. The overall average score of every household was calculated at 62.2 point with more than half of the respondents, 54.7%, belonging to the range of 60 to 80. When considered by regional groups, the average score of Jin-an was the lowest with 57.6 point while that of Jang-seong was the highest with 69.6 point. In case of the 'cultivation management' category, there were a lot of cultivators who expressed a negative awareness of the term 'pest control' because they had a tendency to think the term in connection with 'herbicides or pesticides'. So it is inevitable to adapt and modify existing groups and grades to make sure that the cultivators can make a right choice without confusing the concept 'pest control' with 'herbicides or pesticides'. Meanwhile, the average scores of 'management and administration' categories were mostly low. It was remarkable in these categories that forestry households in Jeolla province, which had remained in lower ranks in the other three categories, recorded higher scores than those in Chungcheong province, boasting a relatively high level of management and administration.

• Journal of Korean Society of Forest Science
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• v.109 no.3
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• pp.259-270
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• 2020

Extreme weather events, such as heat and drought, have occurred frequently over the past two decades. This has led to continuous reports of cases of forest damage due to physiological stress, not pest damage. In 2014, pine trees were collectively damaged in the forest genetic resources reserve of Sogwang-ri, Uljin, South Korea. An investigation was launched to determine the causes of the dieback, so that a forest management plan could be prepared to deal with the current dieback, and to prevent future damage. This study aimedto 1) understand the topographic and structural characteristics of the area which experienced pine tree dieback, 2) identify the main causes of the dieback, and 3) predict future risk areas through the use of machine-learning techniques. A model for identifying risk areas was developed using 14 explanatory variables, including location, elevation, slope, and age class. When three machine-learning techniques-Decision Tree, Random Forest (RF), and Support Vector Machine (SVM) were applied to the model, RF and SVM showed higher predictability scores, with accuracies over 93%. Our analysis of the variable set showed that the topographical areas most vulnerable to pine dieback were those with high altitudes, high daily solar radiation, and limited water availability. We also found that, when it came to forest stand characteristics, pine trees with high vertical stand densities (5-15 m high) and higher age classes experienced a higher risk of dieback. The RF and SVM models predicted that 9.5% or 115 ha of the Geumgang Pine Forest are at high risk for pine dieback. Our study suggests the need for further investigation into the vulnerable areas of the Geumgang Pine Forest, and also for climate change adaptive forest management steps to protect those areas which remain undamaged.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.61 no.2
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• pp.79-86
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• 2016

The late-maturing black rice cultivar Shinnongheugchal from Jeollabuk-do Agricultural Research and Extension Service was used as the plant material for estimating growth characters, quality and yield from the vegetation period to harvest age. This study was performed to select an optimum combination of nitrogen level and planting density for the maximum yield of Shinnongheugchal. The plant height, number of tillers, and SPAD index were higher when the combination of 70 hills per $3.3m^2$ and 13 kg/10 a nitrogen level was used at 30 days after transplanting. The heading date for the combination of 70 hills per $3.3m^2$ and 15 kg/10 a nitrogen level, and 80 hills per $3.3m^2$ and 15 kg/10 a nitrogen level was August 22. The heading date for the other combinations was August 21. The combination of 70 hills per $3.3m^2$ and 13 kg/10 a nitrogen level yielded the highest number of tillers at 40 days after flowering. Even though the lodging index was increased with increasing nitrogen levels, field lodging did not occur until harvest time. Seed nitrogen concentration in the combination of 70 hills per $3.3m^2$ and 13 kg/10 a nitrogen level showed a significant difference when compared with the other combinations. The black rice yield varied significantly, and the highest yield was observed in the combination of 70 hills per $3.3m^2$ and 13 kg/10 a nitrogen level. The yield was significantly correlated with seed nitrogen concentration. The maximum yield was estimated to be 14.67 kg/10 a nitrogen level by using the regression equation. On average, the coloring degree of the black rice was higher at planting density of 70 hills per $3.3m^2$ than at 80 hills per $3.3m^2$. The highest yield of perfect black rice was obtained using the combination of 70 hills per $3.3m^2$ and 13 kg/10 a nitrogen level. Our findings demonstrate that a nitrogen level of 13-14 kg/10 a can be used to obtain the maximum yield from Shinnongheugchal with yield, cyanidin 3-glucoside content, and perfect black rice yield as the standard.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.317-324
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• 2017

The purpose of this study was to provide basic data on the genetic and environmental effects of stickiness in glutinous rice varieties. In our study, we analyzed the genotype-by-environment ($G{\times}E$) interactions of the stickiness using six glutinous rice varieties under six environmental conditions. AMMI (Additive Main Effects and Multiplicative Interaction) analysis results showed that genotype (variety, G), environment (cultivation region, E) and $G{\times}E$ interaction were highly significant (P < 0.001). Among all the variations of stickiness for glutinous rice varieties, the environmental effect was 24.5%, the genetic effect was 37.1%, and the $G{\times}E$ interaction effect was 28.9%. From the AMMI analysis, the IPCA1 scores of Aranghangchal (G6, IPCA1: 3.85) and Hwaseonchal (G4, IPCA1: -5.24) was lower than other varieties. On the other hand, the Sangjuchal (G1, IPCA1: -61.23) and Boseogchal (G2, IPCA1: 41.21) were highly affected by environmental effects. In this study, there were large differences in stickiness according to region of cultivation. In the future, it is considered that a precise study should be carried out on the environmental factors that may increase the stickiness of glutinous rice varieties.

• The Korean Journal of Pesticide Science
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• v.15 no.1
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• pp.55-60
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• 2011

To work out quality control methods of environmental-friendly organic materials (EFOMs), the reason and basis for EFOM-selection and farmer's favorite formulation type of EFOMs, etc were investigated on farmers who had been practicing environmental-friendly agriculture. EFOMs used were soil amendments, control agents of plant diseases and insect pests, plant growth promotion formulations, in turns. In EFOMs application time, 22.7% of farmers sprayed EFOMs without delay after they were bought, in other hand, 77.3% of farmers used EFOMs which had been bought and stored for some period. Microbial density on seventeen environmental-friendly microbial formulates (EFMFs) including microbial pesticides, a microbial fertilizer, and environmental-friendly organic materials was investigated at different storing temperature and shelf life. When the microbial density of EFMFs was investigated without delay after they were bought, all used microbial pesticides and a microbial fertilizer was confirmed to be optimal for the certified density but two of environmental-friendly organic materials was confirmed not to be optimal. When microbial density of 17 EFMFs were investigated after storing them for six months at $4^{\circ}C$, only one of 9 microbial pesticides was confirmed not to be optimal, the other hand four of seven environmental-friendly organic materials not to be optimal, which each of their microbial density was less than the certified density. Population dynamics of microbial agents was much more influenced in fluctuated temperature (room temperature) than in static temperature condition ($5^{\circ}C$ and $25^{\circ}C$). Shelf life of microbial agents according to microbial formulation type were high in granule type, liquid wettable type and liquid type in turns.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.4
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• pp.76-83
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• 2018

The purpose of this study is to analyze people who applied for and passed engineer landscape architecture examination that had been conducted from 2007 to 2016, the test frequency and trends by the question types in the landscape structure construction and management area, and the test tendencies and features by question types, and thereby to find the test trends for landscape structure construction and management in engineer landscape architecture examination. the analysis results are presented as follows: The people who applied for and passed engineer landscape architecture examinations that had been conducted from 2007 to 2016 were analyzed. as a result, the numbers of applicants and those who passed the examination have been on the decrease from 2011 and from 2012, respectively. the 10-year average rate of successful applicants for engineer landscape architecture examination was 11.2%. The test frequency and trends by the question types in the landscape structure construction area, and the test tendencies and features were analyzed. as a result, based on the key words in the seven categories (construction plan & process management, landscape materials, landscape planting foundation, work classification based construction, landscape estimation, basic structural mechanics, and survey), the questions about work classification based construction accounted for the largest, or 25.2%, and the questions about landscape planting foundation accounted for 3.3%. therefore, landscape planting foundation had lower test frequency and was less important than other categories. The test frequency and trends by the question types in the landscape management area, and the test tendencies and features were analyzed. as a result, based on the key words in the nine categories (operation and use & maintenance, pruning management, fertilization management, weed management, irrigation and drainage management, wintering management, pest management, and lawn management, and landscape facility management), the questions about operation and use & maintenance accounted for the largest, or 37.2%, and the numbers of the questions about fertilization management and irrigation & drainage management and of the questions about waterscape facility of landscape facility management have been on the increase from 2011 and from 2015, respectively. According to the analysis on the test tendencies for landscape structure construction and management areas in the examination there have been questions in a wide range and variety of categories. in terms of the landscape structure construction area, the frequency of questions in work classification based construction, landscape materials, and excellent quality in terms of the landscape management area, the frequency of questions in fertilization management, irrigation & drainage management, and waterscape facility of landscape facility management tends to increase because of environmental factors like climate change.