• Journal of radiological science and technology
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• v.2 no.1
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• pp.3-9
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• 1979

이상 방사면역측정(放射免疫測定)의 기본원리(基本原理), 순수(純粹)한 항원(抗原), 표지항원(標識抗原), 항체생성(抗體生成), 표준곡선(表準曲線)의 작성(作成), 항원항체복합체(抗原抗體複合體)의 분리방법(分離方法)에 대(對)하여 설명(說明)하였다. 생물학적방법(生物學的方法),화학적방법(化學的方法), 기계적(機械的)인 측정방법(測定方法)에 의(依)해 생체내(生體內)의 미량(微量)으로 존재(存在)하고 있는 물질(物質)들을 정확(正確)하게 측정(測定)할 수 없다. 그러나 방사면역측정법(放射免疫測定法)에 의(依)해 쉽게 측정(測定)되어 기관(器管)의 기능검사(機能檢査)는 물론(勿論) 각(各) 기관(器管)과의 상관관계(相關關係)를 분석(分析)하여 질병(疾病)의 진단(診斷), 치료경과(治療經過)을 평가(評價)하는데 매우 중요(重要)한 정보(情報)를 제공(提供)해주고 있다. 이러한 점(點)을 고려(考慮)하여 이 방법(方法)을 잘 습득(習得)하고 진료(診療)의 수단(手段)으로 널리 이용(利用)되어 의료기술발전(醫療技術發展)에 토대(土臺)가 되기를 바란다.

• Journal of radiological science and technology
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• v.7 no.1
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• pp.93-98
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• 1984

• Development and Reproduction
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• v.9 no.2
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• pp.65-83
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• 2005

The three important phases in the development of ligand immunoassays are identified and summarized. The competitive radiolabelled hormone measurement had been developed in the first and early in the second generations(1950s to 1960s), such as radioimmunoassays(RIA) or immunoradiometric(saturation) assays(IRMA), and used in all most of the hormone and also analyte in biological samples. In the second generation, ultrasensitive non-isotopic immunoassays(NIA) were developed using monoclonal antibodies(McAb), labelling the McAb and high specific activity non-isotopic labels. After their usefulness, advantages and disadvantages has been evaluated and non-competitive methods are discussed. The chip/microarray based multianalyte ligand assays(microspot or genechip methods) are developed and known as alternative ones in the third generation. We summarize the developments of NIAs and its usefulness, and then introduce briefly the new ligand assays.

• Journal of radiological science and technology
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• v.10 no.1
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• pp.85-90
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• 1987

Preliminary studies on the measurement of the free thyroxine in the serum with Amerlex $FT_{4}$ RIA kit were investigated using a tracer as $^{125}I-T_{4}$ derivative which is not almostly bound to thyroxine binding globulin, etc. The results are followed: 1. Linearity was tested on standards at various concentrations, and reproducibility and accuracy was excellent. 2. The antibody specificity is also excellent, and standard calibration curve of total $T_{4}$ was similar that of adding the TBG inhibitor. 3. Each value of $T_{4}$ in serum (the normal group, the hypothyroidism patients the pregnant women and the TBG dificiency patients) was not significant. As mentioned above, this method is more simple and rapid, compared to the other method.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.6
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• pp.1030-1034
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• 2000

The ovalbumin, a most sensitive egg white protein to irradiation was purified from irradiated hen's eggs. Eggs were irradiated in their shells to 0~7 kGy. To investigate for a practical use in identifying of irradiated eggs, competitive ELISA using ovalbumin was peformed. The binding activity of ovalbumin to anti-ovalbumin IgG was reduced in a dose-dependent manner by irradiating up to 7 kGy, and consider-ably lowered after irradiating at 7 kGy. The concentration of 50% inhibition of ovalbumin to IgG was increased to 1.5~3.7 times in an irradiation dose-dependent relationship. SDS-PAGE of ovalbumin showed that the partial breakdown of ovalbumin was induced by irradiation. The lowering of binding activity was probably due to the partial breakdown of ovalbumin by irradiation. These results demonstrated that the ELISA should be quite useful and effective methods for the identification of irradiated eggs.

• Journal of Radiation Protection and Research
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• v.22 no.2
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• pp.103-109
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• 1997

Adult male rats were exposed to a whole body with a single dose of 1.0, 2.0, 3.0, 5.0, and 7.0 Gy. The animals were sacrificed 48, 72, 96 and 216 hours following exposure. A competitive enzyme linked immunosorbent assay(ELISA) with antigen immobilized on the solid phase has been developed to measure ceruloplasmin in rat serum and complete dose response curves. Ceruloplasmin was purified from the plasma of turpentine treated male rats. Coating of ceruloplasmin had more effectiveness in 10 mM Tris-HCI, 150 mM sodium chloride, pH 7.4 than in 50 mM carbonate/bicarbonate buffer, pH 9.6. The coating range for ceruloplasmin was $70{\sim}140ng$/well. Levels of ceruloplasmin increased to maximum on the $72{\sim}96$ hours after irradiation. Slope of between response and dose was greatest value 96 hours following irradiation. Normal ceruloplasmin levels were not recorded 216 hours following exposure. In 0.1 Gy irradiated group, levels of ceruloplasmin also increased to maximum on the $72{\sim}96$ hours following irradiation. The concentration of this protein remained significantly different from control value, 196 hours after exposure. Ceruloplasmin was identified as one of the major acute phase protein following irradiation and further studies about gene expression and regulation would be necessary for radiation protection.

• Journal of radiological science and technology
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• v.11 no.1
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• pp.25-31
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• 1988

Studies on the clinical significance with Amerlex $FT_{4}$ RIA kit observing the determination of $FT_{4}$ were investigated using a tracer as $^{125}I-T_{4}$ derivative which is not almostly bound to thyroxine binding globulin, etc. The results are followed; 1. $FT_{4}$ value($1,55{\pm}0.38ng/100ml$) of normal group was not accorded that of hyperthyroidism with Amerlex $FT_{4}$ RIA kit, and was higher than that of hypothyroidism. 2. $FT_{4}$ value was lower level in chronic-kidney disfunction syndrome whereas, it was normal in a cancer patient, a woman in pregnancy and a patient in TBG disfunction. 3. The value of this method is a good corelationship at that of equilibrium dialysis method. (r=0.931) 4. $FT_{4}$ value by this kit was linear relationship to those of the other kit (Gamma Coat and Liquisol), and the normal value of each methods was also similar. As mentioned above, this method is more simple and rapid, compared to the other method. Therefore, it was thought that this method is a very useful clinically.

• Parasites, Hosts and Diseases
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• v.24 no.1
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• pp.25-41
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• 1986

The applicability of micro-ELISA was evaluated in human neuro-cysticercosis using paired samples of serum and CSF. A total of 355 cases who were mostly neurologic patients was subjected. Cystic fluid of C. cellulosae was used as antigen in protein concentration of $2.5{\;}{\mu}g/ml$. Serum was diluted to 1 : 100 and CSF was undiluted in the assay for the specific IgG antibody level. The differential criterion of the positive reaction was the abs. of o. 18 in both samples. The results were summarized as follows: 1. The overall sensitivity of the micro-ELISA in 71 confirmed neurocysticercosis was 90.1% ; the sensitivity by serum was 77.5% and that by CSF was 83.1%. CSF was a more sensitive and valuable material. Most of the false negative cases of neuro-cysticercosis showed far lower level of abs. rather than marginal. 2. The overall specificity of the micro-ELISA in 52 confirmed other neurologic diseases was 88.5%; the specificities by serum and by CSF were 94.2% respectively. Cases of other neurologic diseases did not show false positive reactions in both samples. 3. When serum was assayed, taeniasis(2/18), sparganosis(2/20), paragonimiasis(1/56), clonorchiasis(1/15) and fascioliasis(1/1) cases showed cross reactions. When CSF was assayed, 2 ot 10 neuro-sparganosis showed cross reactions while none of 9 neuro-paragonimiasis showed it. Out of 71 confirmed neuro-cysticercosis cases, 6 and 11 showed cross reactions by serum and CSF to crude extract antigen of sparganum; but no case did show it to crude extract antigen of Paragonimus westermani. 4. Ventricular CSF showed low or negative levels of IgG antibody than lumbar CSF unless the lesion was at the lateral ventricle itself. 5. Out of 4 racemose cysticercosis cases, 3 showed positive reaction in serum while all of 3 examined CSF were positive. The above results indicated that the serological test for detecting the specific IgG antibody by micro-ELISA using paired samples of serum and CSF was very helpful for clinical differentiation of neuro-cysticercosis from neurologic diseases of other causes.

• Korean journal of applied entomology
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• v.31 no.4
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• pp.371-378
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• 1992

The ecdysteroid titers of representive developmental stages of the blackblow fly, Phormia regina, were determined by radioimmunoassay and the effect of ecdysteroid on the oocyte maturation was investigated. Prior to every molts ecdysteroid levels began to increase sharply, suggesting ecdysteroid was the major component for egg-larval, larval-larval, and larval-pupal transformation. A difference in the levels of ecdysteroid between male and female was ob¬served during adult life span. Following the protein meal, ecdysteroid in the females increased rapidly to a maximum at 96 hr of age when terminal oocyte fully matured. Effect of ecdysteroid on oocyte development was determined for control and ecdysone-treated female flies after the liver-feeding. The growth of oocyte in the flies treated by $\mu$g of ecdysone, along with the control flies, was not facilitated. When the flies treated by 5 $\mu$g of ecdysone, however, duration of oocyte maturation was shorter than those of other two groups. This can be suggested that oocyte development in P. regina is due to the critical level of ecdysone.

• Parasites, Hosts and Diseases
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• v.33 no.4
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• pp.395-398
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• 1995

A 56 year-old Korean housewife/farmerlgoat keeper suffered from right upper quadrant pain and fever with chills. In the abdominal sonogram and computerized tomography, multiple, 2-3 cm, irregular shaped cavities were observed in the right lobe of liver. A liver biopsy revealed extensive central necrosis with Characot-Leyden crystals surrounded by palisading histiocytes, eosinophil-rich inflammatory infiltration. Worm was not observed. However, the serologic test for Fusciola-specific IgG antibody by micro-ELISA was positive. Prior antibody levels did not differ and eosionophilia persisted 6 and 16 months after praziquantel treatment although the cavitaxy lesions in the liver disappeared 6 months after the treatment. Reported herein is a human case of invasive fascioliasis diagnosed clinically by a combination of radiological, histopathological and serological studies.