• The Korean Journal of Pharmacology
• /
• v.27 no.2
• /
• pp.135-144
• /
• 1991

Using T-lymphocytes obtained from rat peripheral blood, we found that the 44kD/pI6.8 protein was the major phosphoprotein of T-lymphocytes under basal condition, and that the 44kD/pI6.3 protein was a new phosphoprotein appeared in T-lymphocytes stimulated with ${\beta}-agonist$. The phosphorylation of the 44kD/pI6.3 protein was also induced by forskolin but inhibited by H-8 pretreatment. To clarify the character of the 44kD/pI6.3 protein, we used Con-A and kinase inhibitors, H-7 and W-7. Con-A stimulation induced phosphorylation of 44kD/pI 6.3 protein but that was inhibited by W-7 pretreatment. The phosphorytation of 44kD/pI6.3 protein was not induced by the PKC activator, PMA. Instead, the phosphorylation of 44kD/pI6.8 protein was reduced by H-7, a PKC inhibitor. From the above results,it can be concluded that the 44kD/pI6.3 protein can be a common substrate for A-kinase and CaM kinase. The two dimensional tryptic peptide mapping revealed that the 44kD/pI6.8 and 44kD/pI6.3 proteins are different.

• Journal of Korean Society of Steel Construction
• /
• v.13 no.5
• /
• pp.567-575
• /
• 2001

In order to consider statistical properties of probability variables used in the structural analysis the conventional approach using the safety factor based on past experience usually estimated the safety of a structure Also the real structures could only be analyzed with the error in estimation of loads material characters and the dimensions of the members. But the errors should be considered systematically in the structural analysis Safety of structure could not precisely be appraised by the traditional structural design concept Recently new aproach based on the probability concept has been applied to the assessment of structural safety using the reliability concept Thus the computer program by the Probabilitstic FEM is developed by incorporating the probabilistic concept into the conventional FEM method. This paper estimated for the reliability of a plane stress structure by Advanced First-Order Second Moment method using von Mises, Tresca and Mohr-Coulomb failure criterions. Verification of the reliability index and failure probability of attained by the Monte Carlo Simulation method with the von Mises criterion were same as PFEM, but the Monte Carlo Simulation were very time-consuming. The variance of member thickness and load could influence the reliability and failure probability most sensitively among the design variables from the results of the parameter analysis. The proper failure criterion according to characteristic of materials must be used for safe design.

• The Journal of Fisheries Business Administration
• /
• v.36 no.2 s.68
• /
• pp.1-24
• /
• 2005

The purpose of the study are summarized as follows : First, it has researched the possibility of the Distribution Information application in Sea Food Wholesale Market. Second, the effects which the Wholesale Market Company and the Intermediary Wholesaler Corporations can obtain in Sea Food Wholesale Market by building up a Distribution Information are classified into the effects of task, cost, and competition. It has analyzed the influence of these effects in direct and indirect Trust between the Wholesale Market Company and the Intermediary Wholesaler. Third, it has recognized the upcoming problems in Sea Food Wholesale Market by building up a Distribution Information, and it has suggested a plan to make the Distribution Information application successful in Sea Food Wholesale Market. This study has used a questionnaire to verify 5 hypotheses. Research model, factor analysis, correlation relationship analysis. The result of this study are summarized as follows : Building up the Distribution Information influences positively on the effectiveness of task, cost and competitiveness regardless of it being the Wholesale Market Company or the Intermediary Wholesaler corporation. However, the results of this analysis are to verify differences according to the degree of construction of the Distribution Information and the degree of the perception of the problems between the Wholesale Market Company and the Intermediary Wholesaler corporations have shown that there were distinct differences in the degree of computerization and of efforts to build a Distribution Information. Also there were distinct differences according to the degree of perceiving problems relating to building up the Distribution Information and the stages of the Distribution Information between the Wholesale Market Company and the Intermediary Wholesaler corporations. This study suggest three important steps that will help to establish a successful Distribution Information. First, the Wholesale Market Company and the Intermediary Wholesaler corporations should make efforts to increase mutual profits in partnership, and make direct Trust by sharing mutual information. Second, the lack of understanding of the Distribution Information between departments within the company requires educating employees about the Distribution Information. It is necessary to expand the communication networks of the Distribution Information between the Wholesale Market Company and the Intermediary Wholesaler. Third, mutual exchange of Information should be possible to offer systematic exchange of Information between the Wholesale Market Company and the Intermediary Wholesaler corporations.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.9
• /
• pp.1415-1422
• /
• 2014

Hot water extraction concentrate was prepared from Alliun hookeri root (AHR) to evaluate its applicability to yogurt. The highest antioxidant activity of hot water concentrates was obtained under extraction conditions of 4 hr at $95^{\circ}C$. Antioxidant activities measured by DPPH radical assay, ABTS radical cation assay, reducing power, and cheating activity were highly correlated with total phenolic (89.51 mg/g) and total flavonoid (52.71 mg/g) contents, with R values of 0.94 and 0.96, respectively. Yogurt was fermented with a commercial lactic acid bacteria mixed strain (Yo-mix$^{TM}$ 305) for 10 hr at $42^{\circ}C$ after addition of 0~10% (w/w) hot water concentrates from AHR to yogurt base. As fermentation proceeded, pH and $^{\circ}Brix$ of yogurt decreased from 6.57~6.60 to 4.34~4.51 and from 8.10~8.90% to 4.60~5.25%, respectively, whereas titrate acidity, viscosity, and viable cell numbers increased from 0.22~0.23% to 1.01~1.10%, from $0mPa{\cdot}s$ to $202.55{\sim}290.50mPa{\cdot}s$, and from 6.40~6.80 log CFU/mL to 8.60~9.20 log CFU/mL, respectively. There was no significant difference in any sensory attribute between the control and 2.5% addition group, suggesting that 2.5% hot water concentrate from AHR could be used to manufacture yogurt.

• Journal of the Korean Data and Information Science Society
• /
• v.22 no.3
• /
• pp.515-521
• /
• 2011

There are increasing demands for the producing and breeding new domestic riding horses for the vitalizations of horse riding industry in Korea, according as 'Horse Industry Support Act' became. In this study, we were to develop the functional relation through the conformation comparison & body composition analysis. 76 heads of 5 breeds utilized for riding horses in Korea were used and their body measurements on 12 items were measured and cluster analysis was conducted to determine the correlation relation among them. The measurements were standardized that (height, croup height, pelvis length), and (hip width, width of pelvis) were highly correlated. In these results of the decision tree, we confirmed to classify the breed type determination by their body measurements (hip height, hip width, head length, croup height). This result can be used as basic data for the development of horse type determination (racing, riding, Riding for the Disabled, Working, or fattening) through the analysis of body composition, and be utilized as the basic data for the producing and breeding new domestic riding horses through the 3D Stereosocpic image system analyze.

• Tuberculosis and Respiratory Diseases
• /
• v.38 no.3
• /
• pp.270-279
• /
• 1991

It has been well known that the integrity of airway epithelium is important in developing of bronchial hyperreactivity or bronchial asthma. But the mechanisms underlying this nonspecific airway hyperresponsiveness are not yet determined. To evaluate the ability of guinea pig trachea to release an epithelium derived relaxing factor (EpDRF) which relax rat vascular smooth muscle, we performed the coaxial bioassay using guinea pig trachea and rat aorta. And to evaluate the nature of EpDRF we investigate the influence of methylene blue and indomethacin on the coaxial bioassay. Results were as follows. 1) Vascular smooth muscle mounted into the epithelium intact trachea which was precontracted with phenylephrine was relaxed by addition of histamine or acetylcholine. But vascular smooth muscle mounted into epithelium denuded trachea failed to be relaxed. 2) Epithelium dependent relaxation of vascular smooth muscle was not affected by pretreatment of methylene blue or indomethacin. These results strongly suggests that guinea pig tracheal epithelium releases EpDRF which is able to relax rat vascular smooth muscle. And EpDRF released by airway epithelium is not related to endothelium derived relaxing factor (EDRF) or cyclooxygenase products.

• Bulletin of the Society of Naval Architects of Korea
• /
• v.54 no.4
• /
• pp.39-46
• /
• 2017

Jack-up drilling rigs are widely used in offshore oil and gas exploration industry. It is originally designed for use in the shallow waters less than 60m of water depth; there is growing demand for their use in deeper water depth over 150m and harsher environmental conditions. In this study, global in-place analysis of jack-up rig leg for North-sea oil well is performed through numerical analysis. Firstly, environmental conditions and seabed characteristics at the North-sea are collected and investigated measurements from survey report. Based on these data, design specifications are established and the overall basic design is performed. Dynamic characteristics of the jack-up rig for North-sea are considered in the global in-place analysis both leg and hull and the basic stability against overturning moment is also analyzed. The structural integrity of the jack-up rig leg/hull is verified through the code checks and the adequate safety margin is observed. The uncertainty in jack-up behaviour is greatly influenced by the uncertainties in the soil characteristics that determine the resistance of the foundation to the forces imposed by the jack-up structure. Among the risks above mentioned, the punch-through during pre-loading is the most frequently encountered foundation problem for jack-up rigs. The objective of this paper is to clarify the detailed structure and installation engineering matters for prove the structural safety of jack-up rigs during operation. With this intention the following items are addressed; - Characteristics of structural behavior considering soil effect against environmental loads - Modes of failure and related pre-loading procedure and parameters - Typical results of structural engineering and verification by actual measurement.

• Korean Journal of Plant Resources
• /
• v.27 no.5
• /
• pp.401-414
• /
• 2014

In an attempt to evaluate licorice quality based on its biological activity, we grafted an on-line high-performance liquid chromatography (HPLC) bioassay method into the previously established HPLC analysis method. The common antioxidant peaks in licorices of various origin were observed through an on-line HPLC/DPPH system leading to a decrease in absorbance at 517 nm for 2,2-diphenyl-1-picrylhydrazyl (DPPH). Among them, the licorice from Youngju possessed the highest activity. Therefore, three active standard compounds from the dehydroglyasperin C, dehydroglyasperin D, and isoangustone A, were isolated and elucidated by medium pressure liquid chromatography (MPLC) and instrumental analysis such as nuclear magnetic resonance (NMR), respectively. On-line HPLC/ABTS analysis method with the simultaneous determination of three standard compounds and their radical scavenging activity was established for the quality evaluation of licorices. 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radicals (ABTS) which is stable and effective was used in replace of DPPH. The radical scavenging activity of three standards is compared with that of Trolox, known as antioxidant, showing a negative peak with a decrease in absorbance at 734 nm for ABTS. This on-line HPLC/ABTS analysis method was validated for specificity, linearity, precision and accuracy in compliance with international conference on harmonization (ICH) guideline.

• Korean Journal of Head & Neck Oncology
• /
• v.18 no.1
• /
• pp.11-17
• /
• 2002

Objectives: Expression of HMGI(Y), a nucleoprotein that binds to A/T rich sequences in the minor groove of the DNA helix, is observed in neoplastically transformed cells but not in normal cells. We have analyzed HMGI(Y), p53 expression and Ki-67 labelling index in squamous cell carcinomas of the head and neck, and evaluated its clinicopathologic significance. Materials and Methods: 40 cases of squamous cell carcinoma of the head and neck were entered on the study of immunohistochemical stains for HMGI(Y), p53 and Ki-67. We analyzed the relationship between HMGI(Y), p53, Ki-67 expression and age, sex, primary tumor site, stage, survival rate, recurrence. Results: HMGI(Y) expression evidenced by immunohistochemical staining was observed in 35 of 40 (87.5%) squamous cell carcinoma of the head and neck. But no significant correlation was observed between HMGI(Y) expression and other clinical factors such as primary site, tumor stage, differenciation, cervical lymph node, metastasis, recurrence and immunohistochemical status of p53. The Ki-67 labelling index was significantly correlated with recurrence and HMGI(Y) expression (p<0.05). Conclusion: This results suggest the Ki-67 is a good prognostic factor and the HMGI(Y) expression plays some roles in carcinogenesis and cellular proliferation of squamous cell carcinoma of the head and neck. HMGI(Y) gene can be used as a cancer marker, the correlation between the gene expression and the prognosis of the cancer patient should be proved in the future studies.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.32 no.6
• /
• pp.798-802
• /
• 1999

This study was performed to compare the effect of enrichement of rotifer and Artemia fed marine bacteria (Erythrobacter sp. S$\pi$-1) with those fed the different diets-(Super Selco, $\omega$-yeast and marine Chlorella) on the slime flounder larvae, Microstomus achne. Because the ratio of docosahexaenoic acid (DHA) to fatty acids in rotifer (dry weight $\%$) fed on Super Selco and ESP-SR including Erythrobacter sp. S$\pi$-1 was higher than that in rotifer fed marine Chlorella, the growth of the slime larvae fed the former showed better than the latter. And the ratio of DHA and highly unsaturated fatty acid (n-3 HUFA) to fatty acids in Artemia (dry weight $\%$) enriched by ESP-A including Erythrobacter sp. S$\pi$-1 were also higher than those in Artemia enriched by others or Artemia without enrichment. Larvae fed on Artemia enriched by ESP-A showed better growth and survival rate than those fed on Artemia enriched by others or Artemia without enrichment. With regard to dietary value of Erythrobacter sp. S$\pi$-1, its use could improve the quality of the live foods for the slime flounder larvae.