• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.4
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• pp.317-323
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• 2009

Chronic exposure of solar ultraviolet (UV) light to human skin results in photoaging with wrinkle formation. This study was performed to investigate anti-wrinkle effects of Lindera obtusiloba extract (LO) on UVB-induced wrinkle formation. We first measured cell proliferation and type I pN collagen synthesis activities in normal human dermal fibroblasts. Cell proliferation and type I pN collagen synthesis were increased by 33.8 % and 91.8 %, respectively, compared with no treatment control. SKH-1 hairless mice were topically applied 5 % LO solution for 10 weeks with UVB irradiation three times a week. After 10 weeks, a visual assessment and replica assay were performed on each mouse. According to visual assessment of close-up photos and skin replica, application of 5 % LO solution inhibited UV-induced wrinkle formation in mouse skin as compared to the vehicle-applied control mice. These results indicated that LO could protect skin wrinkle formation caused by chronic photo-irradiation in hairless mice.

• Korean Journal of Pharmacognosy
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• v.47 no.1
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• pp.49-54
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• 2016

In our search for natural products affecting blood circulation, the n-butanol fraction from whole plant of Glehnia littoralis Fr. Schm. (GLB) improved blood lipid parameters, and ameliorated obesity in high-fat-diet (HFD)-fed C57BL/6 mouse model. Hyperlipidemia was induced by high-fat-diet for 4 weeks, and then GLB was orally administrated with 400 mg/kg/day for 4 weeks. GLB-treated group showed that the gain in body weight was significantly attenuated, the levels of total cholesterol and triglyceride significantly lowered on blood chemical analysis, and significantly prolonged the mice bleeding time when compared with those of HFD control group. Concomitantly, phytochemical composition of GLB was investigated by HPLC-hyphenated spectroscopy, and two major phenolic compounds, rutin and chlorogenic acid were identified in the GLB. Taken together, these results indicate that GLB has cardiovascular protective effects and could be a natural medicine candidate for the prevention of cardiovascular disease.

• The Korean Journal of Zoology
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• v.32 no.4
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• pp.305-313
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• 1989

The present investigation aims at inquiring into a possible target for the heat inactivation of SCK tumor cells by comparing the kinetics of cell survival, rate of protein synthesis, and DNA polymerase activity in the presence of heat protector or heat sensitirer. A possible conclusion to be drawn from the present experiment is that there is no direct correlation between cell death and decrease in the rate of protein synthesis, but that the loss of DNA polvmerase $\beta$ activity correlates quite well with cell inactivation. Thus, protein degrada-tion and/or abnormal protein synthesis causes cell inactivation innireuv, possibly by altering the cellular environment which in turn affects the DNA polymerase $\beta$ activity. Accordingly, further studies, dealing with the correlation between changes in the cellular environment and DNA polymerase $\beta$ activity, are needed to set insight into a possible target for the heat inactivation of cells. 본 연구는 열보호제 또는 열증감제의 존재하에서 세포 생존곡선, 단백질 합성률, DNA 중합효소 $\beta$의 활성변화를 비교 검토함으로써 SCK 종양세포가 열에 의해서 불활성화될 때의 표적이 무엇인지를 밝혀보기 위해서 수행되었다. 본 실험의 결과로 추정하건대 열에 의한 세포치사는 단백질 합성률의 변화와는 직접적인 연관성이 없으나, DNA 중합효소 $\beta$의 활성도와는 밀접한 연관성이 있음을 알 수 있다. 즉, 단백질의 분해 또는 비정상적인 단백질의 합성이 세포의 환경을 변화시키고 이것이 DNA 중합효소 $\beta$의 활성에 영향을 미침으로써 간접적으로 세포의 치사를 초래할 것으로 짐작할 수 있다. 따라서, 세포의 열불화성화의 표적을 좀더 분명히 밝히기 위해서는 세포의 환경변화와 DNA 중합효소 $\beta$의 활성과의 관계를 추구하는 연구가 수행되어야 할 것으로 사료된다.

• Korean Journal of Acupuncture
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• v.30 no.1
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• pp.73-80
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• 2013

Objectives : LI11 has been known to suppress epileptic seizure. Using an mouse epilepsy model, we investigated whether acupuncture stimulation at LI11 can suppress kainic acid(KA)-induced epileptic seizure and apoptosis in the mouse hippocampus. Methods : Eight-week-old male C57/BL6 mice(20~25 g) were given acupuncture at LI11 or ST36 once a day for 3 days. After the last acupuncture stimulations, KA(30 mg/kg) was injected intraperitoneally and the degree of seizure was observed for 90 minutes. Twenty-four hours after KA administration, mice were sacrificed and the neural cell death, astrocyte activation and caspase-3 expression in their hippocampi were investigated. Results : Acupuncture stimulation at LI11 suppressed KA-induced epileptic seizure, neuronal cell death, astrocyte activation and caspase-3 expression. Conclusions : Acupuncture stimulation at LI11 decreases the KA-induced epileptic seizure and protects hippocampal cell death via regulating astrocyte activation and caspase-3 expression.

• Korean Journal of Environment and Ecology
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• v.7 no.2
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• pp.207-212
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• 1994

The census of Amphibia fauna in Tokyusan National Park was carried out during April 10, 1993 and August 8 - August 9, 1993 at the areas of construction field of golf course in Muju Resort and the Chibong. There were 6 species of 5 genera on the 4 families, 2 orders of Amphibia in Tokyusan National Park including Hynobius leechii. Onychodactylus fischeri, and Bufo bufo gargarizans which were announced the specific wild animals with the object of conserving by The Ministry of Environment. At the area of construction field of golf course in Muju Resort, there were 5 species of Amphibia observed as Hynobius leechii, Bombina orientalis, Bufo bufo gargarizans, Rana nigromaculata, and Rana dybowskii. Onychodactylus fischeri and Rana dyhowskii were observed at the Chibong area. The Chibong inwhich was observed 9 relatively large number of individuals of Onychodactylus fischeri was revealed as an environmentally sound habitat to Onychodactylus fischeri. Whereas in the construction field of golf course, there were intended to diminishing species and population size rapidly by progress with construction works. Therefore the large construction works such as golf couse and ski slopes which were concerning to deteriorate the natural environment should be restrain in National Park for animal diversity conservative.

• Korean Journal of Environment and Ecology
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• v.11 no.4
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• pp.535-557
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• 1998

설악산국립공원은 1965년에 천연보호구역, 1970년에 국립공원 제 5호, 1982년 유네스코에 의해 생물권보존지역으로 지정된 지역이다. 우리 나라 국립공원에서는 물론 다른 자연보존지역에서도 찾아볼 수 없는 중요성이 강조된 지역이나 연간 400만명의 이용객이 집중되기도 하는 지역이다. 우리 나라 국립공원은 본래 관광개발을 위해 지정된 동기를 갖고 있어 자연생태계 및 자연경관도 중요하지만 현실적으로는 합리적인 이용도 중요하다. 그러나 지금까지 국립공원은 자연보존에 대한 관리는 도외시 한 채 이용객 집중에 의해 발생되는 도로, 시설물, 건축물, 각종 오염에 대한 대책마련도 제대로 하지 못하여 국립공원하면 국민은 '등산이나 하고 값싼 음식점과 여인숙 수준의 숙박시설이 있는 관광지'정도로 인식하고 있다. 지난 30년간 압축고도성장을 해 온 우리 나라는 국토의 자연환경이 수없이 훼손되고 또한 옥외 레크레이션활동의 증가로 환경오염이 계속 증가되었다. 설악산국립공원도 예외는 아니어서 저앗ㅇ부와 등산로 주변생태계, 계곡생태계 등이 오염되어 식물, 포유동물, 물고기, 곤충 등에 영향이 나타나고 있지만 대피소, 휴게소, 숙박시설을 중심으로 한 오염행위는 계속 늘어만 가고 있다. 이제 설악산국립공원은 거의 위기상황에 도달된 느낌이다. 이런 시기에 환경생태학회 국립공원분과위원회 회원들은 `96년 외설악 지역, `97년 내설악지역을 2년 동안 각 분야에 걸쳐 조사를 수행하였다. 지난 12년간 외부의 지원도 없이 연구비를 자체 조달하여 힘들게 국립공원 연구를 진행하여 온 일환으로 설악산국립공원 조사를 2년 동안 수행한 것이다. 이번 조사는 `96년 가을 북한공비침투로 많은 지역에 대한 출입이 통제되어 외설악연구의 일부가 누락되었음을 첨언한다. 본 글에서는 2년 동안 본 회원들이 조사한 내용에 의해 설악산 국립공원의 현황을 분석하고 관리개선 방향을 제시하였다.

• The Korean Journal of Zoology
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• v.32 no.2
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• pp.134-141
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• 1989

The present investigation aims at elucidating a possible mechanism of heat inactivation of SCK ceils by comparing the kinetics of cell lethality and protein degradation in the presence of heat protector or heat sensitizer. The effect of heat sensitizer and protector was exhibited in both cell survival and protein degradation kinetics, the magnitude of the effect being much profound for the protector compared to the sensitizer. A conclusion to he drawn from the present experiment is that there is no direct correlation between cell lethality and protein degradation. Rather, protein degradation, which might occur in the membrane, causes cell inactivation indirectly, possibly by altering the cellular environment. Accordingly, further studies are needed to get insight into the mechanism of cell inactivation by hyperthermia.

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.329-335
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• 2008

This study was intended to research into the possibility of corilagin (CRN) isolated from Euphorbia helioscopia as rheumatoid arthritis drug. For the study, CRN was medicated to the abdominal cavity of collagen induced arthritis (CIA) mice that was a animal model for rheumatoid arthritis and its effects on incidence and arthritis index (AI) were studied. The results were as folllows; Medicating corilagin that were isolated from Euphorbia heliscopia to CIA mice resulted in inhibiting an incidence of arthritis and reducing in arthritis index. It was found that inflammatory cells were remarkably decreased and joint cavity was well secured. Also it was displayed that the formation of pannus was not observed and a cartilage was preserved well by cell histological research. Performing fluorescence flow cytometry of CIA mice resulted in reducing in inflammatory cells infiltrated each tissue.

• Korean Journal of Animal Reproduction
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• v.20 no.3
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• pp.233-238
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• 1996

This study was conducted to investigate the effects of cryoprotective agents and thawing temperature on the survival rate of the bisected embryos of mouse. The results of this study were summairzed as follows: 1. In in vitro culture after frozen with DMSO, glycerol, ethylene glycol or propanediol and thawed, the rates of normally developed bisected morula which was denuded were 31.7, 39.1, 28.0 and 23.1%, respectively. 2. The survival rates of bisected morula encased into the zona pellucida in in vitro culture after frozen with DMSO, glycerol, ethylene glycol or propanediol and thawed were 72.4, 68.7, 64.0 and 59.5%, respectively. 3. The survival rates of bisected denuded blastura in in vitro culture after frozen with DMSO, glycerol, ethylene glycol or propanediol and thawed were 48.3, 44.8, 32.1 and 28.6%, respectively. 4. The survival rates of bisected blasturae encased into the zona pellucida in in vitro culture after frozen with DMSO, glycerol, ethylene glycol or propanediol and thawed were 73.6, 67.4, 53.0 and 49.1%, respectively. 5. In in vitro culture after frozen with DMSO, glycerol, ethylene glycol or propanediol and thawed at room temperature, the rates of the normally developed bisected morula which was encased into the zona pellucida were 67.1, 62.3, 57.7 and 53.0%, respectively, and those of the bisected blasturae encased into the zona pellucida were 70.8, 65.4, 56.6 and 52.1%, respectively. 6. The survival rates of bisected morula which was encased into the zona pellucida in in vitro culture after forzen with DMSO, glycerol, ethylene glycerol or propanediol and thawed at 37$^{\circ}C$ were 74.3, 71.3, 63.9 and 57.4%, respectively, and those of bisected blastura encased into zona pellucida were 76.0, 69.1, 61.1 and 56.1%, respectively.

• Journal of Sasang Constitutional Medicine
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• v.26 no.2
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• pp.165-179
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• 2014

Objectives To evaluate the neuroprotective effects of Hyangsayangwi-tang (HY), a Korean traditional medicinal prescription in a Parkinson's disease mouse model. Methods Four groups(each of 10 mouse per group) were used in this study. The neuroprotective effect of HY was examined in a Parkinson's disease mouse model. C57BL/6 mouse treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30mg/kg/day), intraperitoneal (i.p.) for 5 days. Slow behavioral responses and memory disorder is the major clinical symptoms of PD. In order to investigate the effect of HY on recovery of behavioral deficits and memory, we examined the motor function and memory by using Morris water maze and Forced swimming test. Ischemic mouse brain stained with TTC(2,3,5 triphenyl tetrazolium chloride) in the MPTP-induced Parkinson's disease to find out ischemia and tissue damage in mouse. The convenient, simple, and accurate high-performance liquid chromatography (HPLC) method was established for simultaneous determination of neurotransmitters in MPTP-HY group. To measure the amount of dopamine in mice brain, striatum-substantia nigra, was examined by Bradford assay. Immunohistochemistry was examined in the MPTP-induced Parkinson's disease (PD) mouse to evaluate the neuroprotective effects of Hyangsayangwi-tang on hippocampal lesion, ST and SNpc. Results and Conclusions Hyangsayangwi-tang (HY) prevents MPTP-induced loss of serotonin, hippocampus and TH-ir cell.