Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Acupuncture Stimulation at LI11 Suppresses Seizure and Apoptosis in Hippocampi on an Epilepsy Mouse Model

간질 동물 모델을 이용한 곡지(曲池) 및 족삼리(足三里)의 간질발작 및 해마 신경세포 보호 효과 비교 연구

  • Lee, Jong Boon (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Hwang, Kyoung Min (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Yoo, Tae-Won (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Bae, Chang-Hwan (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Kwon, Sunoh (Acupuncture & Meridian Science Research Center, Kyung Hee University) ;
  • Kim, Seung-Tae (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University)
  • 이종분 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 황경민 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 유태원 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 배창환 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 권선오 (경희대학교 침구경락과학연구센터) ;
  • 김승태 (부산대학교 한의학전문대학원 경락구조의학부)
  • Received : 2013.03.11
  • Accepted : 2013.03.20
  • Published : 2013.03.27
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Abstract

Objectives : LI11 has been known to suppress epileptic seizure. Using an mouse epilepsy model, we investigated whether acupuncture stimulation at LI11 can suppress kainic acid(KA)-induced epileptic seizure and apoptosis in the mouse hippocampus. Methods : Eight-week-old male C57/BL6 mice(20~25 g) were given acupuncture at LI11 or ST36 once a day for 3 days. After the last acupuncture stimulations, KA(30 mg/kg) was injected intraperitoneally and the degree of seizure was observed for 90 minutes. Twenty-four hours after KA administration, mice were sacrificed and the neural cell death, astrocyte activation and caspase-3 expression in their hippocampi were investigated. Results : Acupuncture stimulation at LI11 suppressed KA-induced epileptic seizure, neuronal cell death, astrocyte activation and caspase-3 expression. Conclusions : Acupuncture stimulation at LI11 decreases the KA-induced epileptic seizure and protects hippocampal cell death via regulating astrocyte activation and caspase-3 expression.

Keywords

References

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