• Journal of Bio-Environment Control
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• v.18 no.3
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• pp.208-214
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• 2009

In this study, we investigated the effects of the number of air exchanges (NAE) on shape fruit body in two oyster mushrooms, Chunchu 2# and Suhan 2#. The suitable NAEs of Chunchu 2# were $1/10h^{-1}$ at primordial induction, $1/6h^{-1}$ at early stage of growth, $1/4h^{-1}$ at middle stage of growth, $1/4h^{-1}$ at late stage of growth and those of Suhan 2# were $1/6h^{-1}$ at primordial induction, $1/6h^{-1}$ at early stage of growth, $1/4h^{-1}$ at middle stage of growth, and $1/2h^{-1}$ at late stage of growth. In those conditions, the fruit bodies grew well. $CO_2$ concentration hardly affected the primordial formation of both mushrooms. However there were ventilation disturbances over 1500ppm. For instance, the end of pileus rolled up etc. As a result, Suhan 2# required higher ventilation compared with Chunchu 2# and the lower NAE was favorable for growth.

• The Korean Journal of Mycology
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• v.24 no.4 s.79
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• pp.322-328
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• 1996

It is possible to cultivate Pleurotus ostreatus on the crush medium of Reynoutria sachalinensis. The crush of leaves and stems of Reynoutria sachalinensis with water (1:8 W/V) enhanced mycelial growth of P. ostreatus. That mycelial growth of P. ostreatus on the crush medium was accelerated three times as fast as that on malt extract agar (MEA), and mycelial compactness was denser than that of on MEA. The same result was obtained on mixture of saw dust and the crush of leaves and stems in test tube and bottle. The addition of rice bran and the crush to saw dust was best for mycelial growth. Regardless of pH (4.5, 6.5 and 8.5), P. ostreatus could suppress the growth.

• Journal of Mushroom
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• v.21 no.4
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• pp.222-227
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• 2023

Mushrooms have a unique taste and aroma, so in the processing of mushroom products with other ingredients, a separate pre-processing step is often taken to eliminate the mushroom aroma. In this study, we analyzed the changes in the concentration of volatile compounds according to drying conditions to promote the activation of processing using the fruiting bodies of yellow oyster mushrooms(Pleurotus citrinopileatus) and pink oyster mushrooms(P. djamor). The caps and stipes of yellow oyster and pink oyster mushrooms were separated and freeze-dried at -70℃ for 120 hours. Subsequently, they were hot air-dried at temperatures of 40, 50, 60, and 70℃ for 24, 24, 16, and 12 hours, respectively. The dried samples were pulverized and quantitatively analyzed by SPME-GC-MS. In the case of yellow oyster mushrooms, the concentration of t-2-nonenal in caps and stipes during freeze-drying was 164.43 ㎍/g d.w. and 174.80 ㎍/g d.w., respectively, whereas during hot air-drying, it significantly decreased to 0.35~3.41 ㎍/g d.w. and 0.98~59.88 ㎍/g d.w. In a similar manner, for pink oyster mushrooms, the concentration of 1-octen-3-ol during freeze-drying in caps and stipes was 31.05 ㎍/g d.w. and 176.17 ㎍/g d.w., respectively, whereas during hot air-drying, it significantly decreased to 1.59~9.66 ㎍/g d.w. and 1.96~15.77 ㎍/g d.w. Furthermore, most volatile compounds showed a tendency to decrease in concentration as the temperature during hot air-drying increased.

• Journal of Mushroom
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• v.2 no.4
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• pp.222-224
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• 2004

Sodam oyster mushroom(Pleurotus ostreatus were bred in Mushroom Research Institute, Gyonggi Province A.R.E.S in 2002. Sodam oyster mushroom were bred and cultivated one after mating single spores collected from Aenutari 1-ho and Suhan 1-ho. The major characteristics of the mushroom are showing a lot of pinheadings, the gray-colored and shallow cornhead-shaped pileus. Around 20 days at $20^{\circ}C$ were required for incubation of Sodam and the yield was shown high by 148.2g/bottle. As the activity of hypha is so strong, the proper time of pinheading is demanded.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.338-342
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• 1994

Amino acids of Pleurotus ostreatus in various cultural media(rice straw, sawdust and cotton waste) were analyzed to recognize the compositional differences depending on pileus size and portions(pileus and stipe) Total amino acids of rice straw were a little less than that of sawdust and cotton waste. Seventeen amino acids were identified and quantified. Among the amino acids glutamic acid was higest and cystine was lowest. The amino acid contents depending on the pileus size was higest in $3{\sim}7\;cm$ of rice straw, under 3 cm of sawdust, over 7 cm of cotton waste, respectively. The content of them was found to be higher in pileus than stipe.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2003.04a
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• pp.152-156
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• 2003

우리나라 시설원예 면적은 최근 정체되는 기미를 보이긴 하지만, 아직도 시설원예 면적이 50,000ha 이상에 달하고 난방면적도 점점 증가되어 전체 면적 중에 난방면적이 차지하는 비율은 현재 12,300ha 정도인 24%이다. 난방기의 보급기의 보급도 매년 증가하여 '00년말 현재 127,560대 정도이다. 최근 국내 버섯 재배면적의 42% 차지하는 느타리(큰느타리 포함)버섯 재배사도 연중재배를 위하여 난방을 실시하는 농가가 점점 늘고 있고, 특히 새송이(큰느타리) 버섯 재배사의 대부분은 간이형 온실에서 냉난방 설비를 갖추어 연중재배를 하고 있다. (중략)

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.02a
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• pp.311-316
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• 2002

느타리버섯의 포장상자를 예냉·저온유통에 적합한 구조로 개선하기 위하여 4kg용 골판지상자를 T11형 표준 팔레트에 적재율이 96%이상 되게 제작하여 고습조건에서도 안전하게 느타리버섯을 보호할 수 있는 상자구조를 찾기 위한 수직압축강도시험과 냉각성능 및 예냉후 저온저장 중 품질변화에 대한 시험을 실시하였다. 시험결과 4kg 접음식 상자에서 내 포장상자를 사용할 경우 고습조건에서 안전압축강도를 유지할 수 일었으며, 브릿스형 상자에 비하여 경제적으로 제작할 수 있고, 또한 홈판형 상자에 비하여 냉각속도를 단축하고 균일한 냉각을 할 수 있어 느타리 버섯의 품질유지에 유리한 것으로 나타났고, 기존 유통되는 상자을 사용할 때 보다 유통기간을 4∼5일정도 연장시킬 수 있는 것으로 나타났다.

• Journal of Mushroom
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• v.2 no.4
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• pp.225-227
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• 2004

Hukbaek oyster mushroom were bred and cultivated one after mating spores from Aenutari 1-ho irradiated to X-ray and Byungnutari 1-ho. The major charateristics of the mushroom are showing that pileus is black-brown and stems are pure-white and straight. The optimum temperature for the mycelial growth was around $25{\sim}30^{\circ}C$ and that for the pinheading and growth of fruitbody was around $14{\sim}16^{\circ}C$. Around 20 days at $20^{\circ}C$ were required for incubation time and the yield was shown high by 142.8g/bottle. When the cultivating temperature is over $17^{\circ}C$ the color of pileus becomes gray-brown.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.05a
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• pp.52-56
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• 2000

느타리 버섯의 다양한 품종과 재배형태가 급변히 요구되는 시기에 충남농업기술원 버섯연구팀의 5년 (1996～2,000)에 걸쳐 국,내외 수집 20 균주를 공시하여 개발 육성한 청풍과 명월 신품종 느타리버섯을 현재 재배되고 있는 기존 느타리품종과 동일여부를 위해 PCR-RAPD분석과 배양적, 재배적 특성을 인공재배 시험한 결과를 요약하면 다음과 같다. 1. 청풍, 명월느타리버섯 균사배 양 온도는 청 풍은 20～$25^{\circ}C$,명 월은 25～3$0^{\circ}C$, 이고 배지는 PDA배지가 양호하고 균사생육은 청풍, 명월 모두 비슷하였으며 배지 pH는 6.5～7.5 였다. 2. 버섯재배가능은 청풍은, 5～26$^{\circ}C$ 명 월은, 7～28$^{\circ}C$로 온도범위가 넓어 재배가 용이하나 적정 생육온도는 청풍, 명월 공히 15～19$^{\circ}C$가 양호 하였다 3. 청풍과 명월의 배양온도 23$^{\circ}C$에서 균사배양기간 23～24일 (850cc/pp),폐면 균상 재배에서 균배양 기간은 청풍, 명월 공히 11～12일, 초발이 소요일수는 2～3일 소요 되었다. 4.초발이시 청풍과 명월 갓 색택은 진청색이고, 생육후기 청풍은 회갈색, 명월은 진회색으로 조직이 치밀하고 저장성이 양호하여 경제성이 기대된다. 5. 다발수는 춘추2호 (13개/다발) 대 비 청 풍11개, 명월12개로 약간 적었으나 개체중은 춘추2호(14g/개) 대비 청풍 16g/개, 명월 14g/개로 비슷한 경향 이었다. 6. 수량성은 균상 폐면재배시 춘추2호 (40kg/3.3$m^2$)대비 청풍 느타리버섯은 (46kg/3.3$m^2$) 15%증수되고, 명월느타리버섯은(41kg/3.3$m^2$)로 3% 증수되었으며 병재배 겸용도 가능하였다. 7. PDA대치배양과 PCR-RAPD시험에서 청풍, 명월 신품종느타리 버섯은 기존 재배 되고 있는 느타리품종과 다른 품종으로 확인 되었다.

• The Korean Journal of Mycology
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• v.24 no.2 s.77
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• pp.155-165
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• 1996

This study was carried out to identify the phylogenetic relationship among several isolates of Pleurotus species by comparing ITS II region of ribosomal DNA(rDNA) repeat unit. Two primers from ribosomal DNA sequences were chosen to amplify the specific internal transcribed spacer (ITS) II region of Pleurotus spp. The exact ITS II region with an unique band from six species of Pleurotus genus could be amplified using the two primers taken from at the 3'-end of 5.8S rDNA and 5'-end of 28S rDNA. Six representative species of the Pleurotus genus were easily characterized according to the length differences of ITS II region. Furthermore, within P. ostreatus species, different sizes of ITS II region could be observed in the isolates of ASI 2025 and ASI 2095 although they were classified as P. ostreatus by the conventional observation. The nucleotide sequence analyses of PCR-amplified ITS II region indicated that the isolates ASI 2025 and ASI 2095 were different from other Pleurotus spp. When the nucleotide sequences of six Pleurotus species were compared, three typical ITS II regions were highly variable especially at both ends of this region. The phylogenetic tree obtained by the Neighbor program of Felsenstein PHYLIP package with all the nucleotide sequence of Pleurotus spp. indicated that P. ostreatus, P. florida, P. sajor-caju and P. eryngii were closely related to one phylogenetic branch and P. cystidious was related to other branch with P. cornucopiae. The isolates ASI 2025 and 2038, however, were not closely related to any other Pleurotus spp. and formed their own individual branches.