• Journal of Life Science
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• v.25 no.9
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• pp.1007-1013
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• 2015

A COG (Cluster of Orthologous Groups of proteins) algorithm was applied to detect conserved genes in 711 prokaryotes. Only COG0080 (ribosomal protein L11) was common among all the 711 prokaryotes analyzed and 58 COGs were common in more than 700 prokaryotes. Nine COGs among 58, including COG0197 (endonuclease III) and COG0088 (ribosomal protein L4), were conserved in a form of one gene per one organism. COG0008 represented 1356 genes in 709 of the prokaryotes and this was the highest number of genes among 58 COGs. Twenty-two COGs were conserved in more than 708 prokaryotes. Of these, two were transcription related, four were tRNA synthetases, eight were large ribosomal subunits, seven were small ribosomal subunits, and one was translation elongation factor. Among 58 conserved COGs in more than 700 prokaryotes, 50 (86.2%) were translation related, and four (6.9%) were transcription related, pointing to the importance of protein-synthesis in prokaryotes. Among these 58 COGs, the most conserved COG was COG0060 (isoleucyl tRNA synthetase), and the least conserved was COG0143 (methionyl tRNA synthetase). Archaea and eubacteria were discriminated in the genomic analysis by the average distance and variation in distance of common COGs. The identification of these conserved genes could be useful in basic and applied research, such as antibiotic development and cancer therapeutics.

• Journal of Animal Science and Technology
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• v.46 no.1
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• pp.91-96
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• 2004

It is generally believed that amino acids occurring naturally in mammals are of the L-configuration. D-amino acid(DM) are common in nature as constituents of bacterial cell walls and several antibiotics. Recent reports have demonstrated the presence of small amounts of free DM in milk. The presence of free DM may affect the food quality by decreasing the nutritional value. Our objective was to examine whether the free DM carne from psychrotrophic bacteria. Free DM was produced by treating raw milk with Pseudomonas spp. The samples were extracted with sulphosalicylic acid and derivatized with AccQ-$Tag^{TM}$ reagent when the analysis was carried out by reverse-phase HPLC. We tested correlations of the content of free DM with bacterial growth. Significant amounts of free D-a1anine and D-proline have been found in the raw milk inoculated with Pseudomonas spp. The increase of D-alanine and D-proline appeared to be mainly related to the presence of Pseudomonas fluorescens. These results suggest that free DM may be considered as an indicator of psychrotrophic bacterial milk contamination.

• Journal of Educational Research in Mathematics
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• v.23 no.2
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• pp.253-267
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• 2013

The newly revised mathematics curriculum of 2007 speaks of ultimate goal to develop ability to think and communicate mathematically, in order to develop ability to rationally deal with problems arising from the life around, which puts emphasize on mathematical communication. In this study, analysis on mathematical communication and analogical thinking process of group of students with similar level of academic achievement and that with different level, and thus analyzed if such communication has affected analogical thinking process in any way. This study contains following subjects: 1. Forms of mathematical communication took placed at the two groups based on achievement level were analyzed. 2. Analogical thinking process was observed through trapezoid's area obtaining activity and analyzed if communication within groups has affected such process anyhow. A framework to analyze analogical thinking process was developed with reference of problem solving procedure based on analogy, suggested by Rattermann(1997). 15 from 24 students of year 5 form of N elementary school at Gunpo Uiwang, Syeonggi-do, were selected and 3 groups (group A, B and C) of students sharing the same achievement level and 2 groups (group D and E) of different level were made. The students were led to obtain areas of parallelogram and trapezoid for twice, and communication process and analogical thinking process was observed, recorded and analyzed. The results of this study are as follow: 1. The more significant mathematical communication was observed at groups sharing medium and low level of achievement than other groups. 2. Despite of individual and group differences, there is overall improvement in students' analogical thinking: activities of obtaining areas of parallelogram and trapezoid showed that discussion within subgroups could induce analogical thinking thus expand students' analogical thinking stage.

• Korean Journal of Clinical Laboratory Science
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• v.47 no.4
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• pp.318-323
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• 2015

The aim of this study was to assess the association of metabolic syndrome and Vitamin D in Korean adults. The study subjects were Korean menopausal woman (n=4,340) who participated in the Korea National Health and Nutrition Examination Survey 2010~2012. After adjusting for factors such as age, body mass index, total cholesterol, smoking, and regular exercise, the mean 25(OH)D levels ($M{\pm}SE$) decreased with increasing metabolic syndrome score (MSS) (MSS 0, $18.18{\pm}0.29ng/Ml$; MSS 1, $18.09{\pm}0.21ng/mL$; MSS 2, $18.07{\pm}0.19ng/mL$; MSS 3, $18.04{\pm}0.21ng/mL$; MSS ${\geq}4$, $17.27{\pm}0.23ng/mL$), and the mean 25(OH)D level ($M{\pm}SE$) for metabolic syndrome ($17.66{\pm}0.16ng/mL$) decreased in comparison to non-metabolic syndrome ($18.11{\pm}0.14ng/mL$). In conclusion, our results suggest that an increase in metabolic syndrome score or metabolic syndrome are inversely associated with the vitamin D levels.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.6
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• pp.685-691
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• 1996

This experiment was conducted to investigate the effect of some soil conditioners, such as polyvinylalcohol(PVA), zeolite and perlite, on the changes of soil physical properties and on tobacco growth in paddy-upland rotated field. Soil conditioners were treated at the rates of 120kg in PVA, 500kg in zeolite and perlite per l0a, respectively. Ratio of soil aggregates formed from the treated plots tended to. be higher than those from the control in the order of PVA > perlite > zeolite. The wet aggregate stability, mean weight diameter, moisture retention and air permeability from the treated plots tended to be higher than those from the control. Amounts of water-stable aggregates of PV A-treated soil increased with higher soil moisture showing a peak at 50% of moisture content. But with respect to particle of size aggregate formed for crop growth and workability in field, it was presumed that 40% of soil moisture content would be most desirable. Visual characters of soil surface throughout the experiment clearly showed that treated soils were maintaining better surface roughness and porosity than control, but difference in water stable aggregates among treated plots tended to be narrowed. The growths of tobacco, espacially its root zone were better in conditioner treated plots than in non-treated plot showing best in PVA-treated soil.

• Korean Journal of Environmental Agriculture
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• v.21 no.4
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• pp.274-278
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• 2002

Nitrate removal rate in three wetland cells was examined. The acreage of each cell was 150 $m^2$. They were a part of a stream water treatment demonstration system which was composed of two ponds and six wetland cells. Earth works far the pond-wetland system were finished from April 2000 to May 2000 and reeds were planted in the three cells in May 2001. Waters of Sinyang Stream flowing into Kohung Esturiane Lake located southern coastal area of Korean Peninsula were pumped into a primary pont Effluents from a secondary pond were funneled into the three cells. Volumes and water quality of inflow and outflow were analyzed from July 2001 through December 2001. Inflow and outflow averaged 20 $m^3/d$ and 19.3 $m^3/d$, respectively. Hydraulic retention time was 1.5 days. Average influent and effluent nitrate concentration was 2.30 mg/L, 1.75 mg/L, respectively. Nitrate removal rate in the three cells averaged 80.9 $mg/m^2/day$. Seasonal changes of nitrate retention rates were closely related to those of wetland temperatures. Full growth of reeds within a few years can develope litter-soil substrates beneficial to the denitrification of nitrate, which may lead to increases of the nitrate retention rates.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.1
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• pp.1-8
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• 2010

Few management studies have been conducted on late planting date for corn silage in paddy field as a barley-corn forage cropping system. Experiments were conducted during 2007 and 2008 at the Chungnam Agricultural Research & Extension Services. The objectives of this study were to determine relationships between planting date and com forage yield and to determine the best hybrid at the delayed planting after whole crop barley's harvest. The treatments consisted of 2 planting dates and 7 hybrids. Delayed planting considerately reduced stem diameter, individual ear size and weight. The ratio of dried leaf and culm in aerial plants was increased and that of ear was decreased. The coefficient of variation in fresh, dry matter (DM) and total digestible nutrients (TDN) yield was higher at delayed planting date on cropping system with whole crop barley than that of planting at optimum season. So, fresh weight was reduced by 91.8%, dry matter by 72.6%, grain yield by 51.0% and TDN yield by 68.1%. The appropriate hybrid for delayed planting after whole crop barley harvest in middle region was 'Gangdaok' as lower in the reduction of ear size and weight compared to that of planting at optimum season. Reduction in grain yield caused to decrease the DM and TDN yields. Therefore, to gain stable dry matter in silage corn by delayed planting date on cropping system with whole crop barley was necessary to select com hybrid on the minimum reduction in ear size and weight.

• Research in Plant Disease
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• v.17 no.3
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• pp.317-325
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• 2011

Bacterial pustule of soybean (Glycine max) caused by Xanthomonas axonopodis pv. glycines is one of the most prevalent bacterial diseases of soybean in Korea, where it causes considerable yield loss. This study was carried out to develop yield prediction model for bacterial pustule by analyzing correlation between the percentage of diseased leaf area and yield. The severe disease incidence of soybean bacterial pustule caused yield losses by 19.8% in 2006 and 16.8% in 2007, respectively. Severity of bacterial pustule greatly affected on 100 seed weight and yield, but did not on stem length, number of branches per plant, number of pods per plant, number of seeds per plant. On the other hand, correlation coefficients between diseased leaf area and yield were $-0.93^*$('06) and $-0.77^*$('07), respectively. The regression equation obtained by analyzing correlation between the percentage of diseased leaf area and yield loss in 2006 and in 2007 was y = -3.2914x + 348.19($R^2$ = 0.8603) and y = -2.9671x + 302.08($R^2$ = 0.9411), respectively. These results will be helpful in estimating losses on a field-scale and thereby predicting the production of soybean.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.342-349
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• 2015

Kiwifruit is a new fruit crop that was commercialized in the late 1970s. Recently, its cultivation and consumption have increased rapidly worldwide. Kiwifruit is a dioecious, deciduous, and climbing plant having fruit with hairs and various flesh colors and a variation in ploidy level; however, the industry consists of very simple cultivars or genotypes. The need for efficient cultivar improvement together with the evolutional and biological perspectives based on unique plant characteristics, have recently encouraged genome analysis and bioinformatics application. The draft genome sequence and chloroplast genome sequence of kiwifruit were released in 2013 and 2015, respectively; and gene annotation has been in progress. Recently, transcriptome analysis has shifted from previous ESTs analysis to the RNA-seq platform for intensive exploration of controlled genetic expression and gene discovery involved in fruit ascorbic acid biosynthesis, flesh coloration, maturation, and vine bacterial canker tolerance. For improving conventional breeding efficiency, molecular marker development and genetic linkage map construction have advanced from basic approaches using RFLP, RAPD, and AFLP to the development of NGS-based SSR and SNP markers linked to agronomically important traits and the construction of highly saturated linkage maps. However, genome and transcriptome studies have been limited in Korea. In the near future, kiwifruit genome and transcriptome studies are expected to translate to the practical application of molecular breeding.

• Journal of Korean Society of Steel Construction
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• v.26 no.6
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• pp.511-522
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• 2014

In this study, an innovative steel stair system is presented which enables rapid erection and high quality control in both residential and office building construction. This system features two lightweight steel stringers of box shape, bolted connections easy to absorb construction tolerance, and stair steps movable transversely (or sliding steps) such that the work space needed for concrete stairway wall could be easily provided. In this type of stairway system, other than providing robust connecting details, ensuring vibration performance is especially important since this system may be vibration-sensitive due to lightweight nature and/or probable low damping. To tackle these issues, a series of full-scale mock-up tests were conducted by using box-shape stringer members with or without concrete-fill. The connection system was shown to be sufficiently stiff and strong, or it remained elastic even under the 160% of service load level. Among the seven stringer alternatives, five exhibited satisfactory vibration performance according to the related North American and European acceptance criteria.