• Title/Summary/Keyword: 과학적 기술

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Light Quality and Photoperiod Affect Growth of Sowthistle (Ixeris dentata Nakai) in a Closed-type Plant Production System (밀폐형 식물생산시스템에서 광질과 광주기에 따른 씀바귀의 생육)

  • Kim, Hye Min;Kang, Jeong Hwa;Jeong, Byoung Ryong;Hwang, Seung Jae
    • Horticultural Science & Technology
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    • v.34 no.1
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    • pp.67-76
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    • 2016
  • This study was conducted to examine the optimal environmental condition for promoting the growth of sowthistle as affected by light quality and photoperiod in a closed-type plant production system. Seeds were sown in 240-cell plug trays and then germinated for 3 days at a 24-hour photoperiod in a closed-type plant production system with LED lights (R:B:W = 8:1:1). Seedlings were transplanted and grown under 3 types of LED (R:B:W = 8:1:1, R:W = 3:7, or R:B = 8:2) and 4 photoperiods (24/0, 16/8, 8/16, or 4/20 hours) with $230{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ light intensity at a density of $20cm{\times}20 cm$. The experimental design was a randomized complete block design. Plants were cultured for 40 days un der the condition of $21{\pm}2^{\circ}C$ and $70{\pm}10%$ relative humidity after transplanting. Plants were fed with a recycling nutrient solution (pH 7.0 and EC $2.0dS{\cdot}m^{-1}$) contained in a deep floating tank. Fresh weight and dry weight of shoot or root, leaf length, and leaf area were the greatest in the photoperiod of 24/0 (light/dark) with RW LED. The highest number of leaves occurred in the photoperiod of 16/8 (light/dark) with RB LED, while the incidence of tip burn was higher in the photoperiod of 24/0 (light/dark) compared to the other treatments. Chlorophyll value was the highest in the 16/8 (light/dark) photoperiod and there was no significant difference by light quality. Chlorophyll fluorescence was the lowest in the photoperiod of 24/0 (light/dark) compared with other treatments. Therefore, in terms of economic feasibility and productivity for Ixeris dentata Nakai cultivation in a closed-type plant production system, the results obtained suggest that plants grew the best when kept in a photoperiod of 16/8 (light/dark) and light quality of combined LED RW (3:7).

Enhanced Resistance to Sulfur Dioxide Gas in Transgenic Petunia by Stacking both SOD2 and NDPK2 Genes (SOD2와 NDPK2 유전자 집적에 의한 페튜니아의 아황산가스 저항성 증진)

  • Lee, Su Young;Cheon, Kyeong-Seong;Kim, So Young;Kwon, O Hyeon;Lee, Hye Jin;Kim, Won Hee;Yoo, Bong Sik
    • Horticultural Science & Technology
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    • v.34 no.1
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    • pp.154-162
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    • 2016
  • The aim of this study was to develop a transgenic petunia with enhanced resistance to sulfur dioxide ($SO_2$) gas by stacking two genes, SOD2 and NDPK2, which are both known to confer resistance to abiotic stresses. The first-generation hybrids ($TF_1$) were obtained through reciprocal crosses between an SOD2-transgenic line SOD2-2-1-1-35($T_4$)[S($T_4$)] and an NDPK2-transgenic line NDPK2-7-1($T_2$)[N7-1($T_2$)]. Approximately 32.1-73.0% of the first-generation hybrids ($TF_1$) carried both SOD2 and NDPK2 genes. These hybrids showed 2.6 and 5.1 times less damage than hybrids carrying only SOD2 or NDPK2 genes, respectively, when they were treated with $SO_2$ gas at 30 ppm. This confirmed that the heterozygous hybrids were more resistant to $SO_2$ than the hybrids carrying either one of the resistance genes. Second-generation hybrids ($TF_2$) were obtained by selfing the $TF_1$ individuals. We confirmed the expression of the stacked genes in the $TF_2$ hybrids by phenotypic observation of their response to $SO_2$ gas at 30 ppm as well as using RT-qPCR and photosynthetic efficiency.

Horticultural Activities Using Colorful Food for the Improvement of Emotional Intelligence and the Reduction of Unbalanced Vegetable Diet of Young Children (유아의 채소편식 감소와 정서지능 향상을 위한 컬러푸드 원예활동)

  • Son, Hyo-Jung;Song, Jong-Eun;Son, Ki-Cheol
    • Horticultural Science & Technology
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    • v.33 no.5
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    • pp.772-783
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    • 2015
  • This study focuses on horticultural activities using colorful food to reduce diets unbalanced in vegetables and to increase emotional intelligence. Horticultural activities using colorful food vegetables were based on 'Health in Daily Life' in the '7th Kindergarten Curriculum'; to improve the dietary habits of the young children, the program was also linked with a parents' education program. The research was conducted with a total of 70 children from classes for four-year-olds in three child-care centers located in Seoul. The horticultural activities based upon nutrition education included activities using colorful food vegetables and nutrition education. For the nutrition education group, only nutrition education was provided, while neither horticultural activities nor nutrition education were provided to the control group. The study was conducted from September to December 2011. A total of twelve sessions were conducted once a week for 60 minutes each. According to the result, after the horticultural activities with colorful food vegetable were conducted, both the nutrition education group and horticultural activity & nutrition education group showed improvements in 'Nutrition Knowledge' compared to the control group. Regarding 'Unbalanced Diet Behaviors', the horticultural activities & nutrition education group showed meaningful decreases compared to the control group. Moreover for preference of fruits and vegetables, the horticultural activities & nutrition education group revealed meaningful improvements. In conclusion, colorful food vegetable horticultural activity could be an effective approach to resolve the imbalance of health caused by unbalanced diets as children who participated in the colorful food vegetable horticultural activities continued to respond spontaneously to the colors of vegetables and fruits and showed joy and kept voluntarily eating them.

Change of asparagine content in soybean sprouts by variety, root growth, and cultivation period (콩나물의 품종, 뿌리발생 및 재배일수에 따른 asparagine 함량 변이)

  • Jeong, Yeon-Shin;Park, Hee Joon;Dhakal, Krishna Hari;Lee, Jeong-Dong;Lee, In-Jung;Hwang, Young-Hyun
    • Current Research on Agriculture and Life Sciences
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    • v.26
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    • pp.63-69
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    • 2008
  • The varietal difference, effect of root amounts and cultivation duration on the asparagine content of soybean sprouts, which is known to have the excellent detoxifying effect of ethanol, were investigated for developed varieties and indigenous sprout lines to establish cultivation methods of increasing the asparagine content and to develop soybean varieties having high asparagine content. Some of the research results obtained are summarized as follows; 1. Range and mean of asparagine content of 174 germplasm were 0.38~1.67% and 0.99%, respectively, on fresh weight basis. 2. Developed sprout-soybean varieties showed somewhat higher asparagine content of 1.29% than that of indigenous sprout lines of 0.96% on fresh weight basis. 3. No significant difference in asparagine content among the seed size groups was recognized though the highest asparagine content, 1.02% on fresh weight basis was observed in the seed size of 8.1~10.0g/100 seeds. 4. Among the seed coat color groups, soybean of brown seed coat color showed the highest asparagine content (1.15%) on fresh weight basis. No difference was observed among other groups of seed coat color. 5. Range of asparagine content of 174 varieties was 4.08~6.24% on dry weight basis. Soybean varieties that showed high asparagine content were Dawonkong, Sobacknamulkong, Sowonkong, and Somyungkong, with the contents of 6.24%, 6.21%, 5.95%, and 5.85%, respectively. 6. Amount of roots which have the highest asparagine content out of sprout parts was greatly increased up to 10 days of sprout cultivation. 7. Highly significant difference in asparagne content of sprout was recognized between those grown for five and ten days, with the asparagine content of 0.68% and 1.21%, respectively, on fresh weight basis. 8. Asparagine content of hypocotyle part was also highly proportional to days to cultivation; it increased from 2.91% at 2 days of cultivation to 15.68% on fresh weight basis at 14 days of cultivation.

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Resistance Degree of Radish Cultivars to Fusarium oxysporum f. sp. raphani according to Several Conditions (발병조건에 따른 무 품종들의 시들음병에 대한 저항성 차이)

  • Baik, Song-Yi;Jang, Kyoung-Soo;Choi, Yong-Ho;Kim, Jin-Cheol;Choi, Gyung-Ja
    • Horticultural Science & Technology
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    • v.29 no.1
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    • pp.48-52
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    • 2011
  • This study was conducted to establish the efficient screening system for resistant radish to Fusarium oxysporum f. sp. raphani. Five radish cultivars ('Myoungsan', 'Chungdu', 'Jangsaeng', 'Hannongyeorm', and 'Chungsukungjung') showing different degree of resistance to the fungus were selected. And the development of Fusarium wilt of the cultivars according to several conditions such as root wounding, dipping period of roots in spore suspension, inoculum concentration, and incubation temperature to develop the disease was tested. In distinguishing the resistance degree of the radish cultivars to the disease, non-cut roots were more effective than cut roots. And occurrence of Fusarium wilt of the radish plants increased in the proportion to increase of root-dipping period and spore concentration of the fungus. Thus, optimum conditions to differentiate susceptible and resistant cultivars to the disease were root-dipping period of 0.5 hour and spore concentration of $1{\times}10^7\;conidia{\cdot}mL^{-1}$. Disease severity of Fusarium wilt on the cultivars was changed with incubation temperature and the radish seedlings incubated at $25^{\circ}C$ represented the most difference of resistance and susceptibility to Fusarium wilt. From the above results, we suggest that the efficient screening method for resistant radish to Fusarium oxysporum f. sp. raphani would be to dip non-cut roots of fourteen-day-old radish seedlings in spore suspension of $1{\times}10^7\;conidia{\cdot}mL^{-1}$ for 0.5 hour and to transplant the inoculated plants to plastic pots with fertilized soil, and then to incubate the radish plants at a temperature of $25^{\circ}C$ for development of Fusarium wilt.

Detection of Auxotrophic Mutants form Valsa ceratosperma, the Causal Fungus of Apple Canker (사과나무 부란병균(腐爛病菌) Valsa ceratosperma에서의 Auxotrophic Mutants의 검출(檢出))

  • Hong, Yeon Gyu;Uhm, Jae Youl
    • Current Research on Agriculture and Life Sciences
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    • v.5
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    • pp.119-126
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    • 1987
  • This study was conducted to elucidate the most appropriate method to obtain auxotrophic mutants from Valsa ceratosperma, the causal fungus of apple canker, which may be used as a gene marker in detecting the transfer of the factors of avirulent strains to virulent strains. Among the 3 kinds of synthetic media tested, each have two formula for minimal and complete, the medium which has been used in study of Endothia parasitica (E. P medium) was turned out to be most appropriate for the growth of V. ceratosperma. A medium for single colony formation from pycnidiospore of this fungus was developed by adding 0.5% L - sorbose to the E. P minimal medium. The period of incubation in dark for preventing the photoreactivation after U. V irradiation was estimated as about 60hrs at which most of the spores become binucleate. Largest number of putative auxotrophs were obtained at about 50second of irradiation to the spores smeared on the medium for single colony formation, at which the survival rate of spores was 5 to 6 percent. With these method developed in this experiment, 161 isolates of putative auxotrophs were detected among which the nutrient requirement for 10 isolates were determined. Five out of 10 mutants were still virulent to apple tree and all but one could not sporulate.

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A Study of the Images of General Supers and a Department Store in a Local City (지방도시에 입점하고 있는 종합슈퍼와 백화점에 대한 점포이미지 비교 분석)

  • Kim, Chang-Gon
    • Journal of Distribution Science
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    • v.10 no.6
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    • pp.17-26
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    • 2012
  • Suncheon is a city comprising a rural and urban area, where there are four types of large stores. Studies have shown that there are too many large stores serving the local population of just 300,000. However, geographically, Suncheon is located at a transportation hub that borders the cities of Gwangyang and Yeosu as well as the local counties of Boseong and Gurae. Residents of these areas can reach these shopping stores within an hour's drive. Thus, the managers of these four stores regard residents in these areas as their valued customers and endeavor to create a differentiated image among them. In this study, 13 different images were used to determine the public's opinions and feelings towards these stores and the differences were analyzed. The store images measured overall store impression, diversity of the product, the quality of products displayed at the store, accessibility, the atmosphere, service to the customers, and so on. These images are evaluated subjectively by each customer and are major factors in them deciding to revisit the stores. The 13 images are classified into five main categories and further classified into 13 sub-categories. Three kinds of factor images were extracted from the store images in the five main categories by factor analysis using SPSS Ver. 19. The first factor image was extracted from the images of convenience, atmosphere, and service in the main categories and is called a sub-service factor for the store in this study. Accessibility to the store was classified as a convenience image in the main category and was extracted as a common factor along with diversity and the price of goods. These differences are expected according to the store location, that is, the difference between stores located in a large city and those in a small local city, and depending on the nature of survey respondents. The result shows that there is a significant difference between the stores' images with regard to accessibility, the price of products, brand image, and lighting/sound image. This study has the following limitations. First, the survey sample was restricted to residents of a small local city that includes rural and urban populations. The differences between the store images regarding traffic and accessibility are factored by store location, whether they are located within a large or a small city as well as the economic situation of these cities. Second, only the customers of large-scale stores were included in the survey as respondents. Relatively large traditional markets are held every five days in local cities and there is competition between large-scale stores and traditional markets with regard to diversity and the price of goods. It could be expected that customers in large-scale stores and customers in traditional markets would hold different store images. In future studies, images of stores in large cities should be compared with the images of stores located in small local cities. In addition, customer behavior when buying goods in large-scale stores should be compared with their behavior when buying goods in traditional markets.

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Optimal Designs of Urban Watershed Boundary and Sewer Networks to Reduce Peak Outflows (첨두유출량 저감을 위한 도시유역 경계 및 우수관망 최적 설계)

  • Lee, Jung-Ho;Jun, Hwan-Don;Kim, Joong-Hoon
    • Journal of the Korean Society of Hazard Mitigation
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    • v.11 no.2
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    • pp.157-161
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    • 2011
  • Although many researches have been carried out concerning the watershed division in natural areas, it has not been researched for the urban watershed division. If the boundary between two urban areas is indistinct because no natural distinction or no administrative division is between the areas, the boundary between the urban areas that have the different outlets (multi-outlet urban watershed) is determined by only designer of sewer system. The suggested urban watershed division model (UWDM) determines the watershed boundary to reduce simultaneously the peak outflows at the outlets of each watershed. Then, the UWDM determines the sewer network to reduce the peak outflow at outlet by determining the pipe connecting directions between the manholes that have the multi-possible pipe connecting directions. In the UWDM, because the modification of the sewer network changes the superposition effect of the runoff hydrographs in sewer pipes, the optimal sewer layout can reduce the peak outflow at outlet, as much as the superposition effects of the hydrographs are reduced. Therefore, the UWDM can optimize the watershed distinction in multi-outlet urban watershed by determining the connecting directions of the boundary-manholes using the genetic algorithm. The suggested model was applied to a multi-outlet urban watershed of 50.3ha, Seoul, Korea, and the watershed division of this model, the peak outflows at two outlets were decreased by approximately 15% for the design rainfall.

Effects of Overwintering Disease Prevention in Korean Ginseng(Panax ginseng C.A. Meyer) by an Agronomical Control Measure in Paddy Field (논 재배 인삼의 월동병해 발생경감을 위한 경종적 처리효과)

  • Seong, Bong-Jae;Kim, Sun-Ick;Lee, Ka-Soon;Kim, Hyun-Ho;Kang, Yun Kyu;Cho, Jin-Woong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.2
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    • pp.152-158
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    • 2019
  • This study was conducted to develop and prove the effects of an agronomical pest control measure on ginseng cultivated by direct seeding in paddy field, and the results obtained are as follows. Decomposition of ginseng in field during overwintering was due to gray mold rot caused by Botrytis cinerea, which occurred in October or November of 2016 and intensified in February and March the following year. The occurrence rate of gray mold rot based on shading materials was 6.5%, 16.8%, and 29.5% with light-proof paper, PE shade net, and rice straw shade, respectively. The initial infection occurred in the leaves prior to wintering and secondary infection occurred in the stems after wintering. The rate of screrotium formation by gray mold in the above-ground parts of ginseng tended to increase: 26.6% on October 20, 33.7% in November 20, and 41.8% on December 20. The force needed to remove the leaves and stems from withered ginseng was 0.2, 0.94, 2.5, and 5 kg for 1-, 2-, 3-, and 4- and 5-year holds; the force required was 1 kg after wintering, making it relatively easy to remove. The disease incidence rate after the removal of leaves and stems was 2.5%, 1.2%, and 2.2% in 4-, 5-, and 6-year-old plants, respectively, and a disease high incidence rate of 8.8%, 13.0%, and 18.2%, respectively, was seen when the leaves and stems were not removed. In both transplanting and direct seeding, the miss-planted rate decreased and the germination rate increased when shading material was removed and the surface of ridge was covered with soil or vinyl.

The Quantity and Pattern of Leaf Fall and Nitrogen Resorption Strategy by Leaf-litter in the Gwangneung Natural Broadleaved Forest (광릉숲 천연활엽수림의 수종별 낙엽 현상과 질소 재전류 특성)

  • Kwon, Boram;Kim, Hyunseok;Yi, Myong Jong
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.3
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    • pp.208-220
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    • 2019
  • The seasonality of leaf fall has important implications for understanding the response of trees' phenology to climate change. In this study, we quantified the leaf fall pattern with a model to estimate the timing and speed of leaf litter according to species and considered the nutrient use strategy of canopy species. In the autumns of 2015 and 2016, leaf litter was collected periodically using 36 litter-traps from the deciduous forests in Gwangneung and sorted by species. The seasonal leaf fall pattern was estimated using the non-linear regression model of Dixon. Additionally, the resorption rate was calculated by analyzing the nitrogen concentration of the leaf litter at each collection time. The leaf litter generally began in early October and ended in mid-November depending on the species. At the peak time (T50) of leaf fall, on average, Carpinus laxiflora was first, and Quercus serrata was last. The rate of leaf fall was fastest (18.6 days) for Sorbus alnifolia in 2016 and slowest (40.8 days) for C. cordata in 2015. The nitrogen resorption rates at T50 were 0.45% for Q. serrata and 0.48% for C. laxiflora, and the resorption rate in 2015 with less precipitation was higher than in 2016. Since falling of leaf litter is affected by environmental factors such as temperature, precipitation, photoperiod, and $CO_2$ during the period attached foliage, the leaf fall pattern and nitrogen resorption differed year by year depending on the species. If we quantify the fall phenomena of deciduous trees and analyze them according to various conditions, we can predict whether the changes in leaf fall timing and speed due to climate change will prolong or shorten the growth period of trees. In addition, it may be possible to consider how this affects their nutrient use strategy.