• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.110-114
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• 2010

Highest increase of biomass was observed when tissue-cultured potato (Solanum tuberosum L. cv. Chubaek) shoots were cultured in a liquid medium containing 1/3 MS solution in a 18 L bioreactor, as compared to 1/4 and 1/2 MS solution. The medium containing 1/4 MS solution showed higher increase of shoot biomass than one containing 1/2 MS solution. Potato microtubers were formed when the medium was exchanged with the medium for microtuber formation and incubated under dark condition. The microtubers were observed first at some axillary buds one week after incubation under dark condition and then at most of the axillary buds by the end of 3 weeks. The 1.5 MS liquid medium and $20^{\circ}C$ were optimal conditions. By the end of 6 weeks, more 1,000 microtubers were formed in the 18 L bioreactor. Then, greened microtubers were harvested after one week culture under light condition.

• The Korean Journal of Mycology
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• v.31 no.2
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• pp.94-97
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• 2003

In order to shorten cultivation periods of Agrocybe cylindracea, liquid spawn was used in place of sawdust spawn. Optimum culture condition of liquid spawn was PSB (potato extract sugar broth) medium, $25^{\circ}C$ temperature and pH 7.0. And, optimum inoculum volume was $10{\sim}15ml$. Two grams per liter of dry weight of the fungal mycelia were obtained with 10l of culture bottle with liquid spawn after 10 days. In 850 ml-bottle of sawdust media, cultivation period using liquid spawn (30 days) was five days shorter compared with the cultivation period in sawdust spawn (35 days). Also, complete growth of sawdust media was increased to 92% (liquid spawn) from 75% (sawdust spawn) and yield of fruiting bodies was increased to about 10%.

• Korean Journal of Plant Resources
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• v.12 no.4
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• pp.253-259
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• 1999

In this study, three simple methods were established to confirm the transgenic potato plants. The leaf disc was used in the first method. After leaf discs of transgenic and non-transgenic potato were transfered into the liquid MS medium with bialaphos 5mg/l, 25 days, the chlorosis occurred in the non-transgenic leaf discs while it could not find in the transgenic leaf discs, In the second method, shoot tips of potato were transferred into MS medium supplemented with 0.5mg/l bialaphos and 0.6% agar. After 7-10 days, a lot of roots developed from the transgenic shoot tip, but the non-transgenic shoot tip was dead. The third method was using chlorophyll contents. Leaf discs were transferred into the liquid MS medium with bialaphos 0.5 mg/l. After 15 days, the content of chlorophyll A in transgenic plant was at least 2.5 times higher than in non-transgenic plant. In addition, the PAT enzyme activity were detected in the transgenic potato, but not detected in normal potato.

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.1-6
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• 2009

A study was conducted to investigate in vitro micro propagation of four potato cultivars of Daese, Jasim, Chubaek and Haryeng in MS medium and PM medium (a medium containing half concentration of $NH_4NO_3$ and $KNO_3$, two fold concentration of $CaCl_2$ as compared to MS medium). During $1{\sim}2$ weeks of culture, Daese and Jasim showed better shoot elongation on the solid MS medium than Chubaek and Haryeng whereas Chubaek and Haryeng did better shoot elongation on the solid PM medium. But no difference was observed after 4 weeks of culture. As compared to shoot elongation on the solid medium, it was delayed at early stage of culture in the liquid medium without shaking. Shoot formation ratio of potato (above 4 cm of shoot length) began to increase significantly after 1 week of culture and kept on increasing until 4 weeks. The four cultivars showed the different patterns of shoot growth in bioreactor. The PM medium with a quarter salt strength was effective for the regeneration of axillary buds as well as shoot elongation of Jasim and Chubaek. Daese showed vigorous regeneration of axillary buds in the PM medium with a half salt strength. On the other hand, Haryeng showed slower growth than the other three cultivars.

• Journal of Bio-Environment Control
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• v.23 no.2
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• pp.144-147
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• 2014

It was intended to closely examine an effect that a change in the concentration of culture medium had on the potato(Solanum tuberosum L.) plantlet growth in the microponic system so as to mass-produce the virus-free plant of new variety 'Saebong' for potato processing. The adjusted concentration of potato culture medium was 0.2, 0.6, 1.0, 1.4, 1.8, and $14.0dS{\cdot}m^{-1}$. And potato seedling was cut into pieces of 1.5 cm in length, which included 2 growth points and leaves. And each was explanted in glass vial of 50 mL. And experiments were carried out twice for 18 days or 21days. Culture medium of 2ml was put in the container respectively. And 1 mL was added after 10 days. And in terms of cultivation environment, the experiment was carried out at the day length of 16 hours at the temperature of $23{\pm}1^{\circ}C$ under the white LED light of $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. The concentration of culture medium in the experiment I was EC 0.2, 1.0, $14dS{\cdot}m^{-1}$ and was adjusted to 0.6, 1.0, 1.4, $1.8dS{\cdot}m^{-1}$ in the experiment II. The results showed that the survival rate of plantlet was 90% at $0.2dS^2m^{-1}$, 100% at $0.6dS^2m^{-1}$, 100% at $1.0dS^2m^{-1}$. 0% at $1.4dS{\cdot}m^{-1}$, 0% at $1.8dS{\cdot}m^{-1}$. and 0% at $14.0dS{\cdot}m^{-1}$ after 7 days. With regard to the explanted potato seedling, in case of the treatment where the electrical conductivity of culture medium was adjusted to $1.0dS{\cdot}m^{-1}$, root developed 2 days after transplantation. And the plantlet vigorously grew into strong plant that had 7 leaves, length of 5cm, and fresh weight of 0.5 g after 18 days. In case of the treatment where the concentration of culture medium was adjusted to $0.6dS{\cdot}m^{-1}$, the root plantlets developed 4 days after transplantation. And those grew into plant that had 7 leaves and fresh weight of 0.2 g after 21 days. Therefore, we found that it is effective to control potato culture medium by adjusting its electrical conductivity to $0.6{\sim}1.0dS{\cdot}m^{-1}$ for the mass production of virus-free potato seedling in the microponic system.

• The Korean Journal of Mycology
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• v.33 no.1
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• pp.22-29
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• 2005

The main objectives of this research were to study the cultural and nutritional characteristics of Tricholoma matsutake and to establish its liquid culture system. The optimum growth of T. matsutake was observed in HA and TMM agar media. Similarly highest growth was observed in PDB and TMM liquid media. The optimal temperature for the mycelial growth was $25^{\circ}C$. The most suitable carbon source was dextrin among 12 different carbon sources tested. Yeast extract and peptone were best nitrogen sources among 17 different sources tested. The optimum mineral salts were $Fe_{2}(SO_{4})_{3}{\cdot}H_{2}O$ and KCl among 9 different sources tested. Shaking culture gave higher mycelial growth compared to stationary culture. Similarly, optimum medium amount for shaking culture was 100 ml per 250 ml flask. The highest mycelial growth was obtained when $5{\sim}7$ mycelial discs were inoculated in 100 ml of medium and incubated for $8{\sim}9$ weeks, respectively. The highest proportion of mycelial growth was observed at 40 : 1 ratio of medium to inoculum volume in 8 l air-lift fermenter.

• The Korean Journal of Mycology
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• v.43 no.3
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• pp.165-173
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• 2015

Mattirolomyces terfezioides is a type of sweet truffles that predominantly form ectomycorrhizae with Robinia pseudoacacia. It is also worthy of artificial cultivation. This is the first report on characteristics of mycelial growth and enzyme activities of M. terfezioides collected from R. pseudoacacia forest in Korea. M. terfezioides showed the highest mycelial growth when cultured on potato dextrose agar (PDA) at $30^{\circ}C$ or in modified Melin-Norkran's liquid medium (pH 8.0). The biomass of M. terfezioides was higher in liquid medium containing nitrate-nitrogen than ammonium-nitrogen by 1.8 fold. The mycelia of M. terfezioides showed both carboxymethylcellulase and laccase activities on solid media for enzyme screening.

• Korean Journal Plant Pathology
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• v.11 no.2
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• pp.165-172
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• 1995

벼 잎집무늬마름병균(Rhizoctonia solani)의 균핵에서 분리한 길항세균 SJ-2를 대치 배양 방법에 의해 벼도열병균(Pyricularia oryzae)외 6종의 식물 병원균에 대한 억제 효과를 조사하였다. 형태 및 생리적 특성을 조사한 결과, 이 균은 Bacillus subtilis로 동정되었다. 이 균은 AM 1(antibiotic medium 1)액체 배지에서 항균 물질을 분비하였으며, 배양 2일째 가장 항균 활성이 높았다. Butyl alcohol로 배양액에서 항균 물질을 조추출하여 100$\mu\textrm{g}$/ml의 농도로 조제한 감자 한천 배지에서 P. oryzae의 15개균에 대한 생육을 조사한 결과 P. oryzae, R. solani, Cochliobolus sativus에 대해서는 100% 생장을 억제하였으며, C. miyabeanus, Alternaria alternata, Botrytis cinerea, Cladosporium fulvum, Colletotrichum gloeosporioides, Fusarium moniliforme, F. oxysporum에는 80% 이상의 억제 효과를 보인 반면 난균강에 속하는 병원균에 대한 효과는 낮게 나타났다. 활성물질을 분리 정제하여 동정한 결과 B. subtilis가 분비하는 항균 물질로 알려진 polypeptides계의 iturins로 밝혀졌다. 이러한 항균 물질은 벼 도열병균(P. oryzae)의 포자 발아 및 발아관의 팽대(swelling)를 야기했으며, 벼 잎집무늬마름병균(R. solani)에는 균사의 용균(lysis)현상을 일으켰다.

• Korean Journal of Plant Tissue Culture
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• v.28 no.4
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• pp.215-219
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• 2001

This study was conducted to investigate the effect of culture media and environment on PLB proliferation by using PLBs produced from leaf segments excised from shoot of Phalanopsis flower stalk. The fresh weight of PLBs propagated was higher in MS medium than in NDM (New Dogashima medium) or VW, but the condition of PLB was better in NDM medium. Natural additives of Coconut water, potato and apple were absolutely required for the PLB propagation. PLB propagation was better in solid medium than in liquid medium including cotton as support. Optimal sucrose concentration for proliferation was 10 g/L. PLB proliferation was very effective condition 14.3 $\mu$mol.s$^{-1}$ m$^{-2}$ in PPFD and $25^{\circ}C$.

• The Korean Journal of Mycology
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• v.36 no.1
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• pp.16-21
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• 2008

This study was carried out to obtain the basic data for the mycelial growth conditions of cauliflower mushroom, Sparassis crispa. Twenty-one isolates were collected from domestic and abroad. The optimal temperature and pH for the mycelial growth of these isolates were $23^{\circ}C$ and pH 6.0, respectively. The mycelial growth was the best in the HBA medium, but very poor in the Lilly medium. However no mycelial growth in the CzapekDox medium. The utilization of carbon source was the best with fructose, and that of nitrogen source was the best with glutamine when compared to other tested sources. The selected isolate ASI150010 produced the highest mycelial weight in liquid culture containing soybean mill ($15\;g/{\ell}$) and potato ($200\;g/{\ell}$) extract. And uncleaned rice, wheat and barley were found to be good substrates for the mycelial growth S. crispa.