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Cultural Characteristics of Cauliflower Mushroom, Sparassis crispa

꽃송이버섯(Sparassis crispa)의 배양적 특성

  • Cheong, Jong-Chun (Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Park, Jeong-Sik (Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Hong, In-Pyo (Applied Sericulture and Apiculture Division, NIAST, RDA) ;
  • Seok, Soon-Ja (Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Jhune, Chang-Sung (Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Lee, Chan-Jung (Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration)
  • 정종천 (농촌진흥청 농업과학기술원 농업환경부 응용미생물과) ;
  • 박정식 (농촌진흥청 농업과학기술원 농업환경부 응용미생물과) ;
  • 홍인표 (농업생물부 잠사양봉소재과) ;
  • 석순자 (농촌진흥청 농업과학기술원 농업환경부 응용미생물과) ;
  • 전창성 (농촌진흥청 농업과학기술원 농업환경부 응용미생물과) ;
  • 이찬중 (농촌진흥청 농업과학기술원 농업환경부 응용미생물과)
  • Published : 2008.06.30

Abstract

This study was carried out to obtain the basic data for the mycelial growth conditions of cauliflower mushroom, Sparassis crispa. Twenty-one isolates were collected from domestic and abroad. The optimal temperature and pH for the mycelial growth of these isolates were $23^{\circ}C$ and pH 6.0, respectively. The mycelial growth was the best in the HBA medium, but very poor in the Lilly medium. However no mycelial growth in the CzapekDox medium. The utilization of carbon source was the best with fructose, and that of nitrogen source was the best with glutamine when compared to other tested sources. The selected isolate ASI150010 produced the highest mycelial weight in liquid culture containing soybean mill ($15\;g/{\ell}$) and potato ($200\;g/{\ell}$) extract. And uncleaned rice, wheat and barley were found to be good substrates for the mycelial growth S. crispa.

본 시험은 국내외에서 수집한 꽃송이버섯균 8균주에 대하여 균의 배양적 특성 및 균사체 대량배양 조건을 밝히고자 몇 가지 실험을 하였다. 꽃송이버섯균의 고체배양시 HBA(벌꿀바나나추출배지)에서 $53.0{\pm}5.3mm{\cdot}15$$^{-1}$로 균사생장이 빨랐다. 최적배양온도는 $23^{\circ}C$이었으며 pH는 6.0이 었고, 탄소원으로 fructose, 질소원은 glutamine을 잘 이용하였다. 꽃송이버섯균의 균사체 대량배양을 위한 균주로는 통기식 액체배양시 균사체 생산량이 많은 ASI150010을 선발하였다. 액체배양용 추출배지 재료인 대두박추출배지 ($15\;g{\cdot}{\ell}^{-1}$)에서 30일 동안의 균사량은 1.85g으로 감자추출배지 ($200\;g{\cdot}{\ell}^{-1}$)의 1.05g보다 1.7배가 많았다. 곡물을 이용한 꽃송이버섯 균사체 배양시 수분조절 방법으로 현미는 1일간 침수 처리에서 균사체 생장이 빨랐으며, 밀과 보리는 삶는 방법이 좋았다. 그리고 밀은 보리보다 균배양이 빨라서 좋았다.

Keywords

References

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