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Mycorrhizae, mushrooms, and research trends in Korea (균근과 버섯 그리고 국내 연구동향)

  • An, Gi-Hong;Cho, Jae-Han;Han, Jae-Gu
    • Journal of Mushroom
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    • v.18 no.1
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    • pp.1-9
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    • 2020
  • Mycorrhiza refers to the association between a plant and a fungus colonizing the cortical tissue of the plant's roots during periods of active plant growth. The benefits afforded by plants from mycorrhizal symbioses can be characterized either agronomically, based on increased growth and yield, or ecologically, based on improved fitness (i.e., reproductive ability). In either case, the benefit accrues primarily because mycorrhizal fungi form a critical linkage between plant roots and the soil. The soilborne or extramatrical hyphae take up nutrients from the soil solution and transport them to the root. This mycorrhizae-mediated mechanism increases the effective absorptive surface area of the plant. There are seven major types of mycorrhizae along with mycoheterotrophy: endomycorrhizae (arbuscular mycorrhizae, AM), ectomycorrhizae (EM), ectendomycorrhizae, monotropoid, arbutoid, orchid, and ericoid. Endomycorrhizal fungi form arbuscules or highly branched structures within root cortical cells, giving rise to arbuscular mycorrhiza, which may produce extensive extramatrical hyphae and significantly increase phosphorus inflow rates in the plants they colonize. Ectomycorrhizal fungi may produce large quantities of hyphae on the root and in the soil; these hyphae play a role in absorption and translocation of inorganic nutrients and water, and also release nutrients from litter layers by producing enzymes involved in mineralization of organic matters. Over 4,000 fungal species, primarily belonging to Basidiomycotina and to a lesser extent Ascomycotina, are able to form ectomycorrhizae. Many of these fungi produce various mushrooms on the forest floor that are traded at a high price. In this paper, we discuss the benefits, nutrient cycles, and artificial cultivation of mycorrhizae in Korea.

Comparison of Estradiol Results according to Manufacturer (제조회사에 따른 Estradiol 결과값의 비교)

  • Kim, Dae-Woon;Shin, Hee-Jung;You, Tae-Min;Noh, Gyeong-Woon;Kim, Hyun-Joo
    • The Korean Journal of Nuclear Medicine Technology
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    • v.15 no.1
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    • pp.101-105
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    • 2011
  • Purpose: Estradiol in the menstrual cycle and ovulation induction as an important test of currently national nuclear medicine laboratory in the normal patients and patients with infertility tests are being performed. For the diagnosis of menopause is an important test with follicle stimulating hormone (FSH) and Luteinizing hormone (LH). Currently participating in external quality control of the nation's hospitals that is 54 percent of 37 hospitals, 20 hospitals have been using A's reagent. The kit's test results are highly different from other kit comes with the test results of specimens have been found. And for the phenomenon is to study the problem. Materials and Methods: Estraiol test were referred to our hospital which results of samples as more than 100pg/ml 75 specimens measured by radioimmunoassay(RIA) test with company A company B company C company D Kit, Chemiluminescent assay (CMIA) to examine and compare to the results from april to August in 2010. Results: Kit for each manufacturing company as measured by the results obtained using the average value of the correlation coefficient (R2) and A company 0.8906 B 0.9527 C 0.9547 and D company correlation coefficient of 0.873 showed a good correlation that measuring the results of A company high concentrations when Company B Company C Company D with CMIA test concentrations measured low results that the two cases were discovered specimens. Conclusion: Most of the test results of 75 samples came up with a similar trend, but two cases were reported in the patients very differently. A company result reported higher than 700 pg/ml, while the rest of other test results report was approximately 10 pg/ml. The common point of two samples more than 50 years patients are estimated to be diagnosed with cancer in postmenopausal patients receiving treatment and levels of FSH were found to be greater than 50 mIU/ml. Did not identify the exact cause. I suggest if you are using A company kit that need to again check when Estradiol result and follicle stimulating hormone results is higher.

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The Evaluation of Usefulness of Pixelated Breast-Specific Gamma Imaging in Thyroid scan (Pixelated Breast-Specific Gamma Imaging(BSGI) 감마 카메라를 이용한 갑상선 검사의 유용성 평가)

  • Jung, Eun-Mi;Seong, Ji-Hye;Yoo, Hee-Jae
    • The Korean Journal of Nuclear Medicine Technology
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    • v.15 no.1
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    • pp.90-93
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    • 2011
  • Purpose: A Pixelated BSGI gamma camera has features to enhance resolution and sensitivity and minimize the distance between detector and organs by narrow FOV. Therefore, it is known as useful device to examine small organs such as thyroid, parathyroid and gall bladder. In general, when we would like to enlarge the size of images and obtain high resolution images by gamma camera in nuclear medicine study, we use pinhole collimator. The purpose of this study is to evaluate the usefulness of Pixelated BSGI gamma camera and to compare to it using pinhole collimator in thyroid scan which is a study of typical small organs. Materials and methods: (1) The evaluation of sensitivity and spatial resolution: We measured sensitivity and spatial resolution of Pixelated BSGI with LEHR collimator and Infinia gamma camera with pinhole collimator. The sensitivity was measured by point source sensitivity test recommended by IAEA. We acquired images considering dead time in BSGI gamma camera for 100 seconds and used $^{99m}TcO4-\;400{\mu}Ci$ line source. (2) The evaluation of thyroid phantom: The thyroid phantom was filled with $^{99m}TcO4-$. After set 300 sec or 100 kcts stop conditions, we acquired images from both pixelated BSGI gamma camera and Infinia gamma camera with LEHR collimator. And we performed all thyroid studies in the same way as current AMC's procedure. Results: (1) the result of sensitivity: As a result, the sensitivity and spatial resolution of pixelated BSGI gamma camera were better than Infinia's. The sensitivities of pixelated BSGI and Infinia gamma camera were $290cps/{\mu}Ci$ and $350cps/{\mu}Ci$ respectively. So, the sensitivity of pixelated BSGI was 1.2 times higher than Infinia's (2) the result of thyroid phantom: Consequently, we confirmed that images of Pixelated BSGI gamma camera were more distinguishable between hot and cold spot compared with Infinia gamma camera. Conclusion: A pixelated BSGI gamma camera is able to shorten the acquisition time. Furthermore, the patients are exposed to radiation less than before by reducing amount of radiopharmaceutical doses. Shortening scan time makes images better by minimizing patient's breath and motion. And also, the distance between organ and detector is minimized because detector of pixelated BSGI gamma camera is small and possible to rotate. When patient cannot move at all, it is useful since device is feasible to move itself. However, although a pixelated BSGI gamma camera has these advantages, the effect of dead time occurs over 2000 cts/s since it was produced only for breast scan. So, there were low concentrations in organ. Therefore, we should consider that it needs to take tests to adjust acquisition time and amount of radiopharmaceutical doses in thyroid scan case with a pixelated BSGI gamma camera.

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A Comparative Analysis of Standard Uptake Value Using the Recovery Coefficient Before and After Correcting Partial Volume Effect (부분 체적 효과에서 회복 계수를 이용한 보정 전과 후 SUV의 비교 분석)

  • Ko, Hyun-Soo;Park, Soon-Ki;Choi, Jae-Min;Kim, Jung-Sun;Jung, Woo-Young
    • The Korean Journal of Nuclear Medicine Technology
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    • v.15 no.1
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    • pp.10-16
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    • 2011
  • Purpose: The partial volume effect occurs because of limit of the spatial resolution. It makes partial loss of intensity and causes SUV to be lower than it should actually be. So the purpose of this study is to calculate recovery coefficient for correcting PVE from phantom study and to compare before and after SUV correction applying to PET/CT examination. Materials and Methods: The flangeless Esser PET phantom consisting of four hot cylinders was used for this study. All of the hot cylinders were filled with FDG solution of 20.72 MBq per 1000 ml, and the phantom background was filled with FDG solution of different concentrations (33.30, 22.20, 16.65 MBq per 6440 ml) to yield H/B ratios of around 4:1, 6:1 and 8:1. Using the Biograph Truepoint 40(SIEMENS, Germany), we applied recovery coefficient method to 30 patients who were diagnosed with lung cancer after PET/CT exam. And then we analyzed and compared SUV before and after correcting partial volume effect. Results: The smaller the diameter of hot cylinder becomes, the more recovery coefficient decreased. When we applied recovery coefficient to clinical patients and compared SUV before and after correcting PVE, before the correction all lesions gave an average max SUV of 7.83. And after the correction, the average max SUV increases to 10.31. The differences in the max SUV between before and after correction were analyzed by paired t test. As a result, there were statistically significant differences (t=7.21, p=0.000). Conclusion: The SUV for quantification should be measured precisely to give consistent information of tumor uptake. But PVE is one of factors that causes SUV to be lower and to be underestimated. We can correct this PVE and calculate corrected SUV using the recovery coefficient from phantom study. And if we apply this correction method to clinical patients, we can finally assess and provide quantitative analysis more accurately.

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Studies on the grassland Development in the Forest V. Effect of sharing degrees on the early growth characteristics and winter survival of main grasses (임간초지 개발에 관한 연구 V. 차광정도가 주요 초목의 초기생육과 월동에 미치는 영향)

  • Park, M.S.;Seo, S.;Han, Y.C.;Lee, J.Y.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.6 no.1
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    • pp.38-43
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    • 1986
  • This field experiment was conducted to determine the effects of shading degrees (0 : full sunlight, 25, 50 and 75%) on the early growth characteristics and winter survival of grasses. For the test different artificial shading houses were established and pasture species used were orchardgrass, timothy and perennial ryegrass. The experiment was performed at Livestock Experiment Station in Suweon, and sowing date was Aug. 25, 1984. The results are summarized as follows : 1. Generally, root length, new root length, number of new root and tillers of grasses were increased at 0% (full sunlight) of shading, followed by 25, 50 and 75% of shading degrees, in that order. Plant length and leaf elongation, however were increased at 50% of shading, followed by 75, 25 and 0%. 2. The percentage of winter killing of grasses was significantly (P<0.05) increased as the shading degrees increased. Also the percentages of winter killing were 10.1% in orchardgrass, 9.5% in timothy and 14.2% in perennial ryegrass, respectively. 3. A significant negative correlations were observed in the relationship between winter killing and tillers, new root length and number of new root of grasses. However, there were positive correlations between winter killing and plant length or leaf elongation under shading conditions. 4. As the shading degrees are increased by 0, 25, 50 and 75%, the NAR of grasses was tended to be decrease to 10.98, 11.52, 9.81 and 6.12 g/$m^2$/day, in that order. Also there was a negative correlation (r=-0.5943*) between winter killing and NAR of grasses, and NAR of orchardgrass, timothy and perennial ryegrass were 8.58, 8.53 and 11.71 g/$m^2$/day, respectively.

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Development of Auto-aging System Built in Kimchi Refrigerator for Optimal Fermentation and Storage of Korean Cabbage Kimchi (배추김치의 최적발효 및 저장을 위한 김치냉장고의 자동숙성 시스템 개발)

  • Noh, Jeong-Sook;Seo, Hyun-Ju;Oh, Jung-Hwan;Lee, Myung-Ju;Kim, Myung-Hee;Cheigh, Hong-Sik;Song, Yeong-Ok
    • Korean Journal of Food Science and Technology
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    • v.39 no.4
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    • pp.432-437
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    • 2007
  • In this study, we examined the conditions for an auto-aging system placed within a kimchi refrigerator for optimal fermentation, and to prolong the storage time of kimchi. Various characteristics of kimchi fermented at different temperatures ($5-23^{\circ}C$) were compared. We observed that the higher the fermentation temperature, the less desirable the overall acceptability of the product. To establish the time point in which to convert the fermentation mode to the storage mode, kimchi was stored at $-1^{\circ}C$ for 1 week once it reached the designated acidity (0.4, 0.6, and 0.8%). The results indicated that the lower the kimchi acidity, the higher the sensory score. The storage temperature of $-1^{\circ}C$ was not low enough to retard microorganism growth completely; however, the kimchi stored at $-2{\pm}0.5^{\circ}C$ became frozen. Accordingly, $15^{\circ}C$ and $-2{\pm}0.5^{\circ}C$ are suggested as the fermentation and storage temperatures for the kimchi refrigerator, respectively. A kimchi acidity of 0.4% can be used as an index for the time point to convert fermentation to storage. Subsequently, the time required for the fermentation course can be calculated based on this.

Development of Auto-aging System for the Kimchi Refrigerator for Optimal Fermentation and Storage of Dongchimi (동치미 최적발효 및 저장을 위한 김치냉장고의 자동숙성 시스템 개발)

  • Noh, Jeong-Sook;Kim, Jong-Hyun;Lee, Myung-Ju;Kim, Myung-Hee;Song, Yeong-Ok
    • Korean Journal of Food Science and Technology
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    • v.40 no.6
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    • pp.661-668
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    • 2008
  • This study was investigated to determine the conditions for an auto-aging system placed within a kimchi refrigerator for optimal fermentation, as well as the optimal conditions for the prolongation of dongchimi storage time. Various fermentation characteristics of dongchimi stored at different temperatures ($5-23^{\circ}C$) were compared. The pH and acidity of dongchimi associated with the highest levels of overall acceptability were $3.9{\pm}0.1$ and $0.25{\pm}0.05%$, respectively. In order to establish the time point for the conversion of fermentation to storage, dongchimi was fermented at $10^{\circ}C$ or $15^{\circ}C$ until the acidity reached the designated points (0.1, 0.2, and 0.3%), and was then stored at $-1^{\circ}C$ for 1 week. According to the sensory evaluation, the dongchimi acidity of 0.2% fermented at $10^{\circ}C$ evidenced the highest levels of overall acceptability after 1 week of storage among the samples. In conclusion, we suggest conditions of $10^{\circ}C$ and $-2.5{\pm}0.5^{\circ}C$ for the fermentation and storage temperature, respectively, and a dongchimi acidity of 0.2% as an index for the time point for the conversion of fermentation to storage in the kimchi refrigerator.

Effect of Horse Grazing Intensity on Changes of Sasa quelpaertenis Nakai Vegetation and Physiological Characteristics in Horses (말 방목 강도가 제주조릿대(Sasa quelpaertenis Nakai) 식생과 말의 생리적 변화에 미치는 영향)

  • Woo, Jae-Hoon;Park, Nam Geon;Kim, Nam-Young;Shin, Sang-Min;Shin, Moon-Cheol;Yoo, Ji-Hyun;Hwang, Won-uk;Yang, Byung-Chul;Kim, Hyun-Cheol
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.4
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    • pp.216-226
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    • 2019
  • This study was to investigate the effect of the horse grazing intensity on the vegetation of the S. quelpaertensis, and the physiological changes of the horse. This experiment was conducted at the community of the S. quelpaertensis (altitude of 550m) with the grazing intensity of 1.0AU(500kg), 1.5AU(750kg) and 2.0AU(1,000kg) at 30m × 30m for one week, and then the growth characteristics of the S. quelpaertensis, the changes of the vegetation, the damages on trees and the changes in the physiological characteristics of the horses was investigated and compared before and after the horse grazing. As the results, the 2.0AU grazing land showed that the culm number had the inverse correlation (r=-0.902, P<0.01) to the other lands and the species diversity index of lower-level vegetation showed the correlation (r=0.773, P<0.01) as increasing the grazing intensity.

Effects of Supercritical Fluid Marc Extracts from Actinidia polygama Max. on Inflammation and Atherosclerosis (개다래 초임계 박추출물이 염증 및 동맥경화에 미치는 영향)

  • Yu, Mi-Hee;Chae, In-Gyeong;Choi, Jun-Hyeok;Im, Hyo-Gwon;Choi, Hee-Don;Yang, Seun-Ah;Lee, Jin-Ho;Lee, In-Seon
    • Korean Journal of Food Science and Technology
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    • v.42 no.4
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    • pp.475-480
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    • 2010
  • The fruit of Actinidia polygama, Mock-chun-ryo in Korea, has been used as traditional medicine for abdominal pain, rheumatic arthritis, and stroke. In a previous study, the ethanol extract of A. polygama Max. showed antiinflammatory activity in RAW 264.7 cells. In this study, we investigated the anti-inflammatory and anti-atherosclerosis effects of supercritical fluid marc extracts from A. polygama Max. Anti-inflammatory extracts were produced from supercritical fluid extraction of the silver vine under the following conditions; pressure, 1,500-4,500 psi, temperature $35-55^{\circ}C$ and extraction time 1-2 hr. To evaluate the anti-inflammatory and anti-atherosclerotic effects of the extracts, we studied nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), and tumor necrosis factor-alpha (TNF-$\alpha$) levels in RAW 264.7 cells and MMP-9 activity in human aortic smooth muscle cells (HASMC). The Marc 11 extract inhibited the production of NO, $PGE_2$, and TNF-$\alpha$ by lipopolysaccharide in RAW 264.7 cells. Moreover, the marc 11 extract inhibited TNF-$\alpha$-induced MMP-9 activity in HASMC. These results indicate that the Marc 11 extract of A. polygama Max. has the potential for use as an anti-atherosclerosis agent.

Doctor's Perception and Referral Barriers toward Palliative Care for Advanced Cancer Patients (말기암환자의 완화의료에 대한 의사들의 인식과 완화의료 의뢰 시 장애요인)

  • Lee, Jae-Ri;Lee, Jung-Kwon;Hwang, Sun-Jin;Kim, Ji-Eun;Chung, Ji-In;Kim, Si-Young
    • Journal of Hospice and Palliative Care
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    • v.15 no.1
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    • pp.10-17
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    • 2012
  • Purpose: This study was conducted to identify the perception regarding palliative care among Korean doctors and referral barriers toward palliative care for terminal cancer patients. Methods: Between May and June 2010, 477 specialists mainly caring cancer patients using a web-based, self-administered questionnaire. Results: A total of 128 doctors (26.8%) responded. All respondents (100%) deemed palliative care a necessary service for terminal cancer patients. More than 80% of the respondents agreed to each of the following statements: all cancer centers should provide palliative care service (80.5%); all terminal cancer patients should receive concurrent palliative care along with anti-cancer therapies (89.1%) and caring for terminal cancer patients requires interdisciplinary approach (96.9). While more than 58% of the respondents were satisfied with their performance of physical and psychological symptoms management and emotional support provided by patient's family members, 64% of the responded answered that their general management of the end-of-life care was less than satisfactory. Doctors without prior experience in referring their patients to palliative care specialists accounted for 26.6% of the respondents. The most common barrier to hospice referral, cited by 47.7% of the respondents, was "refusal of patient or family member", followed by "lack of available palliative care resources" (46.1%). Conclusion: Although most doctors do recognize the importance of palliative care for advanced cancer patients, comprehensive and sufficient palliative medicine, including interdisciplinary cooperation and end-of-life care, has not been put into practice. Thus, more active palliative consultation or referral is needed for effective care of terminal cancer patients.