Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effects of Supercritical Fluid Marc Extracts from Actinidia polygama Max. on Inflammation and Atherosclerosis

개다래 초임계 박추출물이 염증 및 동맥경화에 미치는 영향

  • Yu, Mi-Hee (Department of Food Science and Technology, Keimyung University) ;
  • Chae, In-Gyeong (Department of Food Science and Technology, Keimyung University) ;
  • Choi, Jun-Hyeok (Korea Food Research Institute) ;
  • Im, Hyo-Gwon (Department of Food Science and Technology, Keimyung University) ;
  • Choi, Hee-Don (Korea Food Research Institute) ;
  • Yang, Seun-Ah (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, Jin-Ho (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, In-Seon (Department of Food Science and Technology, Keimyung University)
  • 유미희 (계명대학교 식품가공학) ;
  • 채인경 (계명대학교 식품가공학) ;
  • 최준혁 (한국식품연구원) ;
  • 임효권 (계명대학교 식품가공학) ;
  • 최희돈 (한국식품연구원) ;
  • 양선아 (계명대학교 전통미생물자원개발 및 산업화연구(TMR)센터) ;
  • 이진호 (계명대학교 전통미생물자원개발 및 산업화연구(TMR)센터) ;
  • 이인선 (계명대학교 식품가공학)
  • Received : 2010.02.16
  • Accepted : 2010.04.23
  • Published : 2010.08.31
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Abstract

The fruit of Actinidia polygama, Mock-chun-ryo in Korea, has been used as traditional medicine for abdominal pain, rheumatic arthritis, and stroke. In a previous study, the ethanol extract of A. polygama Max. showed antiinflammatory activity in RAW 264.7 cells. In this study, we investigated the anti-inflammatory and anti-atherosclerosis effects of supercritical fluid marc extracts from A. polygama Max. Anti-inflammatory extracts were produced from supercritical fluid extraction of the silver vine under the following conditions; pressure, 1,500-4,500 psi, temperature $35-55^{\circ}C$ and extraction time 1-2 hr. To evaluate the anti-inflammatory and anti-atherosclerotic effects of the extracts, we studied nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), and tumor necrosis factor-alpha (TNF-$\alpha$) levels in RAW 264.7 cells and MMP-9 activity in human aortic smooth muscle cells (HASMC). The Marc 11 extract inhibited the production of NO, $PGE_2$, and TNF-$\alpha$ by lipopolysaccharide in RAW 264.7 cells. Moreover, the marc 11 extract inhibited TNF-$\alpha$-induced MMP-9 activity in HASMC. These results indicate that the Marc 11 extract of A. polygama Max. has the potential for use as an anti-atherosclerosis agent.

개다래(Actinidia polyama Max.)는 복통, 류마티스 관절염, 뇌졸중 치료에 사용되었으며, 최근 소염, 진통, 통풍에 효과가 있다고 알려져 있으나 특유의 강한 향과 맛을 지니고 있어 기능성 식품이나 치료용 약물을 개발하고자 할 때 많은 제약이 따른다. 따라서 개다래를 초임계 추출하여 향과 맛을 개선하고, 본 연구에서는 초임계추출 후 남은 부산물을 이용하여 (박)추출물을 제조하고 항염증 및 항동맥경화 활성을 조사하였다. 개다래 주정 추출물 및 초임계 박 추출물의 NO 소거활성을 비교한 결과, 개다래박 11 추출물 10, $100\;{\mu}g/mL$의 농도에서 36.63, 79.58%의 NO 소거활성을 보여 주정 추출물(4.61, 19.00%)에 비해 훨씬 높은 NO 소거활성을 보였다. 또한 RAW 264.7 세포주에 개다래 초임계 박 11 추출물을 처리하고, LPS를 처리하여 염증을 유발한 후 염증인자인 $PGE_2$의 생성, 그리고 proinflammatory cytokines인 TNF-$\alpha$의 생성량을 측정한 결과, 개다래 박 11 추출물은 LPS로 유도된 염증 인자들을 효과적으로 감소시키는 것으로 나타났다. 또한 박 11 추출물의 MMP-9 활성에 미치는 영향을 알아보기 위해 zymography를 실시한 결과, 추출물 50, $100\;{\mu}g/mL$의 농도에서 MMP-9의 활성이 효과적으로 감소됨을 확인하였으며, MMP-9의 단백질 발현도 억제됨을 확인하였다. 결론적으로 개다래 박 11 추출물은 LPS로 유도된 염증 인자들을 효과적으로 감소시켰으며, TNF-$\alpha$에 의해 증가된 MMP-9의 활성을 억제하는 것으로 나타나 동맥경화를 비롯한 고혈압, 암, 당뇨, 관절염 등의 만성염증성 질환의 예방과 치료에 효과적으로 적용할 수 있을 것으로 생각된다.

Keywords

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