• Korean Journal of Acupuncture
• /
• v.27 no.1
• /
• pp.125-142
• /
• 2010

Objectives: The purpose of this study was to identify the effectiveness of neuronal activities for the acupuncture and laser acupuncture application. Methods: The subject were divided into 7 groups as control group without acupuncture, acupuncture treatment with tonify manipulation with the direction of channel at HT9, LR1(AT-A), acupuncture treatment with purge manipulation against the direction of channel at HT3, Kl10(AT-B), acupuncture treatment with tonify manipulation with the direction of channel at HT9, LR1 and purge manipulation against the direction of channel at HT3, KI10(AT-C), laser acupuncture treatment with red light 658 nm at HT9, LR1(LAT-A), laser acupuncture treatment with green light 532 nm at HT3, KI10(LAT-B), laser acupuncture treatment with red light 658 nm at HT9, LR1 and green light 532 nm at HT3, KI10(LAT-B). Antiapopotic effect of acupuncture was observed by Bax, Bcl-2 and cytochrome C. Neuroprotective effect of acupuncture was observed by cresyl violet and ChAT. Results: AT-A, AT-B, AT-C, LAT-A, LAT-B and LAT-C groups were significantly increased comparing the control groups in expression ChAT and in neuroprotective effect by cresyl violet. AT-A, AT-B, AT-C, LAT-A, LAT-B and LAT-C groups were significantly decreased comparing the control groups in expression Bax. AT-C, LAT-A, LAT-B and LAT-C groups were significantly increased comparing the control groups in expression Bcl-2. AT-A, AT-B, AT-C, LAT-A, LAT-B and LAT-C groups were significantly decreased comparing the control groups in Bax/Bcl-2 ratio. LAT-B and LAT-C groups were significantly decreased comparing the control groups in expression cytochrome C. Conclusions: The acupuncture with tonify and purge manipulation and laser acupuncture with red and green light could be effective for antiapopotic and neuroprotective effect in focal brain ischemia.

• International Journal of Oral Biology
• /
• v.34 no.3
• /
• pp.143-150
• /
• 2009

Amino acid transporters play important roles in supplying nutrients to cells. In our current study, we investigated the expression of LAT1 and measured the amino acid uptake in ameloblast cultures to further elucidate the roles of this transporter during the differentiation of these cells. RT-PCR, observations of cell morphology, Alizaline red-S staining, and uptake analyses were performed following the experimental induction of differentiation in the cultures. LAT1 mRNA was detectable and found to gradually increase over time whereas LAT2 mRNA was not evident in the ameloblast cultures. Transcripts of 4F2hc, a cofactor of LAT1 and LAT2, were also found to be expressed in ameloblast cultures and increase with time. Amelogenin mRNA was expressed in the early stage ameloblast cultures. L-leucine uptake was observed to increase over 14 days of growth in culture. Our data suggest that LAT1 has a key role in the differentiation of ameloblasts and in providing these cells with neutral amino acids, including several essential amino acids.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.24 no.4
• /
• pp.495-508
• /
• 2019

Tidal datums are key and basic information used in fields of navigation, coastal structures' design, maritime boundary delimitation and inundation warning. In Korea, the Approximate Lowest Low Water (ALLW) and the Approximate Highest High Water (AHHW) have been used as levels of tidal datums for depth, coastline and vertical clearances in hydrography and coastal engineering fields. However, recently the major maritime countries including USA, Australia and UK have adopted the Lowest Astronomical Tide (LAT) and the Highest Astronomical Tide (HAT) as the tidal datums. In this study, 1-hr interval 19-year sea level records (1999-2017) observed at 9 tidal observation stations along the west and south coasts of Korea were used to calculate LAT and HAT for each station using 1-minute interval 19-year tidal prediction data yielded through three tidal harmonic methods: 19 year vector average of tidal harmonic constants (Vector Average Method, VA), tidal harmonic analysis on 19 years of continuous data (19-year Method, 19Y) and tidal harmonic analysis on one year of data (1-year Method, 1Y). The calculated LAT and HAT values were quantitatively compared with the ALLW and AHHW values, respectively. The main causes of the difference between them were explored. In this study, we used the UTide, which is capable of conducting 19-year record tidal harmonic analysis and 19 year tidal prediction. Application of the three harmonic methods showed that there were relatively small differences (mostly less than ±1 cm) of the values of LAT and HAT calculated from the VA and 19Y methods, revealing that each method can be mutually and effectively used. In contrast, the standard deviations between LATs and HATs calculated from the 1Y and 19Y methods were 3~7 cm. The LAT (HAT) differences between the 1Y and 19Y methods range from -16.4 to 10.7 cm (-8.2 to 14.3 cm), which are relatively large compared to the LAT and HAT differences between the VA and 19Y methods. The LAT (HAT) values are, on average, 33.6 (46.2) cm lower (higher) than those of ALLW (AHHW) along the west and south coast of Korea. It was found that the S_a and N₂ tides significantly contribute to these differences. In the shallow water constituents dominated area, the M₄ and MS₄ tides also remarkably contribute to them. Differences between the LAT and the ALLW are larger than those between the HAT and the AHHW. The asymmetry occurs because the LAT and HAT are calculated from the amplitudes and phase-lags of 67 harmonic constituents whereas the ALLW and AHHW are based only on the amplitudes of the 4 major harmonic constituents.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.33 no.9
• /
• pp.1451-1456
• /
• 2004

Amino acid transporters play an important role in supplying nutrients to normal and cancer cells for cell proliferation. System L is a major transport system responsible for the $Na^+$ -independent, large neutral amino acids including several essential amino acids. L-type amino acid transporter 1 (LAT1), an isoform of system L amino acid transporter, is highly expressed presumably to support their continuous growth and proliferation in malignant tumors. In the present study, we have examined the correlation between the expressions of amino acid transporter LAT1 mRNA and its subunit 4F2hc mRNA and the amount of L-leucine transport in various human cancer cell lines. Northern blot analysis have revealed that the 26 human cancer cell lines expressed LAT1 mRNA and 4F2hc mRNA. There were the differences for the levels of LAT1 and 4F2hc mRNA expressions in the 26 human cancer cell lines. The 26 human cancer cell lines transported the L-[$^{14}C$]leucine into the cells via amino acid transporter. In the 26 human cancer cell lines, a linear relationship was observed between the expression of amino acid transporter LAT1 mRNA and the amount of L-leucine transport. Little relationship was observed between the expression of 4F2hc mRNA and the amount of L-leucine transport, but the statistical significance of difference was not detected. These results indicate that the 26 human cancer cell lines express LAT1 mRNA and 4F2hc mRNA and there is the correlation between the expression of amino acid transporter LAT1 mRNA and the amount of L-leucine transport. In addition, specific inhibition of LAT1 in cancer cells will be a new rationale for anti-cancer therapy.

• Asian-Australasian Journal of Animal Sciences
• /
• v.23 no.2
• /
• pp.272-278
• /
• 2010

In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human $y^{+}$LAT1 and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine $y^{+}$LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat $y^{+}$LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest $y^{+}$LAT1 mRNA abundance and the lung had the lowest $y^{+}$LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine $y^{+}$LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.

• Proceedings of the KIEE Conference
• /
• 1994.07a
• /
• pp.403-405
• /
• 1994

The dynamic behavior of circulating current cycloconverter and flywheel energy storage unit is the subject of this investigation. The system of this type can control of real and reactive power flow between flywheel-cycloconverter and power three phase network. In this paper, waveform level simulation indicates that power flow control of is possible and this system can be used to varible application.

• Journal of Acupuncture Research
• /
• v.31 no.2
• /
• pp.119-134
• /
• 2014

Objectives : The purpose of this study was to investigate the analgesic effects of filiform acupuncture and laser acupuncture at the points of $TE_3{\cdot}GB_{41}$ in neuropathic pain induced rats. Methods : Neuropathic pain in rats was induced by partial resection of the tibial and sural nerves. Three weeks after the neuropathic surgery, each of the experimental groups(AT, LAT, AT + LAT and LAT + AT) was injected at the $TE_3{\cdot}GB_{41}$ twice per week for three weeks. Results : 1. All of the experimental groups(AT, LAT, AT + LAT and LAT + AT) showed a significant decrease in the plantar withdrawal response of allodynia and the thermal allodynia as compared with the control group. During the early phase, the AT and AT + LAT groups have been marked as more significant than the LAT + AT and LAT groups. 2. The expression of c-Fos significantly decreased in the LAT and LAT + AT groups as compared with the control group. 3. The LAT + AT group showed a significant decrease in Bax as compared with the control group. In each experimental groups(AT, LAT, AT + LAT and LAT + AT), Bcl-2 increased and Bax/Bcl-2 ratio decreased as compared with the control group. 4. The LAT, AT + LAT and LAT + AT groups showed a significant increase in mGluR5 as compared with the control group. Conclusions : These results represented that the filiform acupuncture and laser acupuncture at the $TE_3{\cdot}GB_{41}$ exerted anti-apoptotic and neuroprotective effects on the model of neuropathic pain, thereby suggesting that they should be available for decreasing mechanical allodynia.

• Parasites, Hosts and Diseases
• /
• v.34 no.4
• /
• pp.233-238
• /
• 1996

The seroepidemiologic studies on antral-ToxopIasma antibody titers were carried out using ELISA and indirect latex agglutination test. Among 899 sera prepared from pregnant women, 39 cases (4.3%) revealed positive reaction and 218 sera from middle school students showed 4 positive reaction (1.8%) by ELISA. By LAT (newly established by National Veterinary Research Institute, Korea), the sera of 7 pregnant women (0.8%) showed positive reaction. When 80 sera showing ≥ 1 :8 by LAT were used for comparing the results obtained from LAT and Toxotest-MT (Eikon Chemical Co., Japans, 7 cases and 8 sera were positive, respectively. All of 11 sera of proven toxoplasmosis patients showed positive reaction in both tests. Overall proportion of agreement between LAT kit and Toxotest-MT was 0.94 (K-index : 0.632, p < 0.01), and LAT was considered to be useful for the screening of toxoplasmosis.

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.28 no.2
• /
• pp.101-108
• /
• 2016

In designing of the wind power facilities, the highest and lowest astronomical tides (HAT and LAT) are needed in terms of an international design tidal water levels. The AHHW and ALLW, however, have been used as the design tidal levels in Korea. The HAT and LAT in the Wido coastal sea should be estimated to satisfy the standard because the pilot wind power facilities will be located in the adjacent Wido coastal sea. In this study, the HAT and LAT are estimated using the 31-years hourly tidal elevation data of the Wido tidal gauging station and the nodal variation patterns of the major lunar components, such as $M_2$, $O_1$, and $K_1$, are analysed to check the expected long-term lunar cycle, i.e., 18.61-year's nodal variation patterns. The temporal amplitude variations of the $M_2$, $O_1$, and $K_1$ clearly show the 18.61-years periodic patterns in case of the no-nodal correction condition. In addition, the suggested HAT and LAT elevations, estimated as the upper and lower confidence limits of the yearly HAT and LAT elevations, show 40 cm greater than AHHW and 35 cm lower than ALLW, respectively.

• Archives of Pharmacal Research
• /
• v.28 no.4
• /
• pp.421-432
• /
• 2005

In order to understand the renal reabsorption mechanism of neutral amino acids via amino acid transporters, we have isolated human L-type amino acid transporter 2 (hLAT2) and human T-type amino acid transporter 1 (hTAT1) in human, then, we have examined and compared the gene structures, the functional characterizations and the localization in human kidney. Northern blot analysis showed that hLAT2 mRNA was expressed at high levels in the heart, brain, placenta, kidney, spleen, prostate, testis, ovary, lymph node and the fetal liver. The hTAT1 mRNA was detected at high levels in the heart, placenta, liver, skeletal muscle, kidney, pancreas, spleen, thymus and prostate. Immunohistochemical analysis on the human kidney revealed that the hLAT2 and hTAT1 proteins coexist in the basolateral membrane of the renal proximal tubules. The hLAT2 transports all neutral amino acids and hTAT1 transports aromatic amino acids. The basolateral location of the hLAT2 and hTAT1 proteins in the renal proximal tubule as well as the amino acid transport activity of hLAT2 and hTAT1 suggests that these transporters contribute to the renal reabsorption of neutral and aromatic amino acids in the basolateral domain of epithelial proximal tubule cells, respectively. Therefore, LAT2 and TAT1 play essential roles in the reabsorption of neutral amino acids from the epithelial cells to the blood stream in the kidney. Because LAT2 and TAT1 are essential to the efficient absorption of neutral amino acids from the kidney, their defects might be involved in the pathogenesis of disorders caused by a disruption in amino acid absorption such as blue diaper syndrome.