• Proceedings of the Korean Information Science Society Conference
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• 2006.10a
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• pp.203-208
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• 2006

본 논문에서는 MPEG-21 프레임워크를 Peer-to-Peer Network 상에서 구동시킬 때의 문제점을 해결하면서, P2P의 장점을 최대한 이용할 수 있도록 하는 P2P 방식의 디지털 권한 관리 시스템(P2PDRMEngine)을 설계한다. MPEG-21 프레임워크의 여러 구성 요소들 중 IPMP(Intellectual Property Management and Protection components), DI(Digital Item), Multimedia Contents 등의 구조에 몇 가지 요소를 추가하여 P2PDRMEngine과 MPEG-21 프레임워크가 서로 유기적인 동작을 할 수 있도록 설계하고 또한, DI를 생산, 배포, 사용할 때 사용자에게 편리성을 제공 해주는 기능도 고려한다. P2PDRMEngine은 기존의 MPEG-21 프레임워크의 수행에 지장을 주지 않으면서 보안적인 측면과 요소 간의 기능을 강화하는 데 초점을 맞추어 설계한 시스템이다.

• Parasites, Hosts and Diseases
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• v.39 no.3
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• pp.241-246
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• 2001

The antigenic domain of the major surface protein (Nc-p43) of Neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as GST fusion proteins. The fragments of Nc-p43 were as follow: a total open reading frame (OFR), T: OFR without signal sequence and C-terminal hydrophobic sequence, S: N-terminal 2/3 parts of S, A: C-terminal 2/3 parts, P; N-terminal 1/3 part, X: middle 1/3 part Y; and C-terminal 1/3 part, Z, respectively. The DNA fragments were cloned into pGEX-47 vector. Recombinant plasmids transformed into Escherichia coli of BL21 pLysS (DE3) strain were induced to express GST or GST fused fragments of Nc-p43 such as 69 kDa protein for T,66 kDa for S, 52 kDa for A,53 kDa for P, and 40 kDa proteins for X, Y, and Z, respectively in SDS-PAGE. The Nc-p43 fragments of T, S, and P reacted with a bovine serum of neosporosis while those of A, X, Y, and Z together with GST did not in the western blot. These findings suggest that the antigenic domain of Nc-p43 of N. caninum may be localized in the C-terminal 2/3 parts. Together with Al9 clone in SAGI of Toxoplasma gondii (Nam et at., 1996), the P fragment of Nc-p43 could be used as efficient antigens to diagnose and differentiate those infections with both species .

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2495-2499
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• 2015

Mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 3 (MEKK3) is an important serine/threonine protein kinase and a member of the MAPK family. MEKK3 can effectively activate the MEK/ERK signaling pathway and promote an autocrine growth loop critical for tumor genesis, cell proliferation, terminal differentiation, apoptosis and survival. To explore the relationship between MEKK3 and cell apoptosis, clinicopathology and prognosis, we characterize the expression of MEKK3, pERK and FoxP3 in the renal clear cell carcinoma (RCCC). Protein expression was detected by tissue microarray and immunochemistry in 46 cases of RCCC and 28 control cases. Expression levels of CD3+,CD3+CD4+,CD3+CD8+,CD4+CD25+, CD4+CD25+ FoxP3+ were assessed by flow cytometry and analyzed for their association with pathological factors, correlation and prognosis in RCCC. Expression of MEKK3, pERK and FoxP3 was significantly up-regulated in RCCC as compared to control levels (p<0.01), associated with pathological grade (p<0.05)and clinical stage (p<0.05). CD4+CD25+ Foxp3+ Treg cells were also significantly increased in RCCC patients (p<0.05). Cox multivariate regression analysis showed that MEKK3, pERK expression and patholigical stage were independent prognostic factors in patients with RCCC (p<0.05). MEKK3 can be used as an important marker of early diagnosis and prognostic evaluation in RCCC. It may be associated with imbalance of anti-tumor immunity and overexpression of pERK. Expression of MEKK3 and pERK are significantly increased in RCCC, with protein expression and clinical stage acting as independent prognostic factors.

• Communications of the Korean Mathematical Society
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• v.15 no.1
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• pp.37-44
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• 2000

In this paper, for a given p-adic quasicharacter $c_{v}$ : $k_{v}$longrightarrow $Q_{p}$ satisfying a special condition, we will explicitly construct an admissible pairing corresponding to $c_{v}$. We define a p-adic height on the arbitrary abelian varieties associated to divisors and $c_{v}$ by using admissible pairings at every nonarchimedean places. We also show that our p-adic height satisfies similar properties of Neron-Tate's canonical p-adic height.t.ght.t.t.

• Journal of applied mathematics & informatics
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• v.36 no.5_6
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• pp.555-565
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• 2018

The main subject of this paper is to introduce the (p, q)-Euler polynomials and obtain several interesting symmetric properties of the modified (p, q)-Hurwitz Euler Zeta function with regard to (p, q) Euler polynomials. In order to get symmetric properties, we introduce the new (p, q)-analogue of Euler polynomials $E_{n,p,q}(x)$ and numbers $E_{n,p,q}$.

• Journal of Powder Materials
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• v.14 no.2 s.61
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• pp.108-115
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• 2007

Cu-Ni-P alloy nano powders were fabricated by the electrical explosion of electroless Ni plated Cu wires. The effect of applied voltage on the explosion was examined by applying pulse voltage of 6 and 28 kV, The estimated overheating factor, K, were 1.3 for 6 kV and 2.2 for 28 kV. The powders produced with pulse voltage of 6 kV were composed of Cu-rich solid solution, Ni-rich solid solution, and $Ni_3P$ phase. While, those produced with 28 kV were complete Cu-Ni-P solid solution and small amount of $Ni_3P$ phase. The initial P content of 6.5 at.% was reduced to 2-3 at.% during explosion due to its high vapour pressure.

• Biomolecules & Therapeutics
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• v.23 no.2
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• pp.189-194
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• 2015

P450 1A2 is responsible for the metabolism of clinically important drugs and the metabolic activation of environmental chemicals. Genetic variations of P450 1A2 can influence its ability to perform these functions, and thus, this study aimed to characterize the functional significance of three P450 1A2 allelic variants containing nonsynonymous single nucleotide polymorphisms (P450 $1A2^*8$, R456H; $^*15$, P42R; $^*16$, R377Q). Variants containing these SNPs were constructed and the recombinant enzymes were expressed and purified in Escherichia coli. Only the P42R variant displayed the typical CO-binding spectrum indicating a P450 holoenzyme with an expression level of ~ 170 nmol per liter culture, but no P450 spectra were observed for the two other variants. Western blot analysis revealed that the level of expression for the P42R variant was lower than that of the wild type, however the expression of variants R456H and R377Q was not detected. Enzyme kinetic analyses indicated that the P42R mutation in P450 1A2 resulted in significant changes in catalytic activities. The P42R variant displayed an increased catalytic turnover numbers ($k_{cat}$) in both of methoxyresorufin O-demethylation and phenacetin O-deethylation. In the case of phenacetin O-deethylation analysis, the overall catalytic efficiency ($k_{cat}/K_m$) increased up to 2.5 fold with a slight increase of its $K_m$ value. This study indicated that the substitution P42R in the N-terminal proline-rich region of P450 contributed to the improvement of catalytic activity albeit the reduction of P450 structural stability or the decrease of substrate affinity. Characterization of these polymorphisms should be carefully examined in terms of the metabolism of many clinical drugs and environmental chemicals.

• Journal of Microbiology and Biotechnology
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• v.15 no.4
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• pp.694-700
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• 2005

Defects in the mitotic spindle or in the attachment of chromosomes to the spindle are believed to release an activated form of spindle checkpoint complex that inhibits APC-dependent ubiquitination and subsequently arrests the cell cycle at metaphase. When the spindle assembly is disrupted, the fission yeast mitotic arrest deficient (mad) mutants fail to arrest and rapidly lose viability. To enhance our understanding of the molecular mechanisms for the pathway of checkpoint function, the functional characterizations of Mad 1 p from Schizosaccharomyces pombe involved in this process have been carried out. Yeast two-hybrid and various deletion analyses of S. pombe Mad1 p reveal that the C terminus of Mad1p is critical for the binding of Mad2p and maintenance of Mad 1 p-Mad2p interaction. In addition, it was found. that the Mad1p region (residues 206-356) is essential for Mad1p-other checkpoint components. Mad1p truncating this region is sufficient to bind Mad2p but abolishes the checkpoint function, indicating that the checkpoint function is necessary for interaction of Mad 1 p-other checkpoint components. The possible functions of S. pombe Mad1p at the cell cycle checkpoint are discussed.

• BMB Reports
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• v.32 no.3
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• pp.232-238
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• 1999

Sex differences in the induction of microsomal cytochrome P-450 (CYP) and the activities of several related enzymes of Sprague-Dawley rats treated with 1-bromopropane (1-BrP) were investigated. Male and female rats were exposed to 50, 300, and 1800 ppm of 1-BrP per kg body weight (6 h a day,S days a week, 8 weeks) by inhalation. The mean body weight of 1-BrP treated groups increased according to the day elapsed, but four and five weeks respectively after the start of the exposure, the mean body weight of male and female rats had significantly reduced in the group treated with 1800 ppm 1-BrP compared with the control group (p<0.01). While the relative weights of liver increased in both sexes, statistical significance in both sexes was found only in the group receiving 1800 ppm/kg of 1-BrP (p<0.01). The total contents of CYP, $b_5$, NADPH-P-450 reductase, NADH $b_5$ reductase, ethoxyresorufin-O-deethylase (EROD), pentoxyresorufin-O-dealkylase (PROD), and p-nitrophenol hydroxylase (pNPH) activities were examined for the possible effects of 1-BrP. No significant changes in the CYP and $b_5$ contents, NADPH-P-450 reuctase, NADH $b_5$ reductase, ethoxyresorufin-O-deethylase (EROD), and pentoxyresorufin- O-dealkylase (PROD) were observed between the control and treated groups. The activity of pNPH increased steadily with the increase in the concentration of 1-BrP in both sexes, but was significantly increased only in the 1800 ppm-treated group of male rats (p<0.05). When Western blottings were carried out with three monoclonal antibodies (MAb 1-7-1, MAb 2-66-3, and MAb 1-98-1) which were specific against CYP1A1/2, CYP2B1/2, and CYP2E1, respectively, a strong signal corresponding to CYP2E1 was observed in microsomes obtained from rats treated with 1-BrP. Glutathione S-transferase (GST) activity and the content of lipid peroxide significantly increased in the treated groups compared with the control group (p<0.05). These results suggest that 1-BrP can primarily induce CYP2E1 as the major form and that GST phase II enzymes play important roles in 1-BrP metabolism, showing sex-dependence in the metabolic mechanism of 1-BrP in the rat liver.

• Journal of the Korean Society of Physical Medicine
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• v.6 no.4
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• pp.425-436
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• 2011

Purpose : The purpose of this study is to identify static, dynamic balance performances and foot pressure after eye movement exercise in normal adults. Method : This study was performed on normal 18 males and 18 females subjects. They were divided into 3 groups using random sampling and executed saccadic eye movement, vestibuloocular eye movement, pursuit vergence eye movement exercise respectively. In order to compare the difference of balance and foot pressure, the subjects were measured before, middle and after eye movement. Balance was examined using the balance master 7.0 version systems. Foot pressure was examined using the gaitview AFA-50. Result : The result are as follows. 1. After first intervention, the difference of the FORM-EC item on MCTSIB variable in the vestibuloocular group was significantly decreased(p<.05) and after second intervention, the difference of the FIRM-EO(p<.05), FORM-EO(p<.01), FORM-EC(p<.01) items on MCTSIB variable in the saccadic group and FORM-EC(p<.05) item in the vestibuloocular group were significantly decreased respectively. 2. After first intervention, the difference of the EPE, MXE items in the saccadic group(p<.05) and RT(p<.05), EPE(p<.01) items in the pursuit vergence group were significantly decreased respectively after second intervention. 3. After first intervention, the difference of the Static A-P(p<.05), L-R(p<.01) and Dynamic A-P(p<.01), L-R (p<.01) items on foot pressure variable in the saccadic group, Dynamic A-P(p<.01) item in the vestibuloocular group and Static A-P(p<.01), Dynamic A-P(p<.05), L-R(p<.01) items in the pursuit vergence group were significantly decreased respectively. 4. After first intervention, the difference of the FORM-EO(p<.05), FORM-EC(p<.01) items and after second intervention, the difference of the FIRM-EO(p<.01) item on MCTSIB variable among three groups was significantly decreased respectively. Conclusion : Selective eye movement exercise program influences balance control ability and foot pressure of normal adults in positive ways.