• Title/Summary/Keyword: $T_{b}$

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Growth Inhibition of Escherichia coli during Heterologous Expression of Bacillus subtilis Glutamyl-tRNA Synthetase that Catalyzes the Formation of Mischarged Glutamyl-$tRNA_{l}$$^{Gln}$

  • Baick, Ji-Won;Yoon, Jang-Ho;Suk Namgoong;Dieter Soll;Kim, Sung-Il;Eom, Soo-Hyun;Hong, Kwang-Won
    • Journal of Microbiology
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    • v.42 no.2
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    • pp.111-116
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    • 2004
  • It is known that Bacillus subtilis glutamyl-tRNA synthetase (GluRS) mischarges E. coli $tRNA_{1}$$^{Gln}$ with glutamate in vitro. It has also been established that the expression of B. subtilis GluRS in Escherichia coli results in the death of the host cell. To ascertain whether E. coli growth inhibition caused by B. subtilis GluRS synthesis is a consequence of Glu-$tRNA_{1}$$^{Gln}$ formation, we constructed an in vivo test system, in which B. subtilis GluRS gene expression is controlled by IPTG. Such a system permits the investigation of factors affecting E. coli growth. Expression of E. coli glutaminyl-tRNA synthetase (GlnRS) also amelio-rated growth inhibition, presumably by competitively preventing $tRNA_{1}$$^{Gln}$ misacylation. However, when amounts of up to 10 mM L-glutamine, the cognate amino acid for acylation of $tRNA_{1}$$^{Gln}$, were added to the growth medium, cell growth was unaffected. Overexpression of the B. subtilis gatCAB gene encoding Glu-$tRNA^{Gln}$ amidotransferase (Glu-AdT) rescued cells from toxic effects caused by the formation of the mis-charging GluRS. This result indicates that B. subtilis Glu-AdT recognizes the mischarged E. coli Glu-$tRNA_{1}$$^{Gln}$, and converts it to the cognate Gln-$tRNA_{1}$$^{Gln}$ species. B. subtilis GluRS-dependent Glu-$tRNA_{1}$$^{Gln}$ formation may cause growth inhibition in the transformed E. coli strain, possibly due to abnormal protein synthesis.

An empirical study on RFM-T model for market performance of B2B-based Technology Industry Companies (B2B 중심의 기술 산업 기업의 수익성 성과를 위한 RFM-T 모형 실증 연구)

  • Miyoung Woo;Young-Jun Kim
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.24 no.2
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    • pp.167-175
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    • 2024
  • Due to the Fourth Industrial Revolution, ICT(Information and Communication Technology) industry is becoming more important and sophisticated than ever. In B2B based ICT industry demand forecasting by analyzing the previous customer data is so important. RFM, one of customer relationship management models is a marketing technique that evaluates Recency, Frequency and Monetary value to predict customers behavior. RFM model has been studied focusing on the B2C based industry. On the other hand there is a lack of research on B2B based technology industry. Therefore this study applied it to B2B based high technology industry and considered T(technology collaboration) value, which are identified as important factors in the technology industry. To present an improved model for market performance in B2B technology industry, an empirical study was conducted on comparing the accuracy of the traditional RFM model and the improved RFM-T model. The objective of this study is to contribute to market performance by presenting an improved model in B2B based high technology industry.

A CAMERON-STORVICK THEOREM ON C2a,b[0, T ] WITH APPLICATIONS

  • Choi, Jae Gil;Skoug, David
    • Communications of the Korean Mathematical Society
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    • v.36 no.4
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    • pp.685-704
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    • 2021
  • The purpose of this paper is to establish a very general Cameron-Storvick theorem involving the generalized analytic Feynman integral of functionals on the product function space C2a,b[0, T]. The function space Ca,b[0, T] can be induced by the generalized Brownian motion process associated with continuous functions a and b. To do this we first introduce the class ${\mathcal{F}}^{a,b}_{A_1,A_2}$ of functionals on C2a,b[0, T] which is a generalization of the Kallianpur and Bromley Fresnel class ${\mathcal{F}}_{A_1,A_2}$. We then proceed to establish a Cameron-Storvick theorem on the product function space C2a,b[0, T]. Finally we use our Cameron-Storvick theorem to obtain several meaningful results and examples.

ON KATO`S DECOMPOSITION THEOREM

  • YONG BIN CHOI;YOUNG MIN HAN;IN SUNG HWANG
    • Communications of the Korean Mathematical Society
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    • v.9 no.2
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    • pp.317-325
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    • 1994
  • Suppose X is a complex Banach space and write B(X) for the Banach algebra of bounded linear operators on X, X* for the dual space of X, and T*$\in$ B(X*) for the dual operator of T. For T $\in$ B(X) write a(T) = dim T$^{-1}$ (0) and $\beta$(T) = codim T(X).(omitted)

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Effects of Combined Micronutrient(Fe, Mn, Cu, Zn, Mo and B) Application on Forage Traits in Pure and Mixed Swards of Orchardgrass and White Clover II. Changes in the yields and concurrence index of forage plants (Orchardgrass 및 White Clover의 단파 및 혼파 재배에서 미량요소(Fe, Mn, Cu, Zn, Mo, B)의 조합시비가 목초의 여러 특성에 미치는 영향 II. 초종별 건물수량 및 식생 경합지수의 변화)

  • Jung, Yeun-Kyu
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.23 no.4
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    • pp.237-246
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    • 2003
  • This pot experiment was conducted in order to find out the effects of application of combined micronutrients(T$_1$: control. T$_2$; Fe, T$_3$; Fe+Mn, T$_4$; Fe+Mn+Cu, T$_{5}$; Fe+Mn+Cu+Zn, T$_{6}$;Fe+Mn+Cu+Zn+Mo, T$_{7}$; Fe+Mn+Cu+Zn+Mo+B) on forage performance of pure and mixed cultures of orchardgrass and white clover The 2nd part was concerned with the changes in the forage yields and concurrence index. The results obtained are summarized as follows: 1. The effects of combined micronutrient applications on the forage yields were different according to the forage species, whether it was a pure or mixed cultures, and additional fertilization(especially N). The effects of them on the forage productivity and botanical composition were more obvious in white clover, especially in mixed culture, than in orchardgrass. By the significant role of B as a regulator, the yields of both forages were best in the T$_{7}$, respectively. 2. In the pure culture, the high yields of both forages were obtained by the T$_{7}$ and T$_2$, whereas the T$_{6}$ and T$_3$resulted in the low yields. The best yields of both forages were obtained by the T$_{7}$ with relatively optimum ratios among the micronutrients as follows; Fe/Mn/Cu/Zn, Fe/Mo, Mo/B, and ∑ cation/∑anion. It was observed the multiple interaction of Fe${\times}$Mn${\times}$Mo${\times}$B, and the significant role of B as a regulator. The effects of them on white clover were more distinct at no additional fertilization than at the additional fertilization(especially N). 3. In mixed culture, the optimum applications of them resulted in the positive increase of yield and botanical composition of white clover, whereas orchardgrass tended to be inversely except the T$_{7}$.X> 7/. 7/.

Effects of planting density on the production of pepper for mechanized production operation

  • Kwak, Su-Ji;Han, Jae-Woong;Kwak, Eun-Ji;Kim, Woong
    • Korean Journal of Agricultural Science
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    • v.45 no.4
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    • pp.839-845
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    • 2018
  • As a solution to the rural shortage of labor, mechanization crop production is necessary, but in some cases, the mechanization can cause problems such as a decrease in products due to the expansion of the necessary moderate workspace. The purpose of this study was to compare the yields of pepper by the planting-density for the mechanization of pepper cultivation. Experiments were done with three planting-density levels of $900{\times}300mm^2$(A-T), $1200{\times}450mm^2$(B-T), and $1500{\times}600mm^2$(C-T). In the analysis of growth, the highest values in plant height and thickness and the number of branches were observed with the B-T. C-T showed the highest values in the number of green-pepper and red-pepper and weight of the green-pepper and red-pepper, followed by B-T and A-T. In the analysis of growth, it was concluded that the proportion of the pepper body to the total length increased as the planting-density decreased. C-T had the biggest maximum diameter of the body, followed by B-T and A-T. On the other hand, A-T had the biggest minimum diameter of the body, followed by B-T and C-T. It was judged that the larger the planting-density was, the shorter the length was and the thicker the form was. As a result of measuring the chromaticity, there was no significant statistical difference in quality. Based on the experiment results, the ranking in total yields was in the order of C-T, B-T, and A-T. The reduced planting-density seemed to increase the productivity, while the labor intensity and time were reduced due to the improvement of the working environment.

Kinetics of IFN-${\gamma}$ and IL-17 Production by CD4 and CD8 T Cells during Acute Graft-versus-Host Disease

  • Ju, Ji-Min;Lee, Hakmo;Oh, Keunhee;Lee, Dong-Sup;Choi, Eun Young
    • IMMUNE NETWORK
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    • v.14 no.2
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    • pp.89-99
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    • 2014
  • Graft-versus-host disease (GVHD) is a fatal complication that occurs after allogeneic hematopoietic stem cell transplantation. To understand the dynamics of CD4 and CD8 T cell production of IFN-${\gamma}$ and IL-17 during GVHD progression, we established a GVHD model by transplanting T cell-depleted bone marrow (TCD-BM) and purified T cells from B6 mice into irradiated BALB.B, creating an MHC-matched but minor histocompatibility (H) antigen-mismatched transplantation (B6 ${\rightarrow}$ BALB.B GVHD). Transplantation-induced GVHD was confirmed by the presence of the appropriate compositional changes in the T cell compartments and innate immune cells in the blood and the systemic secretion of inflammatory cytokines. Using this B6 ${\rightarrow}$ BALB.B GVHD model, we showed that the production of IFN-${\gamma}$ and IL-17 by CD4 T cells preceded that by CD8 T cells in the spleen, mesenteric lymph node, liver, and lung in the BALB.B GVHD host, and Th1 differentiation predated Th17 differentiation in all organs during GVHD progression. Such changes in cytokine production were based on changes in cytokine gene expression by the T cells at different time points during GVHD development. These results demonstrate that both IFN-${\gamma}$ and IL-17 are produced by CD4 and CD8 T cells but with different kinetics during GVHD progression.

Studies on the application of bone alkaline phosphatase and percentage of BALP values to total alkaline phosphatase as a marker of bone formation (골혈성 지표로써 bone alkaline phosphatase(BALP)와 TALP치에 대한 BALP치 비율(B/T)의 응용)

  • Kim, Nam-soo;Choi, In-hyuk
    • Korean Journal of Veterinary Research
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    • v.39 no.6
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    • pp.1197-1209
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    • 1999
  • Twenty-one dogs(male 11 heads, female 10 heads) which were about 16 months ($16.3{\pm}3.5$) old and 10kg($10.1{\pm}2.0$) body weight, were allotted randomly into four groups as follows. Group I consisted of five dogs whose muscles were operated for sham muscle injuries. Group II consisted of seven dogs treated for cystic duct obstruction. Group III consisted of five dogs treated for the union fracture model. Group IV consisted of five dogs treated for the non-union fracture model. Radiographical and histological observations were carried out to determine bone alkaline phosphatase(BALP) and total alkaline phosphatase(TALP) values of each group for 20 weeks after the treatments with the condition of new bone formation. And also the applicability of percentage of BALP values to TALP (B/T) was studied after BALP was compared respectively with TALP. The level of TALP was increased without any relation to bone formation in group II, and all levels of BALP and B/T were increased in group III. The mean of B/T was high in statistical significance, due to varied levels of B/T and BALP. The changes of rates of B/T were significantly increased only in the case of the active new bone formation in group III, union fracture model. It was recognized that the mean values of B/T were statistical significant of the high applicability of the B/T ratio as an index of bone formation.

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Tc and Jc distribution in in situ processed MgB2 bulk superconductors with/without C doping

  • Kim, C.J.;Kim, Y.J.;Lim, C.Y.;Jun, B.H.;Park, S.D.;Choo, K.N.
    • Progress in Superconductivity and Cryogenics
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    • v.16 no.2
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    • pp.36-41
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    • 2014
  • Temperature dependence of magnetic moment (m-T) and the magnetization (M-H) at 5 K and 20 K of the in situ processed $MgB_2$ bulk pellets with/without carbon (C) doping were examined. The superconducting critical temperature ($T_c$), the superconducting transition width (${\delta}T$) and the critical current density ($J_c$) were estimated for ten test samples taken from the $MgB_2$ bulk pellets. The reliable m-T characteristics associated with the uniform $MgB_2$ formation were obtained for both $MgB_2$ pellets. The $T_cs$ and ${\delta}Ts$ of all test samples of the undoped $MgB_2$ were the same each other as 37.5 K and 1.5 K, respectively. The $T_cs$ and ${\delta}Ts$ of the C-doped $MgB_2$ were 36.5 K and 2.5 K, respectively. Unlike the m-T characteristics, there existed the difference among the M-H curves of the test samples, which might be caused by the microstructure variation. In spite of the slight $T_c$ decrease, the C doping was effective in enhancing the $J_c$ at 5 K.

Comparison of temperature Derived from the Microwave Sounding Unit and Radiosonde Observation Data in Korea (한반도 지역의 마이크로파 위성자료와 고층관측자료의 비교)

  • 김소현;황병준;안명환;정효상;김금란
    • Korean Journal of Remote Sensing
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    • v.16 no.1
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    • pp.47-54
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    • 2000
  • We compared the satellite observed temperature with the radiosonde observed temperature in the Korean Peninsula. The radiosonde observed data were obtained from four upper air observation stations in the Korean Peninsula from 1981 to 1998, and were compared with the satellite observed data of the channel-2 and channel-4 of microwave sounding unit(MSU) on board NOAA series of polar-orbiting satellites. The radiosonde data were reconstructed from radiosonde T$_b$ using MSU weighting function. The monthly climatology shows radiosonde T$_{b2}$ is higher than MSU T$_{b2}$ in summer. The correlation between MSU T$_{b2}$ and radiosonde T$_{b2}$ is 0.72-0.76 and 0.73-0.81 between MSU T$_{b4}$ and radiosonde T$_{b4}$. The T$_{b2}$ show a positive trend and the T$_{b4}$ has a negative trend during the 18 years.