• Journal of the Korean earth science society
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• v.35 no.2
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• pp.104-114
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• 2014

The variation of acidity, conductivity, and ion components in precipitation depending on the dominant wind direction was investigated from January, 2009 to December 2009 in Busan, Korea. Both southwesterly and northeasterly winds were dominant in Busan area. The volume-weighted mean acidity showed pH 7, and the high conductivity indicated 200 ${\mu}scm^{-1}$ in westerly wind. The volume-weighted mean equivalent concentration showed higher value of $K^+$ and $Cl^-$ in all wind directions. The composition ratio of $NO{_3}^-/SO{_4}^{2-}$ showed over 3 in northerly wind. The neutralization factors have been found to have higher value for potassium ion in northeasterly, easterly, southwesterly, and westerly winds compared with different wind directions, which indicated significant neutralization of acidic components over the region by potassium. Also, the concentration of sea salt has been found over 800 ${\mu}sm^{-3}$ in northeasterly and southwesterly winds. Air masses passing through Manchuria, Inner Mongolia plateau, China, and Russia in spring, autumn, and winter covered Busan, Korea in northerly, westerly, and northwesterly winds. However, air masses passing through the ocean in summer covered Busan, Korea in easterly, northeasterly, and southwesterly winds. Therefore, the variation of acidity, conductivity, and ionic components contained in precipitation shows each seasonal characteristics with prevailing wind systems between the continental and coastal area in Busan, Korea.

• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.235-242
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• 2004

A system for the production of transgenic plants has been developed for Italian ryegrass(Lolium mult리orum Lam.) via Agrobacterium-mediated transformation of embryogenic callus. Mature seed-derived calli were infected and co-cultured with Agrobacterium EHA101 carrying standard binary vector pIG121Hm encoding the hygromycin phosphotransferase(HPT), neomycin phosphotransferase II (NPTII) and intron-oontaining $\beta$g1ucuronidase( intron-GUS) genes in the T-DNA region. The effects of several factors on transformation and the expression of the GUS gene were investigated. Inclusion of 200${\mu}M$ acetosyringone(AS) in inoculation and co-cultivation media lead to a significant increase in stable transformation efficiency. Increasing Agrobacterium cell density up to 1.0 in $OD_{600}$ during infection increased transfonnation efficiency of embryogenic calli. The highest transfonnation efficiency was obtained when embryogenic calli were incoulated with Agrobacterium in the presence of 0.1% Tween20 and 200${\mu}M$ AS. Hygromycin resistant calli were developed into complete plants via somatic embryogenesis. GUS histochemical assay and PCR analysis of transgenic plants demonstrated that transgenes were integrated into the genome of Italian ryegrass.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.8
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• pp.324-331
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• 2017

This study consisted of methodological research verifying the validity and reliability of the Korean version of the interpersonal emotion management (IEM) strategies scale after translating and modifying the scale developed by Little et al. The study verified the content validity, construct validity, concurrent validity and reliability based on 189 nurses working at four hospitals in the Seoul and Gyeonggi region. In this study, the Korean version of the IEM strategies scale was comprised of four factors, with a total of 15 questions that included three regarding situation modification, three regarding attention deployment, four regarding cognitive change, and five on modifying emotional response. Furthermore, verification of concurrent validity revealed that the Korean version of the IEM strategies scale was a valid tool as the correlation of the emotional intelligence scale appeared as r=0.60 (p<0.001). The reliability verification showed four factors at Cronbach's ${\alpha}=0.84-90$, confirming that the Korean version of the tool in this study was very reliable.It is important to note that this study provided basic data for the development of educational programs that can effectively control the negative emotions of clinical nurses at the organization level.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.2
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• pp.122-128
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• 2013

This study was carried out to evaluate the soil dehydrogenase activity and microbial biomass C with soil type and land use in cropland of JeJu region. Soil chemical properties, dehydrogenase activity, and microbial biomass C were analyzed after sampling from upland (50 sites), orchard (50 sites), paddy (30 sites), horticultural facility (30 sites) in March. Average pH values was at 6.3 in upland soil, however soil chemical properties showed a large spatial variations in both orchard and horticultural facility soil. The Zn and Cu contents increased by the continuous application of pig manure compost in some citrus orchard soil. Soil dehydrogenase activity and microbial biomass C were higher in non-volcanic ash than in volcanic ash soil regardless of land use type. Soil dehydrogenase activity was two to four times higher in upland than in the others. It was at 38.7 ug TPF $24^{h-1}g^{-1}$ in non-volcanic ash of upland soil. Microbial biomass C content was very high in horticultural facility soil and it showed at 216.8 $mg\;kg^{-1}$ in non-volcanic ash. Soil dehydrogenase activity showed a positive correlation with organic matter ($r^2$=0.59), Zn ($r^2$=0.65), and Cu ($r^2$=0.66) in non-volcanic ash horticultural facility soil. There was a negative correlation ($r^2$=0.57) between soil organic matter and dehydrogenase activity in volcanic ash upland soil.

• The Korean Journal of Mycology
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• v.26 no.3 s.86
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• pp.354-360
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• 1998

We isolated three amplified DNA fragments from P. sajor-caju by Polmerase chain reaction (PCR) using the chithin synthase specific primers. Since the sequence analysis of the these fragments showed significant homology to the other known chitin synthase gene, we regarded these cloned fragments as PsCHS1, PsCHS2, and PsCHS3 according to their size. The PsCHS3, which showed the highest sequence homology (83% identity in amino acid level with ChsI of Rhizopus oligosporus in conserved region), was selected to see expression pattern of the corresponding gene. The result of RT-PCR using internal primer of the PsCHS3 fragment revealed that PsCHS3 gene was only expressed in cap and mycelium but not in stipe. In order to see whether the PsCHS3 gene was to be induced by wounding, the comparison of the mRNA level of this gene between wounded and unwounded mature cap showed at least two times induction of this gene by wounding treatment.

• Journal of Life Science
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• v.13 no.4
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• pp.529-534
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• 2003

This study was done to investigated the phytosterol compositions of safflower (Carthamus tinctorius L.) seed. The phytoestrogen activity was also determined using CAT-ELISA Kit in ethanol extract of safflower seed. The phytosterol of safflower seeds was identified using gas chromatography-mass spectrometry after saponification of the oils. The phytosterol content and composition of safflower seed oils were 4% and identified stigmast-5-en-3-ol (3$\beta$, 24S)-form, ${\gamma}$-sitosterol (clionasterol) with Wiley MS spectrum library. The synergistic effect of human estrogen receptor (hER) has been investigated using a minimal chimeric promoters composed of the TATA region of the adenovirus-2 major late promoter (A22MLP) and two consensus perfectly polindromic Xenopus vitellogenin A2 gene estrogen responsive elements (XVEREl19). Transient transfection experiments in tile human breast adenocarcinoma cell line MCF-7, which is known to express the estrogen receptor endogenously, revealed that phytoestrogen from Carthamus tinctorius L. acts as estrogen. We have observed the transcriptional activities stimulated methanol and ethanol extract of safflower seed in MCF-7, were 0.43 and 0.37 respectively, compared to that by $\beta$-estradiol as 1.0. Our data showed that safflower seeds have estrogenic activity methanol and ethanol extracts and ethanol lower than that of $\beta$-estradiol. This result provides the first evidence that the beneficial effect of safflower seeds may be mediated, at least in part, by the stimulating effect of phytoestrogen ell bone-protecting.

• Molecules and Cells
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• v.40 no.5
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• pp.346-354
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• 2017

Long interspersed nuclear element-1 (LINE-1) is a retrotransposon that contains a CpG island in its 5'-untranslated region. The CpG island of LINE-1 is often heavily methylated in normal somatic cells, which is associated with poor prognosis in various cancers. DNA methylation can differ between formalin-fixed paraffin-embedded (FFPE) and frozen tissues. Therefore, this study aimed to compare the LINE-1 methylation status between the two tissue-storage conditions in gastric cancer (GC) clinical samples and to evaluate whether LINE-1 can be used as an independent prognostic marker for each tissue-storage type. We analyzed four CpG sites of LINE-1 and examined the methylation levels at these sites in 25 FFPE and 41 frozen GC tissues by quantitative bisulfite pyrosequencing. The LINE-1 methylation status was significantly different between the FFPE and frozen GC tissues (p < 0.001). We further analyzed the clinicopathological features in the two groups separately. In the frozen GC tissues, LINE-1 was significantly hypomethylated in GC tissues compared to their corresponding normal gastric mucosa tissues (p < 0.001), and its methylation status was associated with gender, differentiation state, and lymphatic and venous invasion of GC. In the FFPE GC tissues, the methylation levels of LINE-1 differed according to tumor location and venous invasion of GC. In conclusion, LINE-1 can be used as a useful methylation marker for venous invasion in both FFPE and frozen tumor tissues of GC.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.617-625
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• 2013

Stauntonia hexaphylla and Raphiolepis indica, cold-tolerant broadleaved evergreens ranging through the southern region of South Korea, were assessed on salt tolerance with NaCl treatment using visual damage, chlorophyll florescence image, and malondialdehyde (MDA) analysis. As NaCl concentrations increased, the soil pH decreased and EC increased, and the soil of S. hexaphylla was affected more strongly by the treatment than that of R. indica. In visual damage, S. hexaphylla withered above 200 mM NaCl at 20 days after the treatment. All individuals of R. indica survived during the experiment though the leaves of R. indica showed visual damages up to 400 mM NaCl. The color changes in chlorophyll fluorescence showed a strong correlation with the degree of visual damage. As NaCl increased, the red color of the leaves of S. hexaphylla was distinctly changed to blue and chlorophyll fluorescence decreased starting from the margin to the middle of a leaf. R. indica showed subtle color changes and remained in red color during the experiment. At five days after the NaCl treatment, the MDA of S. hexaphylla was above $4.56nmol{\cdot}g^{-1}$ when plants showed the highest visual damage and EC. The MDA of R. indica in all treatments showed below $1.5nmol{\cdot}g^{-1}$ except 400 mM NaCl treatment during the experiment.

• Korean Journal of Food Science and Technology
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• v.46 no.3
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• pp.309-314
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• 2014

To improve the standardized quality of gochujang produced by major companies, the quality characteristics of 29 gochujang samples produced by regional small- and medium-scale manufacturers were determined. The average moisture content, crude protein content, pH, acidity, and salinity of gochujang from regional manufacturers were found to be 34.32-45.53%, 5.07-5.68%, 4.61-4.92, 3.30-3.72%, and 9.93-16.14%, respectively. The contents of reducing sugar, amino type N, and capsaicinoids were found to be 16.81-29.81%, 191.72-660.55 mg%, and 32.49-79.58 mg/kg, respectively, and the ${\alpha}$- and ${\beta}$-amylase activity were 2.52-4.15 and 26.36-36.47 U/g, respectively. These values varied significantly across regional manufacturers. In addition, overall, gochujang had a range of 23.93-55.64% moisture content, 4.01-7.30% crude protein content, 105.47-1092.00 mg% amino type N content, and 8.74-181.50 mg/kg capsaicinoid content. These results indicate that the quality characteristics of gochujang showed a wide distribution but met the Korean Industrial Standards. Therefore, these data can be used for quality control of gochujang.

• Resources Recycling
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• v.29 no.5
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• pp.28-37
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• 2020

In this study, the effect of precipitation temperature, ammonium chloride amount and addition method, vanadium and sodium hydroxide content of the solution on the precipitation of ammonium metavanadate were examined by using the sodium vanadate(NaVO₃) solution in alkali region as a starting material. As the pH of solution decreased, the addition amount of ammonium chloride and the vanadium content of the solution increased, the precipitation rate of ammonium metavanadate increased. In this research condition, the basic conditions for obtaining more than 90% of precipitation yield were 10,000mg/L of vanadium content, 2equivalents of ammonium chloride addition, room temperature, and 2 hours of precipitation time. The size of precipitated particles decreased with increasing precipitation rate. Especially when liquid ammonium chloride was injected into the solution, the precipitation rate was the slowest and the particle size of the precipitate was the largest. After the primary precipitation by adding ammonium chloride as a solid, the secondary precipitation was carried out by adding new reactants. At this time, the precipitation with added ammonium chloride solid was not affected by the precipitates present in the solution. However, when liquid ammonium chloride was added, new precipitate was deposited on the surface of the precipitate present in the solution, increasing its size. Due to the difference in ammonium metavanadate solubility to temperature, the precipitation temperature at the vanadium content of 10,000mg/L in the solution affected the precipitation rate of ammonium metavanadate and the precipitation temperature did not affect the precipitation rate at a high concentration of more than 30,000mg/L vanadium content in the solution.