• Title/Summary/Keyword: $DPPH\

Search Result 6,006, Processing Time 0.033 seconds

Phytotoxic Effect, DPPH Radical Scavenging Activity and Chlorogenic Acid Level of Methanol Extracts from Aerial Parts of Several Korean Salad Plants

  • Chon Sang-Uk;Kim Young-Min;Kim Dong-Kwan;Heo Buk-Gu;Cho Ja-Yong
    • Korean Journal of Plant Resources
    • /
    • v.19 no.3
    • /
    • pp.405-410
    • /
    • 2006
  • A series of aqueous or methanol extracts from four different Korean salad plants were assayed to determine their allelopathy and antioxidant activity. The extracts applied on filter paper in a Petri-dish bioassay significantly inhibited root growth of against alfalfa (Medicago sativa) seedlings. Leaf extracts from 40 g dry tissue $L^{-1}$ of Aster yomena was most phytotoxic to alfalfa root growth, and followed by that of Cirsium japonicum, Taraxacum officinale, and Ixeris dentate. Methanol extracts of plants dose-dependently increased DPPH free radical scavenging activity in vitro. Antioxidant activity of methanol extracts from the same plant species was investigated, and the result showed high DPPH free radical scavenging activity in Cirsium japonicum, Aster yomena, and Ixeris dentate, however, in Taraxacum officinale was least activity. By means of HPLC analysis, chlorogenic acid, p-coumaric acid, and total phenolics with 7.68, 17.47 and 18.64 mg, $100g^{-1}$, respectively, showed the highest amounts in methanol extracts from Cirsium japonicum leaves. These results suggest that Compositae salad plants contain water-soluble substances with allelopathic potential as well as antioxidant activity.

Equivalence of Mixed and Individual Preparation of Galgeun-tang in terms of Polyphenol Contents and DPPH Radical Scavenging Activity (폴리페놀 및 DPPH 라디칼 소거활성 관점에서의 복합갈근탕 및 단미혼합갈근탕의 동등성)

  • Kim, Dong-Seon;Um, Young-Ran;Yang, Min-Chul;Yun, Na-Young;Jung, Sang-Won;Lee, Jae-Hoon;Ma, Jin-Yeul
    • YAKHAK HOEJI
    • /
    • v.55 no.1
    • /
    • pp.45-48
    • /
    • 2011
  • Traditional medicines usually use combinational formula that are prepared by mixing different varieties of medicinal herbs and boiling them in water to yield a decoction. In recent years a modified method has been proposed and practiced wherein the individual herbs are boiled with water separately and later these extracts are mixed together for use. We attempted to evaluate their equivalence in terms of its polyphenol contents and DPPH radical scavenging activity. The polyphenol contents as well as the DPPH radical scavenging activities were very similar to each other.

Antioxidant Effect of Assai Palm Methanolic Extract (Assai 열매 메탄올 추출물의 항산화 효과)

  • Choi, Chul-Yung;Degrandi, Isolde Hoerbe;Cho, Sung-Hwan
    • Food Science and Preservation
    • /
    • v.18 no.6
    • /
    • pp.967-972
    • /
    • 2011
  • The methanolic product of assai(Euterpe oleracea Mart) scavenged the intracellular reactive oxygen species (ROS) and 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical, and prevented lipid peroxidation. The radical-scavenging activity of assai palm methanolic extract protected the viability of peritoneal macrophage cells exposed to hydrogen peroxide($H_2O_2$). Furthermore, the extract reduced the apoptotic-cell formation induced by $H_2O_2$, as demonstrated by the decrease in the number of hypodiploid cells and in the apoptotic-cell body formation. These results indicate that assai palm methanolic extract has radical-scavenging activity and ameliorates $H_2O_2$-induced cytotoxicity.

Optimization of Hot Water Extraction for Enhancing Antioxidative Activity from Auricularia auricula Using Taguchi Approach (Taguchi 법에 의한 흑목이버섯의 항산화활성 증진을 위한 열수추출 최적화)

  • Kim, Hyeon-Min;Hur, Won;Lee, Shin-Young
    • Food Engineering Progress
    • /
    • v.15 no.3
    • /
    • pp.221-229
    • /
    • 2011
  • Optimization study of the hot water extraction for enhancing antioxidative activity from Auricularia auricula was performed by Taguchi approach using orthogonal matrix $L_9(3^4)$ method. The correlation between DPPH radical scavenging activity and the components of samples extracted from different extraction conditions were also analyzed. The correlation coefficient between DPPH radical scavenging activity and melanin content of A. auricula were 0.93, indicating 'good correlation'. The optimum extraction conditions was obtained at the extraction time of 1 hr. temperature of $85^{\circ}C$, solid: water ratio of 1: 40(w/v) and frequency of 2 times. Under these conditions, values of maximum DPPH free radical scavenging activity and melanin contents of A. auricula were $67.21{\pm}2.17$ and $52.94{\pm}2.10 mg/g$, respectively. Melanin content of 1.6 times and DPPH free radical scavenging effect of 130% were enhanced by optimization.

Physiological characteristics of Extracts from Monascus-Fermented Chinese Yam by Monascus sp. strain (Monascus 속 균주를 이용한 발효 홍국마 추출물의 생리학적 특성)

  • Jeon, Chun-pyo;Park, Se-cheol;Lee, Joon-geol
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • 2014.05a
    • /
    • pp.829-832
    • /
    • 2014
  • This study was conducted to investigate antioxidative and physiological activities of ethanol extracts concentration from Monascus-Fermented Chinese Yam (MFCY). The ethanol extracts from MFCY were measured to examine pigments, DPPH radical scavenging activity, reducing power and monacolin K contents. As a results show that Monascus sp. MK805, with Dioscorea japonica as the substrate can produce pigments (yellow, orange and red), DPPH radical scavenging activity, reducing power and monacolin K content at 29.6 (yellow), 15.1 (orange), 20.4 (red), 72.8%, 1.74(OD at 700nm), 480.6 mg/kg in EtOH extract, respectively. Therefore, fermented chinese yam (Dioscorea japonica) was estimated to be effective biological activity material.

  • PDF

Quantitation and Radical Scavenging Activity Evaluation of Iridoids and Phenylethanoids from the Roots of Phlomis umbrosa (Turcz.) using DPPH Free Radical and DPPH-HPLC Methods, and their Cytotoxicity

  • Le, Duc Dat;Nguyen, Duc Hung;Zhao, Bing Tian;Min, Byung Sun;Song, Si Whan;Woo, Mi Hee
    • Natural Product Sciences
    • /
    • v.25 no.2
    • /
    • pp.122-129
    • /
    • 2019
  • The roots of Phlomis umbrosa (Turcz.) (Phlomidis Radix) have been traditionally used to treat cold, reduce swelling and staunch bleeding. Four iridoids (1 - 3 and 5) and six phenylethanoid derivatives (4, and 6 - 10) were isolated from the roots of P. umbrosa. A simple, sensitive, and reliable analytical HPLC/PDA method was developed, validated, and applied to determine 10 marker compounds in Phlomidis Radix. Furthermore, the isolates were evaluated for cytotoxic and anti-oxidant activities as well as DPPH-HPLC method. Among them, compounds 4 and 6 - 9 displayed potent anti-oxidant capacities using DPPH assay with $IC_{50}$ values of $27.7{\pm}2.4$, $10.2{\pm}1.1$, $18.0{\pm}0.8$, $19.1{\pm}0.3$, and $19.9{\pm}0.6{\mu}M$, and compounds 6, 8, and 9 displayed significant cytotoxic activity against HL-60 with $IC_{50}$ values of $35.4{\pm}3.1$, $18.6{\pm}2.0$, and $42.9{\pm}3.0{\mu}M$, respectively.

Antioxidant Activity Evaluation of Vegetable Complex Extract of Pre-Heat Treatment Process (사전 열처리 공정 처리한 야채류 복합추출물의 항산화활성 평가)

  • Kim, Hyun-Kyoung;Hwang, Hye-young;Kim, Heung-Tae;Kim, Hye-Jin;Seo, Soo-Jin
    • The Journal of the Convergence on Culture Technology
    • /
    • v.8 no.2
    • /
    • pp.409-416
    • /
    • 2022
  • As a food ingredient, the extract has been proven of its antioxidant properties and safe to be consumed. It's increased antioxidant activity was evaluated by comparing its activity to the complex extract of fruits and vegetables, which is known to exhibit both antioxidant and physiological activities. In this experiment, after pre-heat treatment process, the increased antioxidant extract showed 41% of DPPH radical scavenging activity. The remarkable antioxidant activity of the produced extract will become useful in creating cosmetic or health products.

Comparison of in vitro antioxidant capacities of Phragmites communis Trin. and Phragmites japonica Steud.

  • Kyeong Won Yun;Kyoung Sun Seo
    • Food Science and Preservation
    • /
    • v.30 no.6
    • /
    • pp.960-968
    • /
    • 2023
  • The rhizome of Phragmites communis Trin. is used for vomiting and belching by clearing stomach and the sprout is used as tea. Phragmites japonica is similar with P. communis except the color of sheath is purple. This study is aimed to compare the in vitro antioxidant activity, total polyphenol and flavonoids contents of P. communis and P. japonica. The antioxidant activities of fractions from the two Phragmites plants were evaluated by 1,1-diphenyl-2- picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay. The antioxidant activity varied with plant parts and extract solvents. The fractions of leaf extract from the two Phragmites plants (4.06±1.32-16.47±1.28%) showed higher antioxidant activity by DPPH assay compared with rhizome fractions of two Phragmites plants (0.00±0.00-14.15±0.07%), these are lower compared with ascorbic acid and butylhydroxyanisole (BHA). The highest ABTS radical scavenging activity was found for rhizome ether fraction, namely 74.95±0.56% and 73.04±1.85% for P. communis and P. japonica, these are higher than BHA. The total polyphenol and flavonoids contents were different with plant parts and extract solvents, likewise antioxidant activity. A significant correlation was shown between DPPH and ABTS radical scavenging activity. Considering the results of this study, the leaves and stems of P. communis and P. japonica are expected to be used as natural antioxidants.

Quality Characteristics of Jelly added with Peach(Prunus persica L. Batsch) Powder (복숭아 분말 첨가 젤리의 품질특성)

  • Lee, Jeong-Ae
    • Culinary science and hospitality research
    • /
    • v.22 no.3
    • /
    • pp.108-120
    • /
    • 2016
  • This study investigated the quality characteristics of jelly containing peach powder, which is well known for its various functions and biological activity. The feasibility of incorporating peach powder as a value-added food ingredient, using a model food system of jelly, was investigated. Peach powder was incorporated into jelly at 0, 5, 10, 15, and 20% levels. To analyze quality characteristics, pH of the jelly, spread factor, moisture content, color(L, a, b), hardness, DPPH radical scavenging activity, and sensory properties were measured. Qualities, such as pH content, color, hardness, and consumer preferences were determined. Lightness decreased, but redness and yellowness increased significantly (p<0.001). The antioxidative activity measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity of the jelly increased as the concentrations of peach powder increased. Lastly, the consumer acceptance test indicated that the highest level of incorporation (15%) had a considerable adverse effect on consumer preferences in all attributes. The jelly with 15% peach powder is recommended (with respect to overall preference score) for taking advantage of the functional properties of peach powder without sacrificing consumer acceptability.

Studies on Antioxidant Activity, Total Flavonoids and Polyphenols, and Reducing Power in Yakju with Different Ratios of Dandelion Root (민들레 뿌리를 첨가한 약주의 총 플라보노이드, 총 폴리페놀 함량과 전자공여능, 환원력 활성 조사)

  • Lee, Jong-Bok;Park, Hye-Kyong;Lee, Jong-Suk;Kim, Myung-Hee
    • Journal of the East Asian Society of Dietary Life
    • /
    • v.21 no.6
    • /
    • pp.882-887
    • /
    • 2011
  • We investigated the physicochemical and antioxidant activity characteristics of Yakju with different ratios (0, 5, 10, and 15%) of dandelion (Taraxacum officinale) root powder in order to find out potential as a functional fermented rice alcohol. Yakju samples were measured to obtain total flavonoid content, total polyphenol content, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, and reducing power. Total flavonoid and polyphenol contents increased with increasing amounts of dandelion root. The DPPH radical scavenging activity increased with the addition of dandelion root. Also, the reducing power was higher in Yakju with dandelion root compared with controls such as gallic acid, butylated hydroxytoluene, and ${\alpha}$-tocopherol. It is concluded that the Yakju fermented with additional dandelion root is functionally superior to the rice alcohol without dandelion root.