Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitation and Radical Scavenging Activity Evaluation of Iridoids and Phenylethanoids from the Roots of Phlomis umbrosa (Turcz.) using DPPH Free Radical and DPPH-HPLC Methods, and their Cytotoxicity

  • Le, Duc Dat (Drug Research and Development Center, College of Pharmacy, Catholic University of Daegu) ;
  • Nguyen, Duc Hung (Drug Research and Development Center, College of Pharmacy, Catholic University of Daegu) ;
  • Zhao, Bing Tian (Drug Research and Development Center, College of Pharmacy, Catholic University of Daegu) ;
  • Min, Byung Sun (Drug Research and Development Center, College of Pharmacy, Catholic University of Daegu) ;
  • Song, Si Whan (ChemOn Inc., Gyeonggi Bio-Research Center) ;
  • Woo, Mi Hee (Drug Research and Development Center, College of Pharmacy, Catholic University of Daegu)
  • Received : 2019.02.25
  • Accepted : 2019.04.17
  • Published : 2019.06.30
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Abstract

The roots of Phlomis umbrosa (Turcz.) (Phlomidis Radix) have been traditionally used to treat cold, reduce swelling and staunch bleeding. Four iridoids (1 - 3 and 5) and six phenylethanoid derivatives (4, and 6 - 10) were isolated from the roots of P. umbrosa. A simple, sensitive, and reliable analytical HPLC/PDA method was developed, validated, and applied to determine 10 marker compounds in Phlomidis Radix. Furthermore, the isolates were evaluated for cytotoxic and anti-oxidant activities as well as DPPH-HPLC method. Among them, compounds 4 and 6 - 9 displayed potent anti-oxidant capacities using DPPH assay with $IC_{50}$ values of $27.7{\pm}2.4$, $10.2{\pm}1.1$, $18.0{\pm}0.8$, $19.1{\pm}0.3$, and $19.9{\pm}0.6{\mu}M$, and compounds 6, 8, and 9 displayed significant cytotoxic activity against HL-60 with $IC_{50}$ values of $35.4{\pm}3.1$, $18.6{\pm}2.0$, and $42.9{\pm}3.0{\mu}M$, respectively.

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References

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