The purpose of this paper is to propose a method of rest to reduce work load of night shift workers for night shift work. The experiment was carried out 10minutes preparing time, 45minutes first work, 10minutes first rest, 45minutes second work, 10minutes second rest between 2 and 4 o'clock that the lowest physiological function of workers. The methods of rest set up as four patterns (1) non-action rest (2) non-action rest + listening music (3) action rest + non-action rest, (4) action rest + non-action rest + listening music. For the measurements of experiment, heart rates(R-R interval), critical flicker fusion frequency(CFF), blood pressure, oral temperature, reaction time and error rates were considered as criteria for work performance. As a result, action rest + non-action rest and action rest + non-action rest + listening music were more effective to reduce work load additional work than non-action rest and non-action rest + listening music.