A study was planned to see if the mammillary bodies have any influence upon orienting response Thirty-nine male rats were divided into two groups. One group of animals received electrolytic lesion of the mammillary bodies through stereotaxically implanted electrodes (mammillary body group), and the other group received the same treatment short of electrolytic lesion(operated control group). All animals were tested for orienting response 3 weeks after surgery. Orienting response was regarded as elicited when an animal in alertness ceased ongoing activity upon 1,000 Hz tone presentation and/or turned head toward the source of stimulus. Weak (momentary and inconspicuous) and strong (marked and more persistent) responses were discriminated. Occurrence and duration of orienting response were measured, and the rate of habituation was estimated in 20 trials. Shift of attention from sound to light stimulus (10 trials) as well as orienting response of thirsty animals to sound stimulus while drinking (5 trials) and while at rest (5 trials) were also tested. 1. Simple orienting respons to sound stimulus tended to occur more often and its duration tended to be shorter in the mammillary body group than in the operated control group. 2. With repetition of trials, the occurrence of orienting response in each trial decreased progressively in both groups (habituation). However, the rate of habituation was significantly less prominent in the mammillary body group than in the operated control group. 3. Under attention·shift situation, orienting response tended to occur less often and its duration tended to be shorter in the mammillary body group than in the operated control group. 4. Orienting response to sound stimulus while drinking to quench thirst tended to occur more often and its duration tended to be shorter in the mammillary body group than in the operated control group. In quiet state, however, there existed no group difference with regard to the occurrence and duration of the orienting response. Although no obvious inference can be drawn from the above results, they may suggest the mammillary bodies being involved in the orienting response as a control mechanism inhibitory to the occurrence and facilitatory to the habituation of the response.

This experiment was carried out to investigate systematically how the aerobic metabolic capacity of renal tissue reduced by the effects of a period of induced ischemia. Aerobic metabolic studies were performed on homogenates of cortex and medulla of rabbits. Ischemia was induced by occluding the renal vein or renal artery of the left kidney for an hour. The right kidney used as a paired control. Aerobic metabolism was asesssed by measuring the oxygen consumption using the Warburg's manometric apparatus. The results are summarized as follows: 1. One hour of occlusive ischemia does not increase in the kidney weight in the renal arterial occlusion but increase in the renal venous occlusion. 2. Occlusion of either the renal vein or renal artery for an hour did not reduce to any significant degree the level of endogenous substrate in cortical homogenates as measured the rates of $0_2$ consumption. 3. A significant reduction in the rate of $C_2$ consumption was noted in the medullary homogenates of renal venous occluded kidneys while renal arterial occlusion had less of an effect. 4. The capaciy of homogenates for aerobic metabolism is not reduced by acute ischemia, because of the higher rate of oxygen consumption induced by exogenous glucose in renal vein occlusion. 5. The oxygen consumption of medullary homogenate more decreased to acute ischemia than cortical homogenates. The results of this investigation suggest that one hour circulatory stasis does not reduce major potential capacity of renal cortical tissue at the subcellular level to produce energy. In contrast, the aerobic metabolism of medullary tissue is reduced by renal ischemia. Further, both cortex and medulla appear to be more sensitive to ischemia induced by renal venous occlusion than by renal arterial occlusion.

인삼주정추출물이 마우스의 대뇌조직 핵산 함유량에 어떤 영향을 미치는지를 알기위하여 30마리의 마우스($18{\sim}20\;gm)$ 수컷을 인삼군과 식염수군으로 나우어 다음과 같은 실험을 하였다. 인삼군에서는 몸 무게 10gm에 대하여 인삼주정추출액(생리적 식염수 1ml 속에 4mg의 인삼주정추출물이 포함된 용액)을 0.05ml의 비율로 매일 등뒤 피하에 5일 동안 주사하였으며, 식염수군에는 생리적 식염수를 몸 무게 10gm에 대하여 0.05ml의 비율로 인삼군에서 한 것과 동일한 방법으로 주사하였다. 인삼추출액 혹은 식염수 투여가 시작된지 제 5일째 되는 날에는 해당 약물을 투여한 2시간 후에 동물을 도살하여 대뇌조직을 적출하고, 이 조직의 핵산함유량을 Schmidt-Thannhauser-Schneider의 화학적 정량법을 이용하여 측정하였다. 이들 측정치를 지표로 하여 인삼이 대뇌조직 핵산에 미치는 영향을 관찰한 바 다음과 같은 결과를 얻었다. 1. 인삼군의 대뇌조직 RNA 및 DNA 함유량은 식염수군의 그것 보다 현저하게 많았다. 2. 인삼군의 대뇌조직 RNA/DNA 비율은 식염수군의 그것에 비하여 현저하게 적었다. 이는 인삼군에서 RNA 보다 DNA의 증가가 더욱 현저하였기 때문이다. 위의 결과로 미루어 보건데 인삼은 대뇌조직 RNA 및 DNA 함유량을 유의하게 증가시킨다고 추리된다.

Contrary to most of European and American investigators failed to find out the seasonal variations of basal metabolic rate in man, Japanese and Korean investigators reported the increase in winter, decrease in summer season. But the causes of variation were not found clearly. To find out whether metabolic acclimatization to climate could be arise or not in human being, the basal metabolic rate was determined monthly for a period of one year in Airmen volunteers who live in Seoul, with 9 l Collins spirometer. The results obtained were as follows: 1. The average ambient temperature was lowest in February $(-5.88^{\circ}C)$ and highest in July $(27.34^{\circ}C)$. 2. Basal metabolic rate was lowest in June and highest in December showing seasonal variations. Interestingly, the increase of basal metabolic rate followed after the drop of ambient temperature below $0^{\circ}C$ (December) and the decrease followed after the elevation of ambient temperature from optimum to hot (June) or cold to warm (March). 3, Mean skinfold thickness increased in spring, decreased in winter. 4. These findings indicate that the basal metabolic rate of Korean reveals the seasonal variation affected by ambient temperature highly.

The right cervical vagus nerve was electrically stimulated for 30 sec, and 30 minutes recording cardiac rate responses and electrocardiogram. The main purposes of the present experiment are to determine effect of stimulation frequency on the maintenance of cardiac rate responses and to determine recovery time of sinus rhythm after asystole period followed by idioventricular rhythm during prolonged electrical stimulation of the vagus, and the optimal stimulation parameters for vagal stimulation were studied as well. The results obtained are summarized as follows: 1. The maximum negative chrontropic responses were obtained with the following ranges of electrical parameters. Intensity: 3V-7V, Frequency: 20/sec-60/sec, and pulse duration: 5 msec-20 msec. 2. Compared with the responses from sympathetic effectors, cardiac rate responses to electrical stimulation of vagus nerve were well maintained with all stimulation frequencies. 3. At all stimulation frequencies except 20/sec, sinus node started to take over primary pacemaker activity when cardiac rates were restored to about 38-40/min. 4. It was indicated that upper limit of idioventricular rhythm does not exceed 38-40/min. 5. With the stimulation parameter set of 20/sec-5 msec-3 V, sinus rhythm did not appear during 30 minutes of stimulation period. Therefore, this electrical parameter set appears to be optimal for elicitation of prolonged and maximum cardiac rate responses by vagal stimulation.

Two polyethylene tubes were inserted into the esophagus of anesthetized rabbit in order to record the fluctuation of the intraluminal pressure through the orifices located near the tips of the tubes. The orifice of the first tube was 10 cm apart from the incisor of the rabbit and the orifice of the second tube was 5 cm below that of the first one. The tubes were filled with saline solution running at various rates ranging from 1.5 ml/min. to 4.2 ml/min. The tubes were connected to the pressure transducers and the electrical signals were recorded by the physiograph. When the peristaltic wave approached to the orifice a rise in the pressure was recorded, returning to the base line when the portion of the orifice was quiescent. The frequency of the peristaltic motion and the velocity of the wave were studied in connection with the flow rate of saline solution through the tubes and in the case of massive acute hemorrhage. The results obtained were as follows: 1. There was reflux of fluid induced during the procedure of the experiment. This outwrad flow through the pharynx seemed to elicite swallowing reflexes. Accordingly, the frequency of peristalsis of the esophagus was largely dependent on the flow rate of the fluid through the inserted tubes. By the flow rate of 1.5 ml/min., 2.5 ml/min., or 4.2 ml/min., the frequencies of the peristalsis were revealed to be $8.6{\pm}3.6/10min.,\;14.5{\pm}4.8/10min.\;or\;21.1{\pm}6.3/10min.,$ respectively. The velocity of peristalsis also coincided with the enhanced motility of the esophagus, showing $6.6{\pm}1.5\;cm/sec.,\;8.9{\pm}3.9\;cm/sec.,\;or\;12.4{\pm}4.6\;cm/sec.,$ respectively. 2. By acute hemorrhage, amounting to 2% of the body weight, the frequency of the peristalsis increased to twofold of the control and the propagation velocity also increased by 52 percent. 3. Retransfusion of the shed blood resulted in divergent responses. In some cases there were noticable ameliorations of the effects brought by acute hemorrhage, and in the others there were still increasing tendenies of the motility after the transfusion. 4. Some speculation was made about the possibility of a kind of relationship between the irreversibility of the hemorrhagic shock and the absence of responses by transfusion. 5. The peristalsis persisted even after complete disconnection at the midportion of the esophagus, reaffirming the view of a central regulation of the spatiotemporally coordinated motility, peristalsis.

This experiment was designed to explore specific functional relationship between the vestibular canals and the extraocular oblique muscles by observing the isometric tension responeses of the muscles to the selected vestibular canal excitation. The vestibular excitation was simulated by either stimulation of the individual canal nerve or endolymphatic fluid displacement in each canal. Each canal nerve was subjected to square wave pulses with a monopolar wire electrode placed closely to the ampullary nerve endings for electrical stimulation, and a fine stainless cannula was introduced into the each canal toward the ampulla and a minute amount $(0.5{\sim}3.5\;microliter)$ of fluid was injected in or ejected out by means of a microsyringe connected to the cannula to produce ampullopetal or ampullofugal displacement of endolymphatic fluid. The superior oblique muscle was contracted by the excitation of homolateral canals and was relaxed by contralateral canals. On the contrary, the inferior oblique was contracted by the contralateral canals and was relaxed by the homolateral canals. Summation of excitatory and inhibitory canal effects from the bilateral vestibular system was demonstrable on the tension changes of the oblique muscles. Excitation of either dual or triple canals of the unilateral vestibular system also caused summation effect on the tension response of the oblique pair; thus multiple signals from the different ampullary receptors seems to be converged into the relevant ocular motor muclei. Since the superior and inferior obliques are known to receive their motor fibers from the contralateral trochlear nuclei and intermediate nuclei of the homolateral oculomotor complex respectively, the above experimental evidences indicate that the ocular motor nuclei for oblique muscles receive excitatory signals from the contralateral vestibular canals and inhibitory signals from the homolateral canals.

In an attempt to better understand the radioprotective effect of reduced glutathione(GSH), and to observe a possible radioprotective effect of Ginseng extract, whole body X-irradiation of 1,200 r was administered to the mouse either independently or immediately following the injection of GSH or Ginseng extract to the mouse intraperitoneally. The non-protein sulfhydryl (NP-SH) and non-protein disulfide (NP-SS) levels of the liver, and NP-SH level of NP-SH of the blood of the mouse were measured at 30, 60 and 120 minutes, and results were compared with the normal. The results thus obtained are summarized as follows; 1) The normal values of NP-SH and NP-SS of the mouse liver were $5.90{\pm}0.46\;{\mu}\;mol/gm\;wet\;wt.,\;and\;3.02{\pm}0.42\;{\mu}\;mol/ml$ wet wt., respectively, and the normal value of NP-SH of NP-SH of the mouse blood was $3.98{\pm}1.29\;{\mu}\;mol/ml$ 2) The injection of both GSH and Ginseng extract produced the highest values of NP-SH in the liver at 30 minutes, but a gradual decrease to the normal was observed thereafter. When X-irradiation alone was applied, the liver NP-SH value was lower than the normal at 60 minutes post-irradiation and thereafter. When Ginseng extract was injected immediately prior to X-irradiation, the liver NP-SH was lower than the normal throughout the experiment with the lowest value at 60 minutes. However, the combination of GSH and X-irradiation produced higher than the normal values throughout the entire experiment. 3) The liver NP-SS value was most significantly elevated at 30 minutes after the injection of GSH, hut the recovery to the normal was observed thereafter. The injection of Ginseng extract produced slightly higher liver NP-SS values at 30 and 60 minutes, but the value at 120 minutes was similar to the normal. The single application of X-irradiation resulted in the lower then normal liver NP-SS values throughout the entire experiment. When GSH was injected price to X-irradiation, the liver NP-SS values were higher than the normal at 30 and 60 minutes followed b the recovery to the normal at 120 minutes. The combination of Ginseng extract and X-irradiation showed generally lower liver NP-SS values throughout the experiment. 4) The blood NP-SH showed the higher than the normal values in all the experimental groups except when GSH was injected prior to X-irradiation alone produced e significantly elevated blood NP-SS value at 30 minutes post-irradiation.