Four kinds of Bupleuri Radix, namely 'Dang shiho', 'Won shiho', 'Shik shiho' and 'Jook shiho', are currently used in the market of Korea. It is thought that 'Dang shiho' is the root of Bupleurum falcatum and B. scorzoneraefolium. It was also reported that 'Won shiho' and 'Shik shiho' are the root of B. falcatum. Since 'Jook shiho' which is widely used in the market and even exported, has not been clarified as to the origin of the plant and the chemical components, the author obtained some results by this study, the summaries of which are as following: 1. The results of microscopical examination of 'Jook shiho' and 'Kae shiho' were entirely identical and the TLC patterns were coincided as well. It was therefore definitely clarified that the origin of 'Jook shiho' is Bupleurum longe-radiatum. 2. The TLC patterns of 'Won shiho', 'Jook shiho' and 'Kae shiho' which are belonging to the same family and the same genus, appeared identical, and reveal the similarity of the chemical constituents and thereby strongly support the fact that 'Jook shiho' can be used for the same purpose as 'Won shiho'.