Many advanced scholars and policy makers say that biotechnology is and will be the most important technology in 21th century. And biotechnology has a lots of character which are new and high tech industry, costing too much on early R&D stage and so on. One of them is that it can't be developed all seater by one single firm. So effective and efficient way to solve this generic problem is using the infrastructure which is given by government for flourishing the bio industry, especially, Knowledge infrastructure. The purpose of this study is to understand and give the information about which knowledge infrastructure affects biotechnology firms to obtain competitive advantage in technological improvement. To develop this object, I made 5 variables about infrastructure in biotechnology such as high-skilled researchers infrastructure, R&D policy infrastructure, industrialization infrastructure that supports the biotechnology industry, networking infrastructure between firms, universities, research centers, and finally bio-safety infrastructure. According to this study, to obtain technological competitive advantage for Korean bio firms, all main actors those are government, industry, university, research center they try to develop bio sector at the same time. After this cooperated effort, Korean bio firms and industry can get a international competitiveness in the global society which no one conquer the market.