Food Science and Biotechnology
Korean Society of Food Science and Technology (KOSFOST)
- Monthly
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- 1226-7708(pISSN)
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- 2092-6456(eISSN)
Domain
- Agriculture, Fishery and Food > Science of Food and Crops
- Agriculture, Fishery and Food > Food Science
Aim & Scope
The Food Science and Biotechnology (Food Sci. Biotechnol.; FSB) was launched in 1992 as the Food Biotechnology and changed to the present name in 1998. It is an international peer-reviewed journal published monthly by the Korean Society of Food Science and Technology (KoSFoST). The FSB journal covers; Food chemistry/food component analysis Food microbiology and biotechnology Food processing and engineering Food hygiene and toxicology Biological activity and nutrition in foods Sensory and consumer science s Consumer perception and sensory evaluation on processed foods are accepted only when they are relevant to the laboratory research work. As a general rule, manuscripts dealing with analysis and efficacy of extracts from natural resources prior to the processing or without any related food processing may not be considered within the scope of the journal. The FSB journal does not deal with only local interest and a lack of significant scientific merit. The main scope of our journal is seeking for human health and wellness through constructive works and new findings in food science and biotechnology field.
http://www.fsnb.or.kr/submission/ KSCI KCI SCOPUS SCI SCIEVolume 16 Issue 2
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Fresh sprouts such as alfalfa, mung bean, radish, broccoli, and soybean sprouts have become very popular due do their high nutritional value. However, there have been several outbreaks of illness in the last few years that have been attributed to sprout consumption. A number of methods have been used to improve the safety of seed sprouts. One promising technology is the use of ionizing radiation treatment. Irradiation with doses up to 8 kGy has been approved in the USA to control microbial pathogens in seeds intended for sprout production. This review focuses on the potential use of ionizing radiation in reducing the pathogen levels in seed sprouts. The effects of irradiation on seed germination and the nutritional quality of the sprouts are discussed.
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The beneficial effects of fruits, vegetables, and beverages on human health have been attributed to their antioxidant activities. Therefore, antioxidant activity of food products is recognized as one of the important parameters in determining their functional values. Until now, antioxidant activity has been measured by various chemical and biological methods; however, many factors confound the reliability and reproducibility of measurements of antioxidant activity of food. In vitro methods may provide a useful indication of antioxidant activity but their results may not translate to the human biological system, while in vivo tests are difficult to carry out due to the intricate processes of uptake, cellular transportation, and metabolism of individual antioxidant components. Therefore, as long as these limitations exist, our best option is to measure the antioxidant activity in food directly. This review briefly summarizes currently available methods for the measurement of antioxidant activity in food and examines their respective validity.
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The type of mixed gel network of
$\kappa$ -carrageenan/agar was determined by applying rheological principles. Apparent Young's modulus of the mixed gels was mathematically analyzed with (a) simply adding the moduli of two component gels, (b) phase-separated type's upper and lower bound models, (c) interpenetrating type's logarithmic model. The experimental data fitted the estimates from the operation (a). Whereas, as for the models (b), the experimental values in the agar-rich region fitted the estimates of the upper bound model, but in the$\kappa$ -carrageenan-rich region slightly deviated from those of the lower bound model. It reflected an evidence of a phase-separated type, although it was not typical, that there must be data good-fit in the agar-rich and$\kappa$ -carrageenan-rich regions with the upper and lower bound models, respectively. Experimental values disagreed with estimates of the model (c). Gel time was analyzed to evince the phase-separated type. As agar concentrations increased at a fixed amount of$\kappa$ -carrageenan, gel time gradually decreased and then sharply increased and decreased again. The pattern of such change in gel time also represented a typical behavior of phase-separated type's mixed gels. -
The objectives of this study were to examine changes in the color and antioxidant compounds of paprika powder under various conditions, as well as to establish the suitable conditions for drying and storage. Paprika was dried using the following methods: freeze-drying, vacuum drying, far infrared-ray drying, and hot-air drying. Measurements of the moisture content, color pigments, and antioxidant compounds (total carotenoids, capsanthin, ascorbic acid, and total polyphenols) were completed during 120 days of storage at 4 and
$30^{\circ}C$ . We found that drying methods, storage temperatures, and packaging materials affected the American Spice Trade Association (ASTA) and Hunter color values, as well as the antioxidant content of paprika powder. There was a high correlation (r=0.87, p<0.01) between the ASTA color and the$a^*/b^*$ value. The loss of red color was closely related to the reduction of moisture content (r=0.81, p<0.01) during storage. Drying paprika with a low temperature in the absence of air resulted in better retention of the carotenoids and ascorbic acid. Also, as the retention of the carotenoids and ascorbic acid increased, the stability of the red pigment increased. Freeze-drying was found to be the most suitable drying method for the stability of the antioxidant compounds and red pigment. -
In order to investigate the effects of Pichia farinosa SKM -1, Pichia anomala SKM-T, Galactomyces geotrichum SJM-59, and Saccharomyces cerevisiae on extending the shelf life of kkakdugi, 4 kinds of lyophilized yeasts adding kkakdugis were prepared and stored at
$4^{\circ}C$ for 30 days. Except S. cerevisiae adding group, 3 kinds of yeast adding groups were maintained their desirable levels (ca. pH 4.2 and 0.6% acidity) during the fermentation. The hardness of yeast adding groups was higher than those of control during the experiments. The number of yeast and the ratio of lactic acid against to total bacteria in P. farinosa SKM-1, P. anomala SKM-T, and G geotrichum SJM-59 adding groups were lower than that of control and/or S. cerevisiae adding group. Based on acidity, kkakdugi made with P. farinosa SKM-1, P. anomala SKM-T, and G geotrichum SJM-59 remained edible about 10 days longer than the control product. -
The objectives of this study were to determine the quality characteristics, antioxidant activity, and cytotoxicity of fermented ginseng wine at each fermentation step. In the first mash with and without ginseng, viable cell counts (total cell, lactic acid bacteria, and yeast) were maximum between 2 to 4 days of fermentation. At the beginning of fermentation, Brix and ethanol contents, and titratable acidity increased, while pH decreased rapidly. At 3 days of fermentation of the second mash with ginseng, the viable cell counts were similar to those without ginseng and then continually decreased. At the end of fermentation, the pH of the second mash with ginseng was 4.00, lower than the pH of the second mash without ginseng, which was 4.35. Alcohol contents of second mashes with and without ginseng were 12.2 and 11.8%, respectively. In the aging period of ginseng and rice wines, the pH, titratable acidity, Brix, and ethanol contents did not change markedly. The results of sensory evaluation showed that fermented ginseng wine had good flavor and high acceptability. In the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity assay, fermented ginseng wine (
$IC_{50}$ : 0.394 mg/mL) showed higher antioxidant activity than fermented rice wine ($IC_{50}$ : 0.884 mg/mL). The butanol fraction of fermented ginseng wine exhibited weak cytotoxic activity against P388 and HeLa cell lines. -
Park, Ji-Young;Park, Chung-Mu;Kim, Jin-Ju;Noh, Kyung-Hee;Cho, Chung-Won;Song, Young-Sun 205
This study was designed to investigate the suppressive effect of chlorophyll a on nitric oxide (NO) production and intracellular oxidative stress. In addition, chlorophyll a regulation of nuclear factor (NF)${\kappa}B$ activation and inducible NO synthase (iNOS) expression were explored as potential mechanisms of NO suppression in a lipopolysaccharide (LPS)-stimulated macrophage cell line. RAW 264.7 murine macrophages were preincubated with various concentrations ($0-10\;{\mu}g/ mL$ ) of chlorophyll a and stimulated with LPS to induce oxidative stress and inflammatory response. Treatment with chlorophyll a reduced the accumulation of thiobarbituric acid-reactive substances (TBARS), enhancing glutathione level and the activities of antioxidative enzymes including superoxide dismutase, catalase, glutathione peroxidase (GSH-px), and glutathione reductase in LPS-stimulated macrophages compared to LPS-only treated cells. NO production was significantly suppressed in a dose-dependent manner (p<0.05) with an$IC_{50}$ of$12.8\;{\mu}g/mL$ . Treatment with chlorophyll a suppressed the levels of iNOS protein and its mRNA expression. The specific DNA binding activities of NFkB on nuclear extracts from chlorophyll a treated cells were significantly suppressed in a dose-dependent manner with an$IC_{50}$ of$10.7\;{\mu}g/mL$ . Chlorophyll a ameliorates NO production and iNOS expression through the down-regulation of NFkB activity, which may be mediated by attenuated oxidative stress in RAW 264.7 macrophages. -
Fucoidan (fucan sulfate) is a fucose-containing sulfated polysaccharide from brown algae such as Fucus vesiculosus, Ecklonia kurome, and Cladosiphon okamuranus. The aim of this study was to investigate the effect of fucoidan on the expression of diabetes-related genes in mouse cell line 3T3-L1. 3T3-L1 adipocytes were cultured for 48 hr with or without fucoidan (10, 100, and 500 ppm) on a 60 mm dish. Reverse transcription polymerase chain reaction (RT-PCR) was used for measurement of peroxisome proliferators activated receptor
${\gamma}\;(PPAR{\gamma})$ , CCAAT/enhancer binding protein${\alpha}\;(C/EBP{\gamma})$ , and glucose transporter 4 (GLUT4) RT-PCR analysis revealed that expression level of GLUT4,$PPAR{\gamma}$ , and$C/EBP{\alpha}$ mRNAs increased with fucoidan treatment from 10 to 500 ppm in a dose-dependent manner. Fucoidan appears to enhance insulin sensitivity by increasing the expression level of diabetes-related genes in 3T3-L1 adipocytes. Therefore, fucoidan is potentially useful as a natural therapeutic material for hyperglycemia in type II diabetes patients. -
The anticoagulant properties of Cinnamomum cassia bark-derived materials were evaluated against platelet aggregation induced by arachidonic acid (AA), collagen, platelet activating factor (PAF), or thrombin, and these effects were then compared to those of three commercially available compounds (cinnamic acid, cinnamyl alcohol, and aspirin). The active constituent obtained from C. cassia barks was isolated by silica gel column chromatography and high pressure liquid chromatography (HPLC), and was characterized as trans-cinnamaldehyde by MS,
$^1H-NMR$ ,$^{13}C-NMR$ , and IR spectroscopy. With regard to 50% inhibitory concentration ($IC_{50}$ ) values, cinnamaldehyde was found to effectively inhibit platelet aggregation induced by AA ($IC_{50},\;43.2\;{\mu}M$ ) and collagen ($IC_{50},\;3.1\;{\mu}M$ ). By way of comparison, cinnamaldehyde proved to be a significantly more potent platelet inhibitor against platelet aggregation induced by collagen than aspirin. The effect exerted by cinnamaldehyde against platelet aggregation induced by AA was 1.2 times less than that of aspirin. These results indicate that cinnamaldehyde may prove useful as a lead compound for the inhibition of platelet aggregation induced by AA and collagen. -
Ground garlic inhibited the cross-linking reaction of myosin and incorporation of monodansylcadaverine (MDC) in salted Alaska pollack surimi catalyzed by transglutaminase (TGase). The component responsible for the inhibition was a thermostable, low molecular weight compound. The component also inhibited microbial transglutaminase (MTGase). The inhibition by garlic was reversibly recovered upon addition of 2-mercaptoethanol. The inhibitory component was therefore hypothesized to contain sulfhydryl groups within its structure. Alliin itself did not inhibit the cross-linking reaction. However, the addition of alliin together with garlic increased the inhibition. This result suggested that compounds derived from alliin was responsible for the inhibition of TGase activity.
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This study evaluated the effects of carnitine supplementation on obesity caused by a high-fat diet in C57BL/6J mice. The mice were fed a normal diet (ND), high-fat diet (HD), or carnitine-supplemented (0.5% of diet) high-fat diet (HDC) for 12 weeks. The results showed that body weight, energy intake, and feed intake were lower in the HDC group than the control groups. Acid-soluble acylcarnitine (A SAC), acid-insoluble acylcarnitine (AIAC), and total carnitine (TCNE) in the serum and liver were significantly higher in the HDC group. Hepatic carnitine palmitoyl transferase-I activity was significantly higher in the HDC group than the control groups. Acyl-coA synthetase (ACS) and carnitine palmitoyl transferase-I (CPT-I) mRNA expression in the liver was highest in the HDC group, however hepatic acetyl-coA carboxylase (ACC) mRNA expression in this group was lowest. Serum leptin levels and abdominal fat weight were lowest in the HDC group. We concluded that L-carnitine supplementation diminished the risk of obesity caused by a high-fat diet.
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Water resistance of three biopolyester films, such as poly-L-lactate (PLA), poly-hydroxybutyrate-co-valerate (PHBV), and Ecoflex, and low density polyethylene (LDPE) film was investigated by measuring contact angle of various probe liquids on the films. The properties measured were initial contact angle of water, dynamic change of the water contact angle with time, and the critical surface energy of the films. Water contact angle of the biopolyester films (
$57.62-68.76^{\circ}$ ) was lower than that of LDPE film ($85.19^{\circ}$ ) indicating biopolyester films are less hydrophobic. The result of dynamic change of water contact angle also showed that the biopolyester films are less water resistant than LDPE film, but much more water resistant than cellulose-based packaging materials. Apparent critical surface energy for the biopolyester films (35.15-38.55 mN/m) was higher than that of LDPE film (28.59 mN/m) indicating LDPE film is more hydrophobic. -
Park, Shin-Young;Choi, Jin-Won;Chung, Duck-Hwa;Kim, Min-Gon;Lee, Kyu-Ho;Kim, Keun-Sung;Bahk, Gyung-Jin;Bae, Dong-Ho;Park, Sang-Kyu;Kim, Kwang-Yup;Kim, Cheorl-Ho;Ha, Sang-Do 238
Square root models were developed for predicting the kinetics of growth of Listeria monocytogenes in sesame leaves as a function of temperature (4, 10, or$25^{\circ}C$ ). At these storage temperatures, the primary growth curves fit well ($R^2=0.898$ to 0.980) to a Gompertz equation to obtain lag time (LT) and specific growth rate (SGR). The square root models for natural logarithm transformations of the LT and SGR as a function of temperature were obtained by SAS's regression analysis. As storage temperature ($4-25^{\circ}C$ ) decreased, LT increased and SGR decreased, respectively. Square root models were identified as appropriate secondary models for LT and SGR on the basis of most statistical indices such as coefficient determination ($R^2=0.961$ for LT, 0.988 for SGR), mean square error (MSE=0.l97 for LT, 0.005 for SGR), and accuracy factor ($A_f=1.356$ for LT, 1.251 for SGR) although the model for LT was partially not appropriate as a secondary model due to the high value of bias factor ($B_f=1.572$ ). In general, our secondary model supported predictions of the effects of temperature on both LT and SGR for L. monocytogenes in sesame leaves. -
A biochemical characterization of the tannases from Paecilomyces variotii (produced at Unicamp), Aspergillus niger (purchased from Industrial Kerry Bio-Science) and A. niger cnpat 001 (purchased from Embrapa Agroindustrial Tropical-Brazil) was carried out. P variotii is a new strain obtained from the screening of 500 fungi that were tested for their production of tannase. The biochemical properties of this new tannase from P variotii were determined and compared with those of two other tannase preparations. The tannase produced from P. variotii showed optimum activity at pH 6.5. The functional temperature range of the tannases was from
$20-70^{\circ}C$ , with optima at$70^{\circ}C$ for P. variotii and at$60^{\circ}C$ for the commercially obtained tannase, whereas A. niger cnpat 001 showed optimum activity at$40^{\circ}C$ . The effects of 1 mM preparations of cations and anions, inhibitors, surfactants, and chelators on the tannase activity from P. variotii were also studied. -
Kim, Hag-Lyeol;Son, Yeon-Hee;Kim, Seon-Jae;Kim, Du-Woon;Ma, Seung-Jin;Cho, Geon-Sik;Kim, In-Cheol;Ham, Kyung-Sik 249
The effects of chronic chitosan salt supplementation on the systolic (SBP) and diastolic blood pressure (DBP), and physiological parameters were investigated in healthy male and female adult. Chitosan salt was conducted by measuring various health-related factors such as body composition, plasma$Na^+$ ,$Cl^-$ , lipid, and lipoproteins profiles, glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) activity. Chitosan salt supplementation no significant differences before and after supplement in body composition variables and in SBP and DBP in either male or female. Plasma sodium and chlorine concentration no significant changes during chitosan salt supplementation, and no significant difference between two genders. Plasma GOT and GPT activity no different before and after supplement in either male or female. GOT activity significantly higher for male before supplement (p<0.05), and 2 weeks after supplement (p<0.01). The lipid and lipoproteins profiles of plasma no significant changes during chitosan salt supplementation in either male or female subjects. In summary, the chronic intake of chitosan salt did not affect the SBP or DBP, and posed no health risks. -
The effects of temperature heating-up rate and pressure building-up phase on the inactivation of Listeria innocua ATCC 33090 were evaluated in buffered peptone water. The number of L. innocua was reduced by 5.57 and 6.52 log CFU/mL during the nonisothermal treatment (the come-up time followed by isothermal process) and the isothermal treatment, respectively, at
$60^{\circ}C$ . When compared to the isothermal treatment (0.76$33.2^{\circ}C/min$ of temperature heating-rate. The effect of the combined high pressure and thermal processing on the inactivation of L. innocua increased with increasing pressure and temperature. At all temperature levels from 40 to $60^{\circ}C$ under 700 MPa, L. innocua was not detected by enrichment culture (>7 log reduction). -
Gleditsiae Semen (GS) has been used in both Korea and China as herbal medicine for the treatment of cephalalgia, catharsis, and other diseases. However, the apoptosis of GS against human cancer cells has not previously been investigated. The primary objective of this study was to determine the mechanisms inherent in GS-induced cytotoxicity and apoptosis, using methanolic extract of GS (GSE) in HT-29 human colon carcinoma cells. We found that GSE induced cytotoxicity in HT-29 cells in a dose-dependent manner, and this effect was verified via a lactate dehydrogenase release assay and a colony formation assay. In particular, HT-29 cells showed extensive cell death when treated with
$50\;{\mu}g/mL$ of GSE; the calculated$IC_{50}$ value was$20\;{\mu}g/mL$ . It induced characteristic apoptotic signs in HT-29 cells, including chromatin condensation and DNA fragmentation, occurring within 6-24 hr when the cells were treated at a concentration of$50\;{\mu}g/mL$ . Interestingly, we detected the activation of caspase-3 and -9, but not caspase-8, and apoptotic bodies in GSE-treated HT-29 cells. Collectively, our results indicate that GSE induces apoptosis via a mitochondria-mediated apoptotic pathway, and these findings may be significant with regard to the development of a new drug for the treatment of human colon carcinoma cells. -
There is significant interest in finding new sources of acetylcholinesterase (AChE) inhibitors for use in treating Alzheimer's disease, since only a few AChE inhibitors are available for clinical use, such as galanthamine, physostigmine, and tacrine. The ethanol extract of Chrysanthemum indicum Linne flowers was analyzed and found to markedly decrease AChE activity. Acaciin and
$acacetin-7-O-{\beta}-D-galactopyranoside$ were identified as the active compounds responsible for the AChE inhibition by using an activity-guided fractionation strategy. The relationship between structure and activity for five flavonoids (acaciin,$acacetin-7-O-{\beta}-D-galactopyranoside$ , luteolin, and two other commercially available flavonoids, i.e., apigenin and acacetin) was also investigated, revealing that the presence of methoxy groups at C-4' in the B ring and a sugar at O-7 in ring A appear to be essential for the inhibition of AChE. -
To characterize the polysaccharides which exist as soluble forms in fruit wines, crude polysaccharides were isolated from red, white, raspberry, wild grape, and pear wine, respectively. Among them, the crude polysaccharide (RW-0) in red wine showed the highest yield and considerable amounts of thiobarbituric acid (TBA)-positive materials. The pectic polysaccharide RW-2 was purified to homogeneity from RW-0 by subsequent size-exclusion chromatography using Sephadex G-75 and its structure was characterized. RW-2 consisted of 14 different monosaccharides which included rarely observed sugars in general polysaccharides, such as 2-O-methyl-fucose, 2-O-methyl-xylose, apiose (Api), 3-C-carboxy-5-deoxy-L-xylose (aceric acid, AceA), 3-deoxy-D-manno-2-octulosonic acid (Kdo), and 3-deoxy-D-lyxo-2-heptulosaric acid (Dha). Methylation analysis indicated that RW-2 comprised at least 20 different glycosyl linkages such as 3,4-linked fucose, 2,3,4-linked rhamnose, 3'-linked apiose, and 2,3,3'-linked apiose, being characteristic in rhamnogalacturonan II (RG-II). High performance size-exclusion chromatography indicated that RW-2 mainly comprised RG-II of higher molecular weight (12,000), and that the changes of molecular weight to apparent 7,000 under less than pH 2.0 were observed. These analyses indicated that the higher molecular weight polysaccharide in RW-2 was mainly present as a RG-II dimer.
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The effect of using Korean wheat flour versus Australian wheat flour on noodle quality as a result of differing physical and chemical properties of the flours was investigated. The results provided appropriate technical information for selection of wheat varieties to produce high quality Korean salted noodles. Noodle quality was quantified based on measurement of the appearance and texture of noodles. When consumer preference tests were conducted, a firmer and more elastic texture was preferred for Korean white salted noodles, however, when appearance was included in the consumer tests, noodles made with Australian wheat were favored over Korean wheats. Korean flour was found to produce firmer and more elastic noodles, whereas Australian flour produced brighter, creamier colored noodles. In flour quality tests, Korean flours were found to have a higher setback viscosity and lower swelling power than Australian flour. Additionally, Korean flours had higher water absorption values. Protein content of flour was an important parameter affecting the firmness of Korean noodles, whereas setback viscosity and swelling power were the major determinants of elasticity. Overall, the important parameters for determination of the quality of Korean salted noodles were high setback viscosity, low swelling power, and high protein content.
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Han, Sung-Tai;Shin, Cha-Gyun;Yang, Byung-Wook;Hahm, Young-Tae;Sohn, Uy-Dong;Im, Byung-Ok;Cho, Soon-Hyun;Lee, Boo-Yong;Ko, Sung-Kwon 281
The objective of this research is to provide basic information necessary to differentiate between ginseng (Panax ginseng) grown in woods environments and cultivated ginseng. The ginseng saponin (ginsenoside) contents of Korean woods-grown, 4 year-old cultivated, and 6 year-old cultivated ginsengs were determined via HPLC analysis. The total saponins in the woods-grown ginseng (0.648%) were approximately twice that of the 4 year-old cultivated (0.270%) and the 6 year-old cultivated ginsengs (0.280%). The protopanaxadiols (PD)/protopanaxatriols (PT) ratio of the woods-grown ginseng (3.258%) was higher than that of the 4 year-old cultivated (2.456%) and the 6 year-old cultivated ginsengs (2.183%). The$Rb_1/Rg_1$ ratio of the woods-grown ginseng (10.225%) was also higher than those of the 4 year-old cultivated (3.514%) and the 6 year-old cultivated ginsengs (4.865%). -
Heo, Seok;Hettiarachy, Navam;Park, Jong-Seok;Kim, Hyung-Il;Paik, Hyun-Dong;Yun, Mi-Suk;Lee, Si-Kyung 285
A 3% suspension of heat-stabilized defatted rice bran was treated with papain, followed by inactivating the enzyme by heat, and centrifuged. The supernatant was subjected to ultrafiltration, and fractions with various molecular sizes, F1 (>30 kDa), F2 (10-30 kDa), F3 (5-10 kDa), F4 (3-5 kDa), and F5 (3 kDa<), were freeze-dried, and evaluated for antimutagenicity by Ames test using Salmonella typhimurium TA 100 against phenazine methosulfate. The F3 fraction containing highest antimutagenicity from ultrafiltration was separated into 6 fractions by DEAE-Sephadex A-25 ion-exchange column chromatography (F3-1-F3-6). Each fractions having protein contents were pooled, dialyzed, freeze dried, and evaluated for antimutagenicity. Among the six fractions, the F3-1, F3-2, and F3-6 fractions showed antimutagenicity, which were 80.2, 53.4, and 58.6% at concentration of$100\;{\mu}g/plate$ , respectively. These F3-1, F3-2, and F3-6 fractions were subjected to Sephadex G-50 gel filtration column chromatography for further purification. Among the purified fractions, the F3-1-1, F3-2-2, and F3-6-1 fractions showed antimutagenicity of 84.5, 58.6, and 69.8% at concentration of$100\;{\mu}g/plate$ , respectively. It is thought that these peptides can find application for nutraceutical and pharmaceutical products. -
Cassumunar ginger (Zingiber montanum Roxb.) was grown in the experimental field at the Department of Horticulture, Kasetsart University, Thailand. The antioxidant activity and volatile oil content of rhizomes of varying age were measured. Antioxidant activity as determined using the DPPH (diphenyl-2-picrylhydrazyl) method differed significantly between samples of different ages. Antioxidant activity and rhizome age were positively correlated, with 22-month old rhizomes showing the highest radical scavenging activity (79.19%). Volatile oil was obtained by steam distillation of fresh rhizomes. The extraction yield of volatile oil was highest in l6-month old rhizomes (13.02 mL/kg). GC-FID data indicated the presence of three major compounds, sabinene, terpinen-4-ol and (E)-1-(3',4'-dimethylphenyl) butadiene (DMPBD), however none of the major components were correlated with the age of rhizome.
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The actinidin (EC 3.4.22. 14) found in kiwifruit is a cysteine protease. In order to obtain the actinidin gene from the Chinese wild kiwifruit, primers were designed on the basis of the actinidin gene of Actinidia deliciosa, the New Zealand kiwifruit. The 1.2 kb DNA fragment was acquired from the total RNAs of Chinese wild kiwifruit via reverse transcription polymerase chain reaction (RT-PCR), and its DNA sequence was analyzed. Its sequence was determined to share 98.4% homology with the actinidin gene of A. deliciosa. In order to verity the actinidin gene isolated from the Chinese wild kiwifruit in Escherichia coli, the mature gene was amplified via PCR and expressed in E. coli under the control of the T7lac promoter. The actinidin was expressed in E. coli as inclusion bodies, which were solubilized with urea and refolded. The protease activity of the refolded protein was approximately twice as high as that of E. coli BL2l (DE3).
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Response surface methodology was employed to optimize extraction conditions for finding the maximal functional properties of Pleurotus eryngii. Based on central composite design, the study plan was established with variations of microwave power (30-150 W), ethanol concentration (0-99.9%), and extraction time (1-9 min). Regression analysis was applied to obtain a mathematical model. A maximal yield of 47.86% was obtained when the microwave power, ethanol concentration, and extraction time were set at 122.7 W, 42.14%, and 8.3 min, respectively. A maximized electron donating ability of 93.32% was found under the following conditions: a microwave power of 144.19 W, an ethanol concentration of 49.52%, and an extraction time of 6.7 min. When the microwave power, ethanol concentration, and extraction time were set at 125.43 W, 40.54%, and 8.1 min, respectively, the maximum nitrite-scavenging ability was 80.47%. The optimum ranges of the extraction conditions, superimposed by the response surface methodology, could predicate a microwave power of 110-150 W, ethanol concentration of 0-45%, and extraction time of 7-9 min.
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Kim, Yu-Wang;Kim, Ji-Oh;Baek, Dong-Chul;Song, Kwan-Jeong;Kim, In-Jung;Lee, Sam-Pin;Kim, Chan-Shick 306
Setoka (Tangor Norin No.8) is a superior tangor cultivar cultivated in a greenhouse on Jeju Island, and its${\beta}-cryptoxanthi$ n content was determined during the ripening season (September 2005 to March 2006). The${\beta}-cryptoxanthi$ n content of the peel of Setoka fruits was higher than that of the flesh. Also, the${\beta}-cryptoxanthi$ n content in both peel and flesh gradually increased through the ripening season from the beginning of pigmentation, and then decreased slightly late in the ripening season. The${\beta}-cryptoxanthi$ n content in the peel of Setoka fruits varied throughout the season with values of 0.02 (Sep), 0.67 (Nov.), 2.27 (Dec.), 2.88 (Jan.), 2.27 (Feb.), and 2.13 mg% (Mar.). The${\beta}-cryptoxanthi$ n content in Setoka fruit flesh increased throughout the ripening season with values of 0.05 (Sep.), 0.22 (Nov.), 0.57 (Dec.), 0.80 (Jan.), and 0.91 mg% (Feb). -
Cui, Zhi-Gang;Kim, Bo-Youn;Kang, Shin-Hae;Lee, Young-Jae;Lee, Dae-Ho;Lee, Young-Ki;Park, Deok-Bae 311
Citrus sunki Hort. ex Tanaka is one of several Jeju-native citrus fruits. A number of biological properties for citrus fruits have been suggested, however little is known about those of C. sunki. The present study tested whether fermented product of C. sunki peel (FSP) might affect the activities of enzymes regulating ethanol metabolism. Effects on body weight gain as well as on fatty liver formation were also investigated. The activities of alcohol dehydrogenase and aldehyde dehydrogenase were stimulated remarkably by FSP. Excessive ethanol-induced cytotoxicity was also prevented by FSP in HepG2 cells. FSP decreased the weight gain and fatty liver formation induced by a high-fat diet in mice. From these results, FSP might be a potent source of neutraceuticals useful for preventing ethanol-induced health problems. -
Biofilm formation on various surfaces is a well-known phenomenon and it has caused pollution problems, health and safety hazards, and substantial economic loss in many areas including the food industry. In the present study, Gamma irradiation at a dose of 2.0 kGy reduced the bacterial counts of Staphylococcus aureus and Pseudomonas aeruginosa suspensions by 6.7 and >6.5 log CFU/mL, respectively, and 30 ppm of sodium hypochlorite effectively reduced the counts of both bacterial suspensions to below the limit of detection (
$<2\;log\;CFU/cm^2$ ). However, in bacterial biofilms attached to stainless steel, gamma irradiation at a dose of 10.0 kGy reduced the counts of S. aureus attached fur 1 hr and overnight by${\geq}5.1\;and\;5.0\;log\;CFU/cm^2$ , respectively. Gamma irradiation at a dose of 1.0 kGy reduced the counts of P. aeruginosa counts to below the limit of detection ($<2\;log\;CFU/cm^2$ ). On the contrary, S. aureus and P. aeruginosa cells attached to stainless steel chips were difficult to eliminate using sodium hypochlorite. Four hundred ppm of sodium hypochlorite reduced the counts of S. aureus and P. aeruginosa attached for 1 hr by 2.5 and$3.3\;log\;CFU/cm^2$ , respectively. -
The bacterial diversity in Korean soybean-fermented foods was investigated using a PCR-based approach. 16S rRNA sequences were amplified and cloned from two different soybean-fermented foods such as doenjang (soybean paste), and ganjang (soybean sauce). Staphylococcus equorum (60.6%), Tetragenococcus halophila (21.2%), Leuconostoc mesenteroides (9.1%), Lactobacillus sakei (6.1%), and Bacillus subtilis (3.0%) were detected among clones isolated from soybean paste samples and Halanaerobium sp. (37.5%), Halanaerobium fermentans (37.5%), T. halophila (12.5%), Staphylococcus sp. (6.3%), S. equorum (3.1%), and B. subtilis (3.1%) were detected among clones isolated from soybean sauce. Our approach revealed different bacterial distributions and diversity from those previously obtained using culture-dependent methods.
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Mesophilic aerobic bacterial counts were measured and compared for seasoned soybean sprouts produced in different seasons and sold in different market types. Very significant differences in microbial counts were found among seasoned soybean sprouts produced in different seasons (winter, spring, and summer) and among different market types (a traditional market, discount store, and department store). However, there was no significant difference among the stores within each market group. The interactions were significant at a 1% significance level. The variance of summer counts was much lower than spring and winter counts. Discount and department stores showed a higher variation in microbial counts than traditional markets. The microbial counts differed substantially from season to season in the same market group (summer > spring > winter) except between spring and summer in traditional markets. The microbial loads in the winter and spring seasons of seasoned soybean sprouts in traditional markets were clearly higher than in other market groups, while discount stores had the highest microbial count in summer.