안지오텐신(ACE) 저해제는 항고혈 효과를 갖고 있으므로 오랫동안 고혈압의 예방이나 치료에 이용되어 왔다. 본 연구는 재조합 대장균으로부터 새로운 ACE 저해제를 생산하고 정제하며 나아가 이들이 구조-기능 관P를 규명하기 위해 수행되었다. Saccharomyces cerevisiae의 ACE 저해 펩타이드 유전자를 함유하고 있는 재조합 pGEX-4T-3을 대장균 BL21(DE3)로 형질전환 시켰다. 재조합 pGEX-4T-3을 갖고 있는 대장균 BL21(DE3)로부터 생산된 Glutathione-s 전이효소(GST) 융합 단백질을 얻어서 그중 ACE저해 펩타이드를 Sephadex G-25 컬럼 크로마토그래피로 정제하였다. 정제된 ACE 저해 펩타이드는 타이로신-아스파틱엑시드-그리신-글리신-발린-페닐알라린-아르기닌-발린-타이로신-트레오닌의 서열을 가진 새로운 decapeptide이었고 ACE에 대하여 경쟁적으로 저해하였다.

내열성의 cyclomaltodextrin glucanotransferase (CGTase)를 이용하여 열에 안정한 싸이클린 텍스트린 (CD)을 생산하기 위하여 매우 높은 CGTase 활성을 보이는 고열성이며 호알카리성인 B-13세균을 분리하여 형태적, 생리학적 특성과 16S 리보솜 RNA 서열분석 등을 통하여 Bacillus cereus B-13으로 동정하였다. Bacillus cereus B-13 2% 가용성 전분, 1% 효모 추출물, 1% $Na_2CO_3$ 등을 함유하는 SYC 배지 (pH 8.5)에 접종하여 $50^{\circ}C$에서 24시간 배양하였을 때 최고의 CGTase 활성(130 U/ml)을 보였다. 또한 부분 정제된 CGTase의 작용 최적 온도와 pH는 각각 $60^{\circ}C$, pH 8.5-9.0 이었고 $80^{\circ}C$이하와 pH5.0-10.0에서 안정하였다. 1% 가용성 전분을 부분 정제한 CGTase와 작용시켰을 때 49%의 CD 수율을 보였다.

6면 프리즘 렌즈가 장착된 렌즈메터에서 상점의 위치로부터 시험렌즈의 편심의 위치를 알아 낼 수 있는 알고리즘을 개발하였다. 6개의 프리즘에 의해 형성되는 6개의 상점은 시험렌즈의 편심의 극좌표에 의해 변하게 되며, 비틀린 광선 추적으로 계산할 수 있다. 광학 설계 프로그램인 Code V를 사용하여 렌즈메터 광학계를 분석하고, 시험렌즈의 위치에 따른 상점의 위치 변화를 함수로 나타내서 역으로 상점의 위치로부터 시험렌즈의 편심의 위치를 알아낼 수 있는 방법을 개발하였다.

Many researchers consider the totality of Babylonian mathematics was profoundly elementary, but some of their mathematical knowledge achieved a novel comparable to the Greeks. The aim of this article is to provide a brief overview of the environmental and social background which made mathematical development. Historically, mathematics is always a product of society. So it is valuable to study historical background which have produced mathematics.

행렬의 고유치를 계산하는 방법을 가르칠 때 역거듭제곱방법을 소개한다. 이 방법의 알고리즘을 이론적인 면에서 이해하기 쉬운 알고리즘과 계산적인 면에서 효과적인 알고리즘을 비교한다.

This proposal is to present a campus landscape plan for PaiChai University. The site is located in 439-6 Doma-dong, Seo-gu, Taejon City and has an area of $208,499m^2$. The landscape plan covers the following: site inventory and analysis; integration of design program and site analysis; evaluation of design concept; schematic design including circulation form and spatial form; and design development including subarea design. In the design process, the author focused on the integration and interpretation of site conditions as related to the university buildings. He also collectively analyzed and considered the physical development of the outdoor spaces of Paichai University. The following are some of the major areas in the design of the outdoor spaces of Paichai University: 1) a main entrance space as a symbolic square, 2) Paichai central street, 3) a campus core as a greenish square, and 4) a 'Jajuro' plaza as Paichai 'Han' square. The outdoor spaces of Paichai university are created as a new type of campus realm with function, campus quality, original identity, symbolic meaning and images and beauty.

The study was designed to learn about features of landscape trees and shrubs on an exclusive area-specific outer space planting plan in Noeun Apartment Complex in Daejeon Metropolitan City. the exclusive areas were no more than $60m^2$ and $85m^2$ and the landscape areas ranged from 15% and 30%, respectively according to exclusive area-to-plottage ratios. The study showed that trees were likely to be more densely planted in exclusive areas not exceeding $60m^2$ than in exclusive areas over $85m^2$. It also found that the greater the exclusive areas were, the larger the number of species became. Regardless of the size of an exclusive use, however, the species number of evergreen trees and shrubs remained small, while there was no remarkable difference in vegetation due largely to a massive influx of special trees or shrubs such as Eastern white pines (Pinus strobus) and Korean azaleas (Rhododendron yedoensis var. poukhanense). The introduction of trees with the height over four meters and the diameter of breast height (DBH) exceeding 15 meters was strictly limited to Japanese red pins (Pinus densiflora) and Japanese zelkovas (Zelkova serrata). In conclusion, it is necessary for us to introduce fresh species of trees and especially develop evergreen trees and shrubs to make the environment of the apartment complex more comfortable and make a vegetation experience feasible.

This study aims to evaluate the cultural street and to find ways to revitalize a culture street. To achieve this, the study relied on empirical study method and the descriptive method; additionally, present condition survey was performed with a user interview. As a result, the item of 'night visit' shows the highest mean and the item of 'convenience of walking' follows. It means that this street has such a good condition in the convenience and accessibility of physical environment that it gives pedestrians pleasure like night activity and satisfying their curiosity. 5 lowest items of inquiry, however, are all about regional history and origin. It means that this cultural street did not reflect the history and embody the original form of the street. Also, this study proposes 4 type of street; a street with contents, a street with sequence, a street for pedestrians, and a street with identity.

This study was conducted under the theory of hermitage ever built from buddhist belief. Directing to hermitages within temples of Korea Jogye Order, we focused on the layout correspondence relationship among them. and 161 hermitages were the first object of the investigation. For the accomplishment of the research purpose, the general epitome and spatial specification through various literature studies, on-the-spot survey and personal communications with a lot of priests were made. As one of the basic surveys, the foundation year were thoroughly investigated, followed by the form of building, main buddha and hermitage name. The results obtained were summarized as follows : Forty five hermitages which are equivalent to twenty eight percent of total hermitages within the parish-level temples of Korea Jogye Order, were built in shilla Dynasty and twenty hermitages that are 12.4 percent of the total were constructed in Koryo Dynasty and thirty seven percent of the total were founded during both of dynasties. This fact makes us to consider that many of hermitages in those days were constructed with the simultaneity of principal temple foundation which means hermitage had responded the spirit of the times. Every hermitage has its main Buddha and this thing happened that each hermitage enshrines its main Buddha according to the thought which was developed with the Buddhism expansion and sutra that is in accordance with their own intention. .Basically a capital temple has differed in its central sanctum in accordance with resourceful sutra, and, at the same time, twenty seven in avatamsk sectenshrine for Sakyamuni as their sanction and eight for the Merciful Goddess and 42.7 percent for Chajang's hermitages. The name of hermitage not only represents its symbol but comprises the characteristic meaning of ascetic practice. 31.7 percent of the hermitages studies was found to have the names of relating to 'ascetic practice' or 'thought' followed by the 'nature' or 'environment' to 21.7 percent.

The equilibrium geometry, adiabatic electron affinity, harmonic vibrational frequencies, and infrared intensities of 1,4,6,9-Tetra chlorinated dibenzo-$\rho$-dioxin anion have been studied using B3LYP method. The local minimum conformation is found to be twisted form with $D_2$ symmetry. The predicted adiabatic electron affinity is 0.54 eV. The predicted inversion barrier between twisted and planar conformers was 0.9 kcal/mol, thus we expect that experimentally observed structure seems to be planar. the characteristic IR band of 1,4,6,9-TCDD anion appears at 1510 $cm^4$, which is a benzene ring skeleton vibration mode related with CaCb and C2C3 bond. this characteristic band could be sued for identication of 1,4,6,9-TCDD anion.

대청호에서 세균군집의 생태학적 역할을 조사하기 위하여 대청호 본 댐과 대전 취수탑의 중간 지점인 대청호의 만입부 지점에서 1998년 9월~1999년 8월까지 DAPI 염색법으로 수심에 따른 총 세균수를 조사하였다. 조사지점의 수표면, 수심 5m, 10m, 15m, 및 25m에서 각각 채수하여 총 세균수를 측정하고 이화학적 수질요인과 총 엽록소 $\alpha$의 농도를 측정하였다. 총 세균수는 표층수에서 $1.6-1.7.0x10^6$ cells/ml, 수심 5m에서는 $2.3-11.0x10^6$ cells/ml, 수심 10m에서는 $1.2-1.4.0x10^6$ cells/ml, 수심 15m에서는$1.4-15.0x10^6$ cells/ml, 그리고 수심 25m에서는 $1.4-1.3.0x10^6$ cells/ml이었으며, 본 조사에서는 4월경부터 수온 증가에 따라 표층수의 총세균수는 증가하기 시작하여 7월경에 최대에 도달하였고, 이때 수심이 깊어질수록 총세균수는 감소하는 경향을 보였다. 각 수심에서 총세균수와 총엽록소 $\alpha$ 량 및 이화학적 수질요인과의 관계를 논의하였다.