Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Revealing the global mechanism related to carnosine synthesis in the pectoralis major of slow-growing Korat chickens using a proteomic approach

  • Panpradub Sinpru (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Chanadda Suwanvichanee (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Rujjira Bunnom (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Satoshi Kubota (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Jirawat Yongsawatdigul (School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Wittawat Molee (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology) ;
  • Kanjana Thumanu (Synchrotron Light Research Institute (Public Organization)) ;
  • Amonrat Molee (School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology)
  • Received : 2024.02.27
  • Accepted : 2024.05.15
  • Published : 2024.10.01
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Abstract

Objective: This study aimed to find global mechanisms related to carnosine synthesis in slow-growing Korat chickens (KRC) using a proteomic approach. Methods: M. pectoralis major samples were collected from 10-week-old female KRC including low-carnosine (LC, 2,756.6±82.88 ㎍/g; n = 5) and high-carnosine (HC, 4,212.5 ±82.88 ㎍/g; n = 5). Results: We identified 152 common proteins, and 8 of these proteins showed differential expression between the LC and HC groups (p<0.05). Heat shock 70 kDa protein 8, Heat shock 70 kDa protein 2, protein disulfide isomerase family A, member 6, and endoplasmic reticulum resident protein 29 were significantly involved in protein processing in the endoplasmic reticulum pathway (false discovery rate<0.05), suggesting that the pathway is related to differential carnosine concentration in the M. pectoralis major of KRC. A high concentration of carnosine in the meat is mainly involved in low abundances of Titin isoform Ch12 and Connectin and high abundances of M-protein to maintain homeostasis during muscle contraction. These consequences improve meat characteristics, which were confirmed by the principal component analysis. Conclusion: Carnosine synthesis may occur when muscle cells need to recover homeostasis after being interfered with carnosine synthesis precursors, leading to improved muscle function. To the best of our knowledge, this is the first study to describe in detail the global molecular mechanisms in divergent carnosine contents in meat based on the proteomic approach.

Keywords

Acknowledgement

This work was supported by Suranaree University of Technology (SUT), Thailand Science Research and Innovation (TSRI), and National Science, Research and Innovation Fund (NSRF) (Project code 90464, Full-time 61/37/2564 and NRIIS number 160352); SUT and the NSRF via the Program Management Unit for Human Resources & Institutional Development, Research and Innovation (PMU-B) (Grant number B13F660067); and Suranaree University of Technology for the financial support through the Potential Graduate Students Scholarship. We also thank the Center of Excellence on Technology and Innovation for Korat Chicken Business Development (grant number CoE3-303-62-60-02), the National Research Council of Thailand (NRCT), and Beamline 4.1 Infrared Spectroscopy and Imaging (Synchrotron Light Research Institute [Public Organization], Nakhonratchasima, Thailand) for their kind support in terms of experiment animal, sample analysis tools, and statistical data. We also appreciate the support from PS Nutrition Company Limited and the Proteomics Unit core facility, University of Helsinki, Finland for experimental diets and technical support on label-free proteomic analysis, respectively.

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