대한한의학방제학회지 (Herbal Formula Science)

  • 제32권1호
  • /
  • Pages.29-37
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  • 2024
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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생쥐 대장 카할세포에서 가미소요산, 반하사심탕 및 보중익기탕의 효과에 관한 비교연구

Effects of Gamisoyo-san, Banhasasim-tang and Bojungikki-tang in Colonic Interstitial cells of Cajal in mice

  • 최나리 (부산대학교 한의학전문대학원 양생기능의학교실) ;
  • 최우균 (부산대학교 한의학전문대학원 양생기능의학교실) ;
  • 김병주 (부산대학교 한의학전문대학원 양생기능의학교실)
  • Na Ri Choi (Department of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University) ;
  • Woo-Gyun Choi (Department of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University) ;
  • Byung Joo Kim (Department of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
  • 투고 : 2024.01.16
  • 심사 : 2024.02.05
  • 발행 : 2024.02.28
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초록

Objectives : The purpose of this study was to examine the effects of insurance herbal medicines on colonic interstitial Cells of Cajal (ICC) in mice. Methods : Among the insurance herbal medicines, we chose Gamisoyo-san (GSS), Banhasasim-tang (BHSST) and Bojungikki-tang (BGIKT). We made the ICC culture in large intestine in mice and used the electrophysiological method to record pacemaker potentials. Also we used MTT assay to check cell viability and examined the ICC protein expression by western blot. Results : 1. GSS (1-10 mg/ml) induced the pacemaker potential depolarization and decreased frequency with concentration-dependent manners in colonic ICC. EC50 is 2.99 mg/ml. BHSST (1-10 mg/ml) induced the pacemaker potential depolarization and decreased frequency with concentration-dependent manners in colonic ICC. EC50 is 2.76 mg/ml. BGIKT (1-10 mg/ml) induced the pacemaker potential depolarization and decreased frequency with concentration-dependent manners in colonic ICC. EC50 is 4.49 mg/ml. 2. GSS, BHSST and BGIKT had no effects on cell viability in colonic ICC. 3. GSS and BGIKT increased the Anoctamin-1 (ANO1) protein expression and BHSST increased the transient receptor potential melastatin-subfamily member 7 (TRPM7) protein expression in colonic ICC. Conclusions : These results suggest that GSS, BHSST, and BGIKT have shown the potential to regulate gastrointestinal (GI) motility by regulating colonic ICC and may show the potential to treat colon-derived GI diseases such as irritable bowel syndrome (IBS).

키워드

과제정보

본 연구는 보건복지부의 재원으로 한국보건산업진흥원의 보건의료기술연구개발사업 지원에 의하여 이루어진 것임 (과제번호 HF22C0063).

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