Mycobiology

The Korean Society of Mycology (한국균학회)
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Promotion of Tricholoma matsutake mycelium growth by Penicillium citreonigrum

  • Doo-Ho Choi (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jae-Gu Han (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kang-Hyo Lee (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • An Gi-Hong (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA)
  • Received : 2023.06.21
  • Accepted : 2023.09.06
  • Published : 2023.10.31
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Abstract

Tricholoma matsutake has been the most valuable ectomycorrhizal fungi in Asia because of its unique flavor and taste. However, due to the difficulty of artificial cultivation, the cultivation of T. matsutake has relied on natural growth in forests. To cultivate the T. matsutake artificially, microorganisms in fairy rings were introduced. In this study, we isolated 30 fungal species of microfungi from the soil of fairy rings. Among them, one single fungal strain showed a promoting effect on the growth of T. matsutake. The growth effect was confirmed by measuring the growth area of T. matsutake and enzyme activities including a-amylase, cellulase, and b-glucosidase. In comparison with control, microfungal metabolite increased the growth area of T. matsutake by 213% and the enzyme activity of T. matsutake by 110-200%. The isolated fungal strain was identified as Penicillium citreonigrum by BLAST on the NCBI database. The Discovery of this microfungal strain is expected to contribute to artificial cultivation of T. matsutake.

Keywords

Acknowledgement

This study was conducted in the postdoctoral research program support project as part of the results conducted by the Rural Development Administration (PJ014766022023).

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