Mycobiology

The Korean Society of Mycology (한국균학회)
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Transcriptome Analysis Reveals the Putative Polyketide Synthase Gene Involved in Hispidin Biosynthesis in Sanghuangporus sanghuang

  • Jiansheng Wei (Haba Snow Mountain Provincial Nature Reserve Management and Protection Bureau) ;
  • Liangyan Liu (College of Agronomy and Biotechnology, Yunnan Agriculture University) ;
  • Xiaolong Yuan (Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry & Grassland) ;
  • Dong Wang (Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry & Grassland) ;
  • Xinyue Wang (Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry & Grassland) ;
  • Wei Bi (Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry & Grassland) ;
  • Yan Yang (Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences) ;
  • Yi Wang (Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry & Grassland)
  • Received : 2023.06.06
  • Accepted : 2023.08.30
  • Published : 2023.10.31
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Abstract

Hispidin is an important styrylpyrone produced by Sanghuangporus sanghuang. To analyze hispidin biosynthesis in S. sanghuang, the transcriptomes of hispidin-producing and non-producing S. sanghuang were determined by Illumina sequencing. Five PKSs were identified using genome annotation. Comparative analysis with the reference transcriptome showed that two PKSs (ShPKS3 and ShPKS4) had low expression levels in four types of media. The gene expression pattern of only ShPKS1 was consistent with the yield variation of hispidin. The combined analyses of gene expression with qPCR and hispidin detection by liquid chromatography-mass spectrometry coupled with ion-trap and time-of-flight technologies (LCMS-IT-TOF) showed that ShPKS1 was involved in hispidin biosynthesis in S. sanghuang. ShPKS1 is a partially reducing PKS gene with extra AMP and ACP domains before the KS domain. The domain architecture of ShPKS1 was AMP-ACP-KS-AT-DH-KR-ACP-ACP. Phylogenetic analysis shows that ShPKS1 and other PKS genes from Hymenochaetaceae form a unique monophyletic clade closely related to the clade containing Agaricales hispidin synthase. Taken together, our data indicate that ShPKS1 is a novel PKS of S. sanghuang involved in hispidin biosynthesis.

Keywords

Acknowledgement

The research was funded by General Project of Basic Research Program in Yunnan Province (project no. 202101AT070218), the National Natural Science Foundation of China (Project No. 31860177), and the Reserve Talents for Young and Middle-aged Academic and Technical Leaders of the Yunnan Province (Project No. 202205AC160044).

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