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Assessment of the macroalgal diversity of Kuwait by using the Germling Emergence Method

  • Amal H. Hajiya Hasan (School of Biological Sciences, University of Aberdeen) ;
  • Dhia A. Al-Bader (Department of Biological Sciences, Faculty of Science, Kuwait University) ;
  • Steve Woodward (School of Biological Sciences, University of Aberdeen) ;
  • Csongor Z. Antony (School of Biological Sciences, University of Aberdeen) ;
  • Jared Kok Ong (School of Biological Sciences, University of Aberdeen) ;
  • Akira F. Peters (School of Biological Sciences, University of Aberdeen) ;
  • Frithjof C. Kupper (School of Biological Sciences, University of Aberdeen)
  • Received : 2022.08.06
  • Accepted : 2023.04.30
  • Published : 2023.06.21
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Abstract

Cryptic stages of diverse macroalgae present in natural substrata, "the bank of microscopic forms", were isolated into clonal cultures and identified based on both morphological characteristics and DNA barcoding. Approximately 120 clonal isolates from 308 natural substratum samples were collected from the entire coastline of Kuwait. Amongst these isolates, 77 (64%) were identified through DNA barcoding using the nuclear ribosomal small subunit, RuBisCO spacer (ITS2, tufa, rbcL, psaA, and psbA) and sequencing. Twenty-six isolates (34%) were identified in the division Chlorophyta, 18 (23%) as Phaeophyceae, and 33 (43%) as Rhodophyta. For all DNA sequences in this study, species-level cut off applied was ≥98% homology which depend entirely on the markers used. Three putative new records of Chlorophyta new for the Arabian Gulf were made: Cladophora laetevirens (Dillwyn) Kützing, Ulva torta (Mertens) Trevisan and Ulvella leptochaete (Huber) R. Nielsen, C. J. O'Kelly & B. Wysor in Nielsen, while Cladophora gracilis Kützing and Ulva ohnoi M. Hiraoka & S. Shimada are new records for Kuwait. For Phaeophyceae, Ectocarpus subulatus Kützing and Elachista stellaris Areschoug were new records for the Gulf and Kuwait. In the Rhodophyta, Acrochaetium secundatum (Lyngbye) Nägeli in Nägeli & Cramer, Ceramium affine Setchell & N. L. Gardner, Gelidium pusillum var. pakistanicum Afaq-Husain & Shameel and Dasya caraibica Børgesen are new records for the Gulf and Kuwait, while the red alga Stylonema alsidii (Zanardini) K. Drew is a new record for Kuwait. Several isolates identified corresponded to genera not previously reported in Kuwait and / or the Arabian Gulf, such as Porphyrostromium Trevisan, a new genus from the Bangiales, and two unidentified species for the Planophilaceae Škaloud & Leliaert. The isolates cultivated from substrata enhance understanding of the marine macroalgal diversity in the region and confirmed that the Germling Emergence Method is suitable for determining the actual diversity of a given study area through isolation from cryptic life-history phases.

Keywords

Acknowledgement

The present work formed part of the first author's PhD thesis 'Macroalgal biodiversity of Kuwait, with special emphasis on the vicinity of desalination plants'. We acknowledge Dr. Hedda Weitz (University of Aberdeen) for providing help in the laboratory and from Ioanna Kosma (University of the Aegean) and Andreas Henkel (Kuwait University) for diving and logistics support during the expedition to Kuwait. We acknowledge the funding received to support this work from the Marine Alliance for Science and Technology (grant reference HR09011) to FCK and Kuwait Foundation for the Advancement of Science (KFAS; grant number PR17125L18) to DA. To Mr. Yusuf Buhadi from the department of Marine Sciences at Kuwait University for his help in the field work and to Mrs. Nisha V. S. Vadakkhancheril for photography.

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