Current Optics and Photonics

Optical Society of Korea (한국광학회)
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Autofluorescence Loss in Photobleaching for Human Dentin ex vivo

  • Lee, Seunghwan Goldmund (Department of Opto-mechatronics and Cogno-mechatronics Engineering, Pusan National University) ;
  • Kim, Minwoo (Department of Opto-mechatronics and Cogno-mechatronics Engineering, Pusan National University) ;
  • Jeong, Sunghee (Department of Oral Medicine, School of Dentistry, Pusan National University) ;
  • Hwang, Jaejoon (Department of Oral and Maxillofacial Radiology, School of Dentistry, Pusan National University, Dental and Life Science Institute) ;
  • Kim, Jisu (Department of Opto-mechatronics and Cogno-mechatronics Engineering, Pusan National University) ;
  • Gourrier, Aurelien (University Grenoble Alpes, Laboratoire Interdisciplinaire de Physique) ;
  • Vial, Jean Claude (University Grenoble Alpes, Laboratoire Interdisciplinaire de Physique) ;
  • Kyhm, Kwangseuk (Department of Opto-mechatronics and Cogno-mechatronics Engineering, Pusan National University)
  • Received : 2021.11.16
  • Accepted : 2022.01.10
  • Published : 2022.02.25
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Abstract

Two-photon fluorescence microscopy was performed on the enamel-dentin junction area of a human tooth using a femtosecond pulsed laser. We obtained a clear image contrast between the bright dentin and dark tubules with the autofluorescence generated from the endogenous fluorophores in dentin. The autofluorescence shows a broad spectrum due to complex cross links between dentinal collagens, which extend from blue to orange wavelengths (470-590 nm), but a gradual autofluorescence loss in photobleaching was observed for a long-term exposure under strong excitation. For increasing excitation power, we found that two-step decay becomes significant in the spectrally integrated autofluorescence.

Keywords

Acknowledgement

This research was supported by PNU-RENovation (2020-2021).

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