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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Mycoplasma exploits mammalian tunneling nanotubes for cell-to-cell dissemination

  • Kim, Bong-Woo (Tunneling Nanotube Research Center, Korea University) ;
  • Lee, Jae-Seon (Department of Molecular Medicine, College of Medicine, Inha University) ;
  • Ko, Young-Gyu (Tunneling Nanotube Research Center, Korea University)
  • Received : 2018.10.15
  • Accepted : 2018.11.04
  • Published : 2019.08.31
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Abstract

Using tunneling nanotubes (TNTs), various pathological molecules and viruses disseminate to adjacent cells intercellularly. Here, we show that the intracellular invasion of Mycoplasma hyorhinis induces the formation of actin- and tubulin-based TNTs in various mammalian cell lines. M. hyorhinis was found in TNTs generated by M. hyorhinis infection in NIH3T3 cells. Because mycoplasma-free recipient cells received mycoplasmas from M. hyorhinis-infected donor cells in a mixed co-culture system and not a spatially separated co-culture system, direct cell-to-cell contact via TNTs was necessary for the intracellular dissemination of M. hyorhinis. The activity of Rac1, which is a small GTP binding protein, was increased by the intracellular invasion of M. hyorhinis, and its pharmacological and genetic inhibition prevented M. hyorhinis infection-induced TNT generation in NIH3T3 cells. The pharmacological and genetic inhibition of Rac1 also reduced the cell-to-cell dissemination of M. hyorhinis. Based on these data, we conclude that intracellular invasion of M. hyorhinis induces the formation of TNTs, which are used for the cell-to-cell dissemination of M. hyorhinis.

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References

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