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Analysis of Selected Water-Soluble Vitamin B1, B2, B3, and B12 Contents in Namul (Wild Greens) Consumed in Korea

국내에서 섭취되는 나물류에 함유된 일부 수용성 비타민의 함량 분석

  • Yoon, Jimin (Dept. of Food Science and Biotechnology, Kyungsung University) ;
  • Chung, Haejung (Dept. of Korean Culinary Arts, Jeonju University) ;
  • Kim, Younghwa (School of Food Biotechnology and Nutrition, Kyungsung University)
  • 윤지민 (경성대학교 식품생명공학과 대학원) ;
  • 정혜정 (전주대학교 한식조리학과) ;
  • 김영화 (경성대학교 식품응용공학부)
  • Received : 2019.01.25
  • Accepted : 2019.02.09
  • Published : 2019.02.28

Abstract

In this study, the contents of water-soluble vitamins $B_1$ (thiamin), $B_2$ (riboflavin), $B_3$ (niacin), and $B_{12}$ (cyanocobalamin) in namuls (wild greens), such as salads and side dishes, consumed in Korea were quantified by high-performance liquid chromatography (HPLC) with UV and fluorescence detection. All samples were prepared with recipes used in Korea. All analyses were performed under the quality control of vitamin $B_1$, $B_2$, $B_3$, and $B_{12}$. The vitamin $B_1$, $B_2$, and $B_3$ contents in namuls consumed in Korea were analyzed. The highest content of vitamin $B_1$ was $3.018{\pm}0.016mg/100g$ in Putgochudoenjang-muchim. The highest contents of vitamin $B_2$, $B_3$, and $B_{12}$ were $0.279{\pm}0.003mg/100g$ in Gul-muchim, $12.241{\pm}0.040mg/100g$ in Chamchi-salad, and $8.133{\pm}0.371{\mu}g/100g$ in Pijogae-muchim, respectively. These results showed that animal-based ingredients in salads provided for good intake of vitamin $B_{12}$. These results can be used as basic data for food composition tables and improvement of the national health of Koreans.

Keywords

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Fig. 1. Quality control charts of vitamin B1 (A), B2 (B), B3 (C), and B12 (D) analysis.

Table 1. HPLC operating condition for vitamin B1 and B3 analysis

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Table 2. HPLC operating condition for vitamin B2 analysis

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Table 3. HPLC operating condition for vitamin B12 analysis

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Table 4. Comparison of the LOD and LOQ value of vitamin B1, B2, B3 and B12

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Table 5. Recovery and RSD values of vitamin B1, B2, B3 and B12 contents for SRM (standard reference material)

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Table 6. Vitamin B1, B2, B3 and B12 contents of namuls consumed in Korea

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