Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A MALDI-MS-based Glucan Hydrolase Assay Method for Whole-cell Biocatalysis

  • Ahn, Da-Hee (Department of Chemical Engineering, Soongsil University) ;
  • Park, Han-Gyu (Department of Chemical Engineering, Soongsil University) ;
  • Song, Won-Suk (School of Chemical and Biological Engineering, Seoul National University) ;
  • Kim, Seong-Min (Department of Chemical Engineering, Soongsil University) ;
  • Jo, Sung-Hyun (Department of Chemical Engineering, Soongsil University) ;
  • Yang, Yung-Hun (Department of Biological Engineering, Konkuk University) ;
  • Kim, Yun-Gon (Department of Chemical Engineering, Soongsil University)
  • Received : 2018.10.16
  • Accepted : 2018.10.31
  • Published : 2019.03.28
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Abstract

Screening microorganisms that can produce glucan hydrolases for industrial, environmental, and biomedical applications is important. Herein, we describe a novel approach to perform glucan hydrolase screening-based on analysis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) spectra-which involves degradation of the oligo- and polysaccharides. As a proof-of-concept study, glucan hydrolases that could break down glucans made of several glucose units were used to demonstrate the MALDI-MS-based enzyme assay. First, the enzyme activities of ${\alpha}$-amylase and cellulase on a mixture of glucan oligosaccharides were successfully discriminated, where changes of the MALDI-MS profiles directly reflected the glucan hydrolase activities. Next, we validated that this MALDI-MS-based enzyme assay could be applied to glucan polysaccharides (i.e., pullulan, lichenan, and schizophyllan). Finally, the bacterial glucan hydrolase activities were screened on 96-well plate-based platforms, using cell lysates or samples of secreted enzyme. Our results demonstrated that the MALDI-MS-based enzyme assay system would be useful for investigating bacterial glucoside hydrolases in a high-throughput manner.

Keywords

References

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