Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
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Treatment with ultra-dilutions of Arnica montana increases COX-2 expression and PGE2 secretion in mouse chondrocytes

생쥐 연골세포에 Arnica montana 처리에 따른 COX-2 발현과 PGE2 분비 비교

  • Kim, Yun Kyu (Department of Medicine, University of Ulsan College of Medicine) ;
  • Yeo, Myeong Gu (Department of Integrative Medical Sciences, Nambu University)
  • 김윤규 (울산대학교 의과대학) ;
  • 여명구 (남부대학교 통합의료학과)
  • Received : 2019.01.15
  • Accepted : 2019.02.20
  • Published : 2019.02.28
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Abstract

Objective: We studied the effects of 4x, 30x, 30c, and 200c homeopathic dilutions of A. montana on inflammation in primary cultured mouse chondrocytes. Methods: Examined expression of Coll-2 and COX-2, and secretion of PGE2. Results: Treatment with 4x, 30x, and 30c A. montana decreased mRNA expression of Coll-2 and 30x A. montana increased mRNA expression of COX-2, while treatment with 30x and 30c A. montana increased protein expression of COX-2. Treatment with the 30c A. montana increased release of PGE2. Conclusion: Treatment with A. montana induces dedifferentiation and inflammatory responses, including increased COX-2 expression and PGE2 secretion.

연구목적 : 4x, 30x, 30c, and 200c 농도의 동종약물 Arnica montana (A. montana)를 1차 배양된 생쥐 연골세포에 적용하여 염증관련 인자의 변화를 관찰하고자 하였다. 연구방법 : 본 연구는 collagen type II (Coll-2), cyclooxygenase-2 (COX-2) 발현 그리고 prostaglandin 2 (PGE2) 분비에 대해 조사하였다. 결과: 4x, 30x 그리고 30c의 A. montana를 처리하였을 때, Coll-2의 mRNA 발현이 감소하였으며, 30x A. montana의 경우 COX-2 mRNA의 발현이 증가하였다. 또한 COX-2 단백질 발현은 30x와 30c의 A. montana 처리 시 증가함을 보였다. PGE2 분비 또한 30c에서 증가함을 관찰하였다. 결론 : A. montana 처리에 따라 생쥐의 연골세포의 분화 억제를 확인하였으며, 염증관련 인자인 COX-2 및 PGE2의 발현이 증가함을 확인하였다.

Keywords

OHHGBW_2019_v10n2_331_f0001.png 이미지

Fig. 1. Treatment with homeopathic dilutions of A. montana does not affect proliferation of chondrocytes or elicit cytotoxic effects.

OHHGBW_2019_v10n2_331_f0002.png 이미지

Fig. 2. Treatment with the 30x homeopathic dilution of A. montana increases mRNA expression of COX-2 in chondrocytes.

OHHGBW_2019_v10n2_331_f0003.png 이미지

Fig. 3. Treatment with 4x, 30x, and 30c homeopathic dilutions of A. montana increases protein expression of COX-2 in chondrocytes.

OHHGBW_2019_v10n2_331_f0004.png 이미지

Fig. 4. Treatment with the 30c homeopathic dilution of A. montana increases release of PGE2 by chondrocytes.

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