Journal of Haehwa Medicine (혜화의학회지)

Research Institute of Korean Medicine (대전대학교 한의학연구소)
권호 표지

Effects of Gamishinchubogun-tang on Regeneration of PC12 Cells

가미신추보건탕(加味伸椎步建湯)이 PC12 세포의 재생에 미치는 영향

  • Gu, Ji-Hyang (Dept. of Korean Medicine Rehabilitation College of Korean Medicine, Daejeon University) ;
  • Lee, Chi-Ho (Dept. of Korean Medicine Rehabilitation College of Korean Medicine, Daejeon University) ;
  • Lee, Eun-Jung (Dept. of Korean Medicine Rehabilitation College of Korean Medicine, Daejeon University)
  • Received : 2016.08.08
  • Accepted : 2016.08.15
  • Published : 2016.08.20
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Abstract

Objectives : This study was designed to investigate the effect of Gamishinchubogun-tang (JiaweiShenzhuibujian-tang; GSB) on regeneration of PC12 cells. Methods : PC12 cells have been used extensively as a model for studying the cellular and molecular effects of neuronal cells. In order to check the effect of GSB on the regeneration of PC12 cells, the morphological change of PC12 cells were observed comparatively in GSB group and control group. Results : The significant changes in neurite length of PC12 cells have been observed on GSB group. In proportion to the concentration of GSB it was observed an increase in neurite outgrowth. Conclusions : This study confirmed that GSB made a significant influence on regeneration of PC12 cells.

Keywords

References

  1. The Korean Academy of Oriental Rehabilitation Medicine. Oriental Rehabilitaion Medicine. Seoul, Koon ja, p.56, 2001.
  2. Song BK, Study on the Conservative Treatment of Herniated Lumbar Intervertebral Disc. Journal of Korean Medicine, 16(2):62-78, 1995.
  3. Lee GM, Lee KC, Hwang YJ, Collaborative study of oriental-western medicine on HIVD. J Kor Acu Mox Soc, 17: 1-10, 2000.
  4. The Korean Orthopaedic Association. Orthopedics. Seoul, Recent medical history, p.860-874, 2013.
  5. Sin MS, Roh JD, Choi SW, Lee DH, Song BY, Yook TH, Clinical Studies on Ruptured Intervertebral Lumbar Discs. The Acupuncture, 18(6): 84-92, 2001.
  6. Sun SM, Emergency Formulas Worth a Thousand in Gold. Beijing, Huaxia publishers Co, p.132, 1993.
  7. Moon HJ, Lim EM, Effects of Gagamdokhwalgisang-Tang on the Morphometric Changes of Femur and the Factors Related with Bone Metabolism in Ovariectomized Rats. The Journal of Oriental Obstetrics and Gynecology, 19(1): 47-68, 2006.
  8. Heo J, Donguibogam. Seoul, BubIn publishers Co: p.850-851, 2007.
  9. Choi CH, Kim SJ, Shin JC, Wi TS, Kim JH, Choi JB, et al., Clinical Study of Dokhwalkigisaengtanggagambang(DGG) and Gamisayuktanggagambang(GSG) for Improving Lumbago and Knee Joint Pain. The Society for Herbal, 28(2): 75-82, 2013.
  10. Lee SG, Won JK, Yeom SR, Lee SK, Song YS, Kwon YD, The Effects of Dokhwalgisaeng-tang(Duhuoqisheng-ta ng) and Jungsongouhyul Pharmacopuncture on Pain Control and Nerve Regeneration in the Crush-induced Sciatic Nerve Injury of the Rat Model. Journal of oriental rehabilitation medicine, 19(3): 15-32, 2009.
  11. Lee EJ, Choi JJ, Jang ES, Park Yc, Jung IC, Effects of Shinchubogun-tang and Common Korean Medicine Treatment on Symptom Reduction in Patients with Intervertebral Lumbar Disc Herniation : A Retrospective Observational Study. Journal of Physiology & Pathology in Korean Medicine, 30(1): 66-72, 2016. https://doi.org/10.15188/kjopp.2016.02.30.1.66
  12. Ide C, Peripheral nerve regeneration. Neuroscience Research, 25(2): 101-21, 1996. https://doi.org/10.1016/0168-0102(96)01042-5
  13. Coderre TJ, Katz J, Vaccarino AL, Melzack R, Contribution of central neuroplasticity to pathological pain: review of clinical and experimental evidence. Pain, 52(3): 259-85, 1993. https://doi.org/10.1016/0304-3959(93)90161-H
  14. Hoke A, Brushart T, Introduction to special issue: Challenges and opportunities for regeneration in the peripheral nervous system. Experimental neurology, 223(1): 1-4, 2010. https://doi.org/10.1016/j.expneurol.2009.12.001
  15. Brushart TM, Gerber J, Kessens P, Chen YG, Royall RM, Contributions of pathway and neuron to preferential motor reinnervation. The Journal of neuroscience, 18(21): 8674-8681, 1998. https://doi.org/10.1523/JNEUROSCI.18-21-08674.1998
  16. Faweett JW, Keynes RJ, Peripheral nerve regeneration. Annual review of neuroscience, 13(1): 43-60, 1990. https://doi.org/10.1146/annurev.ne.13.030190.000355
  17. Shin SR, Kim AL, Factors effect on quality of life of spinal cord injury patients. Journal of korean Academy of Nursing, 31(1): 126-38, 2001. https://doi.org/10.4040/jkan.2001.31.1.126
  18. DeLisa JA, Developing an integrative approach to spinal cord medicine. The journal of spinalcord medicine, 26(1): 27, 2003.
  19. Hwang KJ, Transplantation of humanneural stem cells-derived oligodendrocyte progenitors into spinal cord injury in adult rat. 연세대학교 대학원 석사학위 논문, 2010.
  20. Lee KH, Yoon DH, Park YG, Lee BH, Effects of glial transplantation on functional recovery following acute spinal cord injury. Journal of neurotrauma, 22(5): 575-589, 2005. https://doi.org/10.1089/neu.2005.22.575
  21. Kim HK, Lee JD, The follow-up computed tomographic view after treatment of HNP of L-spine by oriental medical method. Journal of Korean Medicine, 18(2): 33-42, 1997.
  22. Postacchini F, Results of surgery compared with conservative management for lumbar disc herniations. Spine, 21(11): 1383-1387, 1996. https://doi.org/10.1097/00007632-199606010-00023
  23. Saal JA, Saal JS, Nonoperative treatment of herniated lumbar intervertebral disc with radiculopathy: an outcome study. Spine, 14(4): 431-437, 1989. https://doi.org/10.1097/00007632-198904000-00018
  24. Kim JY, Lee SY, Lee KG, Effects of Dokwhalgisaengtang on the serum in Rats. Korean J. Oriental Medical Pathology, 12(2): 99-104, 1998.
  25. Shin BC, Song YS, Kwon YD, Effects of Dokhwalgisaeng-tang(Duhuojisheng-tan g) and Herbar Chelidoni on the Cultured Spinal Sensory Neurons Damaged by XO/HX. Journal of oriental rehabilitation medicine, 10(2): 69-79, 2000.
  26. College of Oriental Medicine, Co Textbook Compilation Committee. Herbal Medicine. Seoul, Yonglim, p.152-637, 2007.
  27. Lee KH, Kim CY, Kim KY, Nam HW, Jung YH, Koh YT, Case Report of L-spine HIVD treated with Spine Decompression. The Journal of Korean Society of Chuna Manual Medicine for Spine & Nerve, 2(1): 39-47, 2007.
  28. Han HJ, June LH, Kang SS, Lee SH, Cho IH, Hwan LJ, et al., Effect of Red Ginseng Total Saponin on Sciatic Nerve Regeneration. Journal of Ginseng Research, 27(3): 103-109, 2003. https://doi.org/10.5142/JGR.2003.27.3.103
  29. Gordon T, The role of neurotrophic factors in nerve regeneration. Neurosurgical focus, 26(2): E3, 2009.
  30. Park JS, The effects of treadmill exercise on axon regeneration in injured median nerve. The Korea Journal of Sports Science, 22(4): 983-994, 2013.