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Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

  • Rahman, Md. Siddiqur (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Chakrabartty, Amitavo (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Islam, Md. Taohidul (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Sarker, Roma Rani (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Alam, M.E. (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Uddin, Muhammad Jasim (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University) ;
  • Akther, Laila (Department of Livestock Services, Krishi Khamar Sarak) ;
  • Song, Hee-Jong (Korea Zoonoses Research Institute, Chonbuk National University)
  • Received : 2012.04.20
  • Accepted : 2012.06.13
  • Published : 2012.06.30

Abstract

Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise sero-prevalence was found 3.0% in 2~3 years age group and 2.0% in 4~8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

References

  1. Abubakar M, Javed Arshed M, Hussain M, Ehtisham-ul-Haq, Ali Q. 2010. Serological evidence of Brucella abortus prevalence in Punjab province, Pakistan-a cross-sectional study. Transbound Emerg Dis 57: 443-447. https://doi.org/10.1111/j.1865-1682.2010.01171.x
  2. Ahmed M, Islam MA, Khatun MM, Baek BK. 2011. Evaluation of four serological tests for the detection of brucellosis in goats and cattle under the field condition of Bangladesh. Asian J Biol Sci 4: 477-482. https://doi.org/10.3923/ajbs.2011.477.482
  3. Akbarmehr J, Ghiyamirad M. 2011. Serological survey of brucellosis in livestock animals in Sarab City (East Azarbayjan province), Iran. African J Microbiol Res 5: 1220- 1223. https://doi.org/10.5897/AJMR11.180
  4. Amin KMR, Rahman MB, Sarkar SK, Kabir SML, Akand MSI. 2004. Serological epidemiology of brucellosis in cattle of the Mymensingh district of Bangladesh. J Anim Vet Adv 3: 898-900.
  5. Chakraborty M, Patgiri GP, Sarma DK. 2000. Use of Rose Bengal plate test, serum agglutination test and indirect ELISA for detecting brucellosis in bovines. Indian J Comp Microbiol Immunol Infect Dis 21: 24-25.
  6. Das T, Ershaduzzaman M, Islam KK, Haque MM, Rahman MM, Islam KBMS. 2008. Surveillance of Brucella melitensis and Brucella abortus from aborted Bengal goats in Bangladesh. Res J Vet Sci 1: 28-36. https://doi.org/10.3923/rjvs.2008.28.36
  7. Ferreira AC, Cardoso R, Travassos Dias I, Mariano I, Belo A, Rolao Preto I, Manteigas A, Pina Fonseca A, Correa De Sa MI. 2003. Evaluation of a modified Rose Bengal test and an indirect enzyme linked immunosorbent assay for the diagnosis of Brucella melitensis infection in sheep. Vet Res 34: 297-305. https://doi.org/10.1051/vetres:2003005
  8. Gotuzzo E, Carrillo C, Guerra J, Llosa L. 1986. An evaluation of diagnostic methods for brucellosis-the value of bone marrow culture. J Infect Dis 153: 122-125. https://doi.org/10.1093/infdis/153.1.122
  9. Islam A, Haque M, Rahman A, Rahman MM, Rahman A, Haque F. 1983. Economic losses due to brucellosis among cattle in Bangladesh. Bangl Vet J 17: 5762.
  10. Kadir 2010. Seroprevalence of Brucellosis in cattle in some of the selected areas of Naogaon District. M.S. Thesis, Department of Medicine, Bangladesh Agricultural University, Mymensingh.
  11. Kebede T, Ejeta G, Ameni G. 2008. Seroprevalence of bovine brucellosis in smallholder farms in central Ethiopia (Wuchale-Jida district). Revue de Medecine Veterinaire 159: 3-9.
  12. Mia AS, Islam H. 1967. A preliminary study on the incidence of bovine infertility and economic loss caused by it. Pakistan Vet J 1: 12-15.
  13. Muma JB, Toft N, Oloya J, Lund A, Nielsen K, Samui K, Skjerve E. 2007. Evaluation of three serological tests for brucellosis in naturally infected cattle using latent class analysis. Vet Microbiol 125: 187-192. https://doi.org/10.1016/j.vetmic.2007.05.012
  14. Nahar A, Ahmed MU. 2009. Sero-prevalence study of brucellosis in cattle and contact human in Mymensingh district. Bangl J Vet Med 7: 269-274.
  15. OIE. 2009. Bovine brucellosis. Manual of diagnostic tests and vaccines for terrestrial animals. http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.04.03_BOVINE_BRUCELL.pdf.
  16. Orduna A, Almaraz A, Prado A, Gutierrez MP, Garcia-Pascual A, Duenas A, Cuervo M, Abad R, Hernandez B, Lorenzo B, Bratos MA, Torres AR. 2000. Evaluation of an immunocapture- agglutination test (Brucellacapt) for the seodiagnosis of human brucellosis. J Clin Microbial 38: 4000-4005.
  17. Probert WS, Schrader KN, Khuong NY, Bystrom SL, Graves MH. 2004. Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis. J Clin Microbiol 42: 1290-1293. https://doi.org/10.1128/JCM.42.3.1290-1293.2004
  18. Rahman MM, Choudhury TIMFR, Rahman A, Haque F. 1983. Seroprevalence of human and animal brucellosis in Bangladesh. Indian Vet J 60: 165.
  19. Rahman MS, Han JC, Park J, Lee JH, Eo SK, Chae JS. 2006. Prevalence of brucellosis and its association with reproductive problems in cows in Bangladesh. Vet Rec 159: 180-182. https://doi.org/10.1136/vr.159.6.180
  20. Rahman MS. 2010. Brucellosis: a great constraint for development of livestock. Bangl Dairy Poul 5: 18-19.
  21. Rahman MS, Faruk MO, Her M, Kim JY, Kang SI, Jung SC. 2011. Prevalence of brucellosis in ruminants in Bangladesh. Veterinarni Medicina 56: 379-385.
  22. Singh G, Sharma DR, Dhand NK. 2004. Seroprevalence of bovine brucellosis in Punjab. Indian Vet J 81:620-623.
  23. Yagupsky P, Baron EJ. 2005. Laboratory exposures to Brucellae and implications for bioterrorism. Emerg Infect Dis 11: 1180-1185. https://doi.org/10.3201/eid1108.041197
  24. Young EJ. 1995. An overview of human brucellosis. Clin Infect Dis 21: 283-290. https://doi.org/10.1093/clinids/21.2.283