Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Callus formation Ratio and Regeneration Efficiency of Orchardgrass Varieties Developed in Korea

국내육성 오차드그라스 품종들의 캘러스 형성율 및 식물체 재분화 효율

  • Kim, Ki-Yong (National Livestock Research Institute) ;
  • Kang, Kyung-Min (National Livestock Research Institute) ;
  • Bae, Eun-Kyung (College of Lige Sci. & Agriculture, Kyungpook Nat'l. Univ.) ;
  • Lee, In-Ae (College of Lige Sci. & Agriculture, Kyungpook Nat'l. Univ.) ;
  • Rim, Yong-Woo (National Livestock Research Institute) ;
  • Choi, Gi-Jun (National Livestock Research Institute) ;
  • Park, Geun-Je (National Livestock Research Institute) ;
  • Son, Dae-Young (PMBBRC and Dept. Molecular Biology, Gyeongsang Natl. Univ.) ;
  • Jo, Jin-Ki (College of Lige Sci. & Agriculture, Kyungpook Nat'l. Univ.)
  • Published : 2003.03.01
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Abstract

Comparisons of callus formation ratios from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency for 4 orchardgrass (Dactylis glomerata L.) varieties (Three were developed in Korea and one was imported from foreign nation) are as follows; Jangbeol 102 (67.0%) has the highest callus formation ratio in 4 weeks incubated callus after bedding the seed explants, but Potomac (68.4%) has the highest ratio in 6-week callus. Potomac (3.91cm) has the highest callus size in 4-week callus, but Jangbeol 101 (4.32cm) has the highest size in 6-week callus. Jangbeol 101 (17.7%) has the highest plant regeneration ratio in 4-week callus, but Potomac (37.4%) has the highest ratio in f-week callus. Jangbeol 102 (11.5%) has the highest plant regeneration efficiency in 4-week callus, but Potomac (25.6%) has the highest efficiency in 6-week callus.

국내육생 품종인 Hapsung 2, Jangbeol 101, Jangbeol 102와 대비품종인 Potomac의 종자로부터의 캘러스 형성율, 캘러스 크기, 식물체 재 분화율 및 재분화 효율을 4주령 및 6주령의 캘러스에 대하여 조사한 결과는 다음과 같다. 캘러스 형성율은 4주령 캘려스에서는 Jangbeol 102가 가장 높았고, 6주령 캘러스에서는 대비 품종인 Potomac아 가장 높았다. 캘러스 크기는 4주령 캘러스에서 대비품종인 Potomac (3.93cm)이 가장 컸으며, 6주령 캘러스에서는 Jangbeol 101 (4.32cm)이 가장 컸지만, 품종간의 차이는 적은 편이었다. 식물체 재분화율운은4주령 캘러스에서 10.3~17.7%의 넓은 범위의 차이를 보였는데, Jangbeol 101이 가장 높았으며 6주령 캘러스에서도 역시 11.2~37.4%로서 품종간에 큰편차를 보였으며, 특히 대비품종인 Potomac은 월등하게 재분화율이 높았다. 식물체 재분화 효율은 4주령 캘러스에서 그 범위가 6.8~11.5%로서 Jangbeol 102가 가장 높았으며, 6주령 캘러스에서는 범위가 7.2~25.6%로서 Potomac이 가장 높았다.

Keywords

References

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