• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.1
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• pp.53-61
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• 1985

Effects of nitrogen incorporated with 15N-nitrogen, which was applied to grown alone and in mixture with grass and legumes of forage crops, on the yield of dry matter, total nitrogen content, percentage of nitrogen derived from fertilizer (P.N.D.F.), A-value were studied, and the results obtained are as follows: 1. With nitrogen for the yield of dry matter was highly effective only to orchardgrass and were also effect to the early stages of alfalfa and birdsfoot trefoil in case of a mixed sowing. 2. Alone and mixed sowings showed the yield of dry matter in decreasing order of alfalfa<red clover>orchardgrass>birdsfoot trefoil, and orchardgrass + alfalfa>orchardgrass + red clover>orchardgrass + birdsfoot trefoil, respectively. 3. In case of grown alone, orchardgrass gave higher percentage of nitrogen derived from fertilizer (P.N.D.F.) than legume, whereas in case of grown in mixture it was in reverse order throughout the plots. It appears likely that orchardgrass was rather supplied soil nitrogen by legume then by nitrogen application. And P.N.D.F. was getting smaller in the latter stage of all forage crops in case of grown alone. In case of grown in mixture, however, and inconsistent P.N.D.F. was obtained from orchardgrass. 4. The A-value in case of grown alone was decreased in order of alfalfa>red clover>orchardgrass>birdsfoot trefoil. In contrast, however, the A-value in case of grown in mixture wes decreased in order of orchardgasss+alfalfa (3:7)>orchardgrass+red clover (3:7)>orchardgrass+red clover (3:7)>orchardgrass+alfalfa(5:5)>orchardgrass+birdsfoot trefoil (5:5)>orchardgrass+red clover (5:5)>orchardgrass+birdsfoot trefoil (3:7).

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.630-635
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• 2000

Previous research has not evaluated the effects of various rates and frequencies of manure application and frequencies of cutting on yield and stand persistence of cool-season grasses and alfalfa-grass mixtures. The primary objective of this study was to compare the effects of cutting management systems on herbage yield and stand persistence of orchardgrass (Dactylis glomerata L.) and an alfalfa (Medicago sativa L.)-orchardgrass mixture from various rates and frequencies of dairy slurry application. A randomized complete block design with treatments in a sub-subplot arrangement with four replicates was used. The main plot consisted of 2 cutting management systems (4 and 5 annual cuttings). The subplots were 9 fertility treatments: 7 slurry rate and frequency of application treatments, one inorganic fertilizer treatment, and an unfertilized control. The split-split-plots were the two forage species: orchardgrass and alfalfa-orchardgrass mixture. The study was initiated after 1st cutting in 1995. Cumulative yields of the 2nd and subsequent cuttings of both orchardgrass and alfalfa-orchardgrass in 1995 were higher for the 5-cutting system than the 4-cutting system. The 1995 growing season was abnormally dry. In 1996, an abnormally wet year, the reverse was true, total herbage yields being higher for the 4-cutting system than the 5-cutting system. Species response to fertility rate/frequency treatments was different in both years. Higher application rates early in the season and carryover of nutrients from late season applications the previous year appear to be responsible for the yield increases of those fertility treatments having significant yield differences between the cutting management systems. The stand ratings of orchardgrass were not affected by cutting management. In the spring of 1997, however, the stand ratings of alfalfa-orchardgrass in the 4-cutting management system were significantly greater than the 5-cutting management system. The very high manure application rate significantly reduced the stand ratings of alfalfa-orchardgrass in the 5-cutting system.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.2
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• pp.93-100
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• 1995

This experiment was carried out to investigate how to effect the application of nitrogen, phosphate and potassium on the vegetation and dry matter yield in the mixed sward of orchardgrass and crabgrass (Digitaria sanguinalis [L.] Scop.). The experimental sward wa5 consisted of eight plots of non-application, P, K, PK, N. NK, NP and NPK. These plots were sown with seeds mixture of orchardgrass 17.5 kglha and crabgrass 17.5 kg/ ha on 22 April 1975 in the forage experimental field of Kangwon National University, Chunchon, Korea, and were cut four times from 6 July to 18 November 1975. The results obtained were summarized as follows: 1. In orchardgrass density measured after summering, the plot of nitrogen applied without phosphate marked only 3 1.0% of the plot of nitrogen applied with phosphate while in crabgrass density measured at that time the plot of nitrogen applied without phosphate marked as high as 112.5% of the plot of nitrogen applied with phosphate. 2. Application of phosphate and potassium improved wintering record of orchardgrass. In orchardgrass density measured after wintering, the plot of nitrogen applied without phosphate marked only 9.7% of the plot of nitrogen applied with phosphate, and the plot of NP applied without K marked 63.6% of the plot of NP applied with K. 3. In surface coverage of orchardgrass, in midsummer any plot applied with nitrogen regardless of accompanying with or not with phosphate wa5 0%, but in autumn the plot of nitrogen applied with phosphate was 56-58% and the plot of nitrogen applied without phosphate was 5%. While after wintering the plot of nitrogen applied with phosphate was 28-37% though the plot of nitrogen applied without phosphate was only 0.3-0.6%; the application of phosphate improved summering and wintering record of orchardgrass. 4. Application of phosphate demonstrated remarkable effect on increasing dry matter yield in orchardgrass. In year total yield of orchardgrass, the plot of nitrogen applied without phosphate marked as low as 11.3% of the plot of nitrogen applied with phosphate, but in crabgrass the plot of nitrogen applied without phosphate was almost equal to the plot of nitrogen applied with phosphate showing 97.5% of this plot. 5. Application of potassium was not effective on increasing dry matter yield of orchardgrass but its application was effective on increasing dry matter yield of crabgrass. In dry matter yield of orchardgrass significant difference was not found between NP plot and NPK plot, but in dry matter yield of crabgrass NPK plot recorded significantly higher yield compared with NP plot (p<0.05). 6. In botanical composition of the plot of nitrogen applied with phosphate, crabgrass recorded 46.5-50.5% in early summer (July) but in midsummer (August) it dominated the plot recording 85.6-91.2%. 7. In botanical composition based on the year total yield, in the plot of nitrogen applied with phosphate orchardgrass marked 21.7-26.2% and crabgrass recorded 73.8-78.370, but in the plot of nitrogen applied without phosphate orchardgrass marked only 3.5% being overwhelmingly dominated by crabgrass which recorded 96.5%. 8. Application of nitrogen not accompanied with phosphate strengthened competitive power of the crabgrass unilaterally, making orchardgrass be oppressed fatally by the crabgrass.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.3
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• pp.137-144
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• 2001

The quality including in situ degradability in the rumen of Holstein of the orchardgrass silage added with apple pomace was investigated in this study. The amount of apple pomace added in different treatments were 0, 20, 40 and 60% respectively. With higher amount of addition of apple pomace to orchardgrass, ADF, NDF and crude ash contents decreased significantly (p<0.05). Crude protein contents in the silages (11.8- 12.9%) were similar to that of 100% orchardgrass silage. Moisture contents increased according to the higher proportion of apple pomace in the silages. On the contrary to moisture content, pH was lower in 40-60% addition of apple pomace (3.7-3.9) than that of 100% orchardgrass silage (4.7). However the contents of lactic acid (1.7-2.5%), acetic acid (1.3- 1.7%) and total organic acid (2.9-4.2%) significantly increased according to higher levels of addition of apple pomace compared to the respective values of 100% orchardgrass silage (1.1%, 0.6% 1.7%). In siru disappearance rates of dry matter and NDF in the rumen were significantly higher at the stages of incubation after 24h in 40-60% addition of apple pomace than in 100% orchardgrass silage. No statistical differences were observed with quickly degradable fraction (a) and slowly degradable fraction (b) in the disappearance rates of dry matter and NDF. However, fractional rate of disappearance (c) and effective degradability (ED, k=0.08) for dry matter and NDF were significantly higher in 20-60% addition of apple pomace as 0.0076-0.0079 and 0.0099-0.0130. and 39.3-41.7% and 18.4- 20.6% respectively than the respective values of 0.0054 and 0.0064, and 36.8 and 16.5% of 100% orchardgrass silage. (Key words : Orchardgrass silage. Apple pomace. Lactic acid, In Situ Degradability. Effective degradability)

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.2
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• pp.103-108
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• 2000

To produce of transgenic orchardgrass, the seed-derived calli of orchardgrass (Dactylis glomerata L.) co-cultivated with Agrobacterium turnefaciens EHAlOl harboring binary vector pIG121-Hm were selected with hygromycin and then transferred onto N6 regeneration medium containing 1 rngl l of NAA, 5 rngl l of kinetin, 250 rngl l of carbenicillin and 50 mg/ l of hygromycin. The efficiency of transformation was differed on cultivars, that is, 'Potomac' appeared 12% of transformation efficiency while 'Amba' did 5.5%. The addition of acetosyringone during co-cultivation was a key to successhl transformation of orchardgrass. Transgene fragments were identified by PCR analysis and the constitutive expression of GUS gene was confirmed by Northern blot analysis. (Key words : Acetosyringone, Agrobacterium tumefaciens, Orchardgrass (Dactylis glomerata L.), Transformation)

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.1
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• pp.21-26
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• 2001

To develop transgenic orchardgrass resistant to reactive oxygen species produced from environmental stresses, a vector with the cytosolic glutathione reductase cDNA (BcGRl) from Chinese cabbage was constructed under the control of the cauliflower mosaic virus 35S promoter and was introduced into orchardgrass using Agrobacterium tumefaciens EHA101. Transgenic plants from hygromycin-selected calli of orchardgrass did not show any morphological difference from wild-type plants. The results of PCR amplification and genomic Southern blot analysis confirmed the integration of foreign gene into the chromosome of transgenic orchardgrass. Northern blot analysis with total RNA from leaves also confirmed the constitutive expression of BcGR1 in transgenic orchardgrass.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.4
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• pp.203-208
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• 1991

This experiment was carried out to compare the effects of summer temperature and nitrogen application on carbohydrates and fiber contents in orchardrass stubble grown in the cool summer year, 1980 and in the hot summer year, 1984. For this study, the contents of carbohydrates, neutral detergent fiber (NDF), acid detergent fiber(ADF) and hemicellulose in orchardgrass stubble were investigated. 1. Total water soluble carbohydrates(TSC) in orchardrass stubble were accumulated during the period of cool season and were declined when the weather was hot and dry. The reserve level of those were decreased when a new leaf emerged after the start of plant growth and then were increased as the plant was in full flourish. 2. The reserve of carbohydrate content in orchardgrass stubble was about twice times higher in cool summer year than that in hot summer year, and the reserve rate of carbohydrates showed to be fast in cool summer year. When the nitrogen fertilizer applied, the content of TSC in orchardgrass stubble more lowered but not significantly different(p<0.05). 3. The contents of NDF, ADF and hemicellulose in orchardgrass stubble in cool summer year were not significantly different from those in hot summer year. These contents showed also no significant difference among different levels of nitrogen applications. 4. The relationship of carbohydrate, NDF, ADF and hemicelluose contents according to the levels of nitrogen applications were negatively correlated in cool summer year. But there was no consistency in hot summer year.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.1
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• pp.50-55
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• 1983

Effects of nitrogen applied to grown alone and in mixture with grass and legumes of forage crops on the yield of dry matter, absorptivity, and the amount of nitrogen fixation were studied using $^{15}N$ ammonium sulfate. 1. The amount of nitrogen fixation in legume was decreased in order of alfalfa > red clover > birdsfoot trefoil, and red clover gave highest soil-nitrogen dependency. 2. Fertilizer nitrogen for the yield of dry matter was highly effective only in orchardgrass, and effective at the early stages of alfalfa and birdsfoot trefoil in case of a mixed sowing. 3. Alone and mixed sewings showed the yield of dry matter in decreasing order of alfalfa > red clover > orchardgrass > birdsfoot trefoil and orchardgrass + alfalfa > orchardgrass + red clover > orchardgrass + birdsfoot trefoil, respectively. 4. The plot of with nitrogen showed greater nitrogen uptake than the plot of without-nitrogen throughout the plots. In case of grown alone, however, difference in the absorptivity of various forage crops was decreased in order of alfalfa > red clover > orchardgrass > birdsfoot trefoil. 5. Regardless of nitrogen application, nitrogen uptake amount of orchardgrass was higher in the plot of mixture then in the plot grown alone, except the with nitrogen plot of red clover grown in mixture at the ratio of 3 : 7.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.4
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• pp.222-229
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• 1991

This experiment was carried out to determine the effect of improvement of oversown pastures, treated with four nitrogen(N) application levels(0, 100, 200, 300 kg/ha) and three species mixtures (A: orchardgrass+ladino clover; B: orchardgrass+ladino clover+perennial ryegrass; C: orchardgrass+ladino clover+perennial ryegrass+Italian ryegrass). The establishment of perennial ryegrass, Italian ryegrass, and ladino clover were increased with increased N applications, but the ryegrass mixed with orchardgrass and ladino clover decreased the establishment of ladino clover. With the increase of N application levels, orchardgrass, perennial ryegrass and Italian ryegrass in comparison on botanical composition were getting better, while the native plants established already were decreased. The botanical composition of newly sown pasture plants was increased as cutting times progressed. The dry matter(DM) yield of pasture species increased significantly with N application up to 300 kg/ha. There was no significance among mixture treatments, although the DM yield of basic treatment(orchardgrass+ladino clover) was higher than other treatments. However, when compared DM yields on each cutting times, newly oversown pastures in A treatment was shown to be significantly higher only on 2nd cutting time than other treatments(P<0.05). The DM yield of native plants survived in basic species mixture was significantly less than those of other mixtures(P<0.05). Results of this study indicate that for improving oversown pastures, at least 300 kg/ha N application should be applied, and basic mixture (orchardgrass+ladino clover) has more advantage than treatments mixed with ryegrass.

• Journal of the Korea Organic Resources Recycling Association
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• v.2 no.2
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• pp.77-88
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• 1994

This experiment was carried out to investigate the effect of sewage sludge compost amended soils on the growth and accumulation patterns of heavy metals in plant parts of Orchardgrass seedlings, changes in physical properties and chemical composition, and heavy metal residue in soils. Mixture ratios of sewage sludge compost and soil(loam) were 100:0, 80:20, 60:40, 40:60, 20:80 and 0:100(control), respectively. The results obtained were as follows; 1. The physical properties and chemical compostion of soils were improved by increase in mixture ratios of sewage sludge compost. 2. The biological yield of Orchardgrass seedlings was increased with mixture ratios of sewage sludge compost. 3. The dry weight of shoot(SH) was increased with both of yield components(NT and WT) and biological yield of Orchardgrass seedlings. 4. The total nitrogen concentrations(TN) of plants was increased with quadratically up to the biological yield of 100% mixture ratio of sewage sludge compost. 5. Lead(Pb) concentration of soil in over the 60% mixture ratios of sewage sludge compost were in excess of limiting level(50ppm) of organic fertilizers.