The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Effect of Injin Butanol Fraction with Thin Layer Chromatography on Fas-mediated Apoptosis

인진butanol 분획의 TLC추출성분이 Fas-mediated Apoptosis에 미치는 영향

  • 박용진 (경희대학교 한의과대학 간계내과학교실) ;
  • 김영철 (경희대학교 한의과대학 간계내과학교실) ;
  • 이장훈 (경희대학교 한의과대학 간계내과학교실) ;
  • 우흥정 (경희대학교 한의과대학 간계내과학교실)
  • Published : 2002.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: The aim of this study is to investigate the effect of Injin butanol fractions with Thin Layer Chromatography on Fas-mediated Apoptosis. Method: Injin-butanol fraction separated by TLC. MIT assay, cell cycle analysis, Caspase-3 protease assay, DNA fragmentation assay and quantitative RT-PCR were performed to evaluate the effects of TLC extraction of lnjin-butanol fraction on cell viability, cell cycle progression and apoptosis. Results: Scopoletin, luteolin, apigenin and unknown powder was isolated by TLC. Fas-mediated apoptosis analysis shows that scopoletin has inhibiting function on apoptosis. Caspase- 3 protease assay analysis shows that scopoletin inhibits activity of caspase-3. Quantitative RT-PCR analysis shows that no activity on caspase-3, but apoptosis inhibition cytokine -Bcl-2- is activated, and apoptosis activating cytokine -Bax- is unactivated. Conclusion: These results show that each fraction of Injin-butanol TLC extraction, especially scopoletin, acts as a protective function on liver cell viability, and inhibitory function on apoptosis. (J Korean Oriental Moo 2002;23(2):57-69)

Keywords

References

  1. 金궤要略 張仲景
  2. 茵陳五령散과 茵陳增量한 構成方이 흰쥐 손상간에 미치는 영향 우홍정
  3. 茵陳과 茵陳四령散加減方이 간세포활성, 세포주기 및 DNA damage-induced apoptosis에 미치는 영향 강우성
  4. 茵陳四령散이 간세포활성, 세포주기 및 Fas-mediated apoptosis에 미치는 영향 표임정
  5. 대한한의학회지 v.18 no.1 인진과 갈근이 d-galactosamine, 급성 alcohol중독 및 $CCl_4$중독 백서의 간손상에 미치는 영향 우홍정;이장훈;김영철
  6. 1999년도 사망원인통계결과 통계청
  7. 仲景全書 張仲景
  8. 간계내과학 전국 한의과대학 간계내과학교실
  9. 第2回 韓$\cdot$中 學術大會 參加論文集 -肝臟編 간질환치료제의 효능에 관한 실험적 연구 이장훈
  10. 茵陳淸肝湯의 안전성에 관한 연구 김영철
  11. The Korean Journal of Hepatology v.2 no.2 아포토시스와 간질환 김창민
  12. Br. J. cancer. v.26 Apoptosis. a basic biological phenomenon with wideranging implications in tissue kinetics Kerr JF;Willie AH;Currie AR
  13. C. J. Hepatology v.19 no.6 Immunohistochemical detection of Fas antigen in liver tissue of patients with chronic hepatitis Hiramatsu N.;Hayashi N.;Katayama K.;Mochizuki K;Kawanishi Y.;Kasahara A.;Fusamoto H.;Kamada T.
  14. The Graduate School of Kyung-hee University. The effect of Inchinchunggantang-derivative on Proliferation of Hepatocyte 박용진
  15. 茵陳淸肝湯加減方이 간세포활성, 세포주기 및 apoptosis에 미치는 영향 홍상훈
  16. 茵陳분획물이 간세포활성, 세포주기 및 Fas-mediated apoptosis에 미치는 영향 이종훈
  17. 茵陳분획물이 인체간세포의 $TGF{\beta}1$-induced apoptosis에 미치는 영향 이지현
  18. 茵陳四笭散 분획물이 간세포활성, 세포주기 및 Fas-mediated apoptosis에 미치는 영향 고흥
  19. 대한약전 및 대한약전외 한약규격주해 지형준
  20. Pusan Bull. Pharm. Sci. v.26 Studies on the constituents of Artemisia iwayomogi Bae JM;Rho YJ;Young HS;Choi JS;Park HJ;Park JC
  21. Kor. J. Pharmacogn v.24 Studies on the constituents of Impatiens textori Kim JW;Choi KS
  22. J. Korean Soc. Food Nutr. v.21 The pharmaco-chemical study on the plant of Ixeris spp. 2. Flavonoids and free amino acid composition of Ixeris sonchifolia Young HS;Im KS;Choi JS
  23. Kor. J. Pharmaogn. v.25 Flavonoids from Codonopsis lanceolata leaves Whang WK;Park KY;Chung SH;Oh IS;Kim IH
  24. 원색한국본초도감 안덕균
  25. 소화기계 질환 김정룡(편저)
  26. Journal of immunologic methods v.65 no.1;2 Rapid colorimetric assay for cellular growth and survival. application to proliferation and cytotoxicity assays Mosmann T
  27. Cancer chemother Pharmacology v.17 no.3 Rapid chemosensitivity testing of human lung tumor cells using the MTT assay Cole S. P.