• The Korean Journal of Mycology
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• v.29 no.1
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• pp.67-71
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• 2001

A steroid compound, F-l with antioxidative activity from the fruit bodies of Fomitella fraxinea was isolated by ethyl acetate extraction, silica gel column chromatography, and preparative silica thin layer chromatography. The structure of the compound was determined to be crgosta-7,22-diene-3-one-$16{\beta}-ol$ by IR, NMR, and GC-Mass. The compound exhibited inhibition on lipid peroxidation of rat liver microsomes, with $IC_{50}$ value of $3.8{\mu}g/ml$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.5
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• pp.446-452
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• 1986

This paper presents the composition of neutral and polar lipids obtained from puple shell, Rapana venosa and the abalone, Haliotis discus hannai. The fatty acid composition and the classification of neutral lipids from two species were determined by gas chromatography (GLC) and thin layer chromatography (TLC). Total lipid contents of samples were $0.5\%$ in purple shell and $0.4\%$ in the abalone. The predominant fatty acids of total lipids were eicosapentaenoic acid ($19.30\%$). eicosenoic acid ($12.10\%$) and palmitic acid ($11.77\%$) in the purple shell, and palmitic acid ($21.29\%$), oleic acid ($14.55\%$) and linoleic acid ($14.21\%$) in the abalone. The lipid composition of non-polar lipid fractions in purple shell and abalone was separated and identified as free sterol, free fatty acid, triglyceride and hydrocarbon & esterified sterol by TLC. The contents of triglyceride from both neutral lipids were shown more abundant than any other subclasses. The main fatty acids of neutral lipids were eicosapentaenoic acid ($18.6\%$), palmitic acid ($14.90\%$) and eicosenoic acid ($14.76\%$) in the purple shell, and palmitic acid ($28.12\%$), oleic acid($20.5\%$) and myristic acid ($12.5\%$) in the abalone. Eicosapentaenoic acid ($17.57\%$), stearic acid ($13.26\%$) and eicosatetraenoic acid ($11.24\%$) were important fatty acids of glycolipid in the purple shell, and myristic acid ($12.75\%$), stearic acid ($12.10\%$) and eicosatetraenoic acid ($10.64\%$) in the abalone. The major fatty acids of phospholipids were eicosapentaenoic acid ($20.18\%$), palmitic acid ($11.26\%$) and eicosenoic acid ($10.90\%$) in the purple shell, and palmitic acid ($21.10\%$), eicosapentaenoic acid ($12.90\%$) and oleic acid($11.13\%$) in the abalone.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.4
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• pp.333-343
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• 1984

This study was designed to elucidate the lipid and its fatty acid composition in various tissues of fresh water fishes. The free and bound lipids in meat, skin and viscera of crucian carp (Carassius carassius) were extracted with ethyl ether and the mixed solvent of chloroform-methanol-water (10/9/1, v/v). The free and bound lipids were fractionated into neutral lipid, glycolipid and phospholipid by a silicic acid column chromatography using chloroform, acetone and methanol, respectively, and quantitatively analyzed by thin layer chromatography (TLC) and TLC scanner. The fatty acid compositions of polar ana nonpolar lipids in meat, and these of neutral lipid in various tissues were analyzed by gas liquid chromatography(GLC). The free lipid content in meat, skin and viscera was $6.22\%,\;9.95\%\;and\;9.76\%$, whereas the bound lipid content in those tissues was $10.01\%,\;3.56\%\;and\;7.36\%$, respectively. The neutral lipid contents in free lipid were ranged from $71.7\%$ to $89.4\%$, and $3{\sim}9$ times higher than those in bound lipid, while the phospholipid contents in bound lipid were ranged from $42.3\%$ to $63.2\%$, and $5{\sim}10$ times higher than those in free lipid. The neutral lipid was mainly consisted of triglyceride ($81.91{\sim}88.34\%$) in free lipid, and esterified sterol & hydrocarbon ($41.00{\sim}59.43\%$) in bound lipid. The phospholipid was mainly consisted of phosphatidyl ethanolamine($54.56{\sim}66.79\%$) and phosphatidyl choline ($21.88{\sim}34.28\%$) in free lipid, and phosphatidyl choline ($50.49{\sim}70.57\%$) and phosphatidyl ethanolamine ($15.74{\sim}24.92\%$) in bound lipid. The major fatty acids of polar lipid in free and bound lipids were $C_{16:0}\;(17.53\%,\;19.29\%)$, $C_{18:1}\;(24.57\%,\;16.08\%)$, $C_{18:2}\;(8.39\%,\;4.03\%)$, $C_{22:5}\;(1.68\%,\;8.08\%)$, and $C_{22:6}\;(6.22\%,\;13.60\%)$ and these of neutral lipid in free and bound lipids were $C_{16:0}\;(17.67\%,\;24.15\%)$, $C_{16:1}\;(12.81\%,\;5.52\%)$, $C_{18:1}\;(24.13\%,\;13.02\%)$, $C_{18:2}\;(15.47\%,\;8.68\%)$, $C_{22:5}\;(0.88\%,\;4.14\%)$ and $C_{22:6}\;(1.17\%,\;5.04\%)$, respectively. The unsaturations (TUFA/TSFA) of polar lipid in free and bound lipids were 2.02 and 2.74, and $1.5{\sim}2.0$ times higher than 1.51 and 1.23 of nonpolar lipid. In both polar and nonpolar lipids, w3 highly unsaturated fatty acid (w3HUFA) content of bound lipid was $2{\sim}5$ times higher than that of free lipid. The polyenoic acid contents such as $C_{20:5},\;C_{22:5}\;and\;C_{22:6}$ in bound lipid were $2{\sim}5$ times higher than these in free lipid. Consequently, there were significant difference between the lipid and its fatty acid composition in free and bound lipids and/or in various tissues.

• Applied Chemistry for Engineering
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• v.29 no.3
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• pp.342-349
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• 2018

In this study, the physicochemical properties, emulsifying capacity, moisture content and cytotoxicity of the composite material produced by transesterification reactions of the olive oil (olive oil esters) were investigated for cosmetic applications. Olive oil esters with short (S) and long (L) reaction times were studied. From the TLC-image analysis, composition ratios of the olive oil esters S were found to be 5.2, 24.1, 46.4, and 21.9% for mono-, di-, tri-glyceride, and fatty acid ethyl ester, respectively. Those of the olive oil esters L were 4.1, 24.7, 40.6, and 28.8% for mono-, di-, tri-glyceride, and fatty acid ethyl ester, respectively. The iodine value, acid value, saponification value, unsaponified matter, refractive index, and specific gravity were determined and purity tests were also carried out and normalized to establish standards and testing methods for using olive oil esters in cosmetics. To evaluate their emulsifying capacities, the O/W emulsion was prepared without surfactants and the formation of the emulsified particles were confirmed. After 5 days of applying the olive oil esters to human skin, the skin moisture retention was improved by 13.1% from the initial state. For the evaluation of toxicity on human skin cells, the olive oil esters showed 90% or more of the cell viability at $0.2-200{\mu}g/mL$. These results suggested that olive oil esters can be applied as natural/non-toxic ingredients to cosmetics industries.