• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.24 no.3
• /
• pp.96-104
• /
• 1998

It was possible to produce W/O/W one step multiple emulsion on the system which satisfied following conditions. 1. 1-5％ of hydrophilic liquid surfactant over HLB20 and lipophilic liquid surfactant which has HLB 3∼5 2. Non wax copolymers as oil thickener 3. More than 0.5％ of carbomer as aqueous thickener 4. The manufacturing process which neutralize the dispersed carbomer (2.0％ in water), after emulsifying. For the selective addition into inner and outer aqueous phase, we melted the glucose in water before emulsifying. Using an Anthrone analysis method, we analyzed the encapsulation yield of glucose in inner water phase. It was possible to raise the water encapsulation yield of the multiple emulsion through the following conditions. 1. Using of anionic hydrophilic surfactant(HLB 40) and lipophilic surfactant (HLB 3∼5) 2. Controlling the ratio of hydrophilic surfactant and lipophilic surfactant 3. Strengthening interface with increase of non wax oil thickener. When the separated adding process of glucose was adopted, approximately 85％ of glucose was added selectively within inner aqueous phase.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.4
• /
• pp.452-458
• /
• 2015

The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35℃, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12-ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicum-based biocatalyst.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.20 no.6
• /
• pp.603-608
• /
• 1991

To improve the utilization of red pepper, the extracting conditions of oleoresin, such as kind of solvents, particle sizes of a sample, sample to solvent ratios, extraction temperatures and times, were studied. Among eight solvents used for oleoresin extraction from red pepper, the optimal solvent was acetone. The most appropriate particle size of red pepper powder, extracting temperature and mixing ratio of red pepper to acetone were 100 mesh, $25^{\circ}C$ and 1 to 3(w/w), respectively. The basis of yield in oleoresin extraction, optimum extracting time was about 5 hours. The yield of oleoresin under the above-mentioned conditions was 18.7%.

• Korean Journal of Soil Science and Fertilizer
• /
• v.49 no.5
• /
• pp.461-468
• /
• 2016

One of the important phytohormones produced by plant growth promoting bacteria is the auxin; indole-3-acetic acid (IAA), with L-tryptophan as the precursor. In this study, we focused on the investigation of optimal conditions for the production of IAA by Bacillus megaterium BM5. We investigated culturing conditions, such as incubation temperature, pH of the culture medium and incubation period, with varying media components such as inoculation volume, tryptophan concentration and carbon and nitrogen source. Besides, optimization study intended for high IAA production was carried out with fermentation parameters such as rpm and aeration. The initial yield of $42{\mu}g\;IAA\;ml^{-1}$ after 24 hr increased to $85{\mu}g\;ml^{-1}$ when 5% (v/v) of L-tryptophan was used in the culture broth. The maximum yield of $320{\mu}g\;IAA\;ml^{-1}$ was observed in trypticase soy broth (TSB) supplemented with starch and soybean meal as C and N sources with a C/N ratio of 3:1 (v/v) at $30^{\circ}C$, pH 8.0 for 48 hrs with 1.0 vvm and 250 rpm in 5 L working volume using 10 L scale fermenter. The bacterial auxin extracted from the culture broth was confirmed by thin layer chromatography and high-performance liquid chromatography and effect on plant growth was confirmed by root elongation test.

• Journal of Microbiology and Biotechnology
• /
• v.22 no.7
• /
• pp.939-946
• /
• 2012

Antibiotic production with Streptomyces sindenensis MTCC 8122 was optimized under submerged fermentation conditions by artificial neural network (ANN) coupled with genetic algorithm (GA) and Nelder-Mead downhill simplex (NMDS). Feed forward back-propagation ANN was trained to establish the mathematical relationship among the medium components and length of incubation period for achieving maximum antibiotic yield. The optimization strategy involved growing the culture with varying concentrations of various medium components for different incubation periods. Under non-optimized condition, antibiotic production was found to be $95{\mu}g/ml$, which nearly doubled ($176{\mu}g/ml$) with the ANN-GA optimization. ANN-NMDS optimization was found to be more efficacious, and maximum antibiotic production ($197{\mu}g/ml$) was obtained by cultivating the cells with (g/l) fructose 2.7602, $MgSO_4$ 1.2369, $(NH_4)_2PO_4$ 0.2742, DL-threonine 3.069%, and soyabean meal 1.952%, for 9.8531 days of incubation, which was roughly 12% higher than the yield obtained by ANN coupled with GA under the same conditions.

• Textile Coloration and Finishing
• /
• v.24 no.4
• /
• pp.253-259
• /
• 2012

In this work, the natural indigo production process from Polygonum tinctorium was balanced based on the traditional Niram method in Korea. A standard procedure was determined considering the conditions of indican extraction from plant material, the amount of alkali for precipitation, storage of extract, etc. The effect of experimental conditions on the yield of crude dye was investigated. The contents of indigo and indirubin of the crude dyes were analyzed by HPLC. Increase of the amount of crude dye was observed within 1-2.5 days of extraction time. Longer extraction beyond 2.5 days resulted in a slight decrease in the amount of crude dye. There was no consistency in terms of indigo content depending on extraction pH. We found that the storage of extract or harvested plants affected adversely to dye yield and dye quality. Based on the lab scale extraction, large scale extraction was performed for 2-2.5 days in water and 2.0-2.5 g/L of $Ca(OH)_2$ was applied for precipitation of indigo dye. We obtained natural indigo dye containing about 15% of pure indigo in scale-up production using whole plant except root.

• Journal of Korean Society of Steel Construction
• /
• v.19 no.2
• /
• pp.181-190
• /
• 2007

A fire-resistant steel with enhanced load-bearing capacity has been developed to enable structural elements such as columns and beams withstand exposure to severe fire conditions. To precisely evaluate the fire-resistant performance of structural elements that compose fire-resistant steels, mechanical properties such as yield strength and elastic modulus are essential. To obtain the mechanical database of fire-resistant steels at high temperatures, tensile tests at high temperatures were conducted on steels of two kinds of thicknesses. The results showed that the thickness difference could not affect the mechanical properties at a high temperature.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.22 no.2
• /
• pp.45-49
• /
• 2011

The performance of five newly evolved cross breeds were evaluated across seasons to understand the genotype and environment interactions and their stability under fluctuating tropical environmental conditions along with control hybrids $PM{\times}CSR2$ and $APM1{\times}APS8$. The data was collected on eight economic traits namely fecundity, yield per 10,000 larvae by number, yield per 10,000 larvae by weight, survival rate, cocoon weight, cocoon shell weight, cocoon shell ratio and filament length. The data was subjected for Analysis of Variance (ANOVA) and relevant statistical methods. The performance data obtained was further subjected for multiple trait evaluation index method. The ranks were adjudicated based on the index values. The two hybrids viz., $MSO3{\times}APS45$ and $MSO1{\times}APS12$ performed well under diversified environmental conditions indicating their overall stability and superiority. These hybrids revealed highly significant (p < 0.01) improvement for the majority of the traits over the control hybrids.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.17 no.2
• /
• pp.156-164
• /
• 2015

High temperature stress would affect rice production in the future as heat wave is expected to occur frequently under climate change conditions. The objective of this study was to obtain rudimentary information to assess the impact of heat stress on rice yield and its yield component in Korea. Two rice cultivars "Hwaseongbyeo" (Japonica) and "Dasanbyeo" (Tongil-type) were grown at different nitrogen fertilization levels in two seasons. These cultivars were grown in 1/5000a Wagner pot placed within four plastic houses where temperature was controlled at ambient, ambient$+1.5^{\circ}C$, ambient$+3^{\circ}C$ and ambient$+5^{\circ}C$ throughout the rice growing season in Suwon ($37^{\circ}16^{\prime}N$, $128^{\circ}59^{\prime}E$), Korea. The degree of temperature change affected grain yield whereas the level of nitrogen had little impact on grain yield. The number of panicle per pot and spikelet per panicle were not significantly different among temperature treatments in both cultivars tested. In contrast, 1000-grain weight and ripened grain ratio were decreased significantly under the treatments raising the air temperature to the level of $5.0^{\circ}C$ and $1.5^{\circ}C$ above the ambient air temperature in Dasanbyeo and Hwaseongbyeo, respectively. Reduction of 1000-grain weight and ripened grain ratio under the temperature treatments of $3.0^{\circ}C$ and $5.0^{\circ}C$ above the ambient air temperature resulted in significantly less grain yield for Dasanbyeo and Hwaseongbyeo, respectively. The greater sensitivity of grain yield to temperature increase in Dasanbyeo was attributable to the sharp decrease of 1000-grain weight and ripened grain ratio with the temperature rise above $23^{\circ}C$ during ripening period. On the other hand, Hwaseongbyeo had little variation of them in the temperature range of $23-27^{\circ}C$. These results suggested that grain yield would decrease under future climate conditions due to grain weight decreased by shorter grain filling period as well as the ripened grain ratio reduced by spikelet sterility and early abortion of rice kernel development. Thus, it would be essential to use cultivars tolerant to heat stress for climate change adaptation, which merits further studies for developing varieties that have traits to avoid spikelet sterility and early abortion of rice kernel, e.g., early morning flowering, under heat wave.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.323-323
• /
• 2017

In many sub-Saharan African countries, boosting rice production is a pressing food security issue. To contribute to the increase in rice production, we have developed lines of NERICA 1 introgressed with the gene for spikelet number, Gn1a, and the gene for primary rachis-branch number, WFP by cross breeding. The performance of rice lines introgressed with the genes for yield related traits can be affected by cultivation environment and management. Thus, in this study, we aimed to evaluate the lines of NERICA 1 introgressed with Gn1a or/and WFP for yield and yield components under different nitrogen fertilization conditions in Kenya. A field trial was conducted at a paddy field in Kenya Agricultural and Livestock Research Organization-Mwea, Kirinyaga County ($0^{\circ}39^{\prime}S$, $0^{\circ}20^{\prime}E$) from August 2016 to January 2017. Eight lines of NERICA 1 introgressed with Gn1a and/or WFP, and their parents, NERICA 1 and ST12, were grown under 0 (NF) and $75(SF)kg\;N\;ha^{-1}$. At maturity, five hills per plot were harvested to determine the yield and yield components. The number of primary and secondary rachis-branches per panicle was measured on the longest panicle in each hill. Under SF, the introgression of WFP to NERICA 1 increased the number of primary and secondary rachis-branches by 27 and 25%, respectively. On the other hand, Gn1a did not increase the number of primary rachis-branches, whereas the number of secondary rachis-branches was increased by 38% on average. The number of primary and secondary rachis-branches of the lines introgressed with both genes increased by 25 and 56%, respectively. Although grain number per panicle increased 33% by Gn1a, 34% by WFP, and 43% by Gn1a+WFP, the yield increase by Gn1a, WFP, and Gn1a+WFP was only 14, 7, and 14%, respectively. The suppression of the yield increase was mainly attributed to the decline in the filled grain ratio. Under NF, WFP increased the number of primary and secondary rachis-branches by 20 and 19%, respectively. The introgression of both genes increased the former and the later by 19 and 35%, respectively. However, Gn1a did not change them under NF. Thus, even under NF, grain yield increased 11% by WFP and 24% by Gn1a+WFP due to the increased grain number although filled grain ratio declined. Our findings suggest that the introgression of Gn1a and WFP could contribute to the rice productivity improvement in sub-Saharan Africa even under low fertility conditions. Improving filled grain ratio of the lines introgressed with these genes by further breeding and fertilization management will be the focus of subsequent work.