• Journal of Mushroom
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• v.12 no.3
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• pp.181-186
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• 2014

This research was carried out to analyze the genetic variation of 18 wild strain, 2 breed varieties and 20 mutants of Hypsizygus marmoreus by random amplification of polymorphic DNA(RAPD). Also, 3 strains of Lyophyllum decartes and 1 strain of Lyophyllum shimeji were used. These mushrooms were collected from korea, china, Taiwan and Japan. Spores of H. marmoreus JV-2 strain were irradiated by gamma ray for mutagenesis. 40 kind of primers were used for this reaserch. Number of reaction primer were 31. Electrophorectic patterns of RAPD showed genetic variation. In phylogenetic tree, they were divided into seven group. Discriminative differences were observed between wild strain and mutants in H. marmoreus. These results might suggest that these primers and gamma ray irradiation of spores were useful tools for developing new strain for mushroom.

• Journal of Microbiology and Biotechnology
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• v.21 no.3
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• pp.256-262
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• 2011

A 20.9-kDa metalloprotease was isolated from dried fruiting bodies of the wild basidiomycete mushroom Lepista nuda. The N-terminal amino acid sequence of the protease was seen to be ATFVLTAATNTLFTA, thus displaying no similarity with the sequences of previously reported metalloproteases. The protease was purified using a procedure that entailed ion-exchange chromatography on CM-Cellulose, Q-Sepharose, and Mono S, and FPLC-gel filtration on Superdex 75. The protease functioned at an optimum pH of 7.0 and an optimum temperature of $50^{\circ}C$. It was also noted that the protease demonstrated a proteolytic activity of 1,756 U/mg toward casein. The $K_m$ of the purified protease toward casein was 6.36 mg/ml at a pH of 7.0 and with a temperature of $37^{\circ}C$, whereas the $V_{max}$ was 9.11 ${\mu}g\;ml^{-1}\;min^{-1}$. The activity of the protease was adversely affected by EDTA-2Na, suggesting that it is a metalloprotease. PMSF, EGTA, aprotinin, and leupeptin exerted no striking inhibitory effect. The activity of the protease was enhanced by $Fe^{2+}$, but was curtailed by $Cd^{2+}$, $Cu^{2+}$, $Hg^{2+}$, $Pb^{2+}$, $Zn^{2+}$, and $Fe^{2+}$ ions. The protease also exhibited inhibitory activity against HIV-1 reverse transcriptase with an $IC_{50}$ value of 4.00 ${\mu}M$. The $IC_{50}$ values toward hepatoma Hep G2 and leukemia L1210 cells in vitro were 4.99 ${\mu}M$ and 3.67 ${\mu}M$, respectively.

• The Korean Journal of Mycology
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• v.41 no.2
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• pp.127-131
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• 2013

Traditional knowledge on wild mushrooms in Gyeongnam province near of Hallyeo-Haesang National Park was investigated. It was interviewed from 145 of people in age of 50th's to 90th's living in 53 villages. Total 19 mushrooms were used for food in which Ramaria botrytis was the most frequently used and followed Macrolepiota procera and Lentinula edodes. L. edodes was uniquely both medicinal and edible. Chlorophyllum neomastoidea, poisonous mushroom, was used as an insect repellent. The most various mushrooms were found in Irun, Geoje where 10 species of mushrooms were used and followed Jingyo, Hadong where 8 species were used. Among the interviewed people, the number of female was more than double compared to that of male and most of the interviewed people were 70th's and 80th's in age.

• Journal of Life Science
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• v.17 no.10
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• pp.1387-1393
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• 2007

Hambag mushroom is edible mushroom, and one of new strains in Grifola frondosa. It was developed from Korean wild Grifola frondosa by National Institute Science & Technology. In this study, we investigated the effects of the powder extracted from fruit-body of hambag mushroom on hyperglycemia, hyperlipemia and pancreatic cells in rats with streptozotocin (STZ) and high fat diet-induced diabetes. The diabetic rats were fed with hambag mushroom-powder (1 and 2% of diet weight) for 6 weeks. And then the levels of blood glucose and lipid and the pancreatic tissue were analyzed and compared. The diabetic rats fed with hambag mushroom-powder lost less body weight than those fed with the powder-free normal diet. flood glucose levels in the diabetic rats fed with hambag mushroom-powder at all concentrations was lower by 50% than in the diabetic rats fed with normal diet. The levels of total cholesterol, triglyceride and LDL-cholesterol in the diabetic rats fed with hambag mushroom-powder were increased compared to those in the diabetic rats fed with normal diet. There were the blood levels of cholesterol, triglyceride and LDL-cholesterol by hambag mushroom-powder concentrations in a dose-dependent response. The decreased damage of pancreatic tissue in the group treated with hambag mushroom-powder could be suggested possibly as one of mechanisms for the effects of hambag mushroom-powder on hyperglycemia and hyperlipemia.

• Natural Product Sciences
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• v.20 no.2
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• pp.76-79
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• 2014

In our ongoing search for structurally interesting and biologically active metabolites from Korean wild mushrooms, bioassay-guided fractionation and a chemical investigation of the MeOH extracts of the fruiting bodies of the mushroom Naematoloma fasciculare resulted in the isolation of three ergosterol derivatives, (22E,24R)-ergosta-7,22-diene-$3{\beta}$,$5{\alpha}$,$6{\beta}$,$9{\alpha}$-tetrol (1), (22E,24R)-$5{\alpha}$,$8{\alpha}$-epidioxyergosta-6,22-diene-$3{\beta}$-ol 3-O-${\beta}$-$\small{D}$-glucopyranoside (2), and (22E,24R)-$5{\alpha}$,$8{\alpha}$-epidioxyergosta-6,9,22-triene-$3{\beta}$-ol 3-O-${\beta}$-$\small{D}$-glucopyranoside (3). The structures of 1 - 3 were determined by comparison of their spectroscopic and physical data with reported values. The isolated steroid derivatives 1 and 3 were reported for the first time from this mushroom. Compounds 1 - 3 were tested for their cytotoxic activities against four human cancer cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-15).

• YAKHAK HOEJI
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• v.48 no.5
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• pp.261-265
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• 2004

In the previous report, we described the marked antitumor and immunomodulatory activities of PVp, a protein-polysaccharide fraction of a Korean wild mushroom Psathyrella velutina. In this study, a protein-polysaccharide fraction, PVMP, was prepared from the shake-cultured mycelia of the same mushroom and its immunoactivities as well as chemical compositions were investigated. At 200 $\mu\textrm{g}$/ml, PVMP weakly stimulated the BALB/c mouse splenic lymphocytes to form lymphoblasts and upregulated the expression of CD25 molecules, but failed to stimulate peritoneal macrophages. In chemical analysis these two protein-polysaccharide fractions were found to be quite different in that the carbohydrate contents of PVMP and PVP, respectively, was 85.3% and 41.2%. These results reveals that PVMP, unlike PVP, is a moderate immunostimulator on the immune system.

• Preventive Nutrition and Food Science
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• v.22 no.2
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• pp.124-130
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• 2017

This paper describes in detail proximate composition, nutritional profile, phytochemical constituents, antioxidant activities, antimicrobial potential, and antihemolytic activity (towards human erythrocytes) of various fractions of wild Ganoderma lucidum. Proximate analysis established that wild G. lucidum comprises about $87.02{\pm}5.45%$ of moisture, and the remaining part is a rich source of proteins ($8.59{\pm}0.37%$), crude fiber ($54.21{\pm}1.2%$), and carbohydrate (35.16%) with smaller fat content (3.33 %). Similarly, phytochemical screening revealed the presence of flavonoids ($217.51{\pm}0.30mg/g$), ascorbic acid ($116{\pm}7.32mg/g$), phenolics ($360.72{\pm}34.07mg/g$), ${\beta}$-carotenes ($0.42{\pm}0.04{\mu}g/g$), and lycopene ($0.05{\pm}0.00{\mu}g/g$). Extracts of wild G. lucidum in various solvents provided first line protection against Escherichia coli and Pasteurella multocida in the order of ethyl acetate> ethanol> methanol> n-hexane> water. Furthermore, aqueous and methanolic extracts of wild G. lucidum were found to be safe towards human erythrocytes. Overall, wild mushroom (G. lucidum) was found to be a good source of dietary supplements, antimicrobial and antioxidant agents in the pursuance of its commercial utilization in food and pharmaceutical industries.

• The Korean Journal of Mycology
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• v.43 no.3
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• pp.142-148
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• 2015

In this study, among the wild mushrooms collected on Jeju Island for the recently 9 years, 5 species are newly reported to Korea. They are identified as Ramariopsis subtilis, Coltriciella pusilla, Lactifluus ochrogalactus, Microstoma insititium and Morchella semilibera based on macromorphological and microscopic observation. In addition, Lactifluus ochrogalactus and Microstoma insititia were re-identified by analysis of internal transcribed spacer of ribosomal DNA.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.285-294
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• 2021

In general, mycoviruses remain latent and rarely cause visible symptoms in fungal hosts; however, some viral infections have demonstrated abnormal mycelial growth and fruiting body development in commercial macrofungi, including Lentinula edodes. Compared to other cultivated mushrooms, L. edodes is more vulnerable to viral infections as it is still widely cultivated under near-natural conditions. In this study, we investigated whether Korean wild strains of L. edodes were infected by RNA mycoviruses that have previously been reported in other parts of the world (LeSV, LePV1, LeV-HKB, LeNSRV1, and LeNSRV2). Using specific primer sets that target the RNA-dependent RNA polymerase genes of each of the RNA mycovirus, reverse transcription-polymerase chain reaction (RT-PCR) was used to detect viral infection. Viral infection was detected in about 90% of the 112 wild strains that were collected in Korea between 1983 and 2020. Moreover, multiple infections with RNA mycoviruses were detected in strains that had normal fruiting bodies. This work contributes to our understanding of the distribution of RNA mycoviruses in Korea and the impact of multiple viral infections in a single strain of L. edodes.

• Communications for Statistical Applications and Methods
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• v.8 no.3
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• pp.739-751
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• 2001

In this paper, a sample design is studied for 2000 forestry management survey of five types forestry , tree felling, gathering of pine mushroom, growing of nut trees, growing of wild flowers, and lumbering industry. We introduce population stratification and a modified stratified cut-off sampling which deal with determination of sample size, sample allocation, and estimation of total and variance of estimator. Substitution of sample units and imputation of nonresponse units are discussed for reducing the nonsampling errors.