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Effect of Rifampicin on the Biosynthesis of Nucleic Acid in Chloroplast isolated from Chlorella ellipsoidea (Chlorella 세포에서 분리한 엽록체의 핵산합성에 미치는 rifampicin의 영향)

  • 이종삼;정희숙
    • Korean Journal of Microbiology
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    • v.24 no.3
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    • pp.276-287
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    • 1986
  • Chlorella ellipsoidea were cultured in the media containing rifampicin for 7 days. Aliquot cells were taken out after the inoculation and at intervals during cultivation and growth rate of Chlorella cells was measured. In order to investigate the effect of rifampicin on the nucleic acid synthesis, nucleic acid and RNA polymerase were extracted from chloroplast isolated from these cells, and the contents of nucleic acid and activity of enzyme were measured to compared with those of the control. The inhibitory concentration of rifampicin on growth was 80 ppm. The DNA contents in chloroplasts isolated were decreased 60% to compared with control, whole cells were markedly decreased 70% by rifampicin. The contents of base in the RNA were decreased 46% by rifampicin in shole cell, and 77% of base contents were decreased in chloroplast. Rifampicin also inhibited the activity of RNA polymerase, therefore whole cell was decreased 10% of activity and chloroplasts were decreased 42% of activity.

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The Effect of Whole-Body Vibration Exercise on Balance, Muscle Strength and Falls Efficacy in the Elderly (전신진동운동이 노인의 균형, 근력 및 낙상효능감에 미치는 영향)

  • Kim, Young-Min;Park, Jin-Hwan
    • Journal of the Korean Society of Physical Medicine
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    • v.12 no.4
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    • pp.61-71
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    • 2017
  • PURPOSE: The purpose of the present study was to investigate the effects of whole body vibration exercise on balance, muscle strength and falls efficacy in the elderly. METHODS: In this blinded randomized allocation study, 27 elderly were assigned to a whole-body vibration exercise group (n=14), consisting of 25 min structured exercises for 2 days per week for 6 weeks and a control group (n=13) performing the same program without vibration. At baseline and after the 6-week intervention, balance was measured using the Korean version of the Berg balance scale (K-BBS), timed up and go (TUG) test and functional reach test (FRT). Muscle strength was determined using the 30-s chair stand test (CST). Fear of falling was assessed using the Korean version of falls efficacy scale (K-FES). Paired t-test and independent t-test were used for within and between group comparisons, respectively. RESULTS: After the intervention, the experimental group showed significantly higher changes in all the parameters (K-BBS score, TUG test, CST, K-FES score) (p<.05) compared with the control group. However, there was no significant change in all parameters in the control group (p>.05). CONCLUSION: The whole-body vibration exercise program may be helpful to improve balance, mobility, muscle performance and fear of falling in the elderly.

Effect of Stage of Growth and Cultivar on Chemical Composition of Whole Maize Plant and Its Morphological Fractions

  • Firdous, R.;Gilani, A.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.3
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    • pp.366-370
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    • 1999
  • Samples of whole plant, leaf and stem of Akbar, Neelum, UM-81 and lZ-31 cultivars of maize fodder harvested up to 14 weeks at different growth stages were drawn and analysed for dry matter contents and various cell wall constituents such as NDF, ADF, hemicellulose, cellulose, lignin, cutin and silica. The dry matter contents of whole maize plant, leaf and stem increased significantly (p<0.01) with advancing plant age. Maximum dry matter was found in the leaf fraction of the plant. The cell wall components continued to increase significantly (p<0.001) in whole maize plant and its morphological fractions as the age advanced. Maximum values for NDF, ADF, cellulose and lignin were observed in stem followed by whole plant and leaf, whereas hemicellulose, cutin and silica contents were higher in leaf fraction of the plant. The cultivars were observed to have some effects on chemical composition of all plant fraction. The results indicated that maturity had a much greater effect on the concentration of all the structural components than did the cultivars. It was concluded that maize fodder should be cut preferably between 8th to 9th week of age (flowering stage) to obtain more nutritious and digestible feed for livestock. Among the maize cultivars, Neelum proved to be the best, due to its higher dry matter contents and lower lignin concentration.

Effect of Stage of Maturity and Cultivars on the Digestibility of Whole Maize Plant and its Morphological Fractions

  • Firdous, R.;Gilani, A.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.8
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    • pp.1228-1233
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    • 1999
  • A study was conducted on four maize cultivars to determine the dry matter and fibre digestibility as influenced by advancing plant age. Samples of maize cultivars Akbar, Neelum, UM-81 and IZ-31 were harvested at weekly intervals/ growth stages. The samples of morphological fractions such as leaf and stem were also collected at various growth stages. Whole mixed fodder and different fractions of maize plant were analysed for their chemical composition and in vitro digestibility. The results showed that in vitro dry matter digestibility (IVDMD) of whole maize plant, leaf and stem decreased significantly with advancing stage of maturity. Digestibility of NDF, ADF, hemicellulose and cellulose decreased significantly in all plant parts with advancing plant age/growth stages. Maximum values for the digestibility of dry matter and various cell wall constituents were observed in leaf, followed by whole plant and stem fractions. Cultivars were observed to have significant effect of IVDMD and digestibility of NDF, ADF and cellulose in all plant fractions. The results indicated that digestibility of maize fodder was affected by stage of maturity and cultivars. However, maturity had a greater effect on digestibility in all plant fractions than did cultivars. Dry matter contents were found to be significantly and negatively correlated with IVDMD of whole plant and its leaf and stem fractions. Based on correlations, regression equations were computed to predict IVDMD.

In Vivo Immunopotentiating Effects of Cellular Components from Lactococcus lactis ssp. lactis

  • Kim Ji-Yeon;Lee Seong-Kyu;Jeong Do-Won;Hachimura Satoshi;Kaminogawa Shuichi;Lee Hyong-Joo
    • Journal of Microbiology and Biotechnology
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    • v.16 no.5
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    • pp.786-790
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    • 2006
  • Cellular components of Lactococcus lactis ssp. lactis (heat-killed whole cells, cytoplasm, and cell walls) were tested for their in vivo immunopotentiating activity. Peritoneal macrophages from mice orally administered with heat-killed whole cells exhibited significantly greater phagocytic activity than the groups administered with cell-wall fraction or cytoplasm fraction. The cytotoxicity of natural-killer cells was the highest in the group administered with whole cells, and the production of cytokines ($IFN-\gamma$, IL-2, and IL-12) in spleen cells was significantly higher, when cellular components were injected, and it tended to be higher in the cell-wall and cytoplasm groups than in the whole-cell group. Interestingly, the cytokine production of Peyer's patch cells was high, when cytoplasm fractions were administered. These results demonstrate that whole cells and cytoplasm and cell-wall fractions of L. lactis ssp. lactis have immunopotentiating activities, which are related to the stimulation of Peyer's patches.

A Study of The Comparison between Mothers' and Teachers' Beliefson Children's Literacy Development and Their Literacy Practices (문해습득에 대한 어머니와 교사의 신념과 문해활동 비교 분석 연구 - 총체적 언어접근을 중심으로)

  • Song, Seung-Min
    • Korean Journal of Human Ecology
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    • v.18 no.5
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    • pp.1035-1046
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    • 2009
  • The study was designed to explore mothers' and teachers' beliefs on children's literacy development by comparing their beliefs with the whole language approach. Also, their literacy practices at home and in the classroom were compared and how their literacy beliefs and practices were related was investigated. 176 mothers and 72 daycare teachers participated in this study in Kyunggi-do. Mean, standard deviation, frequency, t-test, and multiple regression were employed to analyze the data. The results showed that the mothers' age, mothers' education, fathers' education and family income mostly predict the mothers' literacy beliefs. Also the teachers' age, education, and their learning experiences with the whole language approach are the main predicting variables in the teachers' beliefs. In practices, the mothers' age, number of children, and fathers' education are independent variables predicting the mothers' practices. The teachers' age, education, interest about the whole language approach, learning experience, and number of children in the classroom are independent variables into the teachers' practices. Overall, teachers showed a higher level of beliefs on the whole language approach and did more literacy practices than the mothers.

Study for the Cause of Death in the Life Insurance Polices Standard Risk (표준체(標準體) 보험계약(保險契約)의 사인(死因)에 대한 통계적(統計的) 고찰(考察))

  • Ko, Choul-Soo;Kim, Kang-Sueck
    • The Journal of the Korean life insurance medical association
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    • v.1 no.1
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    • pp.140-147
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    • 1984
  • We've reviewed 5,067 cases of claims by death that had occured for about 5 years from January, 1979 to October, 1983. As a result, We came to following conclusions; First, the greater part of deaths above mentioned were due to the Disease of Adult such as the Malignant neoplasm(18.4%), a Heart disease(10.4%), Liver cirrhosis(9.1%) and the Cerebrovascular disease(7.6%) as well as Accidental Death(26.5%), which were occupied by 72% of the whole. Second, classifying them by medical examination or non-medical, deaths in case of non-medical examination showed 80.8% of the. whole. And for age, sections ranging from 40 to 49 and from 30 to 39 took the overwhelming portions by 36.9% and 29.8% respectively, both of which showed 66.7% of the whole. Third, for the period elapsed, deaths within 1 year from the entrance showed 21.3% and that from 1 years to under 2 years, 19.9%. Thus the rate of early death under 2 years stood for 41.2% of the whole. Fourth, for occupation of the insureds, commerce occupied 20.4%, company employees 14.4%, agriculture 13.3%. These three categories marked 48.8% of the whole. From this, it appeared that the accidental death rate of the insured with the risky occupation was much greater than orthers.

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New Korean Traditional Papermaking From Morus spp.(II) -Properties of the Hanjis Made from Bast Fiber and Whole Stalk Fiber- (뽕나무를 이용한 새로운 한지의 제조(제 2 보) -인피부 및 전간부 섬유.한지의 특성)

  • 최태호;조남석
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.31 no.4
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    • pp.84-92
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    • 1999
  • Excellence of the Korean traditional paper(Hanji) can be proved by literatures and legacies handed down from generations. However, with the problems pertaining to a majority of traditional manufactures, Hanji industry was declined because of low productivity, insufficient supply of raw material , and reduced demand. therefore, modernizxation of the Hanji technology and development of new uses are very important. This study was carried to investigated the papermaking characteristics of Morus spp.(M.alba, M.Bombycis, and M.Ihou) for new Hanji, modernize manufacturing process of Hanji by grafting on developed modern pulping and papermaking technology, develop the various uses, and establish the foundation for development of high value-added products. Hanji is made from M.Ihou, while stalk , and sulfomethylated pulp gave better sheet formation and higher brightness than those of the others. Physical properties of Hanji which made from bast fiber pulps were better than those of whole stalk pulps. Hanji which made from M.bombycis bast fiber and whole stalk pulps were not so good sheet strength as other species. Sulfomethylated whole stalk pulps were shown better sheet strength than alkali and alkali-hydrogen peroxide whole stalk pulps.

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Study on The Development of A New Whole Body Fame

  • Chung, Jin-Bum;Suh, Tae-Suk;Chung, Won-Kyun;Choe, Bo-Young;Lee, Hyoung-Koo
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.136-137
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    • 2002
  • We have been researching upgrade version of a stereotactic whole body frame, used for evaluating daily setup accuracy of the patient positioning during fractionated extra-cranial stereotactic radiotherapy. Currently, we are focusing on the development of a new stereotactic whole body frame, and then will handle organ movement produced by breathing at the next stage. MeV-Green is chosen for the best immobilizer possible and the epoxy board is for the frame with the dimension of 110 em in length, 50 cm in width in order to maximize transmission rate of the beam from lateral or posterior direction and to fit CT and PET scanners with an aperture of 55 cm at least. The key point of an upgraded stereotactic whole body frame will be set on the collision-free rotation of the gantry with the frame, and the development of the checking structure for the daily patient repositioning regarding internal target.

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Identification of Discrimination Factors for a Pig Noncontact Weighing System Using Image Data (영상정보를 이용한 돼지의 비접촉 체중계측시스템 인자 구명)

  • 장동일;임영일;임정택;장요한;장홍희
    • Journal of Animal Environmental Science
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    • v.5 no.2
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    • pp.93-100
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    • 1999
  • Pig's original image data was transformed to a binary image, an image excluding head and tail portion from the whole binary image, and a projected image associated with pig's height. Then the length of body, width of shoulder, and area of pig were calculated and the relationships among the above characteristics and pig's weight were analyzed. The results obtained from this study were as follows: 1. Whole binary image data was considered to be improper to determine the pig's weight because the movement of pig's head and tail portion affected the image data. 2. Binary image data excluding head and tail portion from the whole binary image showed a better estimation of the pig's weight than the whole binary image. 3. Pig's should width was analyzed to be improper factor to determine the pig's weight. 4. The projected image associated with pig's height showed the highest correlation between the pig's area of the image and pig's weight(R2=0.9965). From this research the projected image associated with pig's height, which is excluding head and tail portion from the whole body of pig's image, was considered to be the prime factor to measure the pig's weight by the noncontact measurement.