• Animal Bioscience
• /
• v.35 no.7
• /
• pp.1069-1079
• /
• 2022

Objective: The objective of this study was to investigate the effect of 4-month intensive feeding on the meat quality, fatty acid profile, flavor, and growth performance of grazing Hulunbuir sheep (HBS). Methods: The HBS were selected 4-months after birth in a pasture rearing system as the experimental animals (n = 44, female, average body weight 23.8±2.2 kg) then divided equally into pasture-based grazing fattening (PAS) and concentrate-included intensive fattening (CON) groups for another 4-month finishing. When finished fattening, all animals were slaughtered to collect musculus longissimus dorsi subcutaneous adipose tissue and to investigate the influences on meat quality, fatty acid profile, flavor and growth performance. Results: The results showed lambs in CON group got significantly higher live weight, hot carcass weight, and dressing percentage. The CON group had significantly higher value of redness (a^*), lightness (L^*) and water holding capacity (p<0.05), significantly lower value of Warner-Bratzler shear force than the PAS group (p<0.05). The subcutaneous fat from CON group lambs demonstrated a significantly higher content of C18:1 and C18:2 (p<0.05), but lower C14:0 and C16:0, indicating an increased degree of unsaturated fatty acid. The content of 4-methyloctanoic acid, 4-ethyloctanoic acid and 4-methylnonanoic acid had increased 2 to 4 times, representing a more intense odor in the CON group. However, the values were still lower than most sheep breeds reported, indicating the indoor feeding system could not fundamentally deteriorate the excellent meat characteristic of HBS. Conclusion: It was evident that lambs in CON group exhibited a better meat production performance, improved in meat color, texture and healthier fatty acid profile through pasture-weaned concentrate included intensive fattening system, which offers a good alternative regimen for lamb finishing and has a wide prospection in the HBS meat industry.

• Analytical Science and Technology
• /
• v.35 no.5
• /
• pp.205-211
• /
• 2022

Deuterium (D) is an isotope with one more neutron number than hydrogen (H). Heavy elements rarely change their chemical properties with little effect even if the number of neutrons increases, but low-mass elements change their vibration energy, diffusion rate, and reaction rate because the effect cannot be ignored, which is called an isotope effect. Recently, in the semiconductor and display industries, there is a trend to replace hydrogen gas (H₂) with deuterium gas (D₂) in order to improve process stability and product quality by using the isotope effect. In addition, as the demand for D₂ in industries increases, domestic gas producers are making efforts to produce and supply D₂ on their own. In the case of high purity D₂, most of them are produced by electrolysis of heavy water (D₂O), and among D₂, hydrogen deuteride (HD) molecules are present as isotope impurities. Therefore, in order to maximize the isotope effect of hydrogen in the electronic industry, HD, which is an isotope impurity of D₂ used in the process, should be small amount. To this end, purity analysis of D₂ for industrial processing is essential. In this study, HD quantitative analysis of D₂ for high purity D₂ purity analysis was established and hydrogen isotope RM (Reference material) was developed. Since hydrogen isotopes are difficult to analyze with general gas analysis instrument, they were analyzed using a high-precision mass spectrometer (Gas/MS, Finnigan MAT271). High purity HD gas was injected into Gas/MS, sensitivity was determined by a signal according to pressure, and HD concentrations in two bottles of D₂ were quantified using the corresponding sensitivity. The amount fraction of HD in each D₂ was (4518 ± 275) μmol/mol, (2282 ± 144) μmol/mol. D₂, which quantifies HD amount using the developed quantitative analysis method, will be manufactured with hydrogen isotope RM and distributed for quality management and maintenance of electronic industries and gas producers in the future.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.30 no.2
• /
• pp.29-42
• /
• 2022

The government promotes the 2050 carbon-neutral policy. Therefore, the concern to convert livestock manure into energy is increasing for the reduction of greenhouse gases generated in the livestock industry sector. In this study, the economic feasibility of the livestock manure solid fuel power generation facility, which is a major consumer of livestock manure solid fuel, was assessed to expand the demand for livestock manure solid fuel. The production cost of livestock manure solid fuel showed the lowest production cost of 97.4 thousand won/ton when dried using solid fuel at a 200 ton/day scale bio-drying facility. The livestock manure solid fuel power generation facility showed economic feasibility at a REC weight of 1.5 in the case of the bio-drying facility, so it was necessary to set a REC weight of 1.5 or more to expand the demand for livestock manure solid fuel. The conversion of livestock manure into solid fuel has various environmental benefits, such as the reduction of greenhouse gases and the effect of reducing non-point pollutants in the water system. Therefore, in order to expand livestock manure solid fuel production facility, it was required to review the feasibility including various environmental benefits.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.58 no.1
• /
• pp.39-48
• /
• 2022

Using environmental DNA (eDNA) in the fisheries and oceanography fields, research on the diversity of biological species, the presence or absence of specific species and quantitative evaluation of species has considerably been performed. Up to date, no study on eDNA has been tried in the area of fisheries acoustics in Korea. In this study, the biomass of a dominant species in the northwestern waters of Jeju Island was examined using 1) the catch ratio of the species from trawl survey results and 2) the ranking ratio of the species from the eDNA results. The dominant species was Zoarces gillii, and its trawl catch ratio was 68.2% and its eDNA ratio was 81.3%. The Zoarces gillii biomass from the two methods was 7199.4 tons (trawl) and 8584.6 tons (eDNA), respectively. The mean and standard deviation of the acoustic backscattering strength values (120 kHz) from the entire survey area were 135.5 and 157.7 m²/nm², respectively. The strongest echo signal occurred at latitude 34° and longitude 126°15' (northwest of Jeju Island). High echo signals were observed in a specific oceanographic feature (salinity range of 32-33 psu and the water temperature range of 19-20℃). This study was a pilot study on evaluating quantitatively aquatic resources by applying the eDNA technique into acoustic-trawl survey method. Points to be considered for high-quality quantitative estimation using the eDNA to fisheries acosutics were discussed.

• Journal of Applied Biological Chemistry
• /
• v.65 no.3
• /
• pp.173-181
• /
• 2022

This study was carried out to investigate the residual characteristics of flubendiamide in kale to establish pre-harvest residue limits (PHRL) and the removal efficiency according to the washing solvent and method. Field tests were conducted at two different greenhouses, field 1 (Anseong-si, Gyeonggi-do) and field 2 (Incheon-si, Gyeonggi-do). According to the safe use guidelines kale was sprayed with flubendiamide twice every 10 days and harvested 0 (after 2 h), 1, 2, 3, 5, 7 and 10 days after the final application. The biological half-live of flubendiamide in kale was calculated based on dissipation curves of the pesticide in samples analyzed by liquid chromatography coupled with tandem mass spectrometry. In the analysis, method limits of quantitation (MLOQ) were 0.01 mg/kg, and recoveries performed with two different fortification levels of 10 MLOQ and maximum residue limit (0.7 mg/kg) were 104.2±3.6 and 101.9±10.2%, respectively. The dissipation rate constant of flubendiamide in kales were 0.2437 at field 1 and 0.1981 at field 2. PHRL calculation equations obtained using the dissipation constants estimated as follows: if the residual concentration of flubendiamide in kale on 10 days before harvest is less than 8.0 mg/kg, the residual concentration on the harvest would be under MRL. The removal of flubendiamide from kale was the greatest when it was washed with vinegar (39.8%), followed by baking soda (31.7%), calcium powder (30.2%), neutral detergent (27.2%), and tap water (15.9%). The results of this study would be useful for both farmers and consumers to produce or consume safe agricultural products.

• Korean Journal of Ichthyology
• /
• v.34 no.2
• /
• pp.96-101
• /
• 2022

The egg, larvae, and juvenile development of Platycephalus indicus sampled from Yeosu estuary were conducted. The egg shape of P. indicus is spherical and transparent on the outside, with two perivitelline cavities inside and one oil globule. The diameter of the fertilized eggs were 1.03~1.12 mm (mean =1.08 mm, n =50). The embryos hatched in about 50 hrs 30 mins after fertilization at the water temperature of 20℃. The newly hatched larvae showed a total length of 2.72~3.04 mm (mean=2.93±0.21 mm, n=50). At 5 days after hatching, they were 3.88~4.42 mm (mean=4.11±0.31 mm, n=15) in TL and their yolk was completely absorbed, developing the teeth. They became juvenile 39 days after hatching and reached 10.23~11.95 mm (mean=11.09±0.86 mm, n=5) in TL. At 45 days after hatching, they were 12.01~13.25 mm (mean=12.63±0.62 mm, n=5) in TL, and their body shape and color were similar to those of adult fish.

• Journal of fish pathology
• /
• v.35 no.1
• /
• pp.57-63
• /
• 2022

Vibrio parahaemolyticus and V. vulnificus are known to be infected to human via fisheries products. Therefore, food safety of fisheries products is important for public health and fish industry. This paper was conducted to know how well these human isolates can survive in olive flounder (Paralichthys olivaceus). The growth of V. parahaemolyticus and V. vulnificus showed about 50~60% reduced rates at 25℃ than at 37℃ and did not show any differences according to NaCl concentration of media except the increasing in the growth of V. vulnificus in medium containing 3% NaCl. Artificial infection of 1×10⁶ CFU/fish was carried out to confirm the sensitivity of olive flounder against V. parahaemolyticus and V. vulnificus. After 1 week from injection, no fish was dead. To evaluate nonspecific defense of olive flounder against V. parahaemolyticus and V. vulnificus, the antibacterial potency of serum and epidermal mucus were tested. The number of the vibrios exposed to serum obtained from olive flounder significantly decreased after 3 hours, and epidermal mucus showed decrease of the bacteria over than 90% until 12 hours from exposure. Phagocytosis of head kidney leucocytes of healthy olive flounder against V. parahaemolyticus and V. vulnificus showed in over 70% of leucocytes at the 2 hours. Therefore, cultured olive flounder only as vehicle for human pathogen in environmental water is well developed its antibacterial potency against human pathogens, so the viability of V. parahaemolyticus and V. vulnificus in cultured olive flounder was considered very low.

• Journal of Life Science
• /
• v.33 no.1
• /
• pp.73-81
• /
• 2023

In the present study, we prepared hot water extracts and the subsequent organic solvent fractions of methanol extracts of Houttuynia cordata (HC) and Lespedeza cuneata (LC), and investigated their anti-inflammatory effects on lipopolysaccharide-stimulated RAW264.7 cells. Among the treated samples, hexane, chloroform, and ethyl-acetate fractions of HC and LC inhibited nitric oxide (NO) production in a dose-dependent manner, and decreased inducible nitric oxide synthase (iNOS) protein expression. And, we analyzed the flavonoid contents of the ethyl-acetate fraction of HC and LC, and chose apigenin for the further experiments because apigenin was one of flavonoids commonly found in HC and LC. Apigenin dramatically inhibited NO production in a dose-dependent manner without affecting cell viability and decreased iNOS and cyclooxygenase-2 (COX-2) expression. In addition, apigenin suppressed the phosphorylation of p38 and Jun N-terminal kinase (JNK) indicating that apigenin exerts anti-inflammatory activity via the mitogen-activated protein kinase (MAPK) signaling pathway. Subsequently, we conducted RNA-sequencing analysis to detect differentially expressed genes upon apigenin treatment. Among the down-regulated genes, four cytokine genes (interleukin (IL)-1α, IL-1β, IL-6, and colony stimulating factor 2 (CSF2)) were selected for the further analysis, and the reduction of their expression by apigenin was confirmed with quantitative real-time polymerase chain reaction. Overall, our results suggest that Houttuynia cordata and Lespedeza cuneata have the anti-inflammatory effects and apigenin can be the one of key molecules responsible for their anti-inflammatory activities.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
• /
• v.29 no.2
• /
• pp.95-102
• /
• 2023

Despite the easing of social distancing, demand for non-face-to-face services continues to rise. Recently, the EU is pursuing a comprehensive plastic use reduction by expanding the scope of plastic use regulations for packaging plastics according to the New Cyclical Economy Action Plan(NCEAP). In response to this trend, the packaging industry is moving away from conventional non-degradable/petroleum-based plastics and conducting research on packaging materials using biodegradable plastics such as PLA(Poly Lactic Acid), PBAT(Poly Butylene Adipate-co-butylene Terephthalate). On the other hand, ginkgo leaves occur in large quantities in Korea and act as a cause of slip accidents and flooding. In this study, a method to utilize ginkgo biloba leaf as a new alternative biomass resource was proposed by producing lactic acid through pretreatment, enzymatic saccharification, and fermentation processes. For the efficiency of lactic acid production, a comparative analysis of lignin content from before and after browning was performed. In addition, the degree of glucan extraction was evaluated by applying a pretreatment method using three catalysts: hot water, sulfuric acid, and sodium hydroxide. It is difficult to expect high production of lactic acid with single process. Therefore, an integrated process operation using both the pretreated hydrolyzate and the residual solid enzymatic saccharification solution must necessarily be applied.

• Korean Journal of Mineralogy and Petrology
• /
• v.36 no.2
• /
• pp.107-115
• /
• 2023

Cobalt can be released into the natural environment as industrial waste from the alloying industry and as acid mine drainage, and it is also a radionuclide (⁶⁰Co) that constitutes high-level radioactive waste. Smectite is a mineral that can be useful for adsorption and isolation of this element. In this study, Cheto-type montmorillonite (Cheto-MM), which is the source clays of The Clay Mineral Society (CMS) and already well-characterized, was used. The effect of the adsorption site affected by the presence of interlayer water on the adsorption of cobalt before and after dehydration by heating was evaluated and the adsorption mechanism of cobalt on Cheto-MM was studied by applying adsorption kinetics and adsorption isotherm models. The results showed that the adsorption characteristics changed with dehydration and subsequent shrinkage, and cobalt was found to be adsorbed at the edge of Cheto-MM for about 38% and adsorbed at the interlayer site for about 62%, suggesting that the cobalt adsorption of Cheto-MM is significantly influenced by the interlayer. By applying the adsorption kinetic models, the cobalt adsorption kinetics of Cheto-MM is explained by a pseudo-second-order model, and the concentration-dependent adsorption was best described by the Langmuir isotherm adsorption model. This study provides basic knowledge on the adsorption characteristic of cobalt on montmorillonite with different adsorption sites and is expected to be useful in predicting the adsorption behavior of smectite in high-level radioactive waste disposal sites in the future.