• 제목/요약/키워드: water control

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Evaluation of Proposed Diagnostic System for Detection of Pan-enterovirus Using Reverse Transcription Nested PCR from Water Environment

  • Siwon Lee;Kyung Seon Bae;Jin-Ho Kim;Ji-Hyun Park;Ji Hye Kim;Ji-Yeon Park;Kyung-Jin Lee;Chae-Rin Jeon;Jeong-Ki Yoon;Soo-Hyung Lee;Eung-Roh Park
    • Biomedical Science Letters
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    • v.29 no.2
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    • pp.81-87
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    • 2023
  • Pan-Enterovirus (Pan-EV) infects millions of children and infants worldwide every year. As severe infections have recently been reported, the need for monitoring has consequently intensified. Pan-EV is a categorical name for waterborne enteroviruses belonging to the Picornaviridae family, and includes a wide range of pathogens including Coxsackievirus (CoxV), Echovirus (EcoV) and Enterovirus (EV). In this study, we proposed an optimal RT-nested PCR method for diagnosis of various types of Pan-EV in an aquatic environment and developed a positive control. Considering detection sensitivity, specific reaction, and final identification, one condition capable of amplifying 478 bp among the four candidates in the 1st round PCR (RT-PCR) and one condition in the 2nd round PCR (nested PCR) were selected. Through the detection of nucleic acids extracted from 123 groundwater samples and the detection sensitivity test based on artificial spiking in the sample, the methods are optimal for non-disinfected water samples such as groundwater. We developed a positive control for Pan-EV detection that can be amplified to different sizes under the two conditions. Accuracy could be further improved by testing for contamination from the control group. The method proposed in this study and the positive control developed are expected to be used in monitoring Pan-EV in aquatic environments including groundwater through future research using more samples.

Traits of Water Level Control by Sluice Gates and Halophyte Community Formation in Saemangeum (새만금 배수갑문 수위조절 특성과 염생식물 군락지 형성에 관한 연구)

  • Sin, Myoung-Ho;Kim, Chang-Hwan
    • Korean Journal of Environment and Ecology
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    • v.24 no.2
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    • pp.186-193
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    • 2010
  • In order to examine the traits of sluice gate water control, halophyte community formation and their inter-relations in Saemangeum, both water level condition and halophyte community formation were analyzed periodically and spatially on the topographic map with Surfer, Saemageum Spatial Analysis System, and related field reports. The traits of water level condition are that average water level in the growing period of halophytes was similar to annual average water level, annual low level and high level appeared in the growing period, and water level was usually maintained within a range of -1.0m~0.5m above mean sea level, but it has changed more frequently year by year. Routine water level control, natural disaster prevention, construction, and civil appeal took major percentages of the reasons for sluice gate's opening and shutting. Since 2007, not only the overall control frequency of sluice gate but also its control frequency for construction and natural disaster prevention have increased outstandingly. Halophyte community had formed at a rate of 1,209ha/year in the 4,315 ha land in 2008, 6.3 times larger than in 2005, and 2,382 ha above around 1.0m was estimated to be artificially vegetated, 89.1 % of the 2,673ha-size sown area. High water level was found to be a more possible determinant than average water level or low water level in halophyte community formation and it was thought to be secondary factors whether tillage was conducted or/and whether surface sealing formed.

Prediction of Daily Water Supply Using Neuro Genetic Hybrid Model (뉴로 유전자 결합모형을 이용한 상수도 1일 급수량 예측)

  • Rhee, Kyoung-Hoon;Kang, Il-Hwan;Moon, Byoung-Seok;Park, Jin-Geum
    • Journal of Environmental Impact Assessment
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    • v.14 no.4
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    • pp.157-164
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    • 2005
  • Existing models that predict of Daily water supply include statistical models and neural network model. The neural network model was more effective than the statistical models. Only neural network model, which predict of Daily water supply, is focused on estimation of the operational control. Neural network model takes long learning time and gets into local minimum. This study proposes Neuro Genetic hybrid model which a combination of genetic algorithm and neural network. Hybrid model makes up for neural network's shortcomings. In this study, the amount of supply, the mean temperature and the population of the area supplied with water are use for neural network's learning patterns for prediction. RMSE(Root Mean Square Error) is used for a MOE(Measure Of Effectiveness). The comparison of the two models showed that the predicting capability of Hybrid model is more effective than that of neural network model. The proposed hybrid model is able to predict of Daily water, thus it can apply real time estimation of operational control of water works and water drain pipes. Proposed models include accidental cases such as a suspension of water supply. The maximum error rate between the estimation of the model and the actual measurement was 11.81% and the average error was lower than 1.76%. The model is expected to be a real-time estimation of the operational control of water works and water/drain pipes.

Coagulation Control of High Turbid Water Samples Using a Streaming Current Control System (유동흐름 전류계를 이용한 정수장 고탁도 유입수 응집 제어 방법에 대한 연구)

  • Nam, Seung-Woo;Jo, Byung-Il;Kim, Won-Kyong;Zoh, Kyung-Duk
    • Journal of Environmental Health Sciences
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    • v.38 no.2
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    • pp.128-135
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    • 2012
  • Objectives: This study was aimed at determining the optimum coagulation dosage in a high turbid kaolin water sample using streaming current detection (SCD) as an alternative to the jar test. Methods: SCD is able to optimize coagulant dosing by titration of negatively charged particles. Kaolin particles were used to mimic highly turbid water ranging from 50 to 600 NTU, and polyaluminum chloride (PAC, 17%) was applied as a titrant and coagulant. The coagulation consisted of rapid stirring (5 min at 140 rpm), reduced stirring (20 min at 70 rpm), and settling (60 min). To confirm the coagulation effect, a jar test was also compared with the SCD titration results. Results: SCD titration of kaolin water samples showed that the dose of PAC increased as the pH rose. However, supernatant turbidity less than 1 NTU after coagulation was not achieved for high turbid water by SCD titration. Instead, a conversion factor was used to calculate the optimum PAC dosage for high turbid water by correlating a jar test result with that from an SCD titration. Using this approach, we were able to successfully achieve less than 1 NTU in treated water. Conclusions: For high turbid water influent in a water treatment plant, particularly during summer, the application of SCD control by applying a conversion factor can be more useful than a jar test due to the rapid calculation of coagulation dosage. Also, the interpolation of converted PAC dose could successfully achieve turbidity in the treated water of less than 1 NTU. This result indicates that an SCD system can be effectively used in a water treatment plant even for high turbid water during the rainy season.

Microbiological Pollution Investigation of Spring water in Seogwipo city of Jeju Island (제주도내 서귀포시 해안가 저지대용천수의 미생물학적 오염도 조사)

  • Han, Yong-Jae;Kim, Man-Chul;Moon, Yung-Gun;Heo, Moon-Soo
    • Proceedings of the KAIS Fall Conference
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    • 2010.11b
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    • pp.1025-1028
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    • 2010
  • The bacteriological examination of spring water in Seogwipo-city was conducted. A total 11 spring water samples were performed from January to April, 2010. During the study period, the range of temperature was from 0.7 to $15.4^{\circ}C$, and result of the analyses showed that hydrogen ion concentrations (pH) for spring water was 0.33 to 7.8. salinity levels for sample average of 0.5 to 1.04‰, as the result of measuring dissolved oxygen (DO) for spring water showed that water dissolved oxygen were 1.02 to 7.14 mg/${\ell}$. The range of total coliform of spring water sample at 11 stations located in the designated spring water were <1.8~>1,600 MPN/100mL. And the range of geometric mean of total coliform were 1.9~117.1 MPN/100mL, The range of fecal coliform of spring water sample at 11 stations located in the designated spring water were <1.8~>1,600 MPN/100mL. And the range of geometric mean of fecal coliform were 1.8~68.1 MPN/100mL, respectively. Level of microbial contamination was examined in 11 samples for indication of bacterial contamination such as heterophic bacteria, EscherichiacoliO157;H7, salmonella spp., Vibrio parahaemolyticus, Staphylococcus aureus, Shigella spp. Were frequently detected from the spring water. Salmonella spp., S.aureus were detected in the range of $0{\sim}1.0{\times}10^1$, $0{\sim}3.0{\times}10^1$ CFU/ml, respectively. And the Escherichia coli O157;H7, Vibrio parahaemolyticus, Shigella spp. Were not detected from the examined spring water samples.

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Classification of small irrigation ponds in western Civilian Control Zone in Korea (서부 민간인 통제구역에 존재하는 둠벙의 유형분류)

  • Kim, Seung-Ho;Kim, Jae-Hyun;Kim, Jae-Geun
    • Journal of Wetlands Research
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    • v.13 no.2
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    • pp.275-289
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    • 2011
  • We investigated the hydrological and geomorphological characteristics of small irrigation ponds in civilian control zone of Paju city in Korea. Among 85 small irrigation ponds, water level of 52 ponds changed seasonally and that of 33 was constant. Water sources of 12 ponds were surface water, 29 surface water and ground water, and 44 ground water. 4 ponds locate in the edges of forests, 33 in flat-lands, and 48 in valleys. Water in 45 ponds was exchanged with paddy fields and 40 ponds were isolated from paddy fields. Endangered or endemic species were inhabited in 26 ponds, which have ground water as water source and constant water level. Based on these characteristics, we suggested 4 types of small irrigation ponds: spring, water exchanging, stagnant/spring, stagnant water. This classification system will help ecosystem managers to investigate ponds systematically and manage them based on pond type.

Research on the Development Management Basin and Goal for 3th T.W.Q on the Boundary between Metropolitan Cities/Dos Specified in Nakdong River Basin (낙동강수계 3단계 광역시·도 경계지점 목표수질 설정을 위한 관리권역 및 관리목표 설정 방법 연구)

  • Hwang, Ha Sun;Park, Ji Hyung;Kim, Yong Seok;Rhew, Doug Hee;Choi, Yu Jin;Lee, Sung Jun
    • Journal of Korean Society on Water Environment
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    • v.31 no.5
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    • pp.569-575
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    • 2015
  • The current Total Pollution Load Control (TPLC) sets the Target Water Quality (TWQ) by utilizing the delivery ratio, unit loads, and water quality modeling, it also allocates the watershed's permitted discharge load. Currently, common target pollutants of every unit watershed in TPLC are BOD and T-P. This study has reviewed the 1th and 2th of TWQ setting process for the Nakdong River 3th TWQ setting in Total Pollution Load Control (TPLC). As a result of review, 1th and 2th were divided into one management basin (mulgeum) for setting management goals. However, 3th was divided into six management basins (mulgeum, gnagjeong, geumho river, nam river, miryang river, end of nakdong river). The principle of management goal setting were to achieve the objective criteria of Medium Areas for the linkage of the water environment management policy. And Anti-Degredation (principle of preventing deterioration) were applied to the 3th TWQ. Also, additional indicators were considered in accordance with the reduction scenarios for the final management goals.

Evaluation of Water Absorption Speed for Litter Materials to Improve the Water Control Ability of Livestock Litter (우사의 수분조절능력 향상을 위한 깔짚소재별 수분흡수속도 평가)

  • Rho, Jun-Suk;Lee, Jae-Hoon;Lee, Su-Lim;Park, Jong-Hwan;Seo, Dong-Cheol
    • Korean Journal of Environmental Agriculture
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    • v.41 no.1
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    • pp.24-31
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    • 2022
  • BACKGROUND: The most common litter materials used in South Korea are sawdust, rice husk, etc. Recently, the cost of litter has been steadily rising, and the maturity test has been strengthened. For this reason, new litter materials are needed for better water control ability to solve the problems. The object of this study was to evaluate the water absorption properties for litter materials. METHODS AND RESULTS: The volumetric water capacity according to the addition of cow manure was investigated to calculate the water absorption speed of litter materials (sawdust, peatmoss, cocopeat, and biochar). The water absorption speed constant (-K) in the first stage was high in the order of cocopeat (0.1190), sawdust (0.0961), biochar (0.0762), and peatmoss (0.0523). The optimal period of the litter use was in the following order: peatmoss (48d), biochar (42d), sawdust (30d), and cocopeat (24d). The water absorption rate (%) of the used litters was high in the order of biochar ≈ cocopeat, sawdust, and peatmoss, which was significantly correlated with the water absorption speed of the first stage. CONCLUSION(S): Considering the water absorption speed and water absorption rate, biochar and peatmoss were found to be the best and optimal litter materials among the tested materials. These litter materials can be used as water control agents in livestock facilities.

A Study on Daily Water Demand Prediction Model (급수량(給水量) 단기(短期) 수요예측(需要豫測)에 대한 연구(硏究))

  • Koo, Jayoug;Koizwui, Akirau;Inakazu, Toyono
    • Journal of Korean Society of Water and Wastewater
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    • v.11 no.1
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    • pp.109-118
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    • 1997
  • In this study, we examined the structural analysis of water demand fluctuation for water distribution control of water supply network. In order to analyze for the length of stationary time series, we calculate autocorrelation coefficient of each case equally divided data size. As a result, it was found that, with the data size of around three months, any case could be used as stationary time series. we analyze cross-correlation coefficient between the daily water consumption's data and primary influence factors. As a result, we have decided to use weather conditions and maximum temperature as natural primary factors and holidays as a social factor. Applying the multiple ARIMA model, we obtains an effective model to describe the daily water demand prediction. From the forecasting result, even though we forecast water distribution quantity of the following year, estimated values well express the flctuations of measurements. Thus, the suitability of the model for practical use can be confirmed. When this model is used for practical water distribution control, water distribution quantity for the following day should be found by inputting maximum temperature and weather conditions obtained from weather forecast, and water purification plants and service reservoirs should be operated based on this information while operation of pumps and valves should be set up. Consequently, we will be able to devise a rational water management system.

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Hazardous event analysis in drinking water production using aquifer storage transfer and recovery (대수층 저장·이동 및 회수에 의한 음용수 생산과정에서의 위해사건분석)

  • Lee, Sang-Il;Ji, Hyon-Wook
    • Journal of Korean Society of Water and Wastewater
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    • v.29 no.1
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    • pp.23-31
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    • 2015
  • Aquifer storage transfer and recovery (ASTR) is a type of managed aquifer recharge which entails injecting water into a storage well and recovering it from a different well. It has effects of natural purification when injected water passes through aquifer medium, and can be a good way of supplying water especially in a region with poor surface water quality. This study is about an on-going effort to introduce ASTR as a solution to source water problems in coastal areas. A pilot study is being conducted in the delta of the Nakdong River. A proactive management system is incorporated to ensure the water qulity in the process of drinking water process. The system is based on the Hazard Analysis and Critical Control Point (HACCP) which is a tool originated from the food industry in order to assess hazards and establish control systems for the safety of food product. In this paper, we analyze hazardous events which can occur in the entire water supply system using ASTR as a first step to the incorporation of HACCP to drinking water production process.